LOCUS       NZ_CP093190            11435 bp    DNA     circular CON 20-JUL-2023
DEFINITION  Staphylococcus epidermidis strain TMDU-302 plasmid p2, complete
            sequence.
ACCESSION   NZ_CP093190
VERSION     NZ_CP093190.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26517680
            Assembly: GCF_024204825.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Saito,R., Ayibieke,A. and Yamada,K.
  TITLE     bloodstream S. epidermidis isolates from two Hospitals in Tokyo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   Saito,R., Ayibieke,A. and Yamada,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45 yushima, Bunkyo-ku, Tokyo 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembley v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 742.0x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/20/2023 03:47:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,450
            CDSs (total)                      :: 2,366
            Genes (coding)                    :: 2,299
            CDSs (with protein)               :: 2,299
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 34 of 67
            Pseudo Genes (incomplete)         :: 41 of 67
            Pseudo Genes (internal stop)      :: 22 of 67
            Pseudo Genes (multiple problems)  :: 24 of 67
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093190.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="TMDU-302"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p2"
                     /geo_loc_name="Japan"
                     /lat_lon="35.701735 N 139.7642509 E"
                     /collection_date="2015-07-11"
                     /collected_by="TMDU hospital staff"
     gene            join(11215..11435,1..643)
                     /locus_tag="MNU45_RS12055"
                     /old_locus_tag="MNU45_12050"
     CDS             join(11215..11435,1..643)
                     /locus_tag="MNU45_RS12055"
                     /old_locus_tag="MNU45_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456280.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_029376496.1"
                     /translation="MKISDITKKYKDNTVLDHLNFSFDSSHIVGLIGKNGVGKTTIMKI
                     MNGNIVNFKGKVDFDQNQKIGYLIEHPKLYQDLSGLKNLKNFSNILGVKFDKDYANKII
                     KAFDMESYINKRVKKYSLGMKQKLAIAVSLINKPHYLILDEPTNGMDPDGSIDVLNTIK
                     DITNEFNMKVLISSHKLEDIELICDRAVFLRDGKFVEDIDMKNTSSQEYTSITFNEIDV
                     EKAKNHLNSKSHGSIKKNGNILIIPKLDNYQNLLKDLANINIFPTKIEDKISTLRDTYF
                     NINKGE"
                     /name="ABC transporter ATP-binding protein"
     gene            752..1375
                     /locus_tag="MNU45_RS12060"
                     /old_locus_tag="MNU45_12055"
     CDS             752..1375
                     /locus_tag="MNU45_RS12060"
                     /old_locus_tag="MNU45_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016898832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_225975801.1"
                     /translation="MTQVHNNHKVSGETILTLSTWLFAFWGVMIITSFLVRDFSQGTSQ
                     LYLNSFKNRAKYFISQLVSILIASVIVFLTLYVFVLIMQNIGDGQPIKNEVVGKTLGIY
                     ILLFLFYGLFLFLITLLVKSSSLVFSIGVFLILIIPIATNLVPLIPEYGDEVKKALKYI
                     PFNFLIDNVWLGSLKLNGWQTFISIASVILLAIIDFFTISKRDY"
