LOCUS       NZ_CP093462             8444 bp    DNA     circular CON 11-JUN-2024
DEFINITION  Klebsiella pneumoniae strain VA684 plasmid pVA684-8, complete
            sequence.
ACCESSION   NZ_CP093462
VERSION     NZ_CP093462.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26642722
            Assembly: GCF_030061645.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Complete genome sequencing and analysis of carbapenem-resistant
            Klebsiella pneumoniae isolated in Chile
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Marcoleta,A.E., Hormazabal,J.C. and Araya,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2022) Biology, Grupo de Microbiologia Integrativa,
            Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425 Nunoa,
            Santiago, RM 780000, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: unicycler v. 0.4.9b; Canu v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 195X
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030061645.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 06:17:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,516
            Genes (coding)                    :: 5,352
            CDSs (with protein)               :: 5,352
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 63 of 164
            Pseudo Genes (incomplete)         :: 96 of 164
            Pseudo Genes (internal stop)      :: 36 of 164
            Pseudo Genes (multiple problems)  :: 28 of 164
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093462.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="VA684"
                     /isolation_source="Isolated from catheter blood of an 15
                     years old female"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pVA684-8"
                     /geo_loc_name="Chile: Metropolitan Region"
                     /collection_date="2019-04-25"
                     /collected_by="Instituto de Salud Pblica de Chile"
     gene            complement(join(7822..8444,1..72))
                     /locus_tag="MO416_RS28045"
                     /old_locus_tag="MO416_28010"
                     /pseudo=""
     CDS             complement(join(7822..8444,1..72))
                     /locus_tag="MO416_RS28045"
                     /old_locus_tag="MO416_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(<136..1104)
                     /locus_tag="MO416_RS28050"
                     /old_locus_tag="MO416_28015"
                     /pseudo=""
     CDS             complement(<136..1104)
                     /locus_tag="MO416_RS28050"
                     /old_locus_tag="MO416_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RnlA family toxin"
                     /name="type II toxin-antitoxin system RnlA family toxin"
     gene            complement(1340..1486)
                     /locus_tag="MO416_RS28240"
     CDS             complement(1340..1486)
                     /locus_tag="MO416_RS28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_349307638.1"
                     /translation="MYARTPVQSDRCALSGNYHLESNPERHDKAPLAAATGNGFAEQRS
                     VEV"
                     /name="replication initiation protein"
     gene            2043..2273
                     /locus_tag="MO416_RS28055"
                     /old_locus_tag="MO416_28020"
     CDS             2043..2273
                     /locus_tag="MO416_RS28055"
                     /old_locus_tag="MO416_28020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_283447406.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHARWILGRLSA
                     GKRYSFFAYPRTCNTPVLHLISLFSCDYRWL"
                     /name="hypothetical protein"
     gene            3023..3250
                     /locus_tag="MO416_RS28060"
