LOCUS       NZ_CP093233             4073 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC-16-35 plasmid pEC-16-35-8, complete
            sequence.
ACCESSION   NZ_CP093233
VERSION     NZ_CP093233.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26549900
            Assembly: GCF_022591475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Qin,S. and Deng,R.
  TITLE     Escherichia coli 16-35 complete sequnce
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Qin,S. and Deng,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2022) Institute of Drug Discovery & Development,
            ZhengZhou University, 100 Science Avenue, ZhengZhou, HeNan 450001,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022591475.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 05:06:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,193
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,811
            CDSs (with protein)               :: 4,811
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 84 of 259
            Pseudo Genes (incomplete)         :: 172 of 259
            Pseudo Genes (internal stop)      :: 61 of 259
            Pseudo Genes (multiple problems)  :: 53 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093233.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC-16-35"
                     /isolation_source="ascites"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC-16-35-8"
                     /geo_loc_name="China:Zhengzhou"
                     /lat_lon="34.76 N 113.65 E"
                     /collection_date="2016-07-16"
                     /collected_by="Qin,s."
     gene            join(2040..4073,1..114)
                     /gene="mobQ"
                     /locus_tag="MNY24_RS25765"
     CDS             join(2040..4073,1..114)
                     /gene="mobQ"
                     /locus_tag="MNY24_RS25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(372..1073)
                     /locus_tag="MNY24_RS25770"
     CDS             complement(372..1073)
                     /locus_tag="MNY24_RS25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1607..1861)
                     /gene="mobC"
                     /locus_tag="MNY24_RS25775"
     CDS             complement(1607..1861)
                     /gene="mobC"
                     /locus_tag="MNY24_RS25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(1863..2039)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2040..2398
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(373..1073)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1954..4073
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.203"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatcatctgt tccgctcgct gggttttaat cgtacctggg aggaatggca gcgcgaggaa
       61 gatgcacgaa cacacaccac cgagaggaga catgatcgcg gttggtcaca gtgaatgtga
      121 acaatatttt gttttgttta acatcgtgtt atgttcgtcg atgttcttcg atgttcatcg
      181 acagggatga atgcaagaag cacactcaca taacgcaagc atagttttgt ttttccgccc
      241 ccaaaaattg gggggtcttt tttcgctgca tcagagcgtt tttgacgcaa tttttcactt
      301 caatagtacc ttcgcactac taacacaaaa aaaacgcagc agggagcgtt ttaggtgcct
      361 taaaatcgat tttagatcgt catcttgatc tgtttgtctt ccttgaagcg aaaatgcagg
      421 gctacaaccg tgcgcccctt tttgatcgtc tcaacagtta caaccagatc gcttttttcg
      481 ttcagttcgg cgatggctgg cttgattatc atcttattca agtctttgaa ttgttgatat
      541 ttatcttcaa cggcaagcat ggttcggaaa tcatcgatag ctatcgttct gtcaccggtg
      601 ctgcggaact gctggagcag ctcgtaaatg cggatgctgt aggtgctgga aaggctggag
      661 acgttcttca cgactacccg cgtaaactgc cccttgagct gtgtcagata cggcatgatg
      721 gcatcggaga aggtgatatc tactttcgcc tcacctttga aatactgcgc ccggtactgc
      781 acccagcgga actcttctcg tttttcctcg ttcttgatga ttactgacct gtcccagagg
      841 cgatctatcg cttccttgag cctagcttca gcgtgctgcc gtcccatgtc aggaaaggca
      901 tcatgaaact ctgcagccgt gatggtcatg gttttttgtt catccggcga aggggaatct