                     /name="ABC transporter permease"
     gene            1598..1969
                     /locus_tag="MNU45_RS12065"
                     /old_locus_tag="MNU45_12060"
     CDS             1598..1969
                     /locus_tag="MNU45_RS12065"
                     /old_locus_tag="MNU45_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020368225.1"
                     /translation="MKKLSPSGKFLTVFASALYIISAILTGAFFGSDFSNVMLPITVYI
                     LFTLLITHRWEGYEEKRLLDLNNIALLSYGVSYILFLWLSKFTEDKVYLGAIFLGSLLV
                     FIFIFIFALRSLTKKNWWK"
                     /name="hypothetical protein"
     gene            2093..2542
                     /locus_tag="MNU45_RS12070"
                     /old_locus_tag="MNU45_12065"
     CDS             2093..2542
                     /locus_tag="MNU45_RS12070"
                     /old_locus_tag="MNU45_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="WP_020368226.1"
                     /translation="MILISACLIGQNVRYDGGNKINNNLKKLVDQGIAKPICPEILGGL
                     PTPRNPAEILGGDGFDVLENKAKIIDNHGKDVTKEYVIGAMKALDTCKKMNCKTLILKS
                     DSPTCSTENIYTGEFNGNKKQGVGIFSALLIKNGIQVYNEKNYKI"
                     /name="DUF523 domain-containing protein"
     gene            complement(2975..3157)
                     /locus_tag="MNU45_RS12075"
                     /old_locus_tag="MNU45_12070"
     CDS             complement(2975..3157)
                     /locus_tag="MNU45_RS12075"
                     /old_locus_tag="MNU45_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020368196.1"
                     /translation="MKVVLYFVYFFVSFFILNLLINLVIPGIDVDVCESLISALFFAIF
                     MTPVLYVLRGRRNKK"
                     /name="hypothetical protein"
     gene            complement(3200..3487)
                     /locus_tag="MNU45_RS12080"
                     /old_locus_tag="MNU45_12075"
     CDS             complement(3200..3487)
                     /locus_tag="MNU45_RS12080"
                     /old_locus_tag="MNU45_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="WP_020368197.1"
                     /translation="MKKIHENYKKRIKFLYFTLGTIMLVFMFFALVMTMGTDILSFFNV
                     ETLKKVIASKIKASEGFMTFVWYIAYGLPYIFAIGLFIDFYEWIRGRFHD"
                     /name="DUF334 domain-containing protein"
     gene            complement(3601..>3696)
                     /locus_tag="MNU45_RS12085"
                     /old_locus_tag="MNU45_12080"
                     /pseudo=""
     CDS             complement(3601..>3696)
                     /locus_tag="MNU45_RS12085"
                     /old_locus_tag="MNU45_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3820..4173
                     /locus_tag="MNU45_RS12090"
                     /old_locus_tag="MNU45_12085"
     CDS             3820..4173
                     /locus_tag="MNU45_RS12090"
                     /old_locus_tag="MNU45_12085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_275584108.1"
                     /translation="MNETYMLSRIDKIIVHSFGLFVNFMFINFIQLVNVCFFDNYSLTL
                     AYLFFFSTMVWNLIPILNSDGYKIMLAALSLDEFNNFTKNHWIILISQIIGITIALNTL
                     IHWIIYWSKYFYP"
                     /name="hypothetical protein"
     gene            4186..4821
                     /locus_tag="MNU45_RS12095"
