LOCUS       NZ_CP093167            11811 bp    DNA     circular CON 20-JUL-2023
DEFINITION  Staphylococcus epidermidis strain TMDU-41 plasmid p2, complete
            sequence.
ACCESSION   NZ_CP093167
VERSION     NZ_CP093167.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26514226
            Assembly: GCF_024204705.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Saito,R., Ayibieke,A. and Yamada,K.
  TITLE     bloodstream S. epidermidis isolates from two Hospitals in Tokyo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11811)
  AUTHORS   Saito,R., Ayibieke,A. and Yamada,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2022) Molecular Microbiology, Tokyo Medical and
            Dental University, 1-5-45 yushima, Bunkyo-ku, Tokyo 113-8510, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method :: Unicycler hybrid assembley v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 283.0x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/20/2023 03:49:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,351
            CDSs (total)                      :: 2,267
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 34 of 52
            Pseudo Genes (incomplete)         :: 30 of 52
            Pseudo Genes (internal stop)      :: 18 of 52
            Pseudo Genes (multiple problems)  :: 23 of 52
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093167.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="TMDU-41"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p2"
                     /geo_loc_name="Japan"
                     /lat_lon="35.701735 N 139.7642509 E"
                     /collection_date="2015-01-21"
                     /collected_by="TMDU hospital staff"
     gene            complement(join(11611..11811,1..411))
                     /locus_tag="MNU56_RS11615"
                     /old_locus_tag="MNU56_11600"
     CDS             complement(join(11611..11811,1..411))
                     /locus_tag="MNU56_RS11615"
                     /old_locus_tag="MNU56_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_254104170.1"
                     /translation="MKTTKILGTATLAGALLFTGVGATHSNEAHADTQEITLPTPSEWQ
                     TQHDKLVQAKAEHPNQGGEGGGPGAITSYDKSYEEYIQKNLDYERKYGTVDFKIPQEGK
                     AYLNGQNSDNSNNQQSSQNNDQQAQNQATDNTVNTQNNDQQQTQTTDNNTTTQTNTQEQ
                     AKVLPETGESSNTTILTIVASVFLAAGSLLVFRRTSKSNK"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            complement(744..3932)
                     /locus_tag="MNU56_RS11620"
                     /old_locus_tag="MNU56_11605"
     CDS             complement(744..3932)
                     /locus_tag="MNU56_RS11620"
                     /old_locus_tag="MNU56_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016930563.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="WP_254104172.1"
                     /translation="MQTVKQKNSKLYINFEEFNKSVQNMYVKNEFDSLSFEKNTENQSF
                     IVDLEKVIDVFDKYATSKLYIVVEEKTPILTTQKNLKVNNKRTEVYHLSEVTYKGMKIY
                     PYITKNGFLHLSMSEYEPNKVYFSRRHIDDMKINNSMAFLKGQFSILNSNLEEAQLLIK
                     TRFTEKETLVNISLDNLVQKSNISTYNFSVDIYSELIEFMKYPFENEDVIDIFLNIKIQ
                     ESEEAIKVKLGNPRILVERLLKGEIITNFNDEIVSITPYLTMKGRNLSFRINRYSLDSY
                     LSYIESVKKSSIVNFIKKSDEKIWVIGEKSYKAQDNGYHFFKYLRINHPEIPAYYIIEE
                     NSKEAKNVLPLGNVIFYKSPEHFDIMLKADYICTTHHPELIYPTNSSIYTRKIKATKVF
                     LQHGVLGTKNLRNINGNHLKDFNVDLFITSSQREKKIVVRDLNFYENQVKVTGLARFDE
