LOCUS       NZ_CP093192             8834 bp    DNA     circular CON 20-JUL-2023
DEFINITION  Staphylococcus epidermidis strain TMDU-302 plasmid p4, complete
            sequence.
ACCESSION   NZ_CP093192
VERSION     NZ_CP093192.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26517680
            Assembly: GCF_024204825.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Saito,R., Ayibieke,A. and Yamada,K.
  TITLE     bloodstream S. epidermidis isolates from two Hospitals in Tokyo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Saito,R., Ayibieke,A. and Yamada,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45 yushima, Bunkyo-ku, Tokyo 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembley v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 742.0x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/20/2023 03:47:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,450
            CDSs (total)                      :: 2,366
            Genes (coding)                    :: 2,299
            CDSs (with protein)               :: 2,299
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 34 of 67
            Pseudo Genes (incomplete)         :: 41 of 67
            Pseudo Genes (internal stop)      :: 22 of 67
            Pseudo Genes (multiple problems)  :: 24 of 67
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093192.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="TMDU-302"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p4"
                     /geo_loc_name="Japan"
                     /lat_lon="35.701735 N 139.7642509 E"
                     /collection_date="2015-07-11"
                     /collected_by="TMDU hospital staff"
     gene            join(8524..8834,1..415)
                     /locus_tag="MNU45_RS12180"
                     /old_locus_tag="MNU45_12175"
     CDS             join(8524..8834,1..415)
                     /locus_tag="MNU45_RS12180"
                     /old_locus_tag="MNU45_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002957487.1"
                     /translation="MKHVFKVFLASMLATLLGCTVVTSHHASAAENHPSTKQDTKLVAT
                     YKTSQVDAKTMKQFKSIAKEDPNFHIKKQQGKVVIEELFPNPKKQKSNYTANRSTNNHT
                     RVLNFSDFIGNMDGNNTHNTSNNISTYSKHKGKVYKGPTKDGQHVKKGMHVHCNRFNGT
                     KSDHRYWSKKHPRAYVDFYKSDCWFHASKYHCYSMNNPKGMVKCDGLNQLYHKGVKDCS
                     SWKGKPKHKNWPKTAWYRN"
                     /name="hypothetical protein"
     gene            898..1263
                     /locus_tag="MNU45_RS12185"
                     /old_locus_tag="MNU45_12180"
     CDS             898..1263
                     /locus_tag="MNU45_RS12185"
                     /old_locus_tag="MNU45_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049368612.1"
                     /translation="MKWLKIIILILSLVPIEFIGLFTDYQTGLLIGYIPFIIVAILISI
                     SIFKFGLKNNISIIISRCIGTFLSWECVHWFMNHYEPEFYFEPFMADDFALFLGAIHFI
                     VIMLIYLVIYGFSHRNN"
                     /name="hypothetical protein"
     gene            complement(1826..2557)
                     /locus_tag="MNU45_RS12190"
                     /old_locus_tag="MNU45_12185"
     CDS             complement(1826..2557)
                     /locus_tag="MNU45_RS12190"
