LOCUS       NZ_CP093176             8533 bp    DNA     circular CON 20-JUL-2023
DEFINITION  Staphylococcus epidermidis strain TMDU-137 plasmid p3, complete
            sequence.
ACCESSION   NZ_CP093176
VERSION     NZ_CP093176.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26515364
            Assembly: GCF_024204745.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Saito,R., Ayibieke,A. and Yamada,K.
  TITLE     bloodstream S. epidermidis isolates from two Hospitals in Tokyo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Saito,R., Ayibieke,A. and Yamada,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45 yushima, Bunkyo-ku, Tokyo 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembley v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 249.0x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/20/2023 03:41:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,479
            CDSs (total)                      :: 2,395
            Genes (coding)                    :: 2,333
            CDSs (with protein)               :: 2,333
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 37 of 62
            Pseudo Genes (incomplete)         :: 33 of 62
            Pseudo Genes (internal stop)      :: 22 of 62
            Pseudo Genes (multiple problems)  :: 24 of 62
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093176.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="TMDU-137"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p3"
                     /geo_loc_name="Japan"
                     /lat_lon="35.701735 N 139.7642509 E"
                     /collection_date="2015-03-09"
                     /collected_by="TMDU hospital staff"
     gene            complement(216..1142)
                     /locus_tag="MNU44_RS12300"
                     /old_locus_tag="MNU44_12290"
     CDS             complement(216..1142)
                     /locus_tag="MNU44_RS12300"
                     /old_locus_tag="MNU44_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020365845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="WP_049324113.1"
                     /translation="MINNLLSMENLNLNDVNSLFEKADYYEKALKDKKVIKDLEGKILA
                     TLFYQPSTRTRMSFTSAMYRLGGQVIDMGGPKVARTDDYFDESMSDTIKYIEQYSDIMV
                     MRHFQNYAVEKASKMTHIPMINAGDGTNEHPTQALLDLYTMSKELKGLEDKIIGVGGDI
                     NCRVIRSIVIGLEKFSIKKIIFLLPYGEELNNDILSLLKNTDYSIVYSVEKVLEQADIL
                     DIIPFDLPDFNSSYSKKVNEKPSLENNLIVSKEKFNDKNKIPILSPGPREAELSPDTDN
                     MENVIFTKQAYNGLLIRMALLYYFLHR"
                     /name="aspartate carbamoyltransferase"
     gene            complement(1135..2025)
                     /locus_tag="MNU44_RS12305"
                     /old_locus_tag="MNU44_12295"
     CDS             complement(1135..2025)
                     /locus_tag="MNU44_RS12305"
                     /old_locus_tag="MNU44_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486791.1"
                     /GO_function="GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="WP_024053338.1"
                     /translation="MNSKVIKYTADFNEKRYWERIKRNLGWLGDTDEEAKKRQKKISEV
                     VIGIAGCGGIGGAVAERLVRLGVRHIKVADPDHFELSNINRQFGASLDNLGRNKAEVVG
                     ESIFNISKDVNVDVYPEGINNSTADDFVEGCDYVLDKIELFELEARYALHKAFKKSERC
