LOCUS       NZ_CP093470             4546 bp    DNA     circular CON 11-JUN-2024
DEFINITION  Klebsiella pneumoniae strain VA681 plasmid pVA681-5, complete
            sequence.
ACCESSION   NZ_CP093470
VERSION     NZ_CP093470.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26642721
            Assembly: GCF_030061665.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Complete genome sequencing and analysis of carbapenem-resistant
            Klebsiella pneumoniae isolated in Chile
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2022) Biology, Grupo de Microbiologia Integrativa,
            Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Nunoa,
            Santiago, RM 780000, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: unicycler v. 0.4.9b; Canu v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 204X
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030061665.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 06:01:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,265
            CDSs (with protein)               :: 5,265
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 53 of 130
            Pseudo Genes (incomplete)         :: 81 of 130
            Pseudo Genes (internal stop)      :: 17 of 130
            Pseudo Genes (multiple problems)  :: 20 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093470.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="VA681"
                     /isolation_source="Isolated from an abscess of an 32 years
                     old female"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pVA681-5"
                     /geo_loc_name="Chile: Metropolitan Region"
                     /collection_date="2019-04-24"
                     /collected_by="Instituto de Salud Pblica de Chile"
     gene            join(4286..4546,1..237)
                     /locus_tag="MO412_RS27495"
                     /old_locus_tag="MO412_27465"
     CDS             join(4286..4546,1..237)
                     /locus_tag="MO412_RS27495"
                     /old_locus_tag="MO412_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            244..459
                     /locus_tag="MO412_RS27500"
                     /old_locus_tag="MO412_27470"
     CDS             244..459
                     /locus_tag="MO412_RS27500"
                     /old_locus_tag="MO412_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(878..1084)
                     /locus_tag="MO412_RS27595"
     CDS             complement(878..1084)
                     /locus_tag="MO412_RS27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_349291579.1"
                     /translation="MGEISVKTGNNARNGGKNSLNRLIRCVCGKKIGPDPRNFNQRVSL
                     GRNDLLIRTFPSHSLHPRYIKST"
                     /name="hypothetical protein"
     gene            complement(1066..2046)
                     /locus_tag="MO412_RS27505"
                     /old_locus_tag="MO412_27475"
     CDS             complement(1066..2046)
                     /locus_tag="MO412_RS27505"
                     /old_locus_tag="MO412_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_000019473.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            complement(2504..2635)
                     /locus_tag="MO412_RS27510"
                     /old_locus_tag="MO412_27480"
     CDS             complement(2504..2635)
                     /locus_tag="MO412_RS27510"