                     /old_locus_tag="MNU45_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_018113501.1"
                     /translation="MKITKFFTLFLLFLTITIVLSGCNKDVEHKKNISIKDIINDKKQH
                     MFFSMYTDEDNNNEIESIYLTKNGQMKQIFLGSSEISPSFLLNLDPNEVKKVLDNKKID
                     YQETPYKDVKTYLEVTNEKGNALITFVTTKDISEKKAKKGLGDVLKSVYSNDKQTEGLL
                     LYTNVSTGSIQSAGDAGKYTGQLTNDNLPSLMNESNDESDRLDTIIKLNNKDQKMVNVS
                     SQDKDVIKIKLSDFDTKLDQN"
                     /name="hypothetical protein"
     gene            complement(2651..3517)
                     /locus_tag="MNU45_RS12195"
                     /old_locus_tag="MNU45_12190"
     CDS             complement(2651..3517)
                     /locus_tag="MNU45_RS12195"
                     /old_locus_tag="MNU45_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440631.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_161386845.1"
                     /translation="MSGETVIYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLHL
                     SFDELKELSNYNPETRNINRFVDDLQRVYDKMLDIRYTIRTETKIKKFVLFYKYEIDIS
                     ERYLEIATSPDLKYILNSITNNFTKFELKEMTHLKSTYAKNMFRMLKQYKHSGYMKIRV
                     EDFRERLDIPHSYRMSNINQFVLTPIIRELSTIFNNLKINKIKAKKGRKIEWLEFIFDP
                     EKRIHSKRQPEIKDVGKPRQMISREKTPKWLEERDNPKYQNKYNVDENFEKDKEAFLKQ
                     LKVDWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(4194..5402)
                     /gene="mobV"
                     /locus_tag="MNU45_RS12200"
                     /old_locus_tag="MNU45_12195"
     CDS             complement(4194..5402)
                     /gene="mobV"
                     /locus_tag="MNU45_RS12200"
                     /old_locus_tag="MNU45_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_254106769.1"
                     /translation="MKLAWNTQKNKMSDVKGKEMEQERKGRIKNDEIDLNRTNLNFDLV
                     EDERHLYHRVKDRIEYYKKQGSRVQKNSVVMYSNIITLSKEEADRMGETRTKHYFKTCK
                     DYFSGRFGEANVVSAKVHMDESAPHMHLHFIPVNHQGRLSARTAMNRQAINHIHDELTT
                     HLCQQGFDVERGSTDDNKTYIQDIHEYKKKAKVLSELDQQIETKKQLLRDINDLHDEKE
                     DLTRNIQNLKRKNHFLQEDSQQVQEDIDLYKHLRAISRDDFKKEEEKYQKEIKAYEASL
                     SGLRMNYRDLDKRNQHLTTQNNQMEHHLDMLNTRHQAMNDMVRNDTRILFAGLRCALKD
                     MNIELSAEIKYQRMPEVGIDPDTVDQGALTHAYQGDVSGIDHHIRKASAPIKKETENDL
                     ELE"
                     /name="mobV"
     gene            5857..6288
                     /locus_tag="MNU45_RS12205"
                     /old_locus_tag="MNU45_12200"
     CDS             5857..6288
                     /locus_tag="MNU45_RS12205"
                     /old_locus_tag="MNU45_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002501812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049368610.1"
                     /translation="MSKKQNSKEKILTIRVSQSQYKKIQDLAYIRSMNTTEYIRETAVG
                     NRIKPTVIEYPKEQTTLEDYEEDSKVSELESKINEYEELIQAQSHHIEELEQTVKHYQP
                     QVDTFKRMLTHIDSTGYINFGSYKNDRLLLNQIRNSKRD"
                     /name="hypothetical protein"
     gene            <6403..6795
                     /locus_tag="MNU45_RS12210"
                     /old_locus_tag="MNU45_12205"
                     /pseudo=""
     CDS             <6403..6795
