LOCUS       NZ_CP093232             4087 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain EC-16-35 plasmid pEC-16-35-7, complete
            sequence.
ACCESSION   NZ_CP093232
VERSION     NZ_CP093232.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26549900
            Assembly: GCF_022591475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Qin,S. and Deng,R.
  TITLE     Escherichia coli 16-35 complete sequnce
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Qin,S. and Deng,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2022) Institute of Drug Discovery & Development,
            ZhengZhou University, 100 Science Avenue, ZhengZhou, HeNan 450001,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022591475.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 05:06:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,193
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,811
            CDSs (with protein)               :: 4,811
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 84 of 259
            Pseudo Genes (incomplete)         :: 172 of 259
            Pseudo Genes (internal stop)      :: 61 of 259
            Pseudo Genes (multiple problems)  :: 53 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093232.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC-16-35"
                     /isolation_source="ascites"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC-16-35-7"
                     /geo_loc_name="China:Zhengzhou"
                     /lat_lon="34.76 N 113.65 E"
                     /collection_date="2016-07-16"
                     /collected_by="Qin,s."
     gene            complement(join(2428..4087,1..500))
                     /gene="mobQ"
                     /locus_tag="MNY24_RS25750"
     CDS             complement(join(2428..4087,1..500))
                     /gene="mobQ"
                     /locus_tag="MNY24_RS25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_241556012.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            679..933
                     /gene="mobC"
                     /locus_tag="MNY24_RS25755"
     CDS             679..933
                     /gene="mobC"
                     /locus_tag="MNY24_RS25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1465..2172
                     /locus_tag="MNY24_RS25760"
     CDS             1465..2172
                     /locus_tag="MNY24_RS25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(143..501)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          502..678
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1466..2171
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.717"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4..587)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.462"
                     /coverage="99.0"
                     /evalue="1.77e-122"
     Typing          complement(2432..4086)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.457"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gatgcccgcc acgtctcggc atgtgtccgg tcgttccagt ccctgacctt tccgctgaac
       61 ccagcaggtc cgatagccct cgtggtcagc atgatgtgcg catggggatt tgttttatcc
      121 atgtgatgga acgccacatc agcaatcatg ccctgactga caaagttttc tctgacaaag
      181 gcgagaacgg tttcccgtgc ggcatcctgc ggtagttcgc gggggatggc gagttcaatt
      241 tcccgcgcaa gttgtgagtt cttccggcgt tcggccttct caacggcatt ccagagttcg
      301 gcacggttcg tacaccaggc aggggctgtc gcgggagtca gaatcacagc ttcagccaca
      361 ccgcttttgc gggtgtagtc gtgagtcagt ccggtacgct catcaagcat cagtgaaccg
      421 gaacgatagg cagcagaagc aacggcgctg tagccgctgt ttctggagat gattttcatg
      481 ctgagatgat aaatcgccat ttctgccgta atgccttttt gtggttttct ttcagccccg
      541 cagggcgcac acaatcgaaa cggagtggag attgtataag tgcgcacttc gtgattttcc
      601 gtgcattatg ccagctcagt ttttaaacta ccagtgacgc gctaaaatgc gatttttcat
      661 cgcgcacgga gttacatcat gagcagaaca ctcgaacaga agattgctga tgctgaggcc
      721 agattacagc gcctgaaggc gaagagcagg agtctggaca cagcgcagaa ggtcattgtg
      781 ggtgcagcat tgctggcaaa ggtcagaaag ccggaggagg tgcagttgcg tgcctggtta