                     /old_locus_tag="MO416_28025"
     CDS             3023..3250
                     /locus_tag="MO416_RS28060"
                     /old_locus_tag="MO416_28025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_283447407.1"
                     /translation="MAKVTRIQGGQYAVDDGRFIVKNSSGWLVVDANGSHDFGPVPTLT
                     AAKQYVESGTTPLGQHNFPPITVADRAKRV"
                     /name="hypothetical protein"
     gene            complement(3489..3866)
                     /locus_tag="MO416_RS28065"
                     /old_locus_tag="MO416_28030"
                     /pseudo=""
     CDS             complement(3489..3866)
                     /locus_tag="MO416_RS28065"
                     /old_locus_tag="MO416_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RnlB family
                     antitoxin"
                     /name="type II toxin-antitoxin system RnlB family
                     antitoxin"
     gene            complement(3951..4647)
                     /locus_tag="MO416_RS28070"
                     /old_locus_tag="MO416_28035"
     CDS             complement(join(3951..4395,4394..4647))
                     /locus_tag="MO416_RS28070"
                     /old_locus_tag="MO416_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X2 family transposase"
                     /protein_id="WP_283447408.1"
                     /translation="MASISISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHLKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1X2 family transposase"
     gene            complement(<4711..>4887)
                     /locus_tag="MO416_RS28075"
                     /old_locus_tag="MO416_28040"
                     /pseudo=""
     CDS             complement(<4711..>4887)
                     /locus_tag="MO416_RS28075"
                     /old_locus_tag="MO416_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA endoribonuclease LsoA"
                     /name="mRNA endoribonuclease LsoA"
     gene            complement(5211..5357)
                     /locus_tag="MO416_RS28245"
     CDS             complement(5211..5357)
                     /locus_tag="MO416_RS28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_349307638.1"
                     /translation="MYARTPVQSDRCALSGNYHLESNPERHDKAPLAAATGNGFAEQRS
                     VEV"
                     /name="replication initiation protein"
     gene            5912..6061
                     /locus_tag="MO416_RS28080"
                     /old_locus_tag="MO416_28045"
     CDS             5912..6061
                     /locus_tag="MO416_RS28080"
                     /old_locus_tag="MO416_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001442891.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            6893..7195
                     /locus_tag="MO416_RS28085"
                     /old_locus_tag="MO416_28050"
     CDS             6893..7195
                     /locus_tag="MO416_RS28085"
                     /old_locus_tag="MO416_28050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_283447404.1"
                     /translation="MAKVTRIQGGQYAVDDGRFIVKNSSGWLVVDANGSHDFGPVPTLT
                     AAKQYVESGTTPLGQHNFSTNYGRRQSKKSLMHILPLKLKMETRCQPSFGSLFFA"
                     /name="hypothetical protein"
     gene            complement(7360..7737)
                     /locus_tag="MO416_RS28090"
                     /old_locus_tag="MO416_28055"
                     /pseudo=""
     CDS             complement(7360..7737)
                     /locus_tag="MO416_RS28090"
                     /old_locus_tag="MO416_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RnlB family
                     antitoxin"
                     /name="type II toxin-antitoxin system RnlB family
                     antitoxin"