                     /locus_tag="MNU45_RS12210"
                     /old_locus_tag="MNU45_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(6863..7933)
                     /locus_tag="MNU45_RS12215"
                     /old_locus_tag="MNU45_12210"
     CDS             complement(6863..7933)
                     /locus_tag="MNU45_RS12215"
                     /old_locus_tag="MNU45_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002503035.1"
                     /translation="MESYDLYEKKYNYYKELLSLSYSDAVQSLIEKHGEVVDNYYKEKS
                     YNKFLNGEIKRIAYGKYTKTKEGLYCHHILENEYENISNKEYIAHYKYPYEYHHKENLV
                     YCDLIEHLILHALITKETNGDRGFKGLVEFLIPTAEEWYIDGYEPRVLWMQLCRERAFL
                     SEEYTEKLLSDVDELLKDVQVYQELLEEIREEELRKLEHEDRLRQMIEQQELEDEERIQ
                     RHMQNLNISREEYENNEDLIKEEAHRYINKDSIFELKISKKTPRKEVLELLQHYEELYD
                     SSKFYSDEYASSKLNIVKDDLIEELRELSKKVELKLENAKYYKKDGNYNIKVRKGLSFE
                     VTPESANIIIDGKRLQ"
                     /name="hypothetical protein"
     gene            complement(8048..8422)
                     /locus_tag="MNU45_RS12220"
                     /old_locus_tag="MNU45_12215"
     CDS             complement(8048..8422)
                     /locus_tag="MNU45_RS12220"
                     /old_locus_tag="MNU45_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002503034.1"
                     /translation="MKKIVYILGIIIVLGFCYFYVNLIFFDSWTRYSAEKEINNYISNH
                     DTKKLKEISANNKTYQLLKHTKHVTIKGDTDNQGGGHIGYFTSTINGKEGALFVHLNFG
                     LFPEIPKVTKLEVFDKNIYE"
                     /name="hypothetical protein"
     Typing          complement(2653..3517)
                     /name="rep_cluster_1448"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.204"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4198..5474)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.775"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagagtatta aatttttctg attttatagg aaatatggac ggaaacaata ctcataatac
       61 atcaaataac atatcaacat atagtaaaca caaaggcaaa gtatataaag ggccaactaa
      121 agatggccaa catgtaaaaa aagggatgca tgttcattgt aatcgattta acggaacaaa
      181 atctgatcat agatactggt caaaaaaaca tcctagagct tatgtagatt tttataaaag
      241 tgactgttgg ttccatgcta gcaaatatca ttgttattct atgaataatc caaagggtat
      301 ggttaagtgc gatggtttaa accaactcta tcataaaggt gtcaaagatt gctcatcatg
      361 gaaaggtaaa cctaaacaca aaaattggcc taaaacagca tggtatagaa attaaacgac
      421 tgaaagaaca ataaaaaagc aagcgactat caatcatcat agtcgcttgc tttttttatt
      481 ttagattaca tgattgctta attttagaaa aatgttttaa tttttttagc tcctttaata
      541 tttttaggaa gattatatgg gttaaagtag tgataaacac ctgttgaatt tacagacttt
      601 ttacctgcac cgaaataata cgattgccaa acaagttttg aacaatatgt gtgatatgta
      661 tcatttatat tggaatttaa agaatataag gtgtaggggg aaacagattt acaaaagaaa
      721 gactcctatt ataaatatct tttagaatca gaaccttaca gaaaataata taaatgtata
      781 acataataaa aaatagagat cattcatcta ccacttagag acagcgtaga cttctaccta
      841 attatattaa taaaatgata ttgaaggaaa aataatattg aaaagagaaa ttttaaaatg
      901 aaatggttaa aaatcattat attaatttta tctttagttc caatagaatt tatcggatta
      961 tttactgatt atcaaacagg tttattaata ggctacatcc catttataat agttgctata
     1021 ttaataagta tatctatttt taaattcggc ttaaaaaata atataagtat tataataagt
