LOCUS       NZ_CP093262             2683 bp    DNA     circular CON 02-APR-2024
DEFINITION  Moellerella wisconsensis strain W17-3 plasmid pW17-3-b, complete
            sequence.
ACCESSION   NZ_CP093262
VERSION     NZ_CP093262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26537322
            Assembly: GCF_022591735.1
KEYWORDS    RefSeq.
SOURCE      Moellerella wisconsensis
  ORGANISM  Moellerella wisconsensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Moellerella.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Moellerella wisconsensis and Escherichia marmotae
            isolated from wild game meat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x, 120x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022591735.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 05:45:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,268
            CDSs (total)                      :: 3,161
            Genes (coding)                    :: 3,099
            CDSs (with protein)               :: 3,099
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 28 of 62
            Pseudo Genes (incomplete)         :: 44 of 62
            Pseudo Genes (internal stop)      :: 26 of 62
            Pseudo Genes (multiple problems)  :: 24 of 62
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093262.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Moellerella wisconsensis"
                     /mol_type="genomic DNA"
                     /strain="W17-3"
                     /isolation_source="Food: Wild boar meat"
                     /db_xref="taxon:158849"
                     /plasmid="pW17-3-b"
                     /geo_loc_name="Poland"
                     /collection_date="2021"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            complement(1..645)
                     /locus_tag="MNY68_RS16315"
                     /old_locus_tag="MNY68_16315"
     CDS             complement(1..645)
                     /locus_tag="MNY68_RS16315"
                     /old_locus_tag="MNY68_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrD1"
                     /protein_id="WP_012634451.1"
                     /translation="MEKHFINEKFSRDQFTGNRVKNIAFSNCDFSGVDLTDTEFVDCSF
                     YDRNSLEGCDFNRAKLKNASFKSCDLSMSNFKNISALGLEISECLAQGADFRGANFMNM
                     ITTRSWFCSAYITKTNLSYANFSRVILEKCELWENRWNGTVITGAVFRGSDLSCGEFSS
                     FDWSLADFTGCDLTGGALGELDARRINLDGVKLDGEQALQLVESLGVIVHR"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrD1"
     gene            complement(1606..2046)
                     /locus_tag="MNY68_RS16320"
                     /old_locus_tag="MNY68_16320"
     CDS             complement(1606..2046)
                     /locus_tag="MNY68_RS16320"
                     /old_locus_tag="MNY68_16320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015063446.1"
                     /translation="MSQLFRLASPEDYEYIEELNLWELSFDKRPVQGVRCEDPVIGAEK
                     YNQGREKFKALASRHKKHWKPNGLTFSELIRWAIECGTPEQCQLVLALYHCTNDDDYQA
                     MGIQLSRSIDNANVSPIIFFTGKNQRLLGDISMLKQLEEKVR"
                     /name="hypothetical protein"
     ARG             complement(5..645)
                     /name="qnrD1"
                     /gene="qnrD1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrD1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(740..895)
                     /name="Col3M"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.089"