                     LFDSNTNVKDQIIIIPTWRDWLINIEQLKKSEYLRRINSLLYSSKLKELANQGTDIVFC
                     LHPNMQPFVDLFDIPPYVSSVKQGEIDVQRLIKESKLMITDYSSVAFDFSFLKKPVIYY
                     QFDKSDFLGKEPSHIDIEAELPGIIVNSQDDLEKELNRLKNKGYVIDQDIEKRMNKFIE
                     NRDKQNSYRIYKEIIDYKNKSKIKNKVKYDILTKHLFKRFRKSKKYFKVMDILNTLLTK
                     ITPTQQDLIVFESNVGKSVSDSPKVIYEELQKQSKKYKIVWVTNKQYPFNESNVISVKR
                     LSPEYYYYLSRAKYWINNQNFPHYINKSKDTIYIQTWHGTPLKKMLNDVDSFKGRDKGY
                     KYRVNHAIKYWDYLISPSPYASACFKTAFNFKKDILEIGYPRNDIFYKENADDLENKKN
                     KVKQQLGIKDNRKIILYAPTFRDNEITKAKKHIIDLKLDLQKMKQNFEKEYILIIRPHI
                     IISNSMKLEDNLENFVVNAANYNDISDLYLISDICITDYSSVMFDFANTKKPLLFFTYD
                     LDFYKKELRGFYFDFEKEAPGPLIKTNDELVNSIKNIQHIKAEYQAKYDEFYKRFCTFE
                     NGTAAKEIVNRFFK"
                     /name="CDP-glycerol glycerophosphotransferase family
                     protein"
     gene            4043..5470
                     /locus_tag="MNU56_RS11625"
                     /old_locus_tag="MNU56_11610"
     CDS             4043..5470
                     /locus_tag="MNU56_RS11625"
                     /old_locus_tag="MNU56_11610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="WP_254104174.1"
                     /translation="MKKPLFSIIITTFNNSNTIKQTLDSVINQTLDNSKYEIIVVDDNS
                     TDNTLDILKSYNVSNLKIHQLNSNSGGPSKPRNVGIDYSRGKYVHFLDGDDWLDLNVLQ
                     NITSKYYNLKSDIIIAKVIKYKDGNQTIHAKFMTTKENLNKKANDIPYLYYYLGPSGKF
                     IKRKLILKKHLKFPYDLHFGEDKLFFMNVFASAKTVTTIPTICNYLNRSSDNTSIVKKS
                     DFITKRKSDLFVFQSALNIPNKSCRDKFLLRIVQYDLLNNCNSNVFLRLNDSQQLEVFN
                     IIKKMYENDYIKKYIIPLIDSKYHNAINAIYNNDLDKFVQFFEWYKHGTKILNKLDKKT
                     YVQESTCNYNFEIIVPFANTKNLQFDNQKVYLQVNYHNLNENQINGILLDSRSNFRNSI
                     LLNNFSIKQGLLSIQLDYMLLNKLVNGIYNVLIIYDDYKTLNIKYGYTKNIETKSNSLT
                     FYPTINGNISIKCQTFN"
                     /name="glycosyltransferase family A protein"
     gene            5487..5675
                     /locus_tag="MNU56_RS11630"
                     /old_locus_tag="MNU56_11615"
     CDS             5487..5675
                     /locus_tag="MNU56_RS11630"
                     /old_locus_tag="MNU56_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254104176.1"
                     /translation="MKKLLLFFNLITLCIFFYKQKQTQKKYNISLVDIESEQDILDENG
                     LYKDENGNILPIIERFE"
                     /name="hypothetical protein"
     gene            complement(5801..6967)
                     /gene="tarF"
                     /locus_tag="MNU56_RS11635"
                     /old_locus_tag="MNU56_11620"
     CDS             complement(5801..6967)
                     /gene="tarF"
                     /locus_tag="MNU56_RS11635"
                     /old_locus_tag="MNU56_11620"
                     /EC_number="2.7.8.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458391.1"
                     /GO_process="GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid glycerol-phosphate transferase
                     TarF"
                     /protein_id="WP_254104178.1"
                     /translation="MKAIINNLIKICIKAIFAIISQTKNNKLVYFESFHGKQYSDNPRA
                     LYEYLNQNTDNNLIWGVKKGYESAFEKHGVPYVIRFSLKWFLTMPKAKVWIINTRLPQW
                     FYKSSKTIYLQTWHGTPLKKLGLDIEDVYMSGANTTKYKKDIIDESKRWDFLISPNEYS
                     TDNFEKAFRINRSKIIETGYPRNDKLINEQNNVSYIQRIKDDLNIPAHKKVIMYAPTWR
