LOCUS       NZ_CP093191            10412 bp    DNA     circular CON 20-JUL-2023
DEFINITION  Staphylococcus epidermidis strain TMDU-302 plasmid p3, complete
            sequence.
ACCESSION   NZ_CP093191
VERSION     NZ_CP093191.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26517680
            Assembly: GCF_024204825.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Saito,R., Ayibieke,A. and Yamada,K.
  TITLE     bloodstream S. epidermidis isolates from two Hospitals in Tokyo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   Saito,R., Ayibieke,A. and Yamada,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45 yushima, Bunkyo-ku, Tokyo 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembley v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 742.0x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/20/2023 03:47:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,450
            CDSs (total)                      :: 2,366
            Genes (coding)                    :: 2,299
            CDSs (with protein)               :: 2,299
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 34 of 67
            Pseudo Genes (incomplete)         :: 41 of 67
            Pseudo Genes (internal stop)      :: 22 of 67
            Pseudo Genes (multiple problems)  :: 24 of 67
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093191.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="TMDU-302"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p3"
                     /geo_loc_name="Japan"
                     /lat_lon="35.701735 N 139.7642509 E"
                     /collection_date="2015-07-11"
                     /collected_by="TMDU hospital staff"
     gene            complement(join(9911..10412,1..422))
                     /locus_tag="MNU45_RS12130"
                     /old_locus_tag="MNU45_12125"
     CDS             complement(join(9911..10412,1..422))
                     /locus_tag="MNU45_RS12130"
                     /old_locus_tag="MNU45_12125"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="WP_145436688.1"
                     /translation="MINNLLSMNDVNITDINSIFDKADYYEKALKDEKVIKDLEGKILA
                     TLFYQPSTRTRMSFTSAMYRLGGQVIDMGGPKVARTDDYFDESMSDTIKYIEQYSDIMV
                     MRHFQNYTVEKASEITHIPMINAGDGTNEHPTQALLDLYTMSKELNGLKDKVIGIGGDI
                     NCRVIRSIVMGLEKFDIKKIVFLLPNGEELNSDILNLLKNTDYSIVHNVERVLELADIL
                     DIIPFELPDFNSAYSEKVDEKPSLENNLIVSKEKFNDKNRIPILSPGPREAELSSDTDN
                     MDNVIFTKQAYNGLLIRMALLYYFLQ"
                     /name="aspartate carbamoyltransferase"
     gene            complement(415..1305)
                     /locus_tag="MNU45_RS12135"
                     /old_locus_tag="MNU45_12130"
     CDS             complement(415..1305)
                     /locus_tag="MNU45_RS12135"
                     /old_locus_tag="MNU45_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486791.1"
                     /GO_function="GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="WP_254106770.1"
                     /translation="MNSKVIKYTADFDEKRYWERVKRNLGWLGNTDEEAKKRQKKISEV
                     VIGIAGCGGIGGAVAERLIRLGVRHIKVADPDHFELSNINRQFGASLDNIGRNKAEVVG
                     ESIFNISRDVNVDVYPEGINDNTADDFVDGCDYVLDKIELFELEARYALHRAFKKNERC
