LOCUS       NZ_CP093273            10123 bp    DNA     circular CON 30-APR-2024
DEFINITION  Klebsiella oxytoca strain C-21-18 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP093273
VERSION     NZ_CP093273.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26549876
            Assembly: GCF_022605305.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Ota,Y., Nukui,Y. and Saito,R.
  TITLE     Klebsiella oxytoca from Tokyo Medical and Dental University
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Ota,Y., Nukui,Y. and Saito,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45, Yushima, Bunkyo-ku 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-JUL-2021
            Assembly Method                   :: Unicycler v. 0.4.8 JULY-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 397.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022605305.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 04:36:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,790
            CDSs (total)                      :: 5,674
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 43 of 95
            Pseudo Genes (incomplete)         :: 59 of 95
            Pseudo Genes (internal stop)      :: 25 of 95
            Pseudo Genes (multiple problems)  :: 25 of 95
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093273.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="C-21-18"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="unnamed2"
                     /geo_loc_name="Japan: Tokyo"
                     /lat_lon="35.70180090 N 139.76431050 E"
                     /collection_date="2021-02-04"
                     /collected_by="Tokyo Medical and Dental University
                     Hospital"
     gene            join(9946..10123,1..2)
                     /locus_tag="MN553_RS28835"
                     /old_locus_tag="MN553_28835"
     CDS             join(9946..10123,1..2)
                     /locus_tag="MN553_RS28835"
                     /old_locus_tag="MN553_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(30..560)
                     /locus_tag="MN553_RS28840"
                     /old_locus_tag="MN553_28840"
     CDS             complement(30..560)
                     /locus_tag="MN553_RS28840"
                     /old_locus_tag="MN553_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515243.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLSDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPSGKK"
                     /name="hypothetical protein"
     gene            complement(567..1298)
                     /gene="mobC"
                     /locus_tag="MN553_RS28845"
                     /old_locus_tag="MN553_28845"
     CDS             complement(567..1298)
                     /gene="mobC"
                     /locus_tag="MN553_RS28845"
                     /old_locus_tag="MN553_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004146447.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1298..3259)
                     /locus_tag="MN553_RS28850"
                     /old_locus_tag="MN553_28850"
     CDS             complement(1298..3259)
                     /locus_tag="MN553_RS28850"
                     /old_locus_tag="MN553_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_032489604.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGSAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPNFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QPEARRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4745..4894)