                     DDKFIREGTYELNLNLNIDKLRQQLSDDYVILLRMHYLVVTRFKETDSFIIDVSDYEDI
                     AELYLISDILITDYSSVMFDFGVLKRPQIFYAYDLEDYDSRLRGFYMDYKNNLPGPIVY
                     NENDLIHLLKKEEDLKKKYNKELEQFYKLYCSFENGDSSKRVIDRIMK"
                     /name="tarF"
     gene            7325..7708
                     /gene="mobC"
                     /locus_tag="MNU56_RS11640"
                     /old_locus_tag="MNU56_11625"
     CDS             7325..7708
                     /gene="mobC"
                     /locus_tag="MNU56_RS11640"
                     /old_locus_tag="MNU56_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_254104164.1"
                     /translation="MSEQNTFVASDETVGRNRKPNRKEPKQISFRVSESEYLKLQQSAE
                     TLNMSVPAFVKKKAQGARLVAPKLDQATRQSVAKDFSMLGANANQIAKYCNQHQHEAPN
                     YEALERNISELRERLDEIWKTLK"
                     /name="mobC"
     gene            7711..7887
                     /locus_tag="MNU56_RS11645"
                     /old_locus_tag="MNU56_11630"
     CDS             7711..7887
                     /locus_tag="MNU56_RS11645"
                     /old_locus_tag="MNU56_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_239665741.1"
                     /translation="MIVSAVLLVVCLAIASFLIFHEQQIFPSLIFLGLAFIDGTKFYLS
                     YQKLKERDHHGDN"
                     /name="hypothetical protein"
     gene            7874..8884
                     /locus_tag="MNU56_RS11650"
                     /old_locus_tag="MNU56_11635"
     CDS             7874..8884
                     /locus_tag="MNU56_RS11650"
                     /old_locus_tag="MNU56_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_254104166.1"
                     /translation="MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKAARE
                     LYGKTDGNQGHVIIQSFKPGEVTPKQCNQLGLALAEKLAPNHQVAVYTHNDTDHVHNHI
                     VINSIDLETGKKFNNNKQALRDLRNFNDEVCRENGLSVPEKDTARLRYTQTEKAITDPN
                     TKSTAQYSWKDEIREAIDQSQATNMDEFKDHLNQHGIEIERVTPKSITYRHLAEDKKVR
                     GRKLGEDYNKGGIEHGFERQIQRQKQTERASDTDVQPKRRTSSRDEETERDTGVTQADW
                     DKFAQDINELERRRQAAESARLVDEKARRDREERAREKQASRTIINDDRDYGLEL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9006..9392
                     /locus_tag="MNU56_RS11655"
                     /old_locus_tag="MNU56_11640"
     CDS             9006..9392
                     /locus_tag="MNU56_RS11655"
                     /old_locus_tag="MNU56_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002477845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_254104180.1"
                     /translation="MKTDTHKAIEQLQTNQAELRQANNDYKKMIDEHIKHNDTAMKQYD
                     QALNRLTKGITAIFFIVALVMIAFLALSPLGDWLGVQHFYEWLNYVLKTGHSAWRYLMI
                     VFYLVPYALFGLLIYAILSAYKRI"
                     /name="mobilization protein"
     gene            10403..11260
                     /locus_tag="MNU56_RS11660"
                     /old_locus_tag="MNU56_11645"
     CDS             10403..11260
                     /locus_tag="MNU56_RS11660"
                     /old_locus_tag="MNU56_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458740.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_254104168.1"
                     /translation="MSGETVVYQNEMNLVPLRKFTSTEIDIFFSLCNKLKEQDTKELTI
                     PFEELRHLSNYYHRSLKRFVKDLEHVYDKILTLTYTERSGLSFKKFVLFTGYEVDVDKQ
                     ELIVSINPKLKHVLNEITADFTKFELKEMTHLKSTYSKNMFRLLKQYKHTGYFKIQIDD
