LOCUS       NZ_CP093248             2624 bp    DNA     circular CON 02-APR-2024
DEFINITION  Moellerella wisconsensis strain W51 plasmid pW51-c, complete
            sequence.
ACCESSION   NZ_CP093248
VERSION     NZ_CP093248.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26537325
            Assembly: GCF_022592015.1
KEYWORDS    RefSeq.
SOURCE      Moellerella wisconsensis
  ORGANISM  Moellerella wisconsensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Moellerella.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Moellerella wisconsensis and Escherichia marmotae
            isolated from wild game meat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60x, 120x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022592015.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 05:56:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,513
            CDSs (total)                      :: 3,406
            Genes (coding)                    :: 3,341
            CDSs (with protein)               :: 3,341
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 23 of 65
            Pseudo Genes (incomplete)         :: 50 of 65
            Pseudo Genes (internal stop)      :: 24 of 65
            Pseudo Genes (multiple problems)  :: 23 of 65
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093248.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Moellerella wisconsensis"
                     /mol_type="genomic DNA"
                     /strain="W51"
                     /isolation_source="Food: Red deer meat"
                     /db_xref="taxon:158849"
                     /plasmid="pW51-c"
                     /geo_loc_name="Poland"
                     /collection_date="2021"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            complement(540..980)
                     /locus_tag="MNY72_RS17540"
                     /old_locus_tag="MNY72_17530"
     CDS             complement(540..980)
                     /locus_tag="MNY72_RS17540"
                     /old_locus_tag="MNY72_17530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015063446.1"
                     /translation="MSQLFRLASPEDYEYIEELNLWELSFDKRPVQGVRCEDPVIGAEK
                     YNQGREKFKALASRHKKHWKPNGLTFSELIRWAIECGTPEQCQLVLALYHCTNDDDYQA
                     MGIQLSRSIDNANVSPIIFFTGKNQRLLGDISMLKQLEEKVR"
                     /name="hypothetical protein"
     gene            complement(1618..2262)
                     /locus_tag="MNY72_RS17545"
                     /old_locus_tag="MNY72_17535"
     CDS             complement(1618..2262)
                     /locus_tag="MNY72_RS17545"
                     /old_locus_tag="MNY72_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrD1"
                     /protein_id="WP_012634451.1"
                     /translation="MEKHFINEKFSRDQFTGNRVKNIAFSNCDFSGVDLTDTEFVDCSF
                     YDRNSLEGCDFNRAKLKNASFKSCDLSMSNFKNISALGLEISECLAQGADFRGANFMNM
                     ITTRSWFCSAYITKTNLSYANFSRVILEKCELWENRWNGTVITGAVFRGSDLSCGEFSS
                     FDWSLADFTGCDLTGGALGELDARRINLDGVKLDGEQALQLVESLGVIVHR"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrD1"
     ARG             complement(1622..2262)
                     /name="qnrD1"
                     /gene="qnrD1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrD1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2357..2512)
                     /name="Col3M"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.089"