     Typing          1835..2050
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.636"
                     /coverage="100.0"
                     /evalue="7.33e-68"
     Typing          5705..5919
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.636"
                     /coverage="100.0"
                     /evalue="7.33e-68"
     Typing          complement(1371..1885)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.333"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5242..5755)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.143"
                     /coverage="99.0"
                     /evalue="3.49e-179"
ORIGIN
        1 ggtgcttttg ccgttacgca ccaccccgtc agtagctgag caggagggac aactgatgga
       61 gatggaagcc acgggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcac
      121 cattaccgat tctgctttgt caattgtctt agtgtcagcc acacctggtt tcatatggaa
      181 caagccatgt ctgttttcat gccagtaagt gtagcactca cacaaaactt ctgctatttc
      241 accattaata gtttcttggt gtatatctct tattatgtat ttcgggttat tctcagtgaa
      301 gtactcgcca agattccttg atgatgatgg gttgcatatg cttccaagta gttgatatat
      361 aaagccttct atggatctca aggctgagaa acaatatgtt gagtaatctt ccatttcgac
      421 atctatttgt ttcattatga gcgagcagga tagctgtttt ttgatgtcgt catggattag
      481 gtcgatgctt tttggtagtt tactatgtaa ttctgactca attgtttctt tttttatgtc
      541 aacattaaat tcttgattct tctgttctat aatctctttc atgtccagta tggtgcacat
      601 gaaatcatta atcaaacttg cgatttgaag atatttacct tggaattgaa ttgagccacg
      661 gttgtaaatt tttgctctga ctctgtcacc attgccagtg gtcatgatgt attgatgttt
      721 acagttgggg tcgttgtttt tatcttcttc tatttttacg ttttcttcag taagaaatgc
      781 aaacaacatt tccttatttt catcgctgaa tcttgggatt gtgtattcaa acgttttttt
      841 atctgaatac aaacagtttt cttttataat gccagcgatc tcatcaaagt aagtgttgtt
      901 ttgtcctgat gcccttccta tagtggtgtt tccacctgat ggcattagct ttattaaaaa
      961 atctatttct ttatatgtcc ctgataaatg tgtttctctt tcgtttctca cgatagaaga
     1021 aaagtttgtg acgccattct gcgtcaaggc agcttcaatc ttgtctgtat taatatttag
     1081 tgctttaaat gggttctgtg ccattgcctc aatccgttgt tttttctgaa tatgtacaga
     1141 tcttgttttt tttgtcaatg gaagcgcggt tcgttgactt gatagactga ttgattcatc
     1201 atcgcataaa gaaaaaacca ccgctaccaa cggtggtttt ctcaaggttc gttgagctac
     1261 caactctttg aaccgaggta actggctttc gcagagcaca gaaaccaaat tcgttattac
     1321 agtgtagctg tacttcacct cacacttcaa cacttctttg ttctgcgaat ccgttaccag
     1381 tggctgctgc cagtggcgct ttgtcgtgcc tttccgggtt ggactcaaga tgatagttac
     1441 cggacaaggc gcagcggtcg gactgaacgg gggttcgtgc atacagtcca gcttggagcg
     1501 aactgcctac ccggaactga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg
     1561 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccatcagggg
     1621 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     1681 ttcgtgatgc ttgtcagggg gcggagccta tggaaaaacg gcagcgccac atcctttttc
     1741 cggcctttcc tgacttgagt gccatctctt tatcttgtcg tttgtgagca ggtaacgccc
     1801 gccgcagcct cacggcagga gcgtagcgac cgagtgagcg aggaggcgtc atatcaccgt
     1861 aactcccatt ttctccctta cgccctgtag ctgcattttc ccctgtcaca tgaagcactt
     1921 tcattaggtg ctactccgcc gtaacatatt cagccactgt acactccgct agcgctacgt
     1981 gactggttca gggctgcgct ccgaaacccg ctaacaacct ctccgctact ggatatcacc
     2041 ccatgccatc tgaatatgat gaacaactgg attcgactga tacgcagctt cccggcgaat
     2101 atgacctttt tgatttaatg gcgcaggtta ctgaagagaa cctgcacgcc cggtggattt
     2161 tgggccgcct gtcggccggg aagcgttata gctttttcgc ttacccgcgt acgtgtaata
     2221 cgccggtatt acatcttatt tctttatttt catgtgatta caggtggttg taatacgttg
     2281 cgtattacat aaataaacgg gaacttcagt ttttaccttt taattcatgc aattgcgatg
     2341 aaaatcgcgg ttgcgtatgg tgtatggcca tttaagggat aaagaatcat ctccttaccg
     2401 ggctgagctt tgcctattct gaggagatct gtgaaaaaag cgtccataac tagctcgttc