                     FRERLDIPNSYRMTHINQKVLAPIIKELSFIFNNLNINKIKAKKGRKIEWLEFTFDAEK
                     RIHNKRQPQMANIDKSRQYISREKTPKWLEERTYEKSTHNEYDPQLEKEREAFLKKLEV
                     DWEE"
                     /name="RepB family plasmid replication initiator protein"
     Typing          complement(7117..7354)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.361"
                     /coverage="100.0"
                     /evalue="4.7e-95"
     Typing          10404..11259
                     /name="rep_cluster_2222"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.406"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          7872..8881
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.285"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tacagtgtta tcagttgctt gattttgagc ttgttgatcg ttattttgag atgattgttg
       61 attattagaa ttatcactat tttgaccatt taaatatgct ttaccttctt gaggtatttt
      121 aaaatctaca gttccatatt ttctttcata atctaagttt ttttgaatat attcttcata
      181 acttttgtcg taactagtaa tagcaccagg tccgccacct tctccacctt ggttagggtg
      241 ttctgcttta gcttgtacca atttatcatg ttgtgtttgc cattcagaag gtgttggtaa
      301 tgtgatttct tgtgtatctg catgtgcttc gttagaatga gttgctccta cacctgtgaa
      361 taataaagcg cctgctaatg ttgctgtacc taaaatttta gttgttttca taaaaacact
      421 ccttaatatg tatttaatcg tacagtagaa ttttaacaca gtaaacttat taaaaaaata
      481 atagatagtg taatttaaaa tttattaaac aaatatatgt ataagcattt gatatccttg
      541 aaagacatga tctaaggtat atttatacat aatcaattaa aatgaaaata catgaaaacg
      601 cgtgagatca atgatgtata aaagatgaat aatcgataat ccaaacacaa agggacaaac
      661 gctgaaatga cggcgtttgt ccctttgtgt tgcgttgaca taattacgta ttataggtag
      721 taatgttgta taaatattgt atatcactta aaaaatctat ttactatttc ttttgcagct
      781 gttccatttt caaacgtaca aaaacgttta taaaactcat catattttgc ttgatactct
      841 gcttttatat gctgaatatt ttttattgaa ttaaccaatt catcattagt tttaatcaaa
      901 ggacctggag cctctttttc aaagtcaaaa taaaatcctc taagctcttt tttataaaaa
      961 tctaaatcat acgtgaaaaa taataatggt tttttcgtat ttgcaaaatc aaacataact
     1021 gaagaatagt ctgtaataca tatatcagat attaaatata aatcactgat atcattataa
     1081 ttagctgcat taacgacaaa attttctaaa ttatcctcta acttcataga gttacttata
     1141 ataatatgtg gtcttattat taaaatatat tctttttcaa aattttgctt cattttttgt
     1201 aaatctaatt ttaaatctat aatatgtttt ttagcttttg taatttcatt gtctctaaaa
     1261 gtaggagcat acaatataat ctttctatta tctttaatac caagttgctg tttaacttta
     1321 ttttttttgt tttctaagtc atctgcattt tctttataga aaatatcatt tctaggataa
     1381 cctatttcaa gtatatcttt tttaaaattg aaagctgttt taaaacaagc actagcatat
     1441 gggctaggag agataaggta atcccaatat ttaatggcgt gattaactct atatttatat
     1501 cctttatctc gacctttaaa tgaatcaaca tcatttaaca tctttttaag tggtgtacca
     1561 tgccatgttt gtatatatat agtatcttta gatttattaa tatagtgagg gaaattttgg
     1621 ttattaatcc aatatttcgc tctagataaa taataataat attctggtga taatctctta
     1681 acagatatta cgttagattc attaaatgga tattgcttat tggtaaccca cacaatctta
     1741 tatttttttg attgcttttg taattcttca tatatgactt tcggactgtc gctcacagat
     1801 ttccctacat tactttcaaa tactattaaa tcttgttgcg taggtgttat tttagttaat
     1861 agcgtattta atatatccat taccttaaaa tattttttac tttttctaaa acgcttgaat
     1921 aaatgcttgg tcaaaatatc atatttaact ttatttttta ttttactttt gtttttgtaa
     1981 tcaataattt ccttgtatat tctgtaagaa ttttgcttat ctctattttc aataaattta
     2041 ttcatccgtt tttctatatc ttgatcaatt acataacctt tatttttcaa acgattaagc