                     KFMLTVPVFGHRAFFFKWTKDSMSAEDYFGIKPGSELDEQNTRKIVYSLFPEYPQFPSK
                     ERLDDWLIYNKECPIFAGCPPLAQGLLVERLSFEIADLKDIPEYKELPSTPSYSMFDSA
                     SWETKIVEREENIID"
                     /name="ThiF family adenylyltransferase"
     gene            complement(2144..2842)
                     /locus_tag="MNU44_RS12310"
                     /old_locus_tag="MNU44_12300"
     CDS             complement(2144..2842)
                     /locus_tag="MNU44_RS12310"
                     /old_locus_tag="MNU44_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020365681.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_145352373.1"
                     /translation="MYEKFADNILLIGHKTRLTMLIELSSGVSIPAGELAKLANVTPQT
                     ASEHLSKLVKANLISVNKRGRHCYYKIDNSEIVDAINALSIISPPMSSKSLKETTKKEK
                     LSYMRTCYGHIAGKIGVKFTEALLENDYLRESKDCYKLTQKGKFWLKKIGIATEKAMYS
                     KPIPKHIDWTERKNHIAGPIALQITEKLIELSWVKKSKFNRCLNLTLKGKKAFKTYLNM
                     NITELENEIF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            2954..4333
                     /locus_tag="MNU44_RS12315"
                     /old_locus_tag="MNU44_12305"
     CDS             2954..4333
                     /locus_tag="MNU44_RS12315"
                     /old_locus_tag="MNU44_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_002502312.1"
                     /translation="MNSSKYFDHLVVFTAGIGMFLSTLDSGIINVALPTLSKSFNVDAS
                     FISWSITLYTLLLTGTIIIFGRLSDKYNRLNIYSIGLTVFLISSILCGFSNNVVELITF
                     RGLQGIGAAMLQGTATAIITTTIPEHRQGPALGTLSILLGIGPVLGPSIGGLLISIGNW
                     RWIFWINIPFIFIGLIGCLLLKKYIKEPKSVSIHLDIRGNSLLFVSIFCLLVSLTSWSH
                     HSFFNISVYSNFLIFIVTFCLFIVWELKTNHPIINLRLFKNISFSSSIFAIFVFGGTTS
                     LGFIIPPYILEKINHLSSWQIGLVNLTSPLGLVLISKITGKLISRVGNTILMTVGLVIM
                     IASYTSLGLLQYILNPITISLLLLIYGIGGGFFLPSNTSAIMGTVSKDMQGTVGATQRM
                     VQNIGIAVYTAITSLFISNQSQTNQLITGASHAWLFASATLFLSLLPFLLKLLHHKSEA
                     T"
                     /name="MFS transporter"
     gene            complement(4368..4787)
                     /locus_tag="MNU44_RS12320"
                     /old_locus_tag="MNU44_12310"
     CDS             complement(4368..4787)
                     /locus_tag="MNU44_RS12320"
                     /old_locus_tag="MNU44_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002511307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002502136.1"
                     /translation="MPKKQNSKEKILTIRVSQSQYEKICALADIREMNVTEYIRHTAIG
                     NRIKPTVIEQIKESSEVSELFKKDNEALKSNNQALQHHIHQLEDEIDQLRQENDVFHHL
                     LQHFDSTAFLNFNTYRDDRPLKNAIKRLKEQYKSK"
                     /name="hypothetical protein"
     gene            5242..6450
                     /gene="mobV"
                     /locus_tag="MNU44_RS12325"
                     /old_locus_tag="MNU44_12315"
     CDS             5242..6450
                     /gene="mobV"
                     /locus_tag="MNU44_RS12325"
                     /old_locus_tag="MNU44_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_254105787.1"
                     /translation="MKLAWNTQKNKMSDVKGKEMEQERKGRIKNDEIDLKRTNLNFDLV
                     EDERHLYHRVKDRVDYYKEQGSRVQKNSVVMYSNIITLSKEEADRMGETRTKHYFKTCK
