LOCUS       NZ_CP093247             3260 bp    DNA     circular CON 02-APR-2024
DEFINITION  Moellerella wisconsensis strain W51 plasmid pW51-b, complete
            sequence.
ACCESSION   NZ_CP093247
VERSION     NZ_CP093247.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26537325
            Assembly: GCF_022592015.1
KEYWORDS    RefSeq.
SOURCE      Moellerella wisconsensis
  ORGANISM  Moellerella wisconsensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Moellerella.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Biggel,M.
  TITLE     ESBL-producing Moellerella wisconsensis and Escherichia marmotae
            isolated from wild game meat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60x, 120x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022592015.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 05:56:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,513
            CDSs (total)                      :: 3,406
            Genes (coding)                    :: 3,341
            CDSs (with protein)               :: 3,341
            Genes (RNA)                       :: 107
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 23 of 65
            Pseudo Genes (incomplete)         :: 50 of 65
            Pseudo Genes (internal stop)      :: 24 of 65
            Pseudo Genes (multiple problems)  :: 23 of 65
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093247.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Moellerella wisconsensis"
                     /mol_type="genomic DNA"
                     /strain="W51"
                     /isolation_source="Food: Red deer meat"
                     /db_xref="taxon:158849"
                     /plasmid="pW51-b"
                     /geo_loc_name="Poland"
                     /collection_date="2021"
                     /collected_by="Institute for Food Safety and Hygiene,
                     University of Zurich"
     gene            join(3244..3260,1..424)
                     /locus_tag="MNY72_RS17525"
                     /old_locus_tag="MNY72_17515"
     CDS             join(3244..3260,1..424)
                     /locus_tag="MNY72_RS17525"
                     /old_locus_tag="MNY72_17515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_241542969.1"
                     /translation="MSQLFRLASPADYEYIEELNLWELSFDKRPVQGVRCEDPVIGAEK
                     YNRGREKFKALASRHKKRWKPNGLNFSELIRWAIECGTPEQCQLVLALYHCTNDDDYQA
                     MGIQLSRSIDNANVSPIIFFTGKNQRLLGDVSMLKQLEEKAG"
                     /name="hypothetical protein"
     gene            complement(1298..1471)
                     /locus_tag="MNY72_RS17530"
                     /old_locus_tag="MNY72_17520"
     CDS             complement(1298..1471)
                     /locus_tag="MNY72_RS17530"
                     /old_locus_tag="MNY72_17520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_206449904.1"
                     /translation="MNKYYQVYGNMLAEKSSEVYPYIPLCEECVQEHEVVLEGEDTNDE
                     CFDCGSNGKNDN"
                     /name="hypothetical protein"
     gene            1643..2611
                     /locus_tag="MNY72_RS17535"
                     /old_locus_tag="MNY72_17525"
     CDS             1643..2611
                     /locus_tag="MNY72_RS17535"
                     /old_locus_tag="MNY72_17525"
                     /inference="COORDINATES: protein motif:HMM:NF019371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_206449905.1"
                     /translation="MASIYTKPYQKPEDIVESHLVSRNLSFPNQSDKDFASQILNDVSW
                     YKFKAYLYPYLDHPTKNYKANITFSHGLELYRFDHELRSLCNFFILKVETKAKSRIDHS
                     VSSFTNDPFWYLDDDLFTHKTDLTSERGKFVRYLNKSNMEFAEHYRANYMSNKASFRKS
                     APFWITAELITFDSLVRIIDGLLTTKFDVGGNNSLRDFAISMGARSFSEIKSWMKALKE
                     LRNRCSHNSRTWNSNYRLPSGFVDNHNTCLSNSYITHAPTRKNKIYTLLVVLHLTSKDL
                     SVTETPFKDKLKELLQKYSHIPILLDSMGIPSNWELDPVWA"
                     /name="Abi family protein"
     Typing          1123..1278
                     /name="Col3M"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.452"
                     /coverage="100.0"