      841 ctccagttcc tgaaggcaga ggtgacacga caggctgatg tgacgcgcat actaccgctg
      901 attaacgagc tggaagcgct gccaggacag tgattgtatc gtccgctcgc cgcagtccca
      961 gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa gtgaatccgt cgcctgattg
     1021 agcacatacg cacggttttt cgggaggtga tatgcgctga acccgcatga atactggatt
     1081 tccacagggt tggagggggt gtattcggaa tcttcaatcc tgattttttt caacacattg
     1141 tttttattgg gtttttattt tcagcacccc aagttatcca caggctcgcc gttaagtttt
     1201 tagatcttgt ttttaagatc ttgtttttaa tacttgtttt agggtcgaaa ttcctgatta
     1261 aaaacaatgc gttacacgtg tataggtgag ggaatttaag gtaaaggtga tataatttaa
     1321 ggtgcttggg tgaggtaatt taaggtaaag gtgaggagat ttaaggtaat attcacagga
     1381 aaatgggggg tggaaatata attgctcacc aatgctaaag gtgataggtt tttatctcac
     1441 ctcatttttg gcctggtggt taaaatgaca tcaaaccctc tgatagccta taagtccaat
     1501 gcgctggttg aggccagtta caagctaact ttacaagaac agcgtttctt attgctctgc
     1561 ataagccgac ttaactccgg tactgacgta gcttcacctg aattgcaaaa gacaatgacc
     1621 attacagctg cggaatattt tgattcattc cctgatatgg ggcggaaaaa tgccgaggtt
     1681 cagctacagg aagcgatcga tcgtttgtgg gacaggtcga tcattctcaa ggacgacgag
     1741 aagcgcgagg agttccgctg gattcagtat cgggcacagt acgcgagggg agaggggaaa
     1801 gcacaaatca ctttttctga cgcggtaatg ccttatctga cacagttgaa ggggcaattc
     1861 accagagtcg tcattaaaaa tatttccaat ttatcaagaa gttacagcat aagaatatac
     1921 gaaattctcc agcaattccg cagtactggg gaacgtatta tcgccctcga tgattttaaa
     1981 tcatcactga tgcttgatgg taaatataaa gatttcaaaa cgttaaacag ggatctaata
     2041 aaaccctgtg ttgacgaact gaacaaaaaa agcgacctgg ctgtaacggt tgagacgatc
     2101 aaaaaggggc gcacggttgt agccctgcat tttcgcttca aggaagacaa acaaatcaag
     2161 atgacgatct aaaatcgatt ttaaggcacc taaaacgctc cctgctgcgt tttttttgtg
     2221 ttagtagtgc gaaggtacta ttgaagtgaa aaattgcgtc aaaagcgctc taatgcagcg
     2281 aaaaaagaac ccccattttt tgggggcgga aaaacaaaac tatgcttgca ttatgtgtgt
     2341 gcttcttgca ttcatccctg tcgatgaaca tcgaagaaca tcgacgaaca taacacgatg
     2401 ttaaacaaaa caaaatattg ttcacattca ctgtgaccaa ccgcgatcat gtctcctctc
     2461 ggtggcgtgt gttcgtgcat cttcctcgcg ccgccattcg tcccaggtac gattaaaacc
     2521 cagcgagcgg aacagatgat ccggctgttt ttctggtgtg ttgacggcag caaaaagcgg
     2581 tgcctcacgg gttttcagat gaaactccat aagttccggc gcaggtgata tgtggctgtc
     2641 tgcatcgtga tttacggcaa caccataccc tgcataccgt aaccccttat cggtcagtgc
     2701 actgatgacc agctggctgt tatcggctga gggaacggca aacgcgggat acagcgtacc
     2761 attaagccgg acaacaccgc ccagagagtc atccacgccc atgaccagca aaagctgaag
     2821 ctggcgggtt tcccggtcga cagaactcat taccggcatc agcgtcggta gcagacgtcc
     2881 ctgcgtaaaa tccaccttcc ttgctgccag tgatggcatg gctggctggc ggtcttctcc
     2941 aagacagaaa gactccggcc atgtcttcgg ctggcggaga ttgcgggtat attgcggcca
     3001 tgtctggcaa acctgcgtca gaagttgctc aaaggtcgca gaggaataaa cacgttgcag
     3061 tggtggatgt tgaccgattt cgcttcggtc acagatataa ccactcctgt cacgcattaa
     3121 atcaacacgc tcaaagattc tggtgcggta tttctccagc tcgttccccc agagcgtgta
     3181 ttctccgttc cggttgtgaa gcagaaggta acaggtcatt tccccgttac caggcatggg
     3241 ggcctcgcca taccccgtaa cgactgcgct cagtacccct gaatccataa cggcattgtc
     3301 atttttcctg agctgttccg ctcgtcgctc cgcgcgacta cgttctgtat cctcctgctc
     3361 cttccggcgc aattcctctt cacgtttaag caactggact ctgagagctg caaggccgga
     3421 tgccccccac tgctgccact gtttttcaaa tgccgtctga ctcgcgggaa tgtcctgttt
     3481 ctccaccttc cggtaatgct gtcgcaattt ttccatttgc tggctcagcg actggatttt
     3541 tccctgttcg ctacggtatt ccggtgccgc ccattgcatc aggggaatac gggaaaaacc
     3601 ggacgaaaac ggcgatgatt ttttcaggct ggcagcatgg ctcatcgctt cctttcgttc
     3661 ggtgttcagc tcttcccagc gtctgcgcgt cgcctgcata tctgccatca tcgacatggc
     3721 attactggtg agtttccatg ccgagttact gtccttcggc aacgcttcga taatgtcacg
     3781 tagtgtgtac gaagttgcgt tgtcaggaag cccgatattc agaaggtcag ccacacggcg
     3841 ggcaggctct gtcagccctc cgttcctgag ttcggtgcgg gaaatctgta tttccaggtt
     3901 aagcctgtta atcagacgat tctgttcgcc gcgttctgtt tcgataccgc gtgtttccat
     3961 cgcacaggca gactttccga ggtgaatacc aggcgttttc tcaagcccct gtcgctcgta
     4021 tgaacgatgg tctatctctt cctgataacc ggcgttcgcc agtgccctgt ttgcatggtc
     4081 agcccat
//