                     /old_locus_tag="MNU45_12090"
     CDS             4186..4821
                     /locus_tag="MNU45_RS12095"
                     /old_locus_tag="MNU45_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191156.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_002498748.1"
                     /translation="MKIDNYSLKIKRKNLIDNCDLNFYPGYVNHIVGKNGVGKSQLAKD
                     FILNNSGNIPKELSNKVTLISSSSNVPKDITKNALLELLQDKFKNSSQIFSEINEILNI
                     KEIPSNILIKNLSDGQKQKLKFLSFLLEDNSLIILDEVTNALDKKTVNDIYAFLNKFIQ
                     NNTSKTIVNITHNLSDLNSIPGKYFIFRNYQIEEYSSKDQLVNDYINL"
                     /name="ATP-binding cassette domain-containing protein"
     gene            4838..5560
                     /locus_tag="MNU45_RS12100"
                     /old_locus_tag="MNU45_12095"
     CDS             4838..5560
                     /locus_tag="MNU45_RS12100"
                     /old_locus_tag="MNU45_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="WP_029376494.1"
                     /translation="MKLEFKKSISNKILLTLGILFIFLFLLGYFLPVGIDKAKHLSYSQ
                     YFFSAYTVATEFGFLLFSFIIAYFINKEYSNKNTLFYKLIGENIFSFFYKKALVLFVEC
                     FIFIFLGITIISLVFSNFSHYLLLLILFSLVILQYILIVGTISLISPNVLVSIGISIIY
                     WIGSIILVAINKNVFGVIAPFEASNSMYMSIEKILNNKMNMINSHDVWTIILFFFIIFI
                     VNFIILISSKSRWLKLGM"
                     /name="peptide ABC transporter permease"
     gene            complement(6058..6477)
                     /locus_tag="MNU45_RS12105"
                     /old_locus_tag="MNU45_12100"
     CDS             complement(6058..6477)
                     /locus_tag="MNU45_RS12105"
                     /old_locus_tag="MNU45_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002511307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002440654.1"
                     /translation="MPKKQNSKEKILTIRVSQSQYEKICALADIREMNVTEYIRHTAIG
                     NRIKPTIIEQIKESSEVSELFKKDNEALKSNNQALQHHIHQLEDEIDQLRQENDVFHHL
                     LQHFDSTAFLNFNTYRDDRPLKNAIKRLKEQYKSK"
                     /name="hypothetical protein"
     gene            6932..8140
                     /gene="mobV"
                     /locus_tag="MNU45_RS12110"
                     /old_locus_tag="MNU45_12105"
     CDS             6932..8140
                     /gene="mobV"
                     /locus_tag="MNU45_RS12110"
                     /old_locus_tag="MNU45_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_254106769.1"
                     /translation="MKLAWNTQKNKMSDVKGKEMEQERKGRIKNDEIDLNRTNLNFDLV
                     EDERHLYHRVKDRIEYYKKQGSRVQKNSVVMYSNIITLSKEEADRMGETRTKHYFKTCK
                     DYFSGRFGEANVVSAKVHMDESAPHMHLHFIPVNHQGRLSARTAMNRQAINHIHDELTT
                     HLCQQGFDVERGSTDDNKTYIQDIHEYKKKAKVLSELDQQIETKKQLLRDINDLHDEKE
                     DLTRNIQNLKRKNHFLQEDSQQVQEDIDLYKHLRAISRDDFKKEEEKYQKEIKAYEASL
                     SGLRMNYRDLDKRNQHLTTQNNQMEHHLDMLNTRHQAMNDMVRNDTRILFAGLRCALKD
                     MNIELSAEIKYQRMPEVGIDPDTVDQGALTHAYQGDVSGIDHHIRKASAPIKKETENDL
                     ELE"
                     /name="mobV"
     gene            8814..9680
                     /locus_tag="MNU45_RS12115"
                     /old_locus_tag="MNU45_12110"