                     /locus_tag="MN553_RS28855"
                     /old_locus_tag="MN553_28855"
     CDS             complement(4745..4894)
                     /locus_tag="MN553_RS28855"
                     /old_locus_tag="MN553_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_010891188.1"
                     /translation="MKKAKAIFLFILIVSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4979..5236)
                     /locus_tag="MN553_RS28860"
                     /old_locus_tag="MN553_28860"
     CDS             complement(4979..5236)
                     /locus_tag="MN553_RS28860"
                     /old_locus_tag="MN553_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_010891189.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKPWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(5246..6931)
                     /locus_tag="MN553_RS28865"
                     /old_locus_tag="MN553_28865"
     CDS             complement(5246..6931)
                     /locus_tag="MN553_RS28865"
                     /old_locus_tag="MN553_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032427631.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSFSGDALSSAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADTVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPALQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNITTLNGRITSTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNAKLKAAQASLNAMNDALSRDKAALSGAMESRKQKEKKAKEAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7258..7503
                     /locus_tag="MN553_RS28870"
                     /old_locus_tag="MN553_28870"
     CDS             7258..7503
                     /locus_tag="MN553_RS28870"
                     /old_locus_tag="MN553_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515249.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7625..8191
                     /locus_tag="MN553_RS28875"
                     /old_locus_tag="MN553_28875"
     CDS             7625..8191
                     /locus_tag="MN553_RS28875"
                     /old_locus_tag="MN553_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein K"
                     /protein_id="WP_010891191.1"
                     /translation="MDKRTTLVALVSIIFFTSGCDDQKKGNGNAESTQITKNDITTQLK
                     LDKTQQPDDIEKKIKEIQANSTPEKIKKFNELKDDDPKILRESVVSEIKKKLPLLVDEA
                     TLMTDVSTDGGTFSYKYVTKGISASTMESDVWKDAMQKNIKNSYCSDDARLKVFRELFP
                     EGVIYNYYLSDKLIYTYKALPSICS"
                     /name="protein K"
     gene            8277..8540
                     /locus_tag="MN553_RS28880"
                     /old_locus_tag="MN553_28880"
     CDS             8277..8540
                     /locus_tag="MN553_RS28880"
                     /old_locus_tag="MN553_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_010891192.1"
                     /translation="MAAYTSETAKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
                     EITSNGNDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="protein L"
     gene            8886..9149
                     /locus_tag="MN553_RS28885"
                     /old_locus_tag="MN553_28885"
     CDS             8886..9149
                     /locus_tag="MN553_RS28885"
                     /old_locus_tag="MN553_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515247.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRSGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            9139..9438