     2461 atacttgtat gaacagctta ttcctcctga ctactaataa actacagcgt cactactgat
     2521 ttactactga tttactacaa accggagggg actacagaaa cactactgat ttactactga
     2581 tttactacaa atcggaggag gatgactact tatctactac tgatttacta ctgaattact
     2641 actgaattac gtcagaacag ttctctgaac cattcgtaca tccgaccccc ttatcttcat
     2701 gcttgatgtt cttgccaacg cctcacggcc acgcaataaa ttgcgtcccc gttcaccgtt
     2761 ttcgggtggg aagaacgccg gaaacaggac ttaccccggc tgaatggcgt gaatttccgc
     2821 actatgcact tgcgcgcata ctcatgcatg ccgtaaaaac agagcctgcg cgtttctggc
     2881 gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg ccctgagagc
     2941 ctctcaggcg gtgcacgcaa cagatgttat ggtaaataca atgagctgct gaaagcggcc
     3001 tgatagacat taggagaatg tattggcgaa ggtcactaga atccagggtg gacaatatgc
     3061 agtggatgat gggcgtttta ttgtaaaaaa tagctcaggc tggcttgtgg ttgatgccaa
     3121 tggttctcat gattttggac ctgttcctac gctaaccgct gcgaagcaat atgttgagtc
     3181 tggaaccact ccgttaggac agcataattt tccaccaatt acggtcgcag acagagcaaa
     3241 aagagtttaa tgcatatctt gcctctgaag ctaaaaatgg aaacccgctg ccagccatcc
     3301 tttggttcat tgttctttgc ataatctgtg gtgttgtatt ggcagttcga ggttattagg
     3361 taaggttgat atgcatatcc tttggtctct atggactatc gacgtgaagc ctacgactga
     3421 agcccgcaaa gcgggctttt tcatgcccgg cggacaacgg atgttatgtt caatatctga
     3481 acttgctttt atattggaat gccttgtttt aaagcaaatg atgtggcttt agatagaagg
     3541 tttgcattaa tattattaaa gttattctta aagaacgaag ctgagcgttc cgataaggat
     3601 ttatacttat ctgtttccgt aactttgttt aatgtgtagc ttgtcaatcg ctcgccgttg
     3661 aatgatgtat ggacgtaacg gtcttttttc caccattagg aattattaaa tcaaaaaaac
     3721 atcgccacca actttctttt tctttaaatc agactcgatt ttagagaaaa ttcgctcggg
     3781 ctataaatat ctgtagcgta gacaatgaat aaagttttat ctttatcctt tatcacctct
     3841 atttgatatt ttttgtttat tttcattatt tcaacctagc catgccttta tctatcaact
     3901 gacatacagt atcgataatt tcaatagatt ctgctggtaa tgactccaac ttactgatag
     3961 tgttttatgt tcagataatg cccgatgacc ttgtcatgca gctccaccga ttttgagaac
     4021 gacagtgact tccgtcccag ccttgccaga tgttgtctca gattcaggtt atgtcgctca
     4081 atgcgctgag tgtaacgctt gctgattacg tgcagctttc ccttcaggcg ggattcatac
     4141 agcggccagc catccgtcat ccataccacg acctcaaagg ccgacagcag gctcagaaga
     4201 cgctccagtg tggccagagt gcgttcaccg aagacgtgcg ccacaaccgt cctccgtatc
     4261 ctgtcatacg cgtaaaacag ccagcgctga cgtgatttag caccgacgta gccccactgt
     4321 tcgtccattt cagcgcagac aatcacatca ctgcccggct gtatgcgcga ggttaccgac
     4381 tgcggcctga gtttttaagt gacgtaaaac cgtgttgagg ccaacgccca taatgcgtgc
     4441 actggcgcga catccgacgc cattcatggc catatcaatg attttctggt gcgtaccggg
     4501 ctgagaggcg gtgtaagtga actgtagttg ccatgtttta cggcagtgag agcagagata
     4561 gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgagcagga
     4621 gggacaactg atggagatgg aagccacggg agcacctcaa aaacaccatc atacactaaa
     4681 tcagtaagtt ggcaccatta ccgattctgc tttgtcaatt gtcttagtgt cagccacacc
     4741 tggtttcata tggaacaagc catgtctgtt ttcatgccag taagtgtagc actcacacaa
     4801 aacttctgct atttcaccat taatagtttc ttggtgtata tctcttatta tgtatttcgg
     4861 gttattctca gtgaagtact cgccaagaga agaaaagttt gtgacgccat tctgcgtcaa
     4921 ggcagcttca atcttgtctg tattaatatt tagtgcttta aatgggttct gtgccattgc
     4981 ctcaatccgt tgttttttct gaatatgtac agatcttgtt ttttgtcaat ggaagcgcgg
     5041 ttcgttgact tgatagactg attgattcat catcgcataa agaaaaaacc accgctacca
     5101 acggtggttt tctcaaggtt cgttgagcta ccaactcttt gaaccgaggt aactggcttt
     5161 cgcagagcac agaaaccaaa ttcgttatta cagtgtagct gtacttcacc tcacacttca
     5221 acacttcttt gttctgcgaa tccgttacca gtggctgctg ccagtggcgc tttgtcgtgc
     5281 ctttccgggt tggactcaag atgatagtta ccggacaagg cgcagcggtc ggactgaacg
     5341 ggggttcgtg catacagtcc agcttggagc gaactgccta ccggaactga gtgtcaggcg
     5401 tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     5461 caggagagcg cacgagggag ccatcagggg aaacgcctgg tatctttata gtcctgtcgg