                     KFMLTVPVFGHRAFFFKWTKDSMSAEDYFDIKPGSELDEQNTRKIVYSLFPEYPQFPSK
                     ERLDDWLIHNKECPIFAGCPPLAQGLLVERLSFEITDLKDIPEYKELPSTPSYSMFDSA
                     SWKTKIVEREENNID"
                     /name="ThiF family adenylyltransferase"
     gene            complement(1446..2123)
                     /locus_tag="MNU45_RS12140"
                     /old_locus_tag="MNU45_12135"
     CDS             complement(1446..2123)
                     /locus_tag="MNU45_RS12140"
                     /old_locus_tag="MNU45_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436976.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_145436686.1"
                     /translation="MYEDFADNILLIGHKTRLTMLIELSSGISLPAGELAKLAGVKPQT
                     ASEHLSKLVEANLISVNTWGRHRYYKIDNDKVVDAINALSVISPPMSSKSLKETTKKEK
                     LSYIRTCYGHIAGQIGVKFTEALLENDYLKEKEEYYKLTQKGKNWLKRIGMETEKSMYS
                     KSIPKHIDWTERKNHMAGPIALRITEKMIELSWIENSEINRCLNLTKKGKSAFKTYLNM
                     DLP"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            2235..3629
                     /locus_tag="MNU45_RS12145"
                     /old_locus_tag="MNU45_12140"
     CDS             2235..3629
                     /locus_tag="MNU45_RS12145"
                     /old_locus_tag="MNU45_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_162162596.1"
                     /translation="MYSSKYFDHLVVFTAGIGMFLSTLDSGIINVALPTLSKYFNVDTS
                     FITWSVTLYTLLLTGTIIIFGRLSDKYNRLNIYSIGLTVFLISSILCGLSNNVVELITF
                     RGLQGIGAAMLQGTATAIITTTIPENRQGPALGTLSILLGIGPVLGPSIGGFLISVGNW
                     RWIFWINIPFIFIGLIGCILLKKYIKEQNSVSIHLDIRGNLLLFLSIFCLLISLTSWSY
                     HSMLDISVYGNFLIFIVTFGLFIVWELKTHHPIIDLRLFKNISFSSPIFAIFVFGGTTS
                     LGFIIPPYILEKVNHLSSWQIGLVNLTSPLGLVLISKISGKLISRIGNIILMTIGLVIM
                     IAAYTNLGLLQYILNPITMSLLLLLYGIGGGFFLPSNTSAIMGTVSQDMQGTVGATQRM
                     VQNIGIAFYTAVTSLFINHSSNSDKLLEGSSHAWLFASLTLFLALLPFSVKLLKHKFFE
                     EKYDSK"
                     /name="MFS transporter"
     gene            complement(3671..4123)
                     /locus_tag="MNU45_RS12150"
                     /old_locus_tag="MNU45_12145"
     CDS             complement(3671..4123)
                     /locus_tag="MNU45_RS12150"
                     /old_locus_tag="MNU45_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162162597.1"
                     /translation="MPKKQSSKEKILTVRVSQAQYKKIQDLAYIRSMNVTEYIRQTAVG
                     NRIKPTVIEYPKEQIIMDDSHSKNDINDWHKVIEQLKKDNEALKSNNQELQQHIHQLED
                     EIDPMRQENDVFHHLLQHFDSTAFMDFSTYRDDRPLKNAIKRLKEQ"
                     /name="hypothetical protein"
     gene            4578..5786
                     /gene="mobV"
                     /locus_tag="MNU45_RS12155"
                     /old_locus_tag="MNU45_12150"
     CDS             4578..5786
                     /gene="mobV"
                     /locus_tag="MNU45_RS12155"
                     /old_locus_tag="MNU45_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_049392809.1"
                     /translation="MKLAWNTQKNKMSDVKGKEMEQERKGRIKNDEIDLKRTNLNFDLV
                     EDERHLYHRVKDRIEYYKKQGSRVQKNSVVMYSNIITLSKEEADRMGETRTKHYFKTCK
                     DYFSGRFGEANVVSAKVHMDESAPHMHLHFIPVNHQGRLSARTAMNRQAINHIHDELTT