                     /locus_tag="MN553_RS28890"
                     /old_locus_tag="MN553_28890"
     CDS             9139..9438
                     /locus_tag="MN553_RS28890"
                     /old_locus_tag="MN553_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_021515246.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHILYDVDDQARKVRVLAVVGQRQNPHTVS"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9500..9877)
                     /locus_tag="MN553_RS28895"
                     /old_locus_tag="MN553_28895"
     CDS             complement(9500..9877)
                     /locus_tag="MN553_RS28895"
                     /old_locus_tag="MN553_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515245.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETQERDGLRLPGETEASVSEALISEIKALREEVAGLRQEMQAMRLLEH
                     KPETADTHPETSRRWWHWGKR"
                     /name="hypothetical protein"
     BGC             246..10123
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4062..4642
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(571..1298)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.473"
                     /coverage="100.0"
                     /evalue="7.29e-144"
ORIGIN
        1 aacagcaccg gccatgccgg tgctgccgtt tattttttgc cggacggcca ccaccggcgg
       61 gcgggtttgt cctccggcag ttcaggcacc ggcacgttaa aatagcggct gttatattcc
      121 cgcatcagct ccgctttcag cttctcgttc accgcgcttt ccccgtgccg ctcgcttacc
      181 acctcaatca tcgcgttaat ctgctccggt gagcacttcc cggcatcaac cacctgcaca
      241 ccgttctcga tggcgaaccg ctccaccttg cgatagaggt aatcactcag cttcattcct
      301 gtgggtttgt cccgttccca ttgcggattt tcaccggtga aaaagtacgt ggctttttcg
      361 tcgctgttga gccgctcgtc atacatcccg atggatttca tcatcgccag atgctgggcc
      421 cggtcgacaa aacagaaaac atacgccccg gtctgccagg cgagcaccac catatcttca
      481 agatgctcgc tggtcggcat gatgcagcgc tcgccgtccc ggtcctccgc gtcttcaata
      541 aataccatgt acagcgccat aatccctcac tccgttgtca taaatttaaa gcacgcaaac
      601 agccgttcac gatccagctc cgccgttttg cgggcctcgc cggtctgtgg attcacgatt
      661 tcatccaccg acaggatcgc ccgctgcacc ctgacctgcg tcgcccggtc cgggcagatg
      721 taccagattt catcgatgcc gttcgcccgg cggttaaaca gatacccgga caccacctcc
      781 tgatagcgcc tgcggctctt cacggtgcgc tccacctcaa aagccaccgt tctgccgtcc
      841 agctccgcca gcgcgtccgg caccttcatc cctttcaggg caatacggtg caggtaccgc
      901 cagccggtgg ccccccgggc ctccatggcg aggcgcaggg actgcacggc cagccggtgc
      961 ggcagctgcg ccgccgacac ccgcgacggc tgaaacggaa taatgtccgt cagcggctcg
     1021 tcctcgtcga acgagaacgc caccccgtgc ggcgtcagcc cccacacgct gacctgacgg
     1081 ccgtcaaccg ggtatttcgc ccggaccagc agccctttcg ccaccaggct gtccaggaag
     1141 cggtaaaccg gctgtaccgc cgcaatcccc agccgtcgcg caaccagctc cgccgtggaa
     1201 cacagctctt cccgcagcca gcgcagggtc agccgtgttt tctccgcgtt ccgcgcctgc
     1261 cgcttcgcgt ggctgacgtt gtatcgctcc agtgccatca gtacagcccc gcaaaatcat
     1321 catcacccgg ccgctgccgc ctggcctccg gctggtactc cgccagcggg tccgttttcg
     1381 ccagcaccgg gaaggttttc agcggaattt cccggcgatc aacccgcagc gggctgctga
     1441 acgccagccg cgccacgccc agcccggtca tcaccgcgaa cccgtcaggc agatgttgca
     1501 gggtgttcac gtcaaacagc ggccgcgcct ccttctggat gaagctgtcc ccgctgatat
     1561 gctccacgtt ccccgcctcc cggctggccc ggcggcgctc cacgtccacc cggatttccc
     1621 cggtctggcc cgccgcccac tgcgcggact cctggctggc cgcccggtag aaccagcgga
     1681 tcggcgtgtt atccagcacc gtggttttca caaaatcagc gggcagatcc tggccgcact
     1741 gcccgaagtc gcccagcgac tggtgcgcga gcagcaggtt gcagtcccgg ctgcgcagcg