     1081 agatgtattg gaacatttct ttcttgggaa tgtgttcatt ggtttatgaa tcattatgaa
     1141 ccggaatttt actttgaacc ttttatggct gacgattttg cattattttt aggagctatt
     1201 catttcatag ttattatgct tatatattta gttatctatg gtttttctca tcgcaataat
     1261 taactattat gttacttata ttaaaggaca caaagattaa tttaacaatg tttgtgtttt
     1321 tgagtattta tacaaaggca ctacccataa tcagtaatta tgtcaacttg tagtaaaggc
     1381 ttaaacgctg ttaaatcaac gtttaagcct ttgtcttgtg attatcgttt attcaggttt
     1441 tatacatcat tgttctcacg cgttttcata ctttttcatt aaatgaactt ttgtatcttt
     1501 attcaaaaac ggagaatcta ttaaaatcag attctgttat actaaaaaag cacttcccgt
     1561 gcagaaagga ggtgccacat ctaatgattg tcgttctgtt tgttgtgaaa tatatcgtat
     1621 gtccaattat tgttggacgt gttctttatt taataaatca agaacaacga taaaaaagaa
     1681 accccagcat atgctttggt cggcatgctg gggttttcac atccaatgat gtgtctgtct
     1741 gtttgttatt tttagtatat cttgcatgaa gagatttgta aaattatgat gtctaaaaaa
     1801 tacaaaatgt tgaagtaata gaaatttaat tttggtctaa tttcgtatca aaatcagata
     1861 atttaatttt gatgacatcc ttatcttgag aagaaacatt caccattttt tgatctttat
     1921 tatttaattt aataattgta tcaagtctgt cagattcatc attagattca ttcattaaac
     1981 taggtaaatt atcgttggtt aattggccag tatacttacc agcatcacca gcactttgta
     2041 tacttcctgt actcacattt gtataaagta ataagccttc ggtttgttta tcatttgaat
     2101 agactgattt gagtacatcg cctagacctt ttttagcttt cttttcagaa atatccttag
     2161 tggtaacaaa tgtaataagg gcattccctt tttcatttgt tacttctaaa taagttttaa
     2221 catctttata gggtgtttct tgataatcaa tctttttatt atcaaggact tttttaactt
     2281 cattaggatc taaattcagt agaaatgaag gagatatttc tgaagaaccc aaaaagatct
     2341 gtttcatttg accattttta gttaagtata tagattcaat ctcgttatta ttatcttcat
     2401 cagtatacat tgaaaagaac atgtgctgtt ttttatcatt aatgatatct ttaatagata
     2461 tatttttttt gtgttcaaca tctttattac atccactcaa aacaattgta attgtcaaaa
     2521 aaagtaagaa taaagtaaaa aatttagtta ttttcattta ttatctcctt ttaatttcaa
     2581 ataaatttaa tatgtatatg tatttataat actaacatta aaaaaatata taccaattac
     2641 tttttttact ttattcttcc caatccactt taagttgctt taaaaaagct tctttatctt
     2701 tttcaaaatt ttcgtcaacg ttatatttat tctgatattt cggattatct cgttcttcta
     2761 accatttggg cgttttctca cgacttatca tctgtcgtgg tttacctaca tcttttattt
     2821 caggctgtcg cttggagtga atgcgtttct caggatcaaa gataaactcc agccattcaa
     2881 ttttacgtcc ttttttagct ttgattttat tgatttttag attattaaat atcgtagata
     2941 gttctcttat gattggagtt agaacaaatt gattaatatt agacattcta taagaatgtg
     3001 gaatatctaa acgttctcta aaatcttcaa ccctaatttt catataacca gaatgcttat
     3061 attgtttaag cattctaaac atatttttgg cataagtaga tttaagatgt gtcatttcct
     3121 ttaattcaaa tttagtaaag ttattagtaa tagagtttaa aatatacttt aaatctgggg
     3181 aagtagcaat ttctaaatat ctttcagata tatcaatttc atatttataa aatagaacga
     3241 attttttaat ttttgtttct gttctaatag tatatcgaat atctaacatt ttatcgtaaa
     3301 ctctttgtaa gtcatctaca aatctgttta tattacgcgt ttctggatta taattgctta
     3361 attcttttag ctcatcaaaa gataagtgta acgtattagt gtcttgctct tttaatttat
     3421 tacacattgc aaaaaataag ttgatttcag tagctgtaaa tcgtcttaaa ggcactaagt
     3481 tcatttcatt tttatatata acagtttctc cactcatatg atactcctct ccttacaaag
     3541 gcataacaat aaaaaaacta tgcctttgta aatttttaaa acacctcata acccttataa
     3601 ttaaggtaac atatagttaa aggcataaca ataaaaaaac tatgccttta atgtggcaaa