                     DYFSERFGEANIVSAKVHMDESAPHMHLHFIPVNHQGRLSARTAMNRQAINHIHDELTT
                     HLCQQGFDVERGSTDDNKTYIQDIHEYKKKAKVLSELDQQIETKKQLLRDINDLHDEKE
                     HLTRDIQNLKRKNHFLQEDSQQAQEDIDLYKQLRANSRDDFEKDEEKYQKEIKAYEASL
                     SGLRMNYRDLDKRNQHLTTQNNQLEHHLDMLNARHQAMNDMVRNDTRILFAGLRCALKD
                     RNIELSAEIKYQRMPEVGIDPDTVDQGALTHAYQGDVSGIDHHIRKASSLIKKETENDL
                     ELE"
                     /name="mobV"
     gene            7124..7984
                     /locus_tag="MNU44_RS12330"
                     /old_locus_tag="MNU44_12320"
     CDS             7124..7984
                     /locus_tag="MNU44_RS12330"
                     /old_locus_tag="MNU44_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829553.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_254105789.1"
                     /translation="MSGETVVYRNEMNLVPLRRFTSTEVDLFFTLCNKLKEQDTRKVII
                     PFEELKYLSNYYTRSQERFINDLEHVYDKMLNLTYVERNGRSFEKFVLFTSYKVDLDEE
                     CLSISVNPDLKHILNSITADFTKFELKEMTHLKSTYAKNMFRLLKQYKHTGYIKINISD
                     FKNRLDIPKAYQMNDITKRVLKPISKELTPIFNNLNINKVKAKKGRKIEYLEFIFDAEK
                     RIHNKRQPQITNIGKSRQYTNREKTPEWLNEGRLGQSQEIHNEDAKLKQDREAFQRQLE
                     EKWED"
                     /name="RepB family plasmid replication initiator protein"
     gene            8089..8232
                     /locus_tag="MNU44_RS12335"
                     /old_locus_tag="MNU44_12325"
     CDS             8089..8232
                     /locus_tag="MNU44_RS12335"
                     /old_locus_tag="MNU44_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254105791.1"
                     /translation="MSNKYNIHPALYIGGFAVFFSFIFGFPIGTLMGLAIGIGISKKSE
                     NL"
                     /name="hypothetical protein"
     ARG             2955..4333
                     /name="mdeA"
                     /gene="mdeA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic; aminoglycoside
                     antibiotic; penam; tetracycline antibiotic; disinfecting
                     agents and antiseptics"
                     /identity="40.29"
                     /coverage="101.32"
     Typing          7125..7817
                     /name="rep_cluster_1442"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.455"
                     /coverage="80.0"
                     /evalue="0.0"
     Typing          5171..6447
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.418"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttagactct tgaaaactga ataaagatac aaaagttcac gtgatgaaaa ctcatgaaag
       61 cgcgtgaaat caatgatgta taaaatatga ataaacgata atcataagga aaaggcttaa
      121 acgttggttt cacagcgttt aagccttttt gtgtagttaa cataattaac tattataggt
      181 agtacttttg tatattctat tcatcttata attagctatc gatgtaaaaa ataatataaa
      241 agggccattc ttattaacag accattgtat gcttgtttag taaaaatgac attctccata
      301 ttatctgtat ctggagagag ctcagcttcc cttggtccgg gacttaaaat aggaatttta
      361 tttttatcat taaacttttc tttagatact attaagttat tttctaatga tggtttttca
      421 ttaacctttt ttgaataaga agagttaaaa tcaggtaaat caaacggaat aatatctaat
      481 atatcagctt gttctaatac tttctctaca ctatatacta tagaataatc agtatttttt
      541 aataaactta agatatcatt gtttaattct tctccgtatg gtaatagaaa tataatcttt
      601 ttaatactaa atttttctaa tcctattaca atagaacgaa tcactctaca attgatgtct