     5521 gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg gcggagccta
     5581 tggaaaaacg gcagcgccac atcctttttc cggcctttcc tgacttgagt gccatctctt
     5641 tatcttgtcg tttgtgagca ggtaacgccc gccgcagcct cacggcagga gcgtagcgac
     5701 cgagtgagcg aggaggcgtc atatcaccgt aactcccatt ttctccctta cgccctgtag
     5761 ctgcattttc cctgtcacat gaagcacttt cattaggtgc tactccgccg taacatattc
     5821 agccactgta cactccgcta gcgctacgtg actggttcag ggctgcgctc cgaaacccgc
     5881 taacaacctc tccgctactg gatatcaccc catgccatct gaatatgatg aacaactgga
     5941 ttcgactgat acgcagcttc ccggcgaata tgaccttttt gatttaatgg cgcaggttac
     6001 tgaagagaac ctgcacgccc cggtggattt tgggccgcct gtcggccggg aagcgttata
     6061 gctttttcgc ttacccgcgt acgtgtaata cgccggtatt acatcttatt tctttatttt
     6121 catgtgatta caggtggttg taatacgttg cgtattacat aaataaacgg gaacttcagt
     6181 ttttaccttt taattcatgc aattgcgatg aaaatcgcgg ttgcgtatgg tgtatggcca
     6241 tttaagggat aaagaatcat ctccttaccg ggctgagctt tgcctattct gaggagatct
     6301 gtgaaaaaag cgtccataac tagctcgttc atacttgtat gaacagctta ttcctcctga
     6361 ctactaataa actacagcgt cactactgat ttactactga tttactacaa accggagggg
     6421 actacagaaa cactactgat ttactactga tttactacaa atcggaggag gatgactact
     6481 tatctactac tgatttacta ctgaattact actgaattac gtcagaacag ttctctgaac
     6541 cattcgtaca tccgaccccc ttatcttcat gcttgatgtt cttgccaacg cctcacggcc
     6601 acgcaataaa ttgcgtcccc gttcaccgtt ttcgggtggg aagaacgccg gaaacaggac
     6661 ttaccccggc tgaatggcgt gaatttccgc actatgcact tgcgcgcata ctcatgcatg
     6721 ccgtaaaaac agagcctgcg cgtttctggc gggttttcgg gtggtttgtt gcctgtttta
     6781 ccggtttccc gtcagaaacg ccctgagagc ctctcaggcg gtgcacgcaa cagatgttat
     6841 ggtaaataca atgagctgct gaaagcggcc tgatagacat taggagaatg tattggcgaa
     6901 ggtcactaga atccagggtg gacaatatgc agtggatgat gggcgtttta ttgtaaaaaa
     6961 tagctcaggc tggcttgtgg ttgatgccaa tggttctcat gattttggac ctgttcctac
     7021 gctaaccgct gcgaagcaat atgttgagtc tggaaccact ccgttaggac agcataattt
     7081 ttccaccaat tacggtcgca gacagagcaa aaagagttta atgcatatct tgcctctgaa
     7141 gctaaaaatg gaaacccgct gccagccatc ctttggttca ttgttctttg cataatctgt
     7201 ggtgttgtat tggcagttcg aggttattag gtaaggttga tatgcatatc ctttggtctc
     7261 tatggactat cgacgtgaag cctacgactg aagcccgcaa agcgggcttt ttcatgcccg
     7321 gcggacaacg gatgttatgt tcaatatctg aacttgcttt tatattggaa tgccttgttt
     7381 taaagcaaat gatgtggctt tagatagaag gtttgcatta atattattaa agttattctt
     7441 aaagaacgaa gctgagcgtt ccgataagga tttatactta tctgtttccg taactttgtt
     7501 taatgtgtag cttgtcaatc gctcgccgtt gaatgatgta tggacgtaac ggtctttttt
     7561 ccaccattag gaattattaa atcaaaaaaa catcgccacc aactttcttt ttctttaaat
     7621 cagactcgat tttagagaaa attcgctcgg gctataaata tctgtagcgt agacaatgaa
     7681 taaagtttta tctttatcct ttatcacctc tatttgatat tttttgttta ttttcattat
     7741 ttcaacctag ccatgccttt atctatcaac tgacatacag tatcgataat ttcaatagat
     7801 tctgctggta atgactccaa cttactgata gtgttttatg ttcagataat gcccgatgac
     7861 cttgtcatgc agctccaccg attttgagaa cgacagtgac ttccgtccca gccttgccag
     7921 atgttgtctc agattcaggt tatgtcgctc aatgcgctga gtgtaacgct tgctgattac
     7981 gtgcagcttt cccttcaggc gggattcata cagcggccag ccatccgtca tccataccac
     8041 gacctcaaag gccgacagca ggctcagaag acgctccagt gtggccagag tgcgttcacc
     8101 gaagacgtgc gccacaaccg tcctccgtat cctgtcatac gcgtaaaaca gccagcgctg
     8161 acgtgattta gcaccgacgt agccccactg ttcgtccatt tcagcgcaga caatcacatc
     8221 actgcccggc tgtatgcgcg aggttaccga ctgcggcctg agtttttaag tgacgtaaaa
     8281 ccgtgttgag gccaacgccc ataatgcgtg cactggcgcg acatccgacg ccattcatgg
     8341 ccatatcaat gattttctgg tgcgtaccgg gctgagaggc ggtgtaagtg aactgtagtt
     8401 gccatgtttt acggcagtga gagcagatag cgctgatgtc cggc
//