     3661 actatgcctt taatgtggca aaactatgcc tttaatgtgg caaaactatg cctttaatgt
     3721 ggcaaaacta tgcctttaac atttcaaaaa ccttgtccct ctaggtctta aacagtactt
     3781 aaaaaggttt taaaaagatt tataaaaata tattaaaaag gtcggagtaa aatacttcaa
     3841 acgcattccg cattcaaata tatttctaaa gaaaccaaca caaaaataaa cttttaaaag
     3901 aatggaggta ttttttaaag tttagaaacc ccaaatatct tcatcaatta atttagaaat
     3961 catactcaca tctatcactt ccaatacaat atctttgcta ccttggaatt cttataataa
     4021 tatagaaatg caaaggtatt gttcctcctt acagaactat ttaataagcg ctgtaagtaa
     4081 ggtaatgatt gttcttaata ggttgatcat acttattaaa tttttatctc ctcttttcat
     4141 gagataagca cttaagcgag tattatactt gtttaagtgc ttattttatt gatttattct
     4201 aattctaaat cattctctgt ctctttttta ataggtgctg atgcctttct tatatgatgg
     4261 tctatccctg acacatcacc ttgatatgca tgtgttaaag ccccctgatc gaccgtatcg
     4321 ggatcaatac cgacttctgg catgcgttga tatttaattt ctgcgcttaa ttcaatgttc
     4381 atatctttca atgcacatcg aagccccgca aacaaaatac gtgtatcgtt tcttaccata
     4441 tcgttcatcg cttgatgacg cgtatttaac atgtctaaat gatgttccat ctgattattt
     4501 tgtgttgtta aatgttgatt gcgcttgtcc aaatctcggt agttcattct aagaccactc
     4561 agagacgcct catacgcttt gatttctttt tgatattttt cctcctcttt tttaaaatcg
     4621 tccctagaaa tcgctctcag atgcttatat aggtcgatat cttcttgaac ttgttgagaa
     4681 tcctcttgta aaaaatgatt tttccgtttt aagttttgaa tatttcgcgt taaatcttct
     4741 ttttcatcat gtaaatcgtt gatatccctc aacaattgtt ttttcgtttc aatttgttgg
     4801 tctaattcac ttaaaacttt cgctttcttt ttgtattcat gaatatcttg aatatacgtt
     4861 ttattatcat cggtactccc acgttcaaca tcaaaacctt gctggcacag atgtgttgtt
     4921 aattcatcat ggatgtgatt aatcgcttgt cgattcatgg ctgtacgtgc tgataatcgc
     4981 ccttgatgat taacaggtat aaagtgaagg tgcatatggg gtgctgattc atccatatga
     5041 actttcgctg aaacgacatt ggcttcgcca aaacgtccac tgaagtaatc tttacatgtt
     5101 ttaaagtagt gctttgtacg cgtctctccc atacggtcgg cctcttcttt cgataacgta
     5161 atgatattgg aatacatcac cacactattt ttctgtacac gtgagccctg ttttttatag
     5221 tactcaattc tatctttcac acgatggtac aaatgacgtt cgtcttcaac taaatcaaag
     5281 tttaagtttg tccggttcaa gtcaatttca tcgtttttga ttctcccttt acgttcttgt
     5341 tccatctctt tgcctttaac atcactcatt ttattttttt gcgtattcca agctaatttc
     5401 aaattgagcg ccctccccga agctttttga tttgtctgtg gttctctact ttcgagaacc
     5461 ttcgcaaata tcattttaca agcgctgtga ttgtctacct tcgacaatgc ttcgcttatg
     5521 taaaatgatg gatactctca ccgctacgcc tcaaacatgt gaggctgatt ccgcaatgag
     5581 agtatagctc gatatactct tacattcaaa tataagagtt atcttcgctc tggcgaggcc
     5641 atcactgcat tcaaaaatat atttttgaat accgttctat agctaacaaa tgacatacgc
     5701 cttatcaaac atatttgttt gataaggcgt atgtcatttg ttagctcata ccacctcgaa
     5761 tcatacatat ttattagcat acatatgtat gttatattaa tataaagata agcacacata
     5821 tgtatgctta ttatatatta atcatgaggt ggttcaatgt caaaaaaaca aaattcaaaa
     5881 gaaaaaatcc taacaattcg tgtaagtcaa tcgcaataca aaaaaataca agatttagcc
     5941 tacatacgta gtatgaatac aacagaatat atacgcgaaa cagcagttgg taatcgtatt
     6001 aaaccgacag tgattgaata tccaaaagaa caaacgacat tagaagatta tgaagaggat
     6061 tcaaaggtta gtgaattaga aagcaaaatc aatgaatatg aggagcttat acaagctcaa
     6121 tcacatcata tagaagaatt agagcaaaca gtcaaacatt atcaacctca agtagacaca
     6181 ttcaaacgta tgttaacgca tatcgatagt acaggatata ttaattttgg gagttataaa