                     HLCQQGFDVERGSTDDNKTYIQDIHEYKKKAKVLSELDQQIETKKQLLRDINDLHDEKE
                     DLTRNIQNLKRKNHFLQEDSQQVQEDIDLYKHLRAISRDDFKKEEEKYQKEIKAYEASL
                     SGLRMNYRDLDKRNQHLTTQNNQMEHHLDMLNTRHQAMNDMVRNDTRILFAGLRCALKD
                     MNIELSAEIKYQRMPEVGIDPDTVDQGALTHAYQGDVSGIDHHIRKASAPIKKETENDL
                     ELE"
                     /name="mobV"
     gene            complement(6545..6700)
                     /locus_tag="MNU45_RS12160"
                     /old_locus_tag="MNU45_12155"
     CDS             complement(6545..6700)
                     /locus_tag="MNU45_RS12160"
                     /old_locus_tag="MNU45_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="WP_018113591.1"
                     /translation="MEHISKLGEAIVNTISASQSGDSAELAKSIVNIAANGIGVISDVA
                     KAFGLM"
                     /name="beta-class phenol-soluble modulin"
     gene            7208..8074
                     /locus_tag="MNU45_RS12165"
                     /old_locus_tag="MNU45_12160"
     CDS             7208..8074
                     /locus_tag="MNU45_RS12165"
                     /old_locus_tag="MNU45_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494364.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_018113587.1"
                     /translation="MTGETVVYKNDLNLVPLRKFTSTEINLFFAMCNKLKEKETNTLHL
                     SFDELKELSNYSPKTRNINRFVKDLDDVYKKMLELTIRYEDDEVIERFILFNHYRIHKK
                     DQYLEISTSPNLKHILNSITSNFTKFELKEMTNLKSTYSKNMFRILKQYKHTGYMKIKI
                     DDFRERLDIPKSYRMTDINKNVLKPIVTELNAIFNNLHINKIKAKKGRKIEWLEFIFDP
                     EKRIHNKRQPQMTNVGKQRQYINREKTPEWLNEGRLGQSQEIHNEDSKLRQDREAFQRQ
                     LEEKWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            8742..9308
                     /gene="lepB"
                     /locus_tag="MNU45_RS12170"
                     /old_locus_tag="MNU45_12165"
     CDS             8742..9308
                     /gene="lepB"
                     /locus_tag="MNU45_RS12170"
                     /old_locus_tag="MNU45_12165"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503824.1"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009306 - protein secretion [Evidence IEA];
                     GO:0006465 - signal peptide processing [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="WP_018113588.1"
                     /translation="MKKEFKEWLIAIIVAILLLFLVNNFLFKPYTVSGLSMYPTFNNKD
                     KVVVSKISKSLNHLKSGDVIIFHQNSNNDFIKRLIGKPGDQIEYKNDKLYINKNYIKEP
                     YLDYNKKMNNSSENLTEDFNVSNIKGSKHKMIIPKDKYLVLGDNRTNSIDSRYSEVGLI
                     SKKQIVGKVILRFWPFNDMKYNFKR"
                     /name="lepB"
     ARG             2236..3629
                     /name="mdeA"
                     /gene="mdeA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic; aminoglycoside
                     antibiotic; penam; tetracycline antibiotic; disinfecting
                     agents and antiseptics"
                     /identity="39.8"
                     /coverage="102.43"
     Typing          7209..8074
                     /name="rep_cluster_1439"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4507..5783