     CDS             8814..9680
                     /locus_tag="MNU45_RS12115"
                     /old_locus_tag="MNU45_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494364.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_049368611.1"
                     /translation="MSNETVIYHNEMNLVPLRKFTSVEIDLFFAMCNKLKEQDTNKLYL
                     SFEELKYLSNYNYETRNINRFIGDLQNIYKKMLEITYRREDEDVIEFFVLFNHFKIHKT
                     EKYLEISTNPDLKFVLNDITRNFTKFELKELTNLKSSYSKTMFRLLKQYKHTGYLKLSM
                     EDFRTRLDVSKSYQMCDITKRVLKPISKELTPIFNNLNINKVKAKKGRKIEYLEFIFDA
                     EKRIHNKRQPQITNIGKSRQYTNREKTPKWLDDKIYKQSQELHDEDAKLKQDREAFQRQ
                     LEEKWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            9786..9929
                     /locus_tag="MNU45_RS12120"
                     /old_locus_tag="MNU45_12115"
     CDS             9786..9929
                     /locus_tag="MNU45_RS12120"
                     /old_locus_tag="MNU45_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002485548.1"
                     /translation="MSNRYNIHPALYIGGFAVFFSFIFGFPVGTLMGLAIGIGISKKNE
                     NL"
                     /name="hypothetical protein"
     gene            complement(10795..11133)
                     /locus_tag="MNU45_RS12125"
                     /old_locus_tag="MNU45_12120"
     CDS             complement(10795..11133)
                     /locus_tag="MNU45_RS12125"
                     /old_locus_tag="MNU45_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002452560.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_029376497.1"
                     /translation="MKDLINCENKPMYLNISENIIQNIYEENYKTQKLPSIRKSAEIFD
                     VSIITIKSAYQLLEKLEIIIANPKIGYIISDNAKDKILAIKKEELKQLIFKAEAISNKY
                     NISFFDLM"
                     /name="GntR family transcriptional regulator"
     Typing          6861..8137
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.775"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atatcaagac ctatcgggat taaaaaactt aaaaaatttt agtaatatat taggtgtaaa
       61 gtttgataaa gactatgcta ataaaattat aaaagcattt gacatggaaa gttatattaa
      121 caaaagagta aaaaaatatt ctttaggaat gaaacaaaaa ctagcaatcg ctgtgtcttt
      181 aataaacaaa ccacattact taatacttga tgagcctacg aacggtatgg atccagatgg
      241 ttcaatagat gtattgaata ctattaaaga tataactaac gaattcaaca tgaaagtatt
      301 aatatcaagc cataaattag aagatataga actcatatgt gatagagcag tatttttacg
      361 cgatggaaag tttgttgaag atattgatat gaaaaataca tcatcacaag agtatacatc
      421 tatcacgttt aatgaaattg atgtggaaaa agcaaaaaat catttaaatt caaagtcaca
      481 tggttcgatc aagaaaaatg gaaacatctt gataattcca aagctcgata attatcaaaa
      541 tttactaaaa gatttagcta atattaatat atttccgact aaaattgaag ataaaataag
      601 tacacttagg gatacttact ttaatattaa taaaggagaa taaacaatga gtatatatca
      661 tttaacaaaa tttaatatat caagattgct aaaaaatcct ttcaattata ttgggttact
      721 atttgtttta ataatcccca tattttatac aatgactcag gttcataata atcataaagt
      781 ttctggagaa actattttaa ctctttcaac atggctattt gcattttggg gagtgatgat
      841 tattactagc tttttagtca gagacttttc tcagggtaca tcacaattgt atttgaatag
      901 ttttaaaaat agagcgaaat actttatatc tcaactggta tccatcttaa ttgctagtgt
      961 catagtattt ctaacattat atgtattcgt tttaataatg cagaatattg gcgatggtca