                     /old_locus_tag="MO412_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            3267..3608
                     /locus_tag="MO412_RS27515"
                     /old_locus_tag="MO412_27485"
     CDS             3267..3608
                     /locus_tag="MO412_RS27515"
                     /old_locus_tag="MO412_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3817..>4245
                     /locus_tag="MO412_RS27520"
                     /old_locus_tag="MO412_27490"
                     /pseudo=""
     CDS             3817..>4245
                     /locus_tag="MO412_RS27520"
                     /old_locus_tag="MO412_27490"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3008..3183
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.449"
                     /coverage="81.0"
                     /evalue="5.74e-59"
     Typing          complement(2527..3025)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.04"
                     /coverage="92.0"
                     /evalue="5.84e-177"
     Typing          4..575
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.476"
                     /coverage="99.0"
                     /evalue="2.83e-112"
     Typing          3599..4545
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgttcgcca gcctgagcgg ggtgctgtgg taccagggga accggatagc ggcgaacctg
       61 gcggaaatca ggcagcagag agatacgctg tcgaaactgc acatgcagac gtggggcgtg
      121 acctatctgg agaaccgcaa cggacggttt ctggtgctgc cggaggggat gaaagccgaa
      181 acgggccgga cggtggacaa caagacgcgg aacgcggtga agctggtacg ggagtagagc
      241 agaatgacgg aactggaaaa acaattgttg agcgcgttag agcagctaca gcaggactac
      301 tcgagaaggc tggacgagtg ggagagcgcc ttcgaggaat ggcggaagat gtctggtctt
      361 atgcaacggg agaacgcggc gctgagcgag cgcgtgtcgg gcttgagcag gcaggtgcag
      421 agtttgagcg agcagctgcg ccggttatcg acaggctgaa tgagattgag caacagcgat
      481 acgggcaaac aacggaaaag gaaccagaga agacagcaga tctggtaccg gaacggcaac
      541 gcacattatc tcatggtgac agggagtatg gagactgata aacgcccgtc tcaggtggcc
      601 tgagcgtgac ggggatcatg gccattttct gcgatttcag cacacagaga gtctttttta
      661 accgaaatca ccggctatgc agtgatgtga ataagtgatg catatgcgta aaataaatca
      721 ggtgcgtttc tggtggtttt cggtgtactt gccaccacct tttgcccgtc agccatcaga
      781 aacgccctga gtgcctccgg cgcggtgcgg gtaagagatg ttatgttcaa tcgaaatcag
      841 aaaaacgatc ggtaaagcga tcgtttttct gatagcatca tgttgatttt atataacgtg
      901 ggtgtaaaga atgactaggg aaggtgcgaa taagcaggtc atttcttccc aagctgactc
      961 gctgattaaa atttcgcgga tctgggccga tttttttccc gcaaacacat cgaatcagcc
     1021 tatttaggct attttttcca ccatttctgg cgttatttcc ggtttttact gagatctctc
     1081 ccactgacgt atcatttggt ccacccgaaa caggttggcc agggtgaata acatcgccag
     1141 ttggttatcg tttttcagca gccctttgta tctggctttc acgaagccga actgccgctt
     1201 gatgatgcga aacgggtgct ccaccctggc acggatgctg gctttcatgt attcgatgtt
     1261 gatggccgtt ttgttcttgc gcggatgctg cttcaaggtt tttaccttgc cgggacgctc
     1321 ggcgatcagc cagtccacat ccacctcggc cagctcctcg cgctgtggcg ctccttggta
     1381 gccggcatcg gctgagacaa attgctcctc tccatgaagc agattaccca gctgattgag
     1441 gtcatgctcg ttggccgcgg tggtgactag gctgtgggtc aggccactct tggcatcgac
     1501 accaatgtgg gccttcatgc caaagtgcca ctgattgcct ttcttggtct gatgcatctc
     1561 cggatcgcgt tgctgctctt tgttcttggt agagctgggt gcctcaatga tggtggcatc
     1621 caccaaagtg ccttgggtca tcatgacgcc tgcttcggcc agccagcgat tgatggtctt
     1681 gaacaattga cgggccagtt gatgctgctc gagcaggtgg cggaaattca tgatggtggt
     1741 gcgatccggc agggcgctat ccagggataa tcgggcaaac aggcgcatgg aggcgatttc
