LOCUS       NZ_CP093288             2640 bp    DNA     circular CON 25-MAY-2024
DEFINITION  Escherichia coli strain EC-14-2-9 plasmid pEC-14-2-9-6, complete
            sequence.
ACCESSION   NZ_CP093288
VERSION     NZ_CP093288.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26549971
            Assembly: GCF_022605345.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Qin,S. and Deng,R.
  TITLE     EC-14-2-9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Qin,S. and Deng,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2022) Institute of Drug Discovery & Development,
            ZhengZhou University, 100 Science Avenue, ZhengZhou, HeNan 450001,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022605345.1-RS_2024_05_25
            Annotation Date                   :: 05/25/2024 05:14:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,082
            Genes (coding)                    :: 4,822
            CDSs (with protein)               :: 4,822
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 90 of 260
            Pseudo Genes (incomplete)         :: 175 of 260
            Pseudo Genes (internal stop)      :: 58 of 260
            Pseudo Genes (multiple problems)  :: 57 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093288.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC-14-2-9"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC-14-2-9-6"
                     /geo_loc_name="China: Zhengzhou"
                     /lat_lon="34.76 N 113.65 E"
                     /collection_date="2014-12-19"
                     /collected_by="Zhengzhou University"
     gene            complement(116..445)
                     /locus_tag="MNY25_RS25835"
                     /old_locus_tag="MNY25_25835"
     CDS             complement(116..445)
                     /locus_tag="MNY25_RS25835"
                     /old_locus_tag="MNY25_25835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052909321.1"
                     /translation="MKILPAEPGLYFFASFESGAHVYKVVAWSENENGGLDPQCVLTAL
                     YDKSSSSQQWELHSTSASAYQHSNDGRPFMYFITYFTSNGGIPELYNAINTLAERWGYP
                     LIDLP"
                     /name="hypothetical protein"
     gene            complement(529..960)
                     /locus_tag="MNY25_RS25840"
                     /old_locus_tag="MNY25_25840"
     CDS             complement(529..960)
                     /locus_tag="MNY25_RS25840"
                     /old_locus_tag="MNY25_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_001025244.1"
                     /translation="MNIPTHLKHKPIIAVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     YTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSRSG
                     GNPELSRHIDLMKNELKENHEHLDESLKRLAAELKRLGY"
                     /name="DUF6530 family protein"
     gene            1980..2180
                     /locus_tag="MNY25_RS25845"
                     /old_locus_tag="MNY25_25845"
     CDS             1980..2180
                     /locus_tag="MNY25_RS25845"
                     /old_locus_tag="MNY25_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_023139298.1"
                     /translation="MARSPDPRQRTEGFRKAEASLRLEGMDPAGTPLYENIKERVISGD
                     ITYEQGRAEILAHYQKRANGH"
                     /name="antitoxin VbhA family protein"
     Typing          complement(1278..1783)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.932"