     1021 acctataaaa aatgaagtgg ttggaaaaac tttaggaata tacatcctat tatttttgtt
     1081 ctatggtcta tttttatttc taattacttt attagtaaaa agttcttctt tagtgttttc
     1141 aattggtgtt tttcttatat taatcatacc tatagcaacc aatttagttc cattaattcc
     1201 agaatatggt gatgaagtta aaaaagcctt aaaatatata ccttttaact ttttaattga
     1261 taatgtttgg cttggatctc tcaaattaaa tggatggcaa acatttatct caatagcttc
     1321 agtaatatta ctagctatca ttgacttctt cactatttct aaaagagatt attaaaaagt
     1381 aacaattcta tataaacata taagaaaagc ttaaacgttg tttaaaaacg tttaagcttt
     1441 tcttacttaa ttaacataaa ttacgattat aggtagttga aatgtatatc ttatacattt
     1501 tcctttttta attactccca tgatgcaact tctaaaaagg ttcttaagaa atagctgtaa
     1561 taaatgttat atttaaactt aaggaggaga tgatattatg aaaaaacttt caccatcagg
     1621 aaaattttta actgtttttg cttcagccct atatatcatc tctgcaattc ttactggggc
     1681 tttttttggg agtgattttt ccaatgtaat gttaccaata acggtataca tactattcac
     1741 tttattaatt acacaccgtt gggaaggtta tgaagaaaag cgtttgcttg atttaaacaa
     1801 tatagcttta ttaagttatg gagtttctta tatcctattc ttatggttat ctaaatttac
     1861 ggaagataag gtttatttag gagcaatttt tttgggatct ttactagttt ttatatttat
     1921 ttttatattt gcgttacgtt ctcttaccaa aaaaaattgg tggaaataaa aagcattcct
     1981 taattattag tgttttggaa gtaagtgtga aataccaaat caaaagttaa tcaagataag
     2041 aagaaactcg tttttaactc gtttccaaaa gctaaaatcc ggaggataaa gtatgattct
     2101 tataagtgca tgtttaatcg gtcaaaatgt tagatacgac ggaggaaaca aaatcaacaa
     2161 caatttaaaa aaacttgtag accaaggtat agcaaaacca atatgccctg aaattttagg
     2221 aggactacca actccaagaa atcctgcaga gatcttagga ggagacggct ttgatgtatt
     2281 ggaaaataaa gctaaaatta ttgataatca tggaaaagac gttacaaaag aatatgtaat
     2341 tggagctatg aaagcattag atacttgcaa aaaaatgaat tgcaaaacat taatcctaaa
     2401 atctgatagt ccaacttgca gcacagaaaa tatttacact ggtgaattta acggaaacaa
     2461 aaaacaagga gtaggaatat tttcagcact actaataaaa aacggaatac aagtttacaa
     2521 cgaaaaaaat tataagattt aaatacttta ttttgtacac atatcttaat cgaataatat
     2581 tatcactatt ttccagctac tcatttctga ctagtctttt attctgcgtt ttaagaggtc
     2641 actttaaaag atcttactat caaatgattt tggatgaggt ataaataaat gttaaagtag
     2701 aaacataaaa ttattcccca ttcagcattg atttttaaca taaagtaatt ctctaactaa
     2761 acccatagcc actcagtttg ttgagtggct attttaatgc tttattccaa ttgatttatt
     2821 gacgttgagc ctctctctga actctgaacc cttaacaatc cctaaacttg tcgaatcgtc
     2881 ggcttaatag ctcacgctat gccgacattc gtcttcaagt ttagttaagg gtccttctca
     2941 acatcaataa attttctcgg cataaatgct cgttctattt tttgtttctt cttcctctta
     3001 aaacataaag aactggcgtc ataaatattg caaaaaacaa tgcagagata agactctcac
     3061 atacatcaac atcaattcct ggtatcacta aattaattag caaatttaaa ataaaaaaag
     3121 atacaaaaaa atacacgaaa tataaaacaa ctttcaaaat ttggcctcct tttccaacaa
     3181 aactttatca cattcctctt taatcatgga accgtcctct aatccattca taaaagtcaa
     3241 taaatagtcc tatagcgaat atataaggta aaccatatgc aatataccaa acaaatgtca
     3301 taaagccttc agatgcttta attttgcttg ctatgacttt cttaagcgtc tccacgttga
     3361 aaaaagataa tatatccgtt cccattgtca tgactagtgc aaaaaacata aacactagca
     3421 tgattgttcc taacgtaaaa tataaaaact taattcgctt tttataattc tcatgtattt
     3481 tcttcatttc ttcccagcaa ttatgatgtt tatagtatca acaaaaaaga aaaaagagca
     3541 aaaataattc aataagaaaa ggacaggtca acctacgtaa aaggtttctc tgtccttttc
     3601 ttattgccaa aattctattt gcttttgtaa ttgtttttca ttttctctta aagtcatctt