      661 cctcctactc ctatgatttt atcctctaag ccttttaatt ccttagacat cgtatataag
      721 tctaataaag cttgtgtagg atgctcatta gtaccatctc cagcattaat cataggaata
      781 tgggtcatct ttgaagcttt ttcaacagcg taattttgaa aatggcgcat aaccatgatg
      841 tctgagtact gttcaatata tttaatagta tctgacatac tctcatcaaa ataatcatca
      901 gtacgtgcta ctttaggtcc acccatgtct ataacttgac cacctaatct atacatagca
      961 cttgtaaagc tcattctagt tctagtactt ggttgataaa ataacgttgc aagaatttta
     1021 ccttctaaat ccttaattac ttttttatct tttaaagctt tttcataata atctgctttt
     1081 tcaaataggc tattaacatc attgagattc aaattttcca tcgacaataa attattaatc
     1141 aattatattt tcctctcttt ctacaatttt tgtttcccaa gaggctgaat caaacataga
     1201 ataacttgga gtcgaaggta attctttata ttctgggata tctttcaaat cagcaatttc
     1261 aaagcttaat ctttctacta aaagaccttg agctaaaggg ggacatccag caaaaattgg
     1321 acattcttta ttataaatta gccaatcatc taatctttct ttggatggaa actgagggta
     1381 ttctgggaaa agagagtaaa ctatttttct tgtattctgt tcgtccagtt ctgaacctgg
     1441 ctttatacca aaatagtctt cggctgacat tgagtcttta gtccatttaa aaaagaaagc
     1501 tctatgacca aatactggta cagttaacat aaatttacat ctttcactct ttttaaaagc
     1561 tttatgtagt gcataacgag cttctaattc aaacaattca attttatcta atacataatc
     1621 acaaccctct acaaaatcat ccgcagtact attgttaatt ccttcaggat aaacatcaac
     1681 atttacatct tttgaaatat taaatataga ttctccaact acttccgctt tatttcttcc
     1741 taaattatct aaagaggcgc caaactgtct attaatatta gagagttcaa aatgatctgg
     1801 atctgctact tttatatgac ggacacccaa tcgtactagt ctttcagcta cagcaccccc
     1861 tatacctcca catccggcaa tacctattac aacttcacta attttctttt gacgcttttt
     1921 cgcttcttca tcagtgtctc ctaaccaacc taaatttcgt ttaattcttt cccaatatcg
     1981 cttttcgtta aaatcagcag tatatttaat aactttagaa ttcattataa acctccaaaa
     2041 aataatgttt tataataaat acataaatcg atacggaaaa attaaaatat aagtggttta
     2101 atacacttac aataactaca taatcataaa actcttttaa ttgtcaaaat atttcgtttt
     2161 ctaattctgt aatgttcata tttaagtatg tcttaaaagc tttttttcct tttaaagtca
     2221 aattaagaca tctattgaat ttactttttt taacccaaga taattcaatc aacttttcag
     2281 ttatttgcaa tgcaatagga cctgctatat gatttttcct ttctgtccaa tctatatgtt
     2341 tgggaatagg ttttgaatac atagcttttt ctgttgcaat acctattttt tttaaccaaa
     2401 actttccttt ttgagtcaac ttataacaat ctttactttc tctaagataa tcattctcta
     2461 gcaaagcttc agtgaattta acacctattt tcccagcgat atgaccataa caagtacgca
     2521 tgtatgataa tttttctttt ttcgttgttt cttttaacga tttgctactc ataggtggag
     2581 atataataga taaagcatta attgcatcaa caatttcact attatcaatt ttatagtagc
     2641 aatgtcgtcc cctcttattt actgaaatta aattggcctt tactaatttt gaaagatgtt
     2701 cactagctgt ttgaggagtt acattagcta atttagctaa ttctccagct gggatagata
     2761 ccccacttga taattcgatt aacatagtta aacgtgtctt atgtccaata agtaaaatat
     2821 tatcagcaaa tttttcatac atataatgcc tcctttctat tagtacaatt taatcatacc
     2881 catataatat ttcggtgtta aacgaaatat tatatgggta tgattaaatt agttgaatga
     2941 ggaggtatga tagatgaatt cgtctaagta tttcgatcat ttagttgtct ttacagcagg
     3001 aataggtatg ttcttatcaa ctctagatag tggtattatc aatgtggcgt tacccacttt
     3061 atctaaatct tttaatgttg atgcttcttt tatttcttgg agtattacgt tatatacttt
     3121 gttacttaca gggaccatta ttatatttgg aagactgagt gataagtaca atcgtctaaa
     3181 tatctatagt attggactaa cagtgttttt gatatcttct attttatgtg gattttcaaa
     3241 taacgtggta gaattaatta cttttcgtgg tttacaaggc ataggtgcag ctatgttaca