                     /evalue="1.07e-69"
ORIGIN
        1 tttggcatca ccagcagatt acgaatacat cgaagaatta aatttatggg aattgtcatt
       61 tgataagcga cccgtgcagg gcgttcgttg tgaagatccg gtgatcgggg cagaaaaata
      121 caatcgaggg cgtgaaaaat ttaaggcgtt agcgtcacga cataaaaaac gctggaaacc
      181 gaacggattg aacttttcgg agttgatccg ctgggcaata gagtgcggaa cccccgaaca
      241 atgtcagctt gttttagcgt tataccattg cacaaacgat gatgattatc aggcgatggg
      301 gattcagtta agccgaagta ttgataatgc aaatgtcagc ccgatcatct ttttcaccgg
      361 taaaaatcaa cggttattag gtgatgtttc gatgcttaaa cagctcgagg aaaaggcagg
      421 ttaatgtcat gtttgtttat tatcctgtgt attattccgt atcttattct atctaatttc
      481 tctctaatac acagaataat acagagaata agatacagaa taagagatag acacaagatg
      541 acgttgtgtc atttcttacc cattaatcta tccgttattc tacccattat tctgtccgtt
      601 attttatccg atttaggtat aacggataga ataatgggta aaataacgga tgaaatcatc
      661 attttaccaa tctacatcta tgatactttc agtatcaatg ccactcattt tctggacgaa
      721 aatgaatgac ataaggctcc gccctctggc tattttcttc ggctggacgc ctgcgaaaaa
      781 aaccattcac ccaagcccgt caaactcact gcgttcgtct gaccaccccc ttttttcgcc
      841 cctgtatttg gtcgctcaaa aatgtggtgg tcagacgctt cgcttgacgg ggggctttgc
      901 ccccaaaacg aaacacgttc actggggttc acttgccatc gctggggcgt gtcatttcgt
      961 ttcggctccc cctgtttttc cctttcttcg ctggacgctc atcaggataa aaacaaaaag
     1021 cctaccgccc tcgttttact cggttggtca aatcgcttct tgccacaaaa caagtttgtg
     1081 actttcagcg atttcgcatc gttttacttg tcagtttgca tgatgttaaa aacgcactca
     1141 gaagctcgca aaaccgttct aaactttcag ggtagatttg ggtgttttat agggtgttcg
     1201 tgtcttaaaa tcgttttgag gggctttaat gggggtgaac gggcggtttt cgctaactaa
     1261 ctcgcccgtt taacataaaa gggattatgg gaatgtctta attgtcattt tttccattgg
     1321 aaccacaatc gaagcattca tcattagtat cttcaccttc aagtacaacc tcatgctctt
     1381 gaacgcattc ttcacacaag ggtatgtaag ggtaaacttc acttgatttt tctgccagca
     1441 tgtttccata gacttgatag tacttgttca tacgtcctct ttttaaaaag cttgcaatgt
     1501 atcaagcatt attttaaagt tgcttcacca acacccacga tggttttact atttttagta
     1561 actcaatcag tgggtttttt agtttttata gtacataaaa ttagctagga ttatcatttt
     1621 cataaattga gacttagcta aaatggcatc catttacaca aaaccctatc aaaaacctga
     1681 agatatagtt gaatctcatc tggttagtag aaatttatca tttccaaatc aatcagataa
     1741 agattttgca tctcaaatat taaatgatgt tagttggtat aaatttaaag catatctcta
     1801 cccttatcta gatcatccaa ctaaaaacta taaagcaaac attacattct cccatgggct
     1861 tgaattatat agatttgatc atgagttaag gtctctttgt aattttttca tcttaaaagt
     1921 tgaaacaaaa gctaaatcta ggatagacca ttcagtatct tcatttacca atgacccatt
     1981 ttggtatttg gatgatgatt tgtttactca taaaactgat ttaaccagtg aaagaggtaa
     2041 gtttgttaga tatttaaaca agtctaatat ggaattcgct gagcattaca gggctaacta
     2101 tatgagtaat aaagcatcat tcaggaagtc agctcctttt tggataacgg cggagcttat
     2161 aacttttgat tctttagtta ggataattga tggattacta actactaaat ttgatgtcgg
     2221 tggaaataac tcattacgtg attttgctat cagtatggga gctagatctt tcagtgagat
     2281 caagtcatgg atgaaagcat taaaagaatt aagaaacaga tgttctcata attcaagaac
     2341 ttggaatagt aattacaggt tgccatctgg ttttgttgat aaccataaca cctgtttaag
     2401 taactcctac ataactcatg ctcctactag gaaaaataag atatacacac tattggttgt
     2461 tcttcactta acatccaagg atttaagtgt gaccgagacc ccttttaagg ataagttaaa
     2521 ggagctacta caaaaatatt cacatatacc tattcttctc gattccatgg gaattccttc
     2581 taattgggaa ttagacccag tctgggcata gaaaacctaa tgccccaatg tggtactaat
     2641 caaaatggag cgaagcgaca acgactttga gtcttcgctt ttctccatga tcggtgattg
     2701 ggtcatggct tcttagccgg ctaatctagc taagtctggt taggttgggc ttctttcgac
     2761 ggtgcaatcc aagaaatccc tagtccgttg attggtgctc ttatcaggta cgcactgtaa
     2821 tctttcgata ccgcagcgtg attcggaggc agagccggaa tttcgttttc cggtacgttt
     2881 tactaccaca atttgcctgt ttatcctttc ggagaccggg ctaagtccga gtcaatcaag
     2941 tggtgcattt gagttaatgt cgttgaatgg ttttaaggaa gtagtgacaa gctagcttcc
     3001 cgttgctaag attggattgc gaggtctaat cgtagcaact aatgcaccga ttcagattag
     3061 cagagccgaa gtgatgtcac aatcatttcg gctctgtgcc ttctggagta tcacaaaaaa
     3121 tttccctcat gttttcaact cgcaacgctc gccgcaaggc gtgaacttgt ttcacgtcga
     3181 ggaagcggaa tttcccgaaa gccttatact atctccgttc acacatcatc ggaggggatc
     3241 actatgtctc aattatttcg
//
