LOCUS       NZ_CP093287             3684 bp    DNA     circular CON 25-MAY-2024
DEFINITION  Escherichia coli strain EC-14-2-9 plasmid pEC-14-2-9-5, complete
            sequence.
ACCESSION   NZ_CP093287
VERSION     NZ_CP093287.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26549971
            Assembly: GCF_022605345.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Qin,S. and Deng,R.
  TITLE     EC-14-2-9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Qin,S. and Deng,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2022) Institute of Drug Discovery & Development,
            ZhengZhou University, 100 Science Avenue, ZhengZhou, HeNan 450001,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022605345.1-RS_2024_05_25
            Annotation Date                   :: 05/25/2024 05:14:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,082
            Genes (coding)                    :: 4,822
            CDSs (with protein)               :: 4,822
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 90 of 260
            Pseudo Genes (incomplete)         :: 175 of 260
            Pseudo Genes (internal stop)      :: 58 of 260
            Pseudo Genes (multiple problems)  :: 57 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093287.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC-14-2-9"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC-14-2-9-5"
                     /geo_loc_name="China: Zhengzhou"
                     /lat_lon="34.76 N 113.65 E"
                     /collection_date="2014-12-19"
                     /collected_by="Zhengzhou University"
     gene            join(3604..3684,1..342)
                     /locus_tag="MNY25_RS25805"
                     /old_locus_tag="MNY25_25805"
     CDS             join(3604..3684,1..342)
                     /locus_tag="MNY25_RS25805"
                     /old_locus_tag="MNY25_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_039023181.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVLKHGKTIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1127..1429)
                     /locus_tag="MNY25_RS25810"
                     /old_locus_tag="MNY25_25810"
     CDS             complement(1127..1429)
                     /locus_tag="MNY25_RS25810"
                     /old_locus_tag="MNY25_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="WP_039023182.1"
                     /translation="MVDFYISAIKMDSAGQHIEFVKVHKINRDGSWNTAGSISSRKFIS
                     ELISMGKASFMTVVKNSSTGKWNLGAKVEAMDDGYITTDPNKTKLDNLGNLPKFV"
                     /name="DUF3892 domain-containing protein"
     gene            complement(1493..1786)
                     /locus_tag="MNY25_RS25815"
                     /old_locus_tag="MNY25_25815"
     CDS             complement(1493..1786)
                     /locus_tag="MNY25_RS25815"
                     /old_locus_tag="MNY25_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609279.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023317864.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLDVSQSTLSRLLN
                     GKTDLSYEMALRLSKVVGRSAESWVNMQTQFSLSKSRKSLAAELEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            2123..2404
                     /locus_tag="MNY25_RS25820"
                     /old_locus_tag="MNY25_25820"
     CDS             2123..2404
                     /locus_tag="MNY25_RS25820"
                     /old_locus_tag="MNY25_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032440460.1"
                     /translation="MIKNIKHKGLRSFAEEGSTAGIQPSMAKRLRMILTTLSTATCIQD
                     VVDVRALGCHPLKGERKGEYSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2862..3008)
                     /locus_tag="MNY25_RS25825"
                     /old_locus_tag="MNY25_25825"
     CDS             complement(2862..3008)
                     /locus_tag="MNY25_RS25825"
                     /old_locus_tag="MNY25_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_073545459.1"
                     /translation="MHEPPVQSDCCTLSGNYHLESNPVRHAQAPLAAATGKKVYKRLLI
                     QSS"
                     /name="replication initiation protein"
     gene            3413..3604
                     /locus_tag="MNY25_RS25830"
                     /old_locus_tag="MNY25_25830"
     CDS             3413..3604
                     /locus_tag="MNY25_RS25830"
                     /old_locus_tag="MNY25_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_039023180.1"
                     /translation="MSKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2900..3272)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.467"
                     /coverage="91.0"
                     /evalue="1.28e-167"