      961 tctccggatt ttaggcggct gatacagacc agcaggaagc gctgttcctg caaagtgagc
     1021 ttgtagcttg cctcgataag ggcgtttgat ttgtaagcta tcagatctga catttcgctt
     1081 gctcgttgct attggtggcg tttttttact gtacgccact tatcatatgg gtggaagttt
     1141 tttttttgca accccctttt tctgtggatt acacccccaa aagctccacc tttcaccccc
     1201 aaaagctcca cctttcaccc ccaaaagctc cacctttcac ccccaaaagc tccacttatc
     1261 ttgccagaac atctttaaaa tcatggtgtt acaagaccta aaaaggtttt aaaaaaaaag
     1321 atctaaaaat aagatcttaa aaacttaacg gcgagcctgt ggataacttg gggtgctgaa
     1381 aacaaaaaac caataaaaac aatgcgttgc aataaagcag aattgcaagt ttcgaataca
     1441 ccccctccaa ccctgtggaa acccagtatt catgcgggtt cagcgcatat cacctgccga
     1501 aaaaccgtgc gtatgtgctc aacctggcga ctgattcact tccgcttcct cgcccactcg
     1561 ctcgctaacg ctcggtcgct ggggctgcgg cgagcggacg atacaatcac tgttctggca
     1621 gcgcctccag ctcgttaatc agcggcagta tgcgcgtcac atcagcctgt cgtgtcacct
     1681 ctgccttcag gaactggagt aaccaggcac gcaactgcac ctcctccggc tttctgacct
     1741 ttgccagcaa tgcagcaccc acaacaacct tctgcgctgt gtccaggctc cggctcttcg
     1801 ccttcaggcg ctgtaatctc gcctcagcat cagcaatctt ctgttcgagc gttctgctca
     1861 tgatgtaact ccgtgcgcga tgaaaaatcg cattttagcg cgtcactggt agtttaaaaa
     1921 ctgagctggc ataatgcacg gcaaatcacg aagtgcgcac ttatacaatc tccactccgt
     1981 ttcgattgtg tgcgccctgc ggggctgaaa gaaaactaca aaaaggcatt acggcagaaa
     2041 tggcgattta tcatctcagc atgaaaatta tctccagaag cagcggctac agcgccgtag
     2101 cttccgctgc ctatcgttcc ggttcactga tgcttgatga gcgtaccgga ctgactcacg
     2161 actacacccg caaaagcggt gtggctgagg ctgtgattct gacccccgcc accgcccctg
     2221 cctggtgtac gaaccgtgcc gaactctgga atgccgttga gaaggccgaa cgccggaaga
     2281 actcacaact tgcgcgggaa attgaactcg ccatccccca cgaactgccg caggatgccg
     2341 cacgggaaac cgttctcgcc tttgtcagag aaaactttgt cagtcagggc atgattgctg
     2401 atgtggcgtt ccatcacatg gataaaacaa atccccatgc gcacatcatg ctgaccacga
     2461 gggctatcgg acctgctggg ttcagcggaa aggtcaggga ctggaacgac cggacacatg
     2521 ccgagacgtg gcgggcatca tgggctgacc atgcaaacag ggcactggcg aacgccggtt
     2581 atcaggaaga gatagaccat cgttcatacg agcgacaggg gcttgagaaa acgcctggta
     2641 ttcacctcgg aaagtctgcc tgtgcgatgg aaacacgcgg tatcgaaaca gaacgcggcg
     2701 aacagaatcg tctgattaac aggcttaacc tggaaataca gatttcccgc accgaactca
     2761 ggaacggagg gctgacagag cctgcccgcc gtgtggctga ccttctgaat atcgggcttc
     2821 ctgacaacgc aacttcgtac acactacgtg acattatcga agcgttgccg aaggacagta
     2881 actcggcatg gaaactcacc agtaatgcca tgtcgatgat ggcagatatg caggcgacgc
     2941 gcagacgctg ggaagagctg aacaccgaac gaaaggaagc gatgagccat gctgccagcc
     3001 tgaaaaaatc atcgccgttt tcgtccggtt tttcccgtat tcccctgatg caatgggcgg
     3061 caccggaata ccgtagtgag cagggaaaaa tccagtctct gagccagcaa atggaaaaat
     3121 tacgacagca ttaccggaag gtggagaaac aggacattcc cgcgagtcag atggcatttg
     3181 aaaaacagtg gcagcagtgg ggggaatccg gccttgcagc tctcagagcc cagttgctta
     3241 aacgtgaaga ggaattgcgc cggaaggaac aggaagatac tgaacgtagt cgcgcggagc
     3301 agctcaggaa aaatgacaat gccgttatgg attcaggggt gctgagagca gtcgttacgg
     3361 ggtatggcga ggctcccatg cctggtaacg gggaaatgac ctgttacctt ctgcttcaca
     3421 accggaacgg agaatatacg ctctggggga acgagctgga gaaataccgc accagaatct
     3481 ttgagagtgt tgatttaatg cgtgacagga gtggttatat ctgtgaccga agcgaaatcg
     3541 gccaacgtcc gccactgcaa cgtgtttatt cctctgcaac ctttgagcaa ctgctgacgc
     3601 aggtttgcca gacatggccg caacataccc gcaatctccg ccagccgaag acatggccgg
     3661 agtctttctg tcttggagaa gaccgccagc cagccatgcc atcaccagcg gcaaggaagg
     3721 tggattttat gcagggacgt ctgccaccga cgctgatgcc ggtcatgagt tctgtcgacc
     3781 gggaaacccg ccagcttcag cttttgctgg tcatgggcgt ggatgactct ctgggcggtg
     3841 ttatccggct taatggtacg ctgtatcccg cgtttgccgt tccctcagcc gataacaggc
     3901 atctggtcat cagtgcgctg accgataagg ggttacggta tgcagggtat ggtgttgccg
     3961 taaatcacga tgcagacagc cacatatcac ctgcaccaga actgatgcag tttcatctga
     4021 aaacccgtga ggcaccgctt tttgctgccg tcaacacacc agaaaaacag ccg
//