                     /coverage="100.0"
                     /evalue="2.29e-71"
ORIGIN
        1 acgccccagc gatggcaagt gaaccccagt gaacgtgttt cgttttgggg gcaaagcccc
       61 ccgtcaagcg aagcgtctga ccaccacatt tttgagcgac caaatacagg ggcgaaaaaa
      121 gggggtggtc agacgaacgc agtgagtttg acgggcttgg gtgaatggtt tttttcgcag
      181 gcgtccagcc gaagaaaata gccagagggc ggagccttat gtcattcatt ttcgtccaga
      241 aaatgagtgg cattgatact gaaagtatca tggggtgcaa gttagtaaaa ctatgattct
      301 acccattatt tcacccgtta ttctgcccgt tatacctaaa ttgggtgcaa taacgggtaa
      361 agtaataggt agaataacag gtggattaat gggtaagaaa taacacaacg tcatcttgtg
      421 tctatctatt attctgtatc ttattctatg tattattcta cctattacag acgaaataga
      481 taaaataaga gatagaataa tagataggat aatacataaa ataataggta aacctaacgt
      541 cagcgcacct tttcttcgag ctgttttagc atcgaaatat cacctaataa ccgttgattt
      601 ttacctgtga aaaagatgat cggactgaca tttgcattat caatacttcg gcttaactga
      661 attcccatcg cctgataatc atcatcgttt gtgcaatggt ataacgctaa aacaagctga
      721 cattgttctg gagttccgca ttctattgcc cagcggatca actccgaaaa ggtcaatccg
      781 ttcggtttcc agtgtttttt atgccgtgac gctaacgcct taaatttttc acgcccttga
      841 ttatattttt ctgccccgat caccggatct tcacaacgaa ccccttgaac cggtcgctta
      901 tcaaatgaca attcccataa atttaattct tcgatgtatt cgtaatcttc tggcgatgcc
      961 aaacgaaata attgagacat agagacaccc tccgatcatg tgtgaacgga gacagtataa
     1021 ggctttcgag aaattccgct tcctcgacgt gaaacaagtt cacgccttgc ggcgagcgtt
     1081 gcgaattgaa tttatgaggt ttaccaagct ccagatgtaa caaagccccg tgacagcatc
     1141 acagggctct ggtactctaa atcttgtgca ttcgttgctc agattagacg tcgcaatcca
     1201 atcttagcaa cgtgagaggc gcttgtcacg ttctcattaa ataccagcca acgacattaa
     1261 cccaaatgca ccacttgatt gactcggact tagcccggtc tccgaaagga taaacaggca
     1321 aattgtggta gtaaaacgta ccggaaaacg aaattccggc tctacctccg aatcacgctg
     1381 cggtatcgaa agattacagt gcgtacctga taagagcacc aatcaacgga ctagggattt
     1441 cttggattgc gaagtgtcga aagaagccca acctaaccaa acttagccag attaacgagc
     1501 taagagacca tgacccaata actgatcatg gagaaaagca aagacaagat gaagatgtcg
     1561 cttcgctccg ttttgattag taccacattg gggcattagg cgttttttac ctagatttta
     1621 tcggtgaaca ataacaccta aactctcaac aagctgaagc gcctgctctc catccaactt
     1681 cactccatct aaattaattc gccttgcatc aagctcgcca agcgccccac ccgttaaatc
     1741 acaaccagta aaatcagcca aagaccaatc aaacgatgaa aactccccac aagaaagatc
     1801 ggagccacga aacacggcgc cagttatcac agtgccattc cagcgatttt cccacagttc
     1861 gcacttttct aatatgactc tagaaaaatt agcgtaacta agatttgtct tggttatata
     1921 agcactacaa aaccatgacc ttgtagttat catattcata aaattagccc ctcgaaaatc
     1981 agctccttga gctaaacact cactaatttc aagacctaag gcgctaatgt ttttaaaatt
     2041 actcattgat aaatcgcagc ttttaaagct agcgtttttt agtttggctc tattaaaatc
     2101 acacccttcc aagctattcc tgtcgtaaaa actacaatca acaaattcag tatcagttaa
     2161 atcaacccct gaaaaatcac aatttgaaaa ggcaatattt ttaactctat tccccgtaaa
     2221 ttgatctcgt gaaaactttt cattgataaa gtgcttttcc atacatgcta cacctggtta
     2281 tttatacagt gcaatcattg tacattaatt tgaacaacct taaactctag ctaaaattga
     2341 ttcatacaat cccttttatg ttaaacgggc gagttagtta gcaaaaacct cccgttcatc
     2401 cccattaaag cccctcgaaa cgattttaag acacgatcac cctataaaac acccaaatct
     2461 acgctgaaag tttagaacgg ttttgcgggc ttctgagtgc gtttttaaca tcgtgcaaac
     2521 tgacaagtaa aacgatgcga aatcgctgaa agtcacaaac ttgttttgtg gcaagaagcg
     2581 atttgaccaa ccgagtaaaa cgagggcggt aggctttttg tttt
//