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.009"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccaataaag cttgtgtagg gtgctcattt gttccatccc cagcattaat cataggaata
       61 tgggtgattt ccgatgcctt ttcaacagta taattttgaa aatgacgcat aaccatgatg
      121 tctgaatatt gttcaatata tttaatagta tctgacatac tctcgtcaaa ataatcatca
      181 gtacgtgcta ctttaggacc acccatatct atgacttgac cacctaatcg atacatggca
      241 ctcgtaaaac tcattctagt tctggtactt ggttgataga aaagtgtagc aagaatcttc
      301 ccttctaaat ccttaattac tttttcatct tttaaagctt tttcgtaata atctgcctta
      361 tcaaatatac tattaatatc agtaatattc acatcattca ttgataataa gttattaatc
      421 aatgttattt tcctctcttt ctacaatttt tgttttccaa gaggctgaat caaacataga
      481 ataacttgga gttgagggta attctttata ttctggaata tcttttaaat cagtaatttc
      541 aaagctcaat ctttctacta aaagaccttg agctaaaggg ggacatcctg caaagatagg
      601 gcattcttta ttatgaatta gccaatcatc taacctttct ttggatggga attgagggta
      661 ctctgggaaa agggaataga ctatttttct tgtgttttgt tcatctagct ctgaacccgg
      721 cttgatatca aaataatctt cagctgacat tgaatcttta gtccatttaa aaaagaaagc
      781 tctatgacca aatactggaa cagttaacat aaatttgcat ctttcattct ttttaaaagc
      841 tctatgcaat gcgtagcgag cttccaattc aaataattca attttatcta aaacataatc
      901 acaaccgtct acaaaatcat cggcagtatt atcattaata ccttcaggat agacatcaac
      961 attcacatct cttgagatat taaatataga ctctccaact acttctgcct tatttcttcc
     1021 gatattatcc aaagatgcac caaactgtct attaatatta gaaagctcaa aatgatctgg
     1081 atctgctact tttatatgac gaactcctaa tcttattaat ctttcggcta cagcacctcc
     1141 tataccacca caaccagcaa tacctattac gacttcactg attttctttt gacgcttttt
     1201 ggcttcttca tcagtatttc ctaaccaacc taaatttcgt ttaactcttt cccaatatcg
     1261 tttttcatca aaatcagcag tatacttaat cactttagaa ttcattatga acctccaaaa
     1321 tataagattt tgtaacaact acatcactcg acacagagaa aaattgaaat ataagtgttt
     1381 taatacactt tcaataagta cataatcata aaaacgtttt atttgtcaaa atatttcttt
     1441 ttttcttatg ggaggtccat atttaaatat gtcttaaacg cactttttcc ttttttggtt
     1501 aaattaagac atcgattaat ttcactattt tcaatccaag ataattcgat cattttttca
     1561 gttattcgta atgcaatcgg tcctgccata tgattttttc tttcagtcca atctatatgt
     1621 tttggaatag atttagaata catacttttt tctgtttcca ttccaattct ttttaaccaa
     1681 tttttccctt tttgagtcaa tttataatat tcttcttttt ctttaagata atcattttct
     1741 aataaagctt cagtaaattt aacacctatt tgtccagcaa tatgaccata acaagtacgt
     1801 atatatgata gcttttcttt ttttgttgtt tcttttaacg atttgctact cataggtgga
     1861 gatatgacag ataaagcatt gatagcatca acaactttat cattatcaat tttataataa
     1921 cgatgtcgtc cccatgtatt tactgaaatt aaattagctt ctactaattt tgaaagatgt
     1981 tcactagctg tttgcggttt tacaccagct agtttagcta attctccagc tggaagagat
     2041 ataccactag ataattcaat taacatagtt aaacgtgttt tatgtccaat gaggagaata
     2101 ttatcagcaa aatcttcata catataatac atcctttcta ttagtacaat ttaatcatac
     2161 ccatataata tttcggtgtt aaacgaaata ttatatgggt atgattaaat tagttaaatg
     2221 aggaggtatg atagatgtat tcttctaagt atttcgatca tttagttgtt ttcacagcag
     2281 gaataggaat gtttttatcc accctggata gtggcattat caatgtcgca ttacccactt
     2341 tatcaaaata ttttaatgtt gatacttctt ttattacttg gagtgttaca ctatatactt
     2401 tattattaac aggcacaatt ataatatttg gaagattaag cgataagtac aatcgtctaa
     2461 atatctatag cattggttta acagtgtttt tgatatcttc tattttatgt ggactttcaa
     2521 acaacgtggt agagttaatt acttttcggg gtttacaagg cataggtgca gcaatgttac