     6241 aatgatcggt tattgcttaa tcaaattaga aacagtaaaa gagactagcc aaatcactag
     6301 tctcttttac tgtttctaat tacactttaa aatctacggt tctgttgcaa agtcagaaag
     6361 ataagtatat aaccttatgt ttctgacttt gcaacagaac ctgcagaggg acatacatta
     6421 gatatttggt tgcgtaagca acgagataat cattcagcat atgtatttat caaacgtctc
     6481 attaaacaat ttggtaaacc tcaaaaggta attacagatc aggcaccttc aacgaaggtc
     6541 gcaatggcta aagtcattaa agcttttaaa cttaaacctg actgtcattg tacatcgaaa
     6601 tatctgaata atctcattga gcaagatcac cgtcatatta aagtaagaaa gacaagatat
     6661 caaagtatca atacggcaaa gaatacttta aaagatattg aatgtattta tgctctatat
     6721 aaaaagaacc gcaggtctct tcagatctac ggattttcgc cataccacga aattaggcat
     6781 atgttagaca gttgagggaa caacgacatg ataaaatggt aattagctat atttttctaa
     6841 ctttgcaaca gaaccgtttt atttattgta atctttttcc atctataata atgtttgcac
     6901 tttcaggcgt aacctcaaaa ctaagccctt tacgtacctt aatattatag ttcccatcct
     6961 ttttataata tttagcattt tctaatttta attctacttt tttagaaagt tcacgtagct
     7021 cctctattaa gtcatctttt acaatattta gttttgaaga agcatattca tcactgtaga
     7081 atttactaga atcatataat tcttcataat gttgaagtaa ctcaagaact tcttttctag
     7141 gtgttttctt gcttatttta agttcaaaaa tactatcttt atttatatat ctatgtgctt
     7201 cttctttaat taaatcttca ttattttcat attcttctct actaatattt aagttctgca
     7261 tatgtctttg aattctttct tcatcttcta attcttgttg ttcaatcatt tgtcgaagtc
     7321 tatcttcatg ttctaatttt cgtagctctt cttctcttat ttcttctaac aattcctgat
     7381 atacttgaac atcctttaat agttcatcaa catctgaaag taatttttca gtgtactctt
     7441 ctgataagaa tgctctttct ctacataatt gcatccataa tactctaggt tcataaccgt
     7501 ctatatacca ttcttcagcc gttggtataa gaaactcaac aagtccttta aaccctctat
     7561 ctccatttgt ctctttggta attaaagcat gtaatatcaa atgctctatt aagtcacaat
     7621 atactagatt ctctttatga tgatattcat aagggtattt ataatgagct atatattctt
     7681 tgttagatat attttcatat tcattttcca atatgtgatg acaatataaa ccttcttttg
     7741 tttttgtata tttaccgtat gcaattctct ttatttcccc atttaaaaat ttgttataag
     7801 atttttcttt atagtagtta tctacaactt caccatgttt ttcaattaaa gattgaacag
     7861 catcactgta tgataaactt aacagctctt tataataatt atattttttt tcgtataaat
     7921 cataagattc cataaaacta ttttctccca acattgtata atactgtttt caattgatta
     7981 ttaatttaat tttctaaatt tgcaacataa ctagaataat taacacgaaa aatgagtagc
     8041 ataattgcta ctcataaata tttttatcaa aaacttccaa cttggttact ttaggaattt
     8101 caggaaacaa accaaaatta agatgtacaa aaagcgctcc ttctttacca ttaatcgtac
     8161 tagtaaaata ccctatatga cctccacctt gattatctgt atctcctttt attgtcacat
     8221 gtttagtatg tttcaaaagt tgatatgttt tattattcgc ggatatttct tttagtttct
     8281 ttgtatcatg atttgaaata taattattta tttctttttc agcagaataa cgtgtccaac
     8341 tatcaaaaaa aattaaattt acataaaaat aacaaaagcc taaaactata ataataccaa
     8401 gtatataaac tatttttttc atttaaagac tccttatctc ctcaaaatat ttaagttttt
     8461 atggtaagtt aaagttgcat aataatccaa caaaaacact ttaaaaggag ttgtccgaaa
     8521 caaatgaaac atgtatttaa agtcttttta gccagtatgt tagccactct tttaggttgt
     8581 actgtagtaa cttctcatca tgcctctgca gctgaaaacc atccttcaac caaacaagat
     8641 actaaattag tcgctaccta taagacttct caagttgacg caaaaacaat gaaacaattc
     8701 aaatctatag ctaaagaaga tcctaatttc catattaaaa aacaacaagg taaagttgtc
     8761 atagaagaat tattccctaa tccaaagaaa caaaaatcaa attatacagc taaccgtagc
     8821 acaaacaatc atac
//