                     /coverage="100.0"
                     /evalue="2.29e-71"
ORIGIN
        1 ttatcggtga acaataacac ctaaactctc aacaagctga agcgcctgct ctccatccaa
       61 cttcactcca tctaaattaa ttcgccttgc atcaagctcg ccaagcgccc cacccgttaa
      121 atcacaacca gtaaaatcag ccaaagacca atcaaacgat gaaaactccc cacaagaaag
      181 atcggagcca cgaaacacgg cgccagttat cacagtgcca ttccagcgat tttcccacag
      241 ttcgcacttt tctaatatga ctctagaaaa attagcgtaa ctaagatttg tcttggttat
      301 ataagcacta caaaaccatg accttgtagt tatcatattc ataaaattag cccctcgaaa
      361 atcagctcct tgagctaaac actcactaat ttcaagacct aaggcgctaa tgtttttaaa
      421 attactcatt gataaatcgc agcttttaaa gctagcgttt tttagtttgg ctctattaaa
      481 atcacaccct tccaagctat tcctgtcgta aaaactacaa tcaacaaatt cagtatcagt
      541 taaatcaacc cctgaaaaat cacaatttga aaaggcaata tttttaactc tattccccgt
      601 aaattgatct cgtgaaaact tttcattgat aaagtgcttt tccatacatg ctacacctgg
      661 ttatttatac agagcaatca ttgtacatta atttgaacaa ccttaaactc tagctaaaat
      721 tgattcatac aatccctttt atgttaaacg ggcgagttag ttagcaaaaa cctcccgttc
      781 atccccatta aagcccctcg aaacgatttt aagacacgat caccctataa aacacccaaa
      841 tctacgctga aagtttagaa cggttttgcg ggcttctgag tgcgttttta acatcgtgca
      901 aactgacaag taaaacgatg cgaaatcgct gaaagtcaca aacttgtttt gtggcaagaa
      961 gcgatttgac caaccgagta aaacgagggc ggtaggcttt ttgtttttat cctgatgagc
     1021 gtccagcgaa gaaagggaaa aacaggggga gccgaaacga aatgacacgc cccagcgatg
     1081 gcaagtgaac cccagtgaac gtgtttcgtt ttgggggcaa agccccccgt caagcgaagc
     1141 gtctgaccac cacatttttg agcgaccaaa tacaggggcg aaaaaagggg gtggtcagac
     1201 gaacgcagtg agtttgacgg gcttgggtga atggtttttt tcgcaggcgt ccagccgaag
     1261 aaaatagcca gagggcggag ccttatgtca ttcattttcg tccagaaaat gagtggcatt
     1321 gatactgaaa gtatcatggg gtgcaagtta gtaaaactat gattctaccc attatttcac
     1381 ccgttattct gcccgttata cctaaattgg gtgcaataac gggtaaagta ataggtagaa
     1441 taacaggtgg attaatgggt aagaaataac acaacgtcat cttgtgtcta tctattattc
     1501 tgtatcttat tctatgtatt attctaccta ttacagacga aatagataaa ataagagata
     1561 gaataataga taggataata cataaaataa taggtaaacc taacgtcagc gcaccttttc
     1621 ttcgagctgt tttagcatcg aaatatcacc taataaccgt tgatttttac ctgtgaaaaa
     1681 gatgatcgga ctgacatttg cattatcaat acttcggctt aactgaattc ccatcgcctg
     1741 ataatcatca tcgtttgtgc aatggtataa cgctaaaaca agctgacatt gttctggagt
     1801 tccgcattct attgcccagc ggatcaactc cgaaaaggtc aatccgttcg gtttccagtg
     1861 ttttttatgc cgtgacgcta acgccttaaa tttttcacgc ccttgattat atttttctgc
     1921 cccgatcacc ggatcttcac aacgaacccc ttgaaccggt cgcttatcaa atgacaattc
     1981 ccataaattt aattcttcga tgtattcgta atcttctggc gatgccaaac gaaataattg
     2041 agacatagag acaccctccg atcatgtgtg aacggagaca gtataaggct ttcgagaaat
     2101 tccgcttcct cgacgtgaaa caagttcacg ccttgcggcg agcgttgcga attgaattta
     2161 tgaggtttac caagctccag atgtaacaaa gccccgtgac agcatcacag ggctctggta
     2221 ctctaaatct tgtgcattcg ttgctcagat tagacgtcgc aatccaatct tagcaacgtg
     2281 agaggcgctt gtcacgttct cattaaatac cagccaacga cattaaccca aatgcaccac
     2341 ttgattgact cggacttagc ccggtctccg aaaggataaa caggcaaatt gtggtagtaa
     2401 aacgtaccgg aaaacgaaat tccggctcta cctccgaatc acgctgcggt atcgaaagat
     2461 tacagtgcgt acctgataag agcaccaatc aacggactag ggatttcttg gattgcgaag
     2521 tgtcgaaaga agcccaacct aaccaaactt agccagatta acgagctaag agaccatgac
     2581 ccaataactg atcatggaga aaagcaaaga caagatgaag atgtcgcttc gctccgtttt
     2641 gattagtacc acattggggc attaggcgtt ttttacctag att
//