     2581 aaggaactgc tacggctatc attactacaa ctattcctga aaatagacaa ggaccagcat
     2641 taggaacttt aagtattcta ttagggatag gccctgtttt aggaccaagt attggaggct
     2701 ttttaatatc tgtaggtaat tggagatgga ttttttggat taatattccc tttatattta
     2761 taggattaat aggttgtata cttttaaaaa aatatattaa agaacaaaat agtgtatcta
     2821 ttcatttaga tatacgtgga aatctattat tatttctatc tatcttttgt ttactgataa
     2881 gtttaacttc ttggtcttat cattcaatgc ttgatatatc agtatatggc aatttcttaa
     2941 tctttattgt tacttttggt ttatttattg tgtgggaatt aaaaactcat catcctatca
     3001 tcgatttacg tttattcaaa aatatatcat tttcatctcc tatatttgca atatttgtat
     3061 ttggaggtac aacatcttta ggttttataa tccccccata tattttagaa aaagttaatc
     3121 atctttcatc ttggcaaata ggattagtta atttaacatc tcctttagga ttagtactta
     3181 tttcaaaaat atctgggaaa ttaatttctc gtattggtaa tattatttta atgactatag
     3241 gattagttat aatgattgct gcatatacca atttaggatt attacaatat atattaaatc
     3301 cgataacaat gagtctatta ttattattat atggtatagg tggaggtttt ttcctaccat
     3361 ctaatacttc agcaataatg ggcacagtat ctcaagatat gcaaggtact gttggagcta
     3421 ctcaaagaat ggtccaaaat ataggtattg ctttttatac agcagttact tctttattta
     3481 ttaatcattc atcaaattca gataagctct tagaaggatc cagtcatgca tggttatttg
     3541 catcattaac actattttta gcactattac ctttttcagt taaattatta aaacataaat
     3601 tctttgaaga aaaatatgat tcaaagtaaa aaaagcaagc gactacgata attgatagtc
     3661 gcttgctttt ttattgttct ttcaatcgtt taatcgcatt ttttaaaggg cgatcatcac
     3721 gatatgtact gaaatccata aatgctgtac tatcaaaatg ttgtaataaa tgatgaaaaa
     3781 cgtcattttc ttgtctcatt gggtctatct catcttccag ttgatgaata tgttgctgga
     3841 gttcttggtt attggatttg agtgcctcgt tatctttttt cagctgttca atgactttat
     3901 gccaatcatt tatatcattc ttagaatgag aatcatccat tattatttgt tcttttggat
     3961 attcaatgac tgtcggttta atccgattac ccacagctgt ttggcgtata tattctgtga
     4021 cattcatact acgtatgtag gctaaatctt gtatcttttt gtattgcgct tgactgacac
     4081 gaacggttaa gattttttct ttcgaacttt gtttttttgg cattgaacca cctcatgatt
     4141 aatatgtgat aagcacacat acgtatgctt atctttatat taatataaca tacatatgta
     4201 tgctaataaa tatgtatgat tcgaggtggt atgagctaac atttggcata catattttca
     4261 aacatagttg tttgataagg tgtatgtcat ttgttagcta tagaacggca ttcaaaaata
     4321 tatttttgaa tgccgtaatg gcctcgccag agcgaagata actcttacat ttgaatgtaa
     4381 gagtatatcg agctatactc tcattgcgga atcagcctca catgtttgag gcgtagcggt
     4441 gagagtatcc atcattttac ataagcgaag cattgtcgaa ggtagacaat cacagcgctt
     4501 gtaaaatgat atttgcgaag gttctcgaaa gtagagaacc acagacaaat caaaaagctt
     4561 cggggagggc gctcaatttg aaattagctt ggaatacgca aaaaaataaa atgagcgatg
     4621 ttaaaggcaa agagatggaa caagaacgta aagggagaat caaaaacgat gagattgatt
     4681 tgaaacgaac aaacttaaac tttgatttag ttgaagatga acgtcatttg taccatcgtg
     4741 tgaaagatag aattgagtac tataaaaaac aaggctcacg tgtacagaaa aatagtgtgg
     4801 tgatgtattc caatatcatt acgttatcga aagaagaggc cgaccgtatg ggagagacgc
     4861 gtacaaagca ctactttaaa acatgtaaag attacttcag tggacgtttt ggcgaagcca
     4921 atgtcgtttc agcgaaagtt catatggatg aatcagcacc ccatatgcac cttcacttta
     4981 tacctgttaa tcatcaaggg cgattatcag cacgtacagc catgaatcga caagcgatta
     5041 atcacatcca tgatgaatta acaacacatc tgtgccagca aggttttgat gttgaacgtg
     5101 ggagtaccga tgataataaa acgtatattc aagatattca tgaatacaaa aagaaagcga
     5161 aagttttaag tgaattagac caacaaattg aaacgaaaaa acaattgttg agggatatca