     3661 cattaattca gttaaatcga ctggagatat ttctttaaat ctattaatat tttattacat
     3721 gagttaggtc atatatatgc tttaaaattt ttcagaagga aaattgataa agttggcttc
     3781 agattaaact tttatgtttt cccatctttt tacgttcaaa tgaacgaaac gtatatgcta
     3841 tctagaatag ataaaatcat agtacattct ttcggtttat ttgttaattt tatgtttatt
     3901 aactttattc aattagttaa tgtatgtttc tttgataact attctttaac tttagcgtat
     3961 ttatttttct tctcaaccat ggtatggaat ttaataccta ttttaaattc agatggttat
     4021 aaaataatgt tagctgcatt atccttagat gaattcaata atttcactaa aaaccactgg
     4081 ataattctaa tatctcaaat cattggtatt acaattgctt taaatacact aatacattgg
     4141 atcatttatt ggagtaaata tttttatcca taggagctgt aaacattgaa aatagataat
     4201 tattctttaa aaataaagcg aaaaaattta attgataatt gtgatttaaa tttctaccct
     4261 ggctatgtta accatatagt tggtaaaaat ggtgtaggaa aatcacaatt agcgaaagat
     4321 tttatattaa acaatagcgg taacattccc aaagaacttt caaataaagt aactttaatt
     4381 tctagttctt ctaatgtgcc gaaagatata acaaaaaacg ctctattaga attgttacaa
     4441 gacaaattta aaaattcctc tcaaatcttt tctgaaatca atgaaatact taacattaag
     4501 gaaataccat ctaacatatt aattaaaaat ctaagtgatg gtcaaaaaca aaaacttaaa
     4561 tttttaagtt ttttattaga agataattca ctaattatat tagatgaagt aaccaacgct
     4621 ttggacaaaa aaacagtcaa cgatatttat gcttttttaa ataaatttat ccagaataac
     4681 acttctaaaa ctatagttaa tattactcat aatttatcag atttaaatag cataccagga
     4741 aaatatttta ttttcagaaa ttaccaaata gaagaatatt catctaaaga tcaattagta
     4801 aatgactata ttaatttata aacaaggagt gtaagctttg aaattagaat ttaagaagag
     4861 tatctcgaat aaaatattat taactttagg aatcttgttt atttttcttt ttttattagg
     4921 ttatttttta cccgttggaa ttgataaagc taaacatttg agttacagtc aatacttttt
     4981 tagcgcttat actgttgcaa cagaattcgg ctttttatta ttctcattta tcattgccta
     5041 tttcataaat aaagaatact caaataagaa tacacttttc tacaaactta taggagaaaa
     5101 tatcttttca tttttttata aaaaagcgtt ggttttattt gttgaatgtt ttatttttat
     5161 ctttttagga ataactatta tttctttagt tttctctaat ttttctcatt atctgttatt
     5221 attaatttta ttctcattag ttattttaca atatatatta attgttggaa ctataagtct
     5281 catttcacct aatgttttgg taagcattgg tataagcatt atttattgga ttggctctat
     5341 aatattagta gctatcaata aaaatgtttt tggcgttatc gctccttttg aagccagcaa
     5401 ctctatgtat atgtctatag aaaaaattct taataataaa atgaatatga ttaactcaca
     5461 tgatgtgtgg acaataattt tattcttttt cattattttc atcgtcaact tcattatatt
     5521 aatttcatct aaaagtagat ggttgaaact aggtatgtag agttatcttt ttatttaaat
     5581 atctagcgat taaatattac tttttatcat agaaaatcta ttacaaagta gattttaaag
     5641 tgtaattaga aacagtaaaa gagactagcc aaatcgctag tatcttttac tgtttctaat
     5701 ttgattaagc aataaccgat catttttgta actcccaaaa ttaatatatc ctgtactatc
     5761 gatatgcgtt aacatacgtt tgaatgtgtc tacttgaggt tgataatgtt tgactgtttg
     5821 ctctaattct tctatatgat gtgattgagc ttgtataagc tactcatatt cattgatttt
     5881 gctttctaat tcactaacct ttgaatcctc ttcataatct tctaatgtcg tttgttcttt
     5941 tggatattca atgaccgtcg gtttaatacg attacccaca gcttttatta aaattattac
     6001 accataagtc agaagctact taatataata aaagcaagtg atttataaaa ttataaatca
     6061 cttgctttta tactgttctt ttaatcgttt aatcgcattt tttagtggac gatcatcacg
     6121 atatgtattg aagtttaaaa atgctgtact atcaaaatgt tgtaataagt gatgaaagac
     6181 gtcattttct tgtctcagtt ggtcgatttc atcttccaac tgatgaatat gatgctggag