     3301 aggcactgct acagctatta ttactacaac cattcctgag catagacagg gaccagcatt
     3361 aggaactttg agcattttat taggaatagg tcccgtgtta ggtcctagta ttggaggcct
     3421 tttaatatct ataggaaatt ggagatggat attttggatt aatattccat tcatatttat
     3481 aggcttaata ggctgtttac ttttaaaaaa atatattaaa gagccaaaaa gtgtatctat
     3541 tcatttagat atacgtggaa attcgctact atttgtatct atattttgct tattagtgag
     3601 tttaacatct tggtctcacc attcattttt taatatatca gtatatagca atttcttaat
     3661 atttattgta actttttgct tatttattgt gtgggaacta aaaactaatc atcctatcat
     3721 caatttacgt ttattcaaaa acatatcatt ttcatcttct atatttgcaa tatttgtttt
     3781 tggaggtaca acatctttag gctttataat tcctccatat attttagaaa aaatcaatca
     3841 tctttcatct tggcaaatag gactagtcaa tttgacatct cctttaggat tagtacttat
     3901 atcaaaaatc acagggaaat taatatctcg tgttggtaac actattttaa tgactgttgg
     3961 actagttatt atgattgctt catatactag cttagggtta ttacagtaca tattaaatcc
     4021 aataacaata agtctattgt tattgatata tggtataggt ggaggatttt tcttaccatc
     4081 taatacttca gcaatcatgg gaactgtatc taaagatatg caaggtactg tgggagcaac
     4141 tcaacggatg gttcaaaata taggtattgc agtttataca gcaattactt cactatttat
     4201 aagtaatcaa tcacagacaa atcaactgat aactggtgcc agccacgcat ggttattcgc
     4261 atcagcaaca cttttcttat cactattacc ttttttatta aaattattac accataagtc
     4321 agaagctact taatataata aaagcaagtg atttataaaa ttataaatca cttgctttta
     4381 tactgttctt ttaatcgttt aatcgcattt tttagtggac gatcatcacg atatgtattg
     4441 aagtttaaaa atgctgtact atcaaaatgt tgtaataagt gatgaaagac gtcattttct
     4501 tgtctcagtt ggtcgatttc atcttccaac tgatgaatat gatgctggag tgcctgattg
     4561 ttcgatttga gtgcctcgtt atctttttta aacaattcac tgacttcact ggattcttta
     4621 atttgttcga tgactgtcgg tttaatacga ttaccaatgg ctgtatgacg tatatattct
     4681 gtcacattca tctctcgaat atccgcaagt gcacaaatct tttcgtattg cgattgactt
     4741 acacgaatgg ttaagatttt ttcttttgaa ttttgttttt ttggcattga accacctcat
     4801 gattaatatg tgataagcat acatatgtgt gctaatcttt atattaatat aacatacata
     4861 tgtatgctaa taaatatgta tgattcgagg tggtatgagc taacatttgg catacagatt
     4921 ttcaaacata tttgtttgat aatgcgtatg tcatttgtta gctatagaac ggaattcaaa
     4981 aatatgtttt tgaatgtagt gatggcctcg tcagagcgaa gataactctt acatttgaat
     5041 gtaagagtat atcgagctat actctcattg cggaatcagc ctcatatgtt tgaggcgtag
     5101 cggtgagagt atccatcatt ttacataagc gaagcattgt cgaaggtaga caatcacagc
     5161 gcttgtaaaa tgatatttgc gaaggttctc gaaagtagag aaccacagac aaatcaaaaa
     5221 gcttcgggga gggcgctcaa tttgaaatta gcttggaata cgcaaaaaaa taaaatgagc
     5281 gatgttaaag gcaaagagat ggaacaagaa cgtaaaggga gaatcaaaaa cgatgagatt
     5341 gatttgaaac ggacaaactt aaactttgat ttagttgaag atgaacgtca tttgtatcat
     5401 cgtgtgaaag atagagtcga ttattataaa gaacaggggt cacgtgtaca gaaaaatagt
     5461 gtggtgatgt attccaatat cattacgtta tcgaaagaag aggccgaccg tatgggagaa
     5521 acacgtacaa aacactactt taagacatgt aaagattact tcagtgaacg ttttggcgaa
     5581 gccaatatcg tttcagcgaa agtgcatatg gatgaatcag cgcctcatat gcaccttcac
     5641 tttatacctg ttaatcatca aggacggtta tcagcacgta cagctatgaa tcgacaagct
     5701 attaatcaca tccatgatga attaacaaca catctgtgcc agcaaggttt tgatgttgaa
     5761 cgtgggagta ccgatgataa taaaacgtat attcaagata ttcatgaata taaaaagaaa
     5821 gcgaaagttt taagtgaatt agaccaacaa attgaaacga aaaaacaatt gttgagggat
     5881 atcaacgatt tacacgatga aaaagaacat ttaactcggg atattcaaaa cttaaaacgg