     1801 tccccagcgc gttcagcacg tacttcgaca gcaggtattt aatctcatcg agcatgatgc
     1861 tcgcgtgcgg ccacctccgg aactcatccc gcgcaatgat gatttgtgcg cagcgggcaa
     1921 acagcatctt ctgcacccgg atcaccgcct catcgtccat cgagccgatg acgtacaggc
     1981 agccgccgcc gttgattatc cccgccacgt cgatgccgct gtccgtctgc aggacgctca
     2041 ggtcggccac gttctccagc ttggtgatca gccccttcac gtcctttttc agggcctccg
     2101 gcagcgcgta cgccgccgcc agcacctgct gaatgtttgc cccctgcgga aactgctccg
     2161 caatcagctt cgccgctttc tgctcgctga tgcggtagtg gtccgccacg tcgccggtct
     2221 cactcaggtt aaacccggca accagcagat tcttcagggc gtactggtcg agatcgcgga
     2281 acagattcag ctgcgccggt ttccccggcc gcagatccag cagggtgaag tttgggcagt
     2341 gcgcccggaa gacgtgcggc gcccacgcgt cccctttggg gtcgaagtac accaccgcgt
     2401 ccccgaaatc gcgtacgcag cgaatgagcg cgttggtggc catcacgctc ttgccgctcc
     2461 cgggcaggcc gaggatctga atgttggtct tgcgccaggt atcacgcggg accagcaccg
     2521 gctggccctg ctcatcctta cccagaaaca ccgcctgctg tttccgggcc tgctccgtgg
     2581 aaaaatcggc cagcgcgttg accggtaccg gctccccgaa atgcaggtgg cggacgtcgg
     2641 tcatttcacc ggtccgctgg ctgctgcgcc ggctctcctg gttcagtttc gcgttcaccg
     2701 cctcgtcagc agccgcaaaa tgaccgtcaa gcagcaggaa cgccagcagc cgcgccaaca
     2761 caatcccgac aaccagcccg cccgcgcacc aggcaatccc gaacaggtta gccggctgca
     2821 gcaggtaata atctgcgttc gcgtagaggt ccggacgcag gacggtttct ccccagctct
     2881 cgataaacac ggcgacaacc agccccccca gaagggccag accggcgctg ccggtcagcc
     2941 agtacgcgta gttccagaca tgccgccgtg ccctgaccat ccgcccgggc gaatccagcg
     3001 aggccagcac cgaaaatggc gcagtccggt tattatcgcg cttatagcga tcggcatact
     3061 cccgtcgctc cttatgactc agccccacaa aacgcatccg tccggcccag agttcagcca
     3121 gaccgaaaaa caggccgata accaggaaag ccccggccag accgtaatac tccctgtaaa
     3181 gccagctgac ccacgggtaa atctgccggg ccagctccgg gatatgccac tgccaggcaa
     3241 gcgaatccgt attaaacatg taatctctct ccttcagacg acgggtttac cggcgtggtg
     3301 tgcagccccc gctgcacaag ccgcggacat gccccaaggg atggcggcgg cgttcacgtc
     3361 cgtggtgact gcgttcagct ttcaccacgg aggccagcct ctttccgccc catgcggaaa
     3421 aggcgcagcc ccggaggaac aacgagcacg ccccagcgtg tgagaggaaa aggcggcacc
     3481 ccagtgcggt cttttccggt gttccggccc ccgtcagagt tcgccatagc gagcggctga
     3541 ccacccgcaa aatcagaggc agctttgctg cgtcgtcagg tgcgtccagc accgtggatt
     3601 tttgtgggtg gtcgggcgtg agcctgacgg gggccgggtg aatttctggc gtggccggcg
     3661 tccagccgga cagagccaga aagaggggga aagaccgtgc gtcggtggcg tccagccagc
     3721 gtaagcaggg gatttccccc tgaagtcagc cacgccccag cggggatgac ttgcatttct
     3781 ccgcaaggag aaaaaagcaa cagagatagg ttaacgtaaa atgttcagcg aaaaaccgac
     3841 atggttcacc tatcctgata attgatcgtc agggaataga aagacgtaat caggggacaa
     3901 tatcccacat cagcgcgtcc ggtgaccgtc ttgcaggtgc cggggaaatt gcgcctcctc
     3961 gctcactcgg tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa
     4021 agttacccac agattccgtg gataagcagg ggactaacat gtgaggcaaa acagcagggc
     4081 cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc agagttcaca taaacagacg
     4141 cttttccggt gcatctgtgg gagccgtgag gctcaaccat gaatctgaca gtacgggcga
     4201 aacccgacag gacttaaaga tccccaccgt ttccggcggg tcgctccctc ttgcgctctc
     4261 ctgttccgac cctgccgttt accggatacc tgttccgcct ttctccctta cgggaagtgt
     4321 ggcgctttct catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag
     4381 ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt
     4441 tcacttgagt ccaacccgga aaagcacggt aaaacgccac tggcagcagc cattggtaac
     4501 tgggagttcg cagaggattt gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa
     4561 agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc cagttaccac
     4621 ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcccca ggtggttttt
     4681 tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4741 ttttctactg aaccgcgatc cccgtcagtt cagaagagga cgatggtgcc accgttccac
     4801 cctgaacatc ccggatatag tttgcctgac atgcgaccag aagaaagcct gaaactatca
     4861 atataaaaag aaaaatagcc tttgcctttt tcatatacct tccttgtatt cagaggcttc
     4921 ggcattcatc cgtgaacacc ggaagcctgt atttcaactc atccttgata acttttgctt
     4981 accagttacc atcccggtaa acaaaggata caaagtaatc aaatttacta atatcgatta
     5041 catttttaaa gtgtggctgt aatatcgaaa cccatggttt ttcaacatca aaccaaccat
     5101 tattaatatt atcctttaaa ggcatcccca gactttcaat gacagaacca tcatcaccga
     5161 agtcttttga gtattcaccg cctttaaact cttcggtttt cttatcaaac caatgaatat
     5221 gtaatttaag ccccatattt acctcttaaa gatatttttt aatgtttcgt tttggatccg
     5281 gccctttaac ctgcttaccc gtttttggat cgaatgcgcc gaggtgttcg ccatcactgg
     5341 cgcggtatcc ttcaagctca ccgtgctggg aatcccattc ataaatttta cgctttttat
     5401 caccatacca tcgggcccgc ttaccacctc ctccttgttt aggggtttta ggttttccct
     5461 ctttcaactc tcccaacccc ttaatatctt cagtcttggg atcaggaaaa taatcatggc
     5521 cataatcttt agttcccttg cgaggctttt tcttttcctc atttaattta ttttctgcct
     5581 ccttcgcttt tttctccttt tgtttccggc tctccatcgc cccggaaagc gcagccttat
     5641 ctctggataa ggcatcattc attgcgttta aagatgcctg agcagctttt aatttcgcat
     5701 tctgattctg aatatcctta tcaagatagc gaacctgatc ctgccacccg gagccatatt
     5761 catgaggagg gttataagtc acaaaatcat ccttattcgc ctccgcctct ttcacctttt
     5821 tatcagcttc ctggacagca gccctggcgc caggaatcgc tttttctgtc gatgtaatcc
     5881 tgccgtttaa agtcgtaata tttttatctt cggcatccag ctcagctttc gctttatcat
     5941 attctctttt cagcccctct tccggatgag cggcgtccca tgcctgctgg cgacgctttt
     6001 cttcctcctg acgctgtttt acctgtgccg gggtaagaac atctgtcacc gacacataga
     6061 ccggcttctg tccggtctct ttcgggaaac gaataacggc ctcacgggag ttaccaccgg
     6121 ccgtaaaacc agccggacgt gaatcacctt tttcagcaac aatgcctttt ggcggggctt
     6181 tcgctgccgg aacacctttc ggtacgctca cctgcagagc cgggagaccc ggaatagaca
     6241 cactgaatac ccccggacgt tttgtcggtt tcgcatccac cacaggaaca ctcattggcc
     6301 gacccgcgac aaccgcaata tgctgccgct catccttcac cacatccaca acccgttgtc
     6361 tgacacgaac cgtcgcttgg tccagcggta aagtacttgc cggagtctcc gtcaccgtat
     6421 cggccggtaa tgacgtcata atttttgaca tcattttcgg atcatctttt gcaatctcag
     6481 aaggcagtac accatagatc gcaatccccc acagaccaaa cttaaacgga cctttcaggg
     6541 cagccagcac atcggcaacg gctgacgaca gcgcatcgcc ggaaaaggat aaagccagtc
     6601 cttcagcccc cggtgtagcc agggcaggca gaccgaaggc catcgcggtg gccgaagact
     6661 gtccaccacc agagctaccg ctatgattgc cgctgttacc accattacca ccgggtttac
     6721 tcccgccgtt accaaactcc cccggattat agttatgaat atctccattc ggcgtatgcg
     6781 tcgtacccca gcccggcccg ctgtttggat ccgtaccacc acctgatgaa gggccacctt
     6841 taccagacgt accgttcaca ttcccactgg cctgaccacc attatgtccc agacctgaat
     6901 tacccggacc tcgaccgtct ccaccactca tatcgttttc ctctttgaca tttaaaacaa
     6961 taaattaaaa ccaaaatact gtatatatac acagtatgat tattacctta ctcctccagt
     7021 tttcaggtgt caatatatga gattagttct catcttcagg cacaaaaaag cgcccggagc
     7081 cgcctgtaag cagatcgcgt cttttcccga aaatttctca tggcaacaaa ttttattatt
     7141 tgagatattc atcacactta cttttatatt ggtatagatg tacctgaaag cgaaatggca
     7201 tgaggagagg acctgcggtt aaactgttcg ggaaacctga gacgcccgga ggccactatg
     7261 acgcacctga aactgactcc cgctgaagaa aagcgcctgg cggacgcctt tgtcgctcag
     7321 gcactggcca tgggcctgaa agggaacaaa acgctgccaa aggcggtaaa acaacaggtg