     6241 tgcctgattg ttcgatttga gtgcctcgtt gtctttttta aacaattcac tgacttcact
     6301 ggattcttta atttgttcga tgattgtcgg tttaatgcga ttaccaatgg ctgtatgacg
     6361 tatgtattcc gtcacattca tctctcgaat atccgcaagt gcacaaatct tttcgtattg
     6421 cgattgactt acacgaattg ttaagatttt ttcttttgaa ttttgttttt ttggcattga
     6481 accacctcat cgttaatata tgataagcat acatatgtgt gcttatcttt atattaatat
     6541 aacatacata tgtatgctaa taaatatgta tgattcgagg tggtatgagc taacatttgg
     6601 catacagatt ttcaaacata tttttttgat aatgcgtatg tcatttgtta gctatagaac
     6661 ggaattcaaa aatatatttt tgaatgtagt gatggcctcg ccagagcgaa gataactctt
     6721 acatttgaat gtaagagtat atcgagctat actctcattg cggaatcagc ctcacatgtt
     6781 tgaggcgtag cggtgagagt atccatcatt ttacataagc gaagcattgt cgaaggtaga
     6841 caatcacagc gcttgtaaaa tgatatttgc gaaggttctc gaaagtagag aaccacagac
     6901 aaatcaaaaa gcttcgggga gggcgctcaa tttgaaatta gcttggaata cgcaaaaaaa
     6961 taaaatgagt gatgttaaag gcaaagagat ggaacaagaa cgtaaaggga gaatcaaaaa
     7021 cgatgaaatt gacttgaacc ggacaaactt aaactttgat ttagttgaag acgaacgtca
     7081 tttgtaccat cgtgtgaaag atagaattga gtactataaa aaacagggct cacgtgtaca
     7141 gaaaaatagt gtggtgatgt attccaatat cattacgtta tcgaaagaag aggccgaccg
     7201 tatgggagag acgcgtacaa agcactactt taaaacatgt aaagattact tcagtggacg
     7261 ttttggcgaa gccaatgtcg tttcagcgaa agttcatatg gatgaatcag caccccatat
     7321 gcaccttcac tttatacctg ttaatcatca agggcgatta tcagcacgta cagccatgaa
     7381 tcgacaagcg attaatcaca tccatgatga attaacaaca catctgtgcc agcaaggttt
     7441 tgatgttgaa cgtgggagta ccgatgataa taaaacgtat attcaagata ttcatgaata
     7501 caaaaagaaa gcgaaagttt taagtgaatt agaccaacaa attgaaacga aaaaacaatt
     7561 gttgagggat atcaacgatt tacatgatga aaaagaagat ttaacgcgaa atattcaaaa
     7621 cttaaaacgg aaaaatcatt ttttacaaga ggattctcaa caagttcaag aagatatcga
     7681 cctatataag catctgagag cgatttctag ggacgatttt aaaaaagagg aggaaaaata
     7741 tcaaaaagaa atcaaagcgt atgaggcgtc tctgagtggt cttagaatga actaccgaga
     7801 tttggacaag cgcaatcaac atttaacaac acaaaataat cagatggaac atcatttaga
     7861 catgttaaat acgcgtcatc aagcgatgaa cgatatggta agaaacgata cacgtatttt
     7921 gtttgcgggg cttcgatgtg cattgaaaga tatgaacatt gaattaagcg cagaaattaa
     7981 atatcaacgc atgccagaag tcggtattga tcccgatacg gtcgatcagg gggctttaac
     8041 acatgcatat caaggtgatg tgtcagggat agaccatcat ataagaaagg catcagcacc
     8101 tattaaaaaa gagacagaga atgatttaga attagaataa atcaataaaa taagcactta
     8161 aacaagtata atactcgctt aagtgcttat ctcatgaaaa gaggagataa aaatttaata
     8221 agtatgatca acctattaag aacaatcatt accttactta cagcgcttat taaatagttc
     8281 tgtaaggagg aacaatacct ttgcatttct atattattat aagaattcca aggtagcaaa
     8341 gatattgtat tggaagtgat agatgtgagt atgatttcta aattaattga tgaagatatt
     8401 tggggtttct aaactttaaa aaatacctcc attcttttaa aagtttattt ttgtgttggt
     8461 ttctttagaa atatatttga atgcggaatg cgtttgaagt attttactcc gaccttttta
     8521 atatattttt ataaaccttt ttataaacct ttttaggtgt gttttaatcc ttagagcgac
     8581 agtggatttc acaacttaaa aaggtcattt tgccacataa aaaaggtcat tttgccacat
     8641 aaaaaaggtc attttgccac ataaaaaagg tcattttgcc acataaaaaa ggtcattatt
     8701 ttactccgac cttttttatg tggcaaatac ttttaataaa gctattatag tgataatatg
     8761 tatatgatat acaaggactt ataaaaataa cgaccttttt gggagtgata tatatgtcga