ORIGIN
        1 aagttaagtt acacctctgt tttaaagcac gggaagacca ttaaagttat tgatacttca
       61 gagcttatta gagtttttgg ttctcttgat ggtgtcatcg atactgtaaa atatgatgta
      121 aaaagtgacg caggatctac aggtgttaac agcgttggtt tacatgattt acaacataga
      181 attgctttac tagaagctga aaacgatggt cttaaagggg ctgttaaggc tcgagatgag
      241 catattgatt cccttcgtca ggctatgcag cttctggagc acaagcacga accttcttca
      301 ccacccgatt ccccgtggtg gaaattctgg aaaaaatcct gactccgtca tgcttccgtt
      361 ttcctctccg gccatcccgg ggagttcctg aaccttccca aaaatcgcca gatctctgaa
      421 cgctcctcac gactaaaaat acccacacac tacgcaaaat taaaattttg cagatttgta
      481 tttaatatca gtagcttact ttcctgaagt ttcggttttt aaattttatt tacatagatc
      541 cgactctatg acgactcgtc atagagtccc cggcgagtcg tcatagagtc ggtaacgact
      601 cagagctata aaaacaatga gttaataacg acccgactct agacccgact ccccgtttgg
      661 ttgtttttta gactcggcga ctctgggtac gactctagtg aagagtcgcc ggtgaatcgt
      721 gtggtgagtc gtccggcgac ttgccatgac gtgattgccg gccgtcgacg aacacgattc
      781 ctgaacgtgc tgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg ccacgcaata
      841 aattgcgtcc ccgttcaccg ttttcggatg ggaagaacgc cggaaacagg acttaccctg
      901 gctgaatggc gtgaattttc gcgctatgca cttgcgtgca tactcatgca tgccgtaaaa
      961 acagagcctg cgcgtttctg acgggttttc gggtggtttg ttgcctgttt taccggtttc
     1021 ctgtcagaaa cgccctgagg ccgttctggc ggtgcgtaca attatgactt tatgttaaat
     1081 cattagggag gggattctgc tggaacttag tggatgttat tgcatatcag acaaatttcg
     1141 gcagatttcc caagttatcg agtttagttt tgtttggatc tgtggttatg tagccatcgt
     1201 ccatcgcttc cactttggct cctaggttcc acttaccagt agagctgttc tttacaactg
     1261 tcataaagct ggcttttccc atgctgatca gctcgctgat gaatttgcga gagctgattg
     1321 agccggcagt gttccatgag ccatctcggt ttattttatg aacttttacg aattcgatgt
     1381 gctgaccagc actgtccatt ttgattgctg agatatagaa atctaccatg gttgcctcct
     1441 gatggctgct gggtgtttta cgtaacaaaa gcccgcgttc tgcgggcttt tttcatggaa
     1501 acattggatg caagtgttcc agctctgccg ctaaagattt gcgacttttt gacaggctga
     1561 attgggtctg catgtttacc cacgactctg cagagcggcc tacgaccttc gacaatctaa
     1621 gtgccatttc atagcttaag tctgttttcc catttaacag tctacttaag gtagactggg
     1681 atacatcaag tttatcggct aactctgcgg caggtatttc gaacggttcc atgaacattt
     1741 ctctgataaa ctcaccagga tgaacaggat cgtaaagatt tatcatgcct tcctctgttt
     1801 gttttgtatt tgattataca aaaaaattac tgcctttcag tatgcgcatt gcacaatgtg
     1861 caagttcatg atattgacaa aaaaagcagt cagctttagg atactcgttg gagtcaaagc
     1921 tactgcgtat gtatgggctt ttatggcctt ttcaaggcgc agtattttac ccttaggatt
     1981 cgttgaagtc aaatgaggtg attgacctca agaacaaact tacgtcaatt gttttaataa
     2041 agcaaggggg caaaatggaa ctaattaaaa tgattttaaa atatagatac aggtatagac
     2101 gaaaaaaata tggggattgt agatgataaa aaacataaaa cataaaggac ttaggagctt
     2161 tgctgaagaa ggtagcaccg ccggtattca gcccagtatg gcgaaaagac ttcgcatgat
     2221 tttgactacg ctaagcacag caacttgcat tcaagacgtt gttgacgtta gggcgttagg
     2281 gtgccacccc ctaaaggggg agcgtaaagg cgagtattcc gttgaagtta atgggaactg
     2341 gcgggtagtt ttcaaggttg aggaaccaaa cgtcttagac gttgatcttg aagattatca
     2401 ttagttatca ataagtaagt tgctaatcca tccaagtgaa agtacggatg gattagggac
     2461 tttattaaaa aagtaacctc tggagaaagt atgagatcct ttatggtttt attgttcccg
     2521 ttgattagcg taactattgc tggagtccta gcttttcgtg gtattgatgg ctggggatgg
     2581 ttcttgttag ctgctgtact ttgtgcaggg gctgatactg tccagaagca tgtgtctacc
     2641 aataaaaagg agcctgctgg tgagtgagct gtcttggttc ttccttgact gctttgaatg
     2701 ataaagcgta tcttactcgc cgagacgaaa aaaccgcctg gggaggcggt tttttcgagg
     2761 gttcagtaag ttggggaact tctgaaccgt ggtaacaggg tgtacaagac cgctgaaacc
     2821 aaattctgtc ctttcagttt agccgtagtt gggctgagac ttcaagaact ctgtatcagt
     2881 aatctcttgt acaccttctt accagtggct gctgccagtg gcgcttgtgc gtgtcttacc
     2941 gggttggact caagatgata gttaccggat aaggtgcagc agtcggactg aacggggggt
     3001 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt ctggcgtgga
     3061 atgagataaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     3121 agagcgcacg agggagccat cagggggaaa cgcctggtat ctttatagtc ctgtcggggt
     3181 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg
     3241 gaaaaacggc cggtgtgcgg ccttctccct ctccgtgctg tctgtcctat gtcgttatgt
     3301 gtgtattgcg accgctcgcc gcagccgaac ggcaggagcg tagcgactga gtgagcgagg
     3361 aagcggaaaa gagaacatac ctacactgag cactgacgct ctgaggcctc ttatgtcgaa
     3421 acaacagaaa acagcgctga acatggctaa attcatccag aatcagtctc tgctcctgct
     3481 ggagaagctt aacgagcttg atcttgatgc agaggccgac ctttgtgaga aactccacga
     3541 tgatgcggag catcttttcc gtacactgtc ctcgagactg gatgaattac aggatggtaa
     3601 ctgatggcca gagtgtctat tagtgaagcg gcacggcttg ttaaggttag tcgccccaca
     3661 atttacaaga tgattaacag tggg
//