                     /coverage="96.0"
                     /evalue="9.94e-175"
ORIGIN
        1 gcgcagaaat catcaggcgc gttttttcac gatatggaca gggaaagatg ccggaaatag
       61 gcaggagggc gcagaaatgc cctgagggcg tctgagatgg tgcacgcaac gaatgttatg
      121 gtaaatcaat taatgggtat ccccatcgtt cggctagtgt gtttattgcg ttataaagtt
      181 caggtatccc gccattgctg gtgaagtagg tgataaaata cataaatggt ctgccgtcat
      241 tgctgtgctg atatgcagat gctgaagttg agtgaagttc ccattgctgg ctggaactgc
      301 tcttgtcata taaggccgtt aacacacact gtggatctaa accaccattc tcattttctg
      361 accaggccac tactttgtaa acgtgagcac cggactcaaa agatgcgaaa aaatataatc
      421 ccggttcagc tggtaggatc ttcattagct agcttccttt tgtttattct gtctgagagg
      481 caataactac ctaattcccc gtggtgggta taaaaaatca cggggaggtt aataacctaa
      541 acgttttagt tctgctgcta aacgcttaag actctcatct aaatgctcgt gattctcttt
      601 tagctcgttt ttcattaaat caatgtgacg agataactca ggattgcctc ctgaacgact
      661 gaccggaaag tcgtcgctgg aagataagcg gccatgttta ctgtaatcca ttgctatgca
      721 gataagcgtt gccaagtcga taacacgatg aagaggtaat tcttctgatt gccgtgacca
      781 cttttctccg gtgttgcgcc acacttttgc tgatagctcg gtatacccgg gggtgttcca
      841 ttgagcaatt ccaacggaaa gccccatagc atcagtgtca tcagcataag gtccatcaat
      901 tcgatcatag ttttctacag cgataatcgg tttgtgtttc agatgagtag ggatgttcat
      961 atcattctct tttatgtgaa tttaggttgt taattaacaa aataacaaaa cagcttattt
     1021 tactaattag taagtgtaac attttgtgaa ttgatggatt tgaagttgtt ggcgtggatc
     1081 ttcttgagat catttatggc agagcgtaat ctcttgctca gaaaacgaaa aaaccaccct
     1141 ggcaggtggt ttttcgaagg ttagttaatc ctggcagatt ctctaaccgt ggtaacagtc
     1201 ttgtgcgaga catgtcacca aatctgtcct ttcagtgtag ccgcagtcag gccaccactt
     1261 caagaactct cgatacatct ctcgcacatc ctgtttacca gtggctgctg ccagtggcgc
     1321 tttgtcgtgt ctttccgggt tggactcaag ttgatagtta ccggaaaagg cgcagcagtc
     1381 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct tcccggaatc
     1441 gagtgtcagg cgtggaatga gataaacgcg gccataacag cggaatgaca ccggtaaacc
     1501 gaatggcagg aacaggagag cgcacgaggg agctaccagg gggaaacgcc tgggatcttt
     1561 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gattctgtga tgcttgtcag
     1621 gggggcgaag cctatggaaa aacggcttcg ccgcggcctt ttctgttccc ctctttctcc
     1681 ttccgctacc cccgaatctg tgggtaactc caggtggact gccggtaccg ctcgccgcag
     1741 ccgaacggcc gagcgtagcg agtcagtgag cgaggaagcg gaagatctgc gatgcctgat
     1801 tgggctcgtg tcgcgccgta ccctctcttt accgccggat tacacagttt cctgcaatac
     1861 tttccttccg gtttacacat tcgtctgcac ggataaacaa cggcagagta cgggcaggat
     1921 atgtaaagta ggcctacttt accgcctgcc ttattcgctt acagctcaaa ggaacatcga
     1981 tggccaggtc ccccgatcca cgacaacgta ctgagggttt ccgcaaagcg gaagcgtcgc
     2041 tgaggcttga aggcatggac cccgccggaa cgccgctgta cgaaaatatc aaggagcggg
     2101 ttatttccgg ggatatcact tacgaacagg gacgggcaga aattctggct cattaccaga
     2161 agcgtgcgaa cggccactga cacggacagg aaactaggta ccatcgcttc ctcaaaaaaa
     2221 caggcgtggc ctctctccgg ccaggctttt cctgtatttg ctatctgttt gctacttgtt
     2281 tgctatcttt ttgctatctg tttgctactt gtttgctatc tgtttgctac ttgtttgcta
     2341 ccgctaccct ctgctgtgaa aagagtaagg cacgttagcg atgtatacat cgccgcgccg
     2401 caagggttcc ccttccgaat cccgttcatc actgcgtgct gattatccac gttaaatccg
     2461 acctcctgct tggtagtaaa ttctgccatg cctgacggcc tggccaacgc ctcacggtcg
     2521 cacagtaaac tgtgcacccg ttcaccgtta tcgggggagt tatgcgccgg aaagcaggct
     2581 ttcatgccag catccacccc aaatcgaccg ccattcacct gcgtgcatag ccatgcagcc
//