     5221 acgatttaca tgatgaaaaa gaagatttaa cgcgaaatat tcaaaactta aaacggaaaa
     5281 atcatttttt acaagaggat tctcaacaag ttcaagaaga tatcgaccta tataagcatc
     5341 tgagagcgat ttctagggac gattttaaaa aagaggagga aaaatatcaa aaagaaatca
     5401 aagcgtatga ggcgtctctg agtggtctta gaatgaacta ccgagatttg gacaagcgca
     5461 atcaacattt aacaacacaa aataatcaga tggaacatca tttagacatg ttaaatacgc
     5521 gtcatcaagc gatgaacgat atggtaagaa acgatacacg tattttgttt gcggggcttc
     5581 gatgtgcatt gaaagatatg aacattgaat taagcgcaga aattaaatat caacgcatgc
     5641 cagaagtcgg tattgatccc gatacggtcg atcagggggc tttaacacat gcatatcaag
     5701 gtgatgtgtc agggatagac catcatataa gaaaggcatc agcacctatt aaaaaagaga
     5761 cagagaatga cttagaatta gaataaatca ataaaaataa gcactcaggc aagtatgata
     5821 cttgtttgag cgcttatctc aatgaaaaga gtaagtaaaa acttaataag ctggcgacac
     5881 ttattaagag caataataac tttaattact gaactagtct actaattcag taattaggaa
     5941 cgataccttt gtatttctat attattataa gaattccgag gtagcaatga tattgactta
     6001 atatgagcaa attgatattg gaagcaaaag aaacccactc aaacgaacga gtgggttttg
     6061 aaaaatgata cgtattaact tgataaaatt aatcatttta tagtttaaag aaaaggaatg
     6121 cataattata gtaacacaaa ttaatgtttg ttacactagt agtgctgctc aatatataaa
     6181 gtagtaaacc ataactattc ttcgcatctc ttgttaaaaa gttaattttt tgctttgtac
     6241 aaggaaaagc actcaatttt gagtgctttt ttcatattaa aattaaactt taaaaattaa
     6301 gacagcgaca ctgatatgtg tcgctatctt aagatatatt atatgagttc aaaagatata
     6361 tagctattgt agcataatag tgtttttatt aaaaaacaag acgaaatagt ttgaattact
     6421 gaataactta tgtcttaaat cttttaattt atttaagata cgttgttatt aagaaaataa
     6481 aaaaagatat aaatcaacta aataattgat ttatatcttc atatgtattg atttaaaata
     6541 cttactacat taaaccaaac gctttagcaa cgtctgatat tacaccaata ccattagctg
     6601 caatattaac aattgattta gctaattcgg cactatctcc agattgtgat gctgaaatag
     6661 tattaacaat agcttcacct aattttgaaa tatgttccat gaaaaacaaa cctccttaca
     6721 tataaaatca ttactcaaat aaaataacat atattacgat aattctcttc tacttttaaa
     6781 taattgtaat taaatttacg ttttcttaaa aataaataaa gggattgcaa tgttgttact
     6841 attaaaatta ataaaaaaac tttgaatata ataatagcta ttttacatgg aattttttaa
     6901 tatcttttta taaatctttt tataaacctt tttagaagtc gtttgagctt tagagccaca
     6961 gcttatttga gatgcttaag gagtagtttt gccacataaa aggagtagtt ttgccacata
     7021 aaaggagtag ttttgccaca taaaaggagt agttttgcca cataaaagga gtagttttta
     7081 ttataaacct tcttttgtta attgacattg ttatcaaagt gttttaacgt gaaaatatca
     7141 attcaataag gagtatcata aaaaaatatg ctccttattg aaataattat gttgaggtat
     7201 ctatataatg acgggagaaa cagtagtata caaaaacgat ttgaatttag ttcctttaag
     7261 aaaattcacc agtactgaaa ttaatttatt ttttgccatg tgtaataaac ttaaggaaaa
     7321 agaaactaac acacttcatt tatcttttga tgaattgaaa gaattgagta actatagtcc
     7381 gaaaacacgt aatattaata gattcgttaa agacttggat gatgtatata agaaaatgtt
     7441 ggaactaaca attagatacg aagatgatga ggttatagaa agatttattt tatttaatca
     7501 ttatagaatt cataaaaaag atcaatactt agaaatttca acttctccta atttaaagca
     7561 tatacttaat tcaattacta gcaactttac taaatttgaa ttaaaagaaa tgacaaattt
     7621 gaaatctaca tattcaaaaa acatgtttag aatacttaaa caatataaac acactggtta
     7681 tatgaaaatt aaaattgatg attttagaga acgcctagat atccctaaat cttatagaat
     7741 gacagatatt aataaaaatg ttttgaagcc tattgttaca gagttaaacg ctatttttaa