     5941 aaaaatcatt ttttacaaga ggattctcaa caggctcaag aagatattga cctatataag
     6001 caactgagag caaattctag ggacgatttt gaaaaagatg aggaaaagta ccaaaaagaa
     6061 atcaaagcgt atgaggcgtc tctgagtggc cttagaatga attaccgaga tttggacaag
     6121 cgcaatcaac atttaacaac acaaaataat caactcgaac accatttaga catgttaaat
     6181 gcacgtcatc aagcgatgaa cgacatggta agaaacgata cacgtatttt gtttgcgggg
     6241 cttagatgtg cattgaaaga taggaacatt gaattaagcg cagaaattaa atatcaacgt
     6301 atgccagaag tcggtattga tcccgatacg gtggatcagg gggctttaac acatgcgtat
     6361 caaggtgatg tatcagggat agaccatcat ataagaaagg catcatcact tattaaaaaa
     6421 gagacagaga atgacttaga attagaataa atcaataaaa ataagcactc aaacaagtat
     6481 gatactcgcc tgagtgctta tctcatgaaa agaggagata aaaatttaat aagtatgatc
     6541 aacctattaa gaacaatcat taccttactt acagcgctta ttaaatagtt ctgtaaggag
     6601 gaacaatacc tttgcatttc tatattatta taagaattcc aaggtagcaa agatattgta
     6661 ttggaagtga tagatgtgag tatgatttct aaattaattg atgaagatat tttgagtttc
     6721 taaacttaaa aaaatacctc cattctttta aaagtttatt tttgtgttgt tttctttata
     6781 aatatatttg aatacatttg aagtatttca ctccgacctt tttaatatat ttttataaat
     6841 ctttttaaaa cctttttaag tactgtttaa gacctagagg gacaaggctt ttgaaatgtt
     6901 aaaagggacg ttttgtaaca gtaaaaggga cgttttgtaa cagtaaaagg gacgttttgt
     6961 aacagtaaaa gggacgtttt gtaacagtaa aagggacgtt tttaaaatta tgtcacttat
     7021 attctttatc ctctatataa taggtgttat aattcatttg atcattatta tagagggacg
     7081 tttttaaaat tatgtcactt atatatttag agaggagttt tttatgtctg gtgaaacagt
     7141 agtatataga aatgagatga acttagtacc attaagacgt tttacaagta ctgaagtaga
     7201 tttattcttt acattatgca ataaacttaa agaacaggat acaagaaaag taattattcc
     7261 ttttgaagaa ttaaaatatt taagtaacta ctatacgcgt tctcaagaac gctttattaa
     7321 tgatttagag cacgtatacg ataaaatgtt gaatctaaca tatgtggaac gtaatggtag
     7381 gtcttttgaa aaatttgttt tatttacttc atacaaagta gatttagacg aagaatgttt
     7441 atctattagt gttaatcctg atttaaaaca tattttaaat tctataacag ctgattttac
     7501 gaaatttgaa ttaaaagaaa tgacccatct taaatctact tacgctaaaa atatgtttag
     7561 attgcttaaa caatataaac atactggtta tattaaaata aatatatctg attttaaaaa
     7621 tcgtctagat atacctaaag cttatcagat gaatgatata accaaacgtg tattaaagcc
     7681 aatatctaaa gagcttacac ctatttttaa taatcttaat attaataaag ttaaagctaa
     7741 aaaaggtcga aaaattgaat acttagaatt tatctttgac gctgagaaac gtattcacaa
     7801 caaacgacaa ccacaaataa ctaatatagg taagtcgcgc caatatacta atcgtgaaaa
     7861 gacaccagaa tggttaaatg aaggaagatt aggacaatct caagaaatac ataatgaaga
     7921 tgcaaaatta aaacaagatc gagaggcatt tcaacgtcaa ttagaagaaa aatgggagga
     7981 ttaataattt aaatgagcta catctatatt ttgcttttat ttcctttaat aatgggattt
     8041 atcgtatcaa taaaaaagaa aaaaatgact aaggagacat aagaaggtat gagtaataaa
     8101 tataatatac atccagcact atatatagga ggatttgcgg tattttttag ttttatattt
     8161 ggatttccta taggaacatt aatgggacta gctataggaa taggaattag taaaaaaagt
     8221 gaaaatttgt aaaagagttt acagatgaag aagttacacg atatactata attgacaaat
     8281 agcagatata tcattgtatg tgaaaacccc cagcgtgccg gctgggggtt tctttttact
     8341 tatgttcttg ttgtaataga taaagaacat acccaacgat aattggaaaa atgagcttat
     8401 ctaatacaaa tagcaagatt atcattgtat gtgacacctc ccttctgcac aggaagtgct
     8461 tttttagtat aacaaaattc ctctatactg cacgagttca catgatatgt atgcacgcat
     8521 acagtatgca aca
//
