LOCUS       NZ_CP093234             1071 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC-16-35 plasmid pEC-16-35-9, complete
            sequence.
ACCESSION   NZ_CP093234
VERSION     NZ_CP093234.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26549900
            Assembly: GCF_022591475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Qin,S. and Deng,R.
  TITLE     Escherichia coli 16-35 complete sequnce
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Qin,S. and Deng,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2022) Institute of Drug Discovery & Development,
            ZhengZhou University, 100 Science Avenue, ZhengZhou, HeNan 450001,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022591475.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 05:06:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,193
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,811
            CDSs (with protein)               :: 4,811
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 84 of 259
            Pseudo Genes (incomplete)         :: 172 of 259
            Pseudo Genes (internal stop)      :: 61 of 259
            Pseudo Genes (multiple problems)  :: 53 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093234.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC-16-35"
                     /isolation_source="ascites"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC-16-35-9"
                     /geo_loc_name="China:Zhengzhou"
                     /lat_lon="34.76 N 113.65 E"
                     /collection_date="2016-07-16"
                     /collected_by="Qin,s."
     gene            complement(18..209)
                     /locus_tag="MNY24_RS25780"
     CDS             complement(18..209)
                     /locus_tag="MNY24_RS25780"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001027495.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVAN
                     QQAVFLGGHYILQGSRNR"
                     /name="hypothetical protein"
     gene            complement(206..628)
                     /locus_tag="MNY24_RS25785"
     CDS             complement(206..628)
                     /locus_tag="MNY24_RS25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="WP_000271685.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSDD
                     ELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEMKR
                     LYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
                     /name="DUF1380 family protein"
     gene            complement(675..1052)
                     /locus_tag="MNY24_RS25790"
     CDS             complement(675..1052)
                     /locus_tag="MNY24_RS25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="WP_241556013.1"
                     /translation="MSFLPYLFGLDFLYAEASVYALAKKMMPEYEGGFWHFIRLPDGGG
                     YMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLWLYHDSGDAGLTHLYRMRD
                     AQLWSHIEFHPECNAIYAALD"
                     /name="antirestriction protein"
ORIGIN
        1 tcaccgcctg ccctgtatca cctgtttctg ctgccctgta agatgtagtg accgcccagg
       61 aaaacagcct gctgattcgc cacaaaatcg cagatttcat caacaatctg cgcggtggtg
      121 agcggtttct gccccatttc acgacgtcgc gcattaatca cggctttgta attgcgcaga
      181 atttcgcggg tttctgtgct gatattcatg acatgtgctc cccgtccagc gcctgtaaca
      241 caacgtccat tgcttcgcgt tcctccctga cgaacgcatc accgtcgccc ccgttctcac
      301 tcccgacggc atacaggcgc ttcatttcac tgcctgccag cgccatcact ttttccagca
      361 tcaccgccgg aactgtcacc tgacggctgg cacgcttttc ttccatcagc tcacgaacaa
      421 cgtcgtgaac aatgctgaca tccgcaccat attcaaaggc atcgtccagc cgctgcatga
      481 cggtttccag ctcatcgtct gacaggttcc agtcctgggc tatgtggcgc acatccccac
      541 gggtcagcac cacggcaaaa acgcattcac tgtccggcac gtctgtatcc agtacatcgc
      601 ggatgatttc tttaacagta cagtacatgt tctcgtctcc tttacagggg cggcattgcc
      661 gccccgtccg ttaatcagtc cagtgccgcg taaatcgcgt tgcattcggg gtgaaattcg
      721 atgtggctcc acaactgcgc atcgcgcatc ctgtacaggt gtgtcaaccc tgcatccccg
      781 ctgtcgtggt acagccacaa ctggcggtta atcacaaggg aagtcagaat gatgcctgcg
      841 gcatcagcac tcacggtacg gtcaaaccag ttttcaccgt tgaccagatg aaaacggtcg
      901 ccatccggca tcatgtaacc gccaccgtcc ggcaggcgga tgaagtgcca gaatccgcct
      961 tcatattccg gcatcatttt tttcgccagc gcgtatacag acgcttcggc atacagaaaa
     1021 tccaggccaa acaggtaagg cagaaaagac agacggtcgc actcttcaac g
//