     7381 cgggaaaggg tgcttcgtgc cattcaggac gaacgtgaag ccaggcgggc gcaggaaatg
     7441 cgtcgggaga aatacgaggc ggcggaaacc accttcacct ggcagccgca gcgccgccgg
     7501 tgaaaacgat ttaaggttct actttcaggt gtatccactt cggaagcatt ttagggatat
     7561 ggtattatcg aaaatcatgc tttcggcaat ccaatgggtt tatacgattt aaaaagggaa
     7621 aaacatggac aaaaggacaa cgttagtagc tctggtttct atcatctttt tcacttctgg
     7681 atgtgatgac cagaaaaaag ggaatggcaa cgctgaatcg acacaaatca ctaaaaacga
     7741 tataacaact caacttaagt tagataaaac gcaacaacct gacgatatag aaaagaaaat
     7801 aaaagaaatc caggccaact ccacccctga aaaaataaaa aaattcaatg aattaaaaga
     7861 tgacgaccct aaaatcctac gagagtcggt tgtttcagaa attaagaaaa aactaccact
     7921 attggttgac gaagccacat taatgacaga tgtatcaact gacggaggta cattctcata
     7981 caaatatgta accaaaggga tttcagccag cacgatggaa agtgatgtgt ggaaagatgc
     8041 catgcaaaaa aacataaaaa atagctactg ctctgacgat gccagactaa aagtattcag
     8101 agagctattt cctgaaggtg tcatttacaa ctactactta agtgacaaat taatctacac
     8161 atacaaggca cttccatcga tttgttccta aagtatttac cataacgtcg caagctgggc
     8221 gccccagcgg gcggcttttc tatatctgtc ctataaattc agaaaaggaa agctaaatgg
     8281 ctgcatatac cagcgaaaca gcgaagtttt tacaaagcgt cagtagcgat caaacatggt
     8341 ggaagtcacg atataatcca ggagacagcg ttccccattc tggcatttat cgatgtacag
     8401 tctgcggaaa agagatcacc agtaacggca acgacccgtt cccgccgcaa aaccaccatc
     8461 agcacagtca gcaacaagca atcaaatggc aactgattgt cagaacggac actaaaggtg
     8521 accgtttcgg aatcaaatga ttgagagcca tccggagctt tttttgtacc tgaaactcga
     8581 agatatttac cataacatcc cttatcggta ccgccagtaa ggcactggcg gcgtttccgg
     8641 cggctacggg ataaaacaag caacaaccca cccggaaaac cgccggaaac gcgcccggta
     8701 aattttatcc taatgcatgc ctatgcacgc aactgcatag catatgtttt gacgtaaaat
     8761 ggatgccgta agcccttttt ttaccgactt tctgcttcat cagcctgact gacacgacca
     8821 tattctttca gaccgccccc tgctaaactt cttcatatca ccgcattaac ccgggaggct
     8881 ccgcaatgag caacactaca cactacgaaa acgccaactt cctgcgggaa ctggctgaaa
     8941 acctgccccg catactgccg gaaggtggcc cggacaaagc tgcgctgttg caacgcctgg
     9001 ctaacgaaga actggcccag gcagagtatg aagatcaggt tcgcgctaaa gtgaccgccg
     9061 cccgcgctga cacgcgctct ggcatgacca cggaacaact tcgccagaga ctgcatggac
     9121 gctatcagga actacgcgat gccgtatgag gtggaatgga agcagggagc catcgaggat
     9181 gtgacagcat tgtttgacta cattgcggaa aattcatccc tgtgggatgc gcagaacgtt
     9241 accgatcgcg ttctcgctgc ggcggacaaa ctggccgact tcccgcgcct ctatgaagtg
     9301 gactcacgct atggcgagga cgttcgccgg atcagcctga tgggacagca tatcctctat
     9361 gatgttgatg accaggcccg gaaagtccgg gttctggctg tggtcggcca gcgccagaat
     9421 ccccataccg ttagctaata accgatgacg ggggcgcaat tcattgcgtg gccgtcgccg
     9481 gttggacaag cgcagcgcgt cagcgttttc cccagtgcca ccaccggcgc gacgtttcgg
     9541 gatgtgtatc tgctgtctcc ggtttgtgtt ccagcagtct catggcctgc atttcctgcc
     9601 gcagtccggc tacctcttcc cgcagcgctt taatctccga aatcagtgcc tcagacaccg
     9661 atgcttcagt ctcacccggc agtctcaacc cgtcccgctc ctgagtctca ccctgtctta
     9721 attcaccgta cacccggata agctcgctgg tatccaccac ccgaccacca tcagcttccg
     9781 tacggtatga tacgcggcct ttcgccatat cacgatacag agaactccgg gctttccccg
     9841 tcagttccgt cgcctgtctc accgtcaccc acgccattgt ctcacctccg gttttgagac
     9901 agtatacccg acaggaagca caccacaccc ggaaggacag aaaacatgac gaagcaggaa
     9961 aaaactgccc tcaatatggc taaattcatt aaggaccagt cgctgctgtt gctcgagaaa
    10021 ctgaacgagc tggatatgga taccccggcc gacatgtgcg agcggcttca cgaggatgcg
    10081 gaaacactct ggcaggtgat gcgggataag ctcgggaagg aat
//