     7801 caatcttcac attaataaaa tcaaggctaa aaaaggacgt aaaattgaat ggctggagtt
     7861 tatctttgat cctgagaaac gcattcacaa taaacgacaa cctcaaatga ccaatgtagg
     7921 taaacagcgt caatatatca atcgtgagaa aacaccagaa tggttaaatg aaggaagatt
     7981 aggacaatct caagaaatac ataatgaaga ttcaaaatta agacaagatc gagaggcatt
     8041 tcaacgtcaa ttagaagaaa aatgggagga ataataactt aaatgagcta catctatatt
     8101 ttgcttttat ttcctttaat aatgggattt ttcttatcac taaaagagaa aaagtatgat
     8161 aaaaaatgac taattcacta ttgtaaagaa aatacagaat tttatgagta aaacttgtaa
     8221 ttttattgca agtgaagaaa ctacagtata tactgaaact aataaatatt gaactcatat
     8281 atcaccgaac cccaacctac tgacataggt tgggttcttt agtggtaagg ctatcttgac
     8341 ttatttattg cggttgttta gccaaaacat gaaaatcgca acaccgcatc ctactataat
     8401 ttgaataaag cattaaaaat ggccactcaa caatttgagt ggcattgaaa tcgttaagga
     8461 gttacttacg ttaaaaaaat taatgttagt tagggacaat tatatgtttt cactttaaca
     8521 cttatttata tttaactcac tcaaaaaata tttaacgctg gaaatttttg ccttttttga
     8581 aacgagtgga acgagttcat tctgttcttg atagtaaatt ttattatttc aataaagaaa
     8641 agacatcata aataatgaac tacttttggt aggatattaa aaatataagt gataacctaa
     8701 aataggataa agtttataaa atctaaaaag gaaattatgt aatgaaaaaa gaatttaagg
     8761 aatggttaat agccataata gtagctatat tattattatt tttagtaaat aattttttat
     8821 ttaaacctta tactgtatca ggattatcaa tgtatccaac gtttaacaat aaagataaag
     8881 tagtagtcag caaaatatcc aaatcattaa atcacttaaa gtctggagat gttattatat
     8941 ttcatcaaaa tagcaataat gactttataa aacgattgat tggtaaacca ggagatcaaa
     9001 ttgaatataa aaatgacaag ttatatatca ataaaaatta tatcaaagaa ccttaccttg
     9061 actataataa aaaaatgaat aactccagtg aaaatttaac agaagatttt aatgtatcaa
     9121 atataaaggg atctaaacat aaaatgataa tacctaaaga taagtattta gttctcggag
     9181 acaataggac aaacagtatt gatagtagat attctgaggt ggggttaata tctaaaaaac
     9241 aaatcgtagg aaaagttatt ttaagatttt ggccttttaa tgatatgaag tataatttta
     9301 agcgatgaat gataaaaaag ataagttata aagctagctt gagatattgt tgacgattac
     9361 ataattagtt cgttcgaata tctatttaat tttacaaatc tatacttaca agatatactg
     9421 aaagtaacaa atattaagtg catcaaaaca ccaatccccc aatctattgc agtagattgg
     9481 gggatctttt agtgttgact atgtcgtctt attttttatt acgttgttcc agccaatatt
     9541 taaacaacgt aacactgcac tctactataa taggtgctat tataaaaaca aatattaagt
     9601 acatcattac acctccttcc gtcgaatcta tcgccggaag taagacgact tcaatagtat
     9661 aacaaaaatt tttaatttaa aacggtgtgt tatcacgtga aattgtgaat aaagatacaa
     9721 aatatctata aatgaaaaag tatgaaaaca cgtgagaaca acgatgtata aaagatgaat
     9781 aaacgataat cataaggaaa aggcttaaac gctgttaaat caacgtttaa gcctttttgt
     9841 gtagttgaca taattaacta ttatgggtag tacttttgta tatactattc attttataaa
     9901 tgactattaa tcactgtaaa aaataataca aaagagccat tcttattaat aaaccattat
     9961 atgcttgttt agtaaatatc acattatcca tgttatctgt atctgaagaa agttcagctt
    10021 ctcttggtcc aggacttaga atagggattc tattcttatc attaaatttt tctttagata
    10081 ctatcaaatt attttctaat gatggttttt catcaacttt ttctgaataa gcggaattaa
    10141 aatctggcag ttcaaaaggt ataatatcta atatatcagc tagctctaat actctttcta
    10201 cattatgaac tatagagtaa tcagtatttt ttaataaatt tagaatatcg ctatttaatt
    10261 cttcgccatt aggtaataga aatacaattt tcttaatatc aaacttttct agacccataa
    10321 caatagaacg aatcactcta caattaatat ccccacctat tcctataact ttatctttta
    10381 aaccatttaa ttcttttgac atcgtatata aa
//