     1801 gtacagggca tcttccatgg caccgtcgct caggttgtac caatgctgca tgcagtgaat
     1861 acgcagcatg gtctccagcg gatagggccg tcggccattg cccgccttgg gataaaacgg
     1921 ctcgatgact tccaccatgt tttgccatgg cagaatctgc tccatgcggg agaggaaaat
     1981 ctcttttcgg gtctgacggc gcttagtgct gaattcacta tcggcgaagg tgagttgatg
     2041 gctcatgatg tccctctggg atgcgctccg gatgaatatg atgatctcat atcaggaact
     2101 tgttcgcacc ttccttagat gtagagctat ttccgctcag tttgtttcga gcgatttgat
     2161 tgcactgttt gagctaaaga tagacgaagg acaagtgaaa ttgattgagg aaaggcatta
     2221 caaactggtt ccggtaaaag agttagatag ctctgacatc tcaaactacc gcatgtctgt
     2281 ataatttctg agaggatctt cttgagatcc tttcgttcta gccgtaatct cctaagccgt
     2341 aaacaaaaaa accaccgcta ccaacggtgg ttcgtttgcc ggaatttaga gctaccaact
     2401 ctttcttccg aaggtaactg gctttcacgg agcgcagata ccaaaactgt cctttcagtg
     2461 tagccgtagt caggccacca cttcaagaac tctgtagata actctactcc gctccgtgaa
     2521 tccgattacc agtggctgct gccagtggcg cttttgcatg tcttttcggg ttggactcaa
     2581 gacgatagtt accggacaag gcgcagcagt cggactgaac ggggggttcg tgcatacagt
     2641 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc
     2701 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     2761 gagctaccag ggggaaacgc ctgggatctt tatagtcctg tcgggtttcg ccaccactga
     2821 tttgagcgtc agattttgtg atgcttgtca ggggggcgga gcctatggaa aaacggcaac
     2881 gccgcatcct ttttcctgac tttcctgact ttcctgactt tcctgacttg agcgcgatct
     2941 cgttatctcg tccttcgtga gctggtaacg cccgccgcag tctcacggca ggagcgcagc
     3001 gaccgagtga gcgaggaggc gtcatttcat cgttacctgc attttgcact tacgccccgc
     3061 agccgtattt tcccccttgc cacatgaagc acttccccga atctccatct cgccgtaaca
     3121 tactaagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg cgccccgaaa
     3181 ccccactgac gcgcctgcgg cttgtccaac cccgcgccga ccgggaaagg ctttcccgtc
     3241 cgtcacggcg ttatcaggag gcgattctgg cagataaacg caacaagatg ctcacgatgt
     3301 gggtgacgga agacgaacac cggcggttgc ttgagcggtg cgacggaaaa caactggcgg
     3361 cgtggatgcg ccagacctgc cttgatgaga aaccgacacg cgccggcagg cttccctcga
     3421 tttcgcccgc gctgctgcgt cagcttgccg gcatgggcaa taacctgaac cagatcgccc
     3481 ggaaagtgaa cgccggtggt gccggtcatg accgggtgca gatcgtggca gcgctgatgg
     3541 cgatcgatgc cggtctggaa cggttgcggc atgccgtact ggaaaaaggg ccggacgatg
     3601 attgttaaat tccatccccg gggtcgcggc gggggcgccg gtcctgtgga ttatcttctg
     3661 ggtaaagacc ggcagcgtga aggtgccagc gtcctgcagg ggaagccgga agaagtccgg
     3721 gagctgattg atgcgtcgcc gtatgtgaaa aaatatacct ccggggtgct gtcattcgcg
     3781 gaggcggacc tgccgccggg tcagcgtgaa aaactgatgg ccagctttga gcgggttctg
     3841 atgcccggac tcgataaaga ccagtacagc attctgtggg tggaacacgc agacaagggt
     3901 cgtcttgagc tgaattttct gatccccaat accgaattac tgacggggaa acgcctccag
     3961 ccgtattacg atcgtgccga ccggccgcgc atcgatgcgt ggcagacagt ggtcaacggc
     4021 aggctggggc tacatgatcc gaacgacccg gagaaccggc gggcgctggt cacgccttca
     4081 ggactgccag aaacgaagca ggaagccgca gagacgatta cacggggttt gctgatgtta
     4141 gcctcatcag gagagctgaa aacgcgtgat gacgtcacag gggcgctcac agcggccggt
     4201 tttgaggtgg tcagaaccac aaaaaacagt atcagcattg ccgacccgga cggggggcga
     4261 aatatccgac tgaggggagc catttatgag cagtctttta acggcctcga gggacttcga
     4321 ggagaaatcg aaagcacagc agcagcttac cgaagagagg ctgaaagccg cattcagcaa
     4381 gcacgagaac gttgtcaaat cggaactgaa cgcaagcgcg aagagaatca acgacgccat
     4441 cagcgcccac gaacagggga tgaacgcggc gatgcagtcg aaccggctga gcgtgctgcg
     4501 aatggtcggg cggacgtggc tgaccatcac catggtgtca gggctg
//