     8821 acgaaactgt tatttatcat aatgaaatga atttggttcc tttgagaaaa tttacttctg
     8881 ttgaaataga tttatttttt gctatgtgta ataagctaaa agaacaagat actaataaat
     8941 tatatttaag ttttgaagaa cttaaatatt taagtaatta taactatgaa actagaaata
     9001 ttaatcgttt tattggagat ttacaaaata tttataaaaa gatgctggag ataacttatc
     9061 gaagagaaga tgaggatgta atagagtttt ttgtactttt caatcacttt aaaattcata
     9121 aaacggagaa gtatcttgaa atttccacta atcctgactt aaagtttgtt ttaaatgata
     9181 ttactcgtaa ttttacaaaa ttcgaattaa aagaactgac taatttaaaa tcttcgtact
     9241 ctaaaaccat gtttcgctta cttaaacaat ataaacatac tggatattta aaattatcaa
     9301 tggaagattt tagaactcga ttagacgtat caaagtctta tcaaatgtgt gacattacta
     9361 aacgtgtatt aaagccaata tctaaagagc ttacacctat ttttaataat cttaatatta
     9421 ataaagttaa agctaaaaaa ggtcgcaaaa ttgaatactt agaatttatc tttgacgctg
     9481 agaaacgcat tcacaacaaa cgacaaccac aaataactaa tataggtaag tcgcgccaat
     9541 atactaatcg tgaaaagaca cctaaatggt tagacgacaa gatatataaa caatctcaag
     9601 agttacatga cgaagatgca aaattaaaac aagatcgaga ggcatttcaa cgtcaattag
     9661 aagaaaaatg ggaggaataa taatttaaat gagctacata tatattttat tcttatttcc
     9721 attaatagtg ggatttatcg tatcaataaa aaagaaaaaa catgactaag gagagataaa
     9781 aatttatgag taatagatat aatatacatc cagcattata tataggcgga tttgcggtat
     9841 tttttagttt tatatttgga tttcctgtag gaacattaat gggactagct ataggaatag
     9901 gaattagtaa aaaaaatgaa aatttgtaaa agagtttaca gatgaagaag ttacacgata
     9961 tactgtaatt aacaaattgt aggtgtacat cactgtacgc aaagcccccc acagccggca
    10021 agctacgtgg ggggcttttt actatcgttg tttcaaacta tcataaaaat agtttgccat
    10081 aatactaatt atgagtggat caacgatatt taagataaca aattgtagga tatccaacac
    10141 tatacgcaca cctcctttct gcacaggaag tgcactatct attatatata tcgaatatca
    10201 aagacaaaag agtcactgta taaatataaa aaaaatcatt ttatgaaaac ttatgaaaac
    10261 acgtcgttaa agggatgtat aaaagttgta taaagaggaa tgatgacaaa aagggacaaa
    10321 cgccgtcatt tcagcgtttg tcccttttga gttagttgac ataataaagt gttataggaa
    10381 cttcaaacga atattttatg cattatcaaa aataaatgaa ataaacttta aaagaaaata
    10441 aaggataatt gcataaacaa attgattttt ggcaaagtga tgttaccact taaattatct
    10501 ataaaaaagg acaaatgcta atatgaaagc atttgtcctt ttttatatcc gaacataatt
    10561 acataactat tggtttgaaa tgaacaatta atattattta attattttcc tgagttgaat
    10621 gcgcctttca taaatccttt atatgcttca acaagacctt ttcctaaatc catcgttaca
    10681 ccaccttttt tatatgaaag ttactatttc atatatgtag tacaaatgca gaataacatt
    10741 ccaattattt caaatcaatt ttctattata attttaaagt taacttaaac atttttacat
    10801 taaatcaaaa aaagatatat tatatttatt agatattgct tctgctttaa aaataagctg
    10861 ttttaattct tcttttttta ttgctaatat cttatcttta gcattatcac taattatata
    10921 tccaattttg ggattagcta ttataatttc gagtttttct aaaagttggt aagctgattt
    10981 tatagtaata atgcttacat caaatatttc ggctgatttt cgaatggaag gaagtttttg
    11041 ggttttataa ttttcttcat agatgttttg tattatattt tctgatatat ttagatacat
    11101 aggtttgttt tcacaattaa ttaaatcttt catataaacc ctactttcac ttattttttt
    11161 aaatcaatat tttatttctt atatttttat aaaaattata aaaaggagag cacaatgaaa
    11221 atatcagata ttactaagaa gtacaaagat aatacggtat tagatcattt aaatttttca
    11281 tttgattcta gccatatcgt tggattaatt gggaaaaatg gagtcggaaa aactaccatt
    11341 atgaaaatta tgaacggcaa tattgtgaac tttaaaggca aagttgattt tgaccaaaat
    11401 caaaaaattg gttatttaat tgaacatcct aagct
//