     2101 tctttttcta ggtcgtcttg tgaatttact ataattcctg gaagttcagc ctctatatca
     2161 atatgagagg gttctttacc taagaaatca gatttatcaa attgataata aataacaggt
     2221 tttttcaaaa aactaaaatc aaacgcaaca cttgaataat ctgtaatcat taatttactt
     2281 tctttaatta atctttgaac atctatttct ccttgtttaa cacttgaaac gtaaggtggg
     2341 atgtcaaaaa gatctacaaa tggttgcatg ttaggatgta aacaaaaaac aatatcagtt
     2401 ccttgattcg caagttcctt taatttagag ctatacaaca aactatttat tcttcttaaa
     2461 tactctgatt tttttaactg ttcaatattt attaaccaat ctctccatgt aggaataata
     2521 ataatttgat ctttgacatt ggtattagaa tcaaatagct catcaaatct agccaatcct
     2581 gttactttaa cttgattttc ataaaaattt aaatctctaa caactatctt tttttctctt
     2641 tgagaactgg taataaataa atctacatta aaatctttta aatgattacc attgatattt
     2701 cttaaatttt tagttcctaa aactccatgt tgtaaaaaaa ctttagtcgc ttttattttt
     2761 cgagtataaa ttgaactatt agttggatat atcaattcag gatgatgtgt agtacaaata
     2821 taatcagcct ttaacataat atcaaaatgt tcaggtgact tataaaatat aacatttccc
     2881 aaaggaagta cattttttgc ttctttggaa ttctcttcaa ttatataata tgcaggtatt
     2941 tctggatgat taattcttaa gtatttaaaa aaatgatacc cattatcttg agctttatag
     3001 gacttttctc ctataaccca aattttttca tcagattttt ttataaagtt aactatacta
     3061 ctttttttaa cactttctat atatgataaa taactgtcta aactatatct attaattcta
     3121 aatgacaaat ttctaccttt cattgttaaa taaggtgtaa tagatacgat ttcatcatta
     3181 aaatttgtaa taatttcccc ttttaataat ctttccacta atattcttgg atttcctagt
     3241 ttaactttta tagcttcttc tgactcttgt atttttatat ttagaaaaat gtcaataaca
     3301 tcttcatttt caaaaggata cttcataaat tcaattaatt cgctataaat atccacacta
     3361 aagttatatg ttgatatatt gcttttttgt actaaattat caagactaat atttactaaa
     3421 gtttcttttt cagtgaatct cgttttaatt aataactgag cttcttctaa attagagttt
     3481 aaaattgaaa attgaccttt taaaaaagcc atactgttat ttattttcat atcatctata
     3541 tgtcttctag aaaaataaac tttattaggt tcatattcac tcatagataa atgtaagaaa
     3601 ccatttttag ttatataagg atatattttc attcctttat atgtaacttc agataaatga
     3661 taaacttcag ttcgtttgtt attaactttt aaattctttt gagttgttaa aataggggtc
     3721 ttttcttcta ctacaatata taattttgat gtagcatatt tatcgaatac atcaattact
     3781 ttttctaaat caactataaa actttgattt tcagtatttt tttcaaaaga caatgagtca
     3841 aattcatttt ttacatacat gttttgtaca cttttattaa attcttcaaa atttatatac
     3901 aacttcgaat ttttttgttt taccgtttgc aaatactttc cctccattgg tataaaaatt
     3961 attctattag ttttaacaat atatacgtta caatagaaat acattcttaa ctataaaatt
     4021 taaaatattg gagaatattc agatgaaaaa acccttattt tcaatcataa taacaacttt
     4081 taataattca aacactatta aacaaactct ggattcagtt attaatcaaa ctttagataa
     4141 cagtaaatat gaaattatag tagttgatga taattctact gataatactt tagatatatt
     4201 gaaaagttat aatgtaagta atttaaaaat tcatcagcta aattcaaata gtggtggacc
     4261 atctaaacca agaaacgtgg gtattgatta tagtagggga aaatacgttc attttctaga
     4321 tggtgacgat tggttagatt taaatgtatt acaaaatatt acttcaaaat actataattt
     4381 aaaaagtgat attattatag ctaaagtaat aaagtataaa gacggcaatc aaactataca
     4441 tgctaaattc atgacaacaa aagaaaattt aaacaaaaaa gcgaatgata taccttattt
     4501 atattattac ttaggaccaa gtgggaaatt tattaaacgt aagttaattt taaaaaaaca
     4561 tttaaagttt ccttatgact tacatttcgg agaagataaa ctttttttta tgaacgtttt
     4621 tgcttctgcc aaaactgtaa ctacaattcc aacgatttgt aactatttaa atagatcttc
     4681 tgataacact tctattgtaa aaaaatccga ctttataact aaaagaaaaa gtgatctatt
     4741 cgtatttcaa agtgctttaa atataccaaa taaaagttgt agagataaat ttttattacg
     4801 aatagtccaa tatgatttat taaataattg taattcaaat gtattcttaa gattaaatga
     4861 ctctcaacaa ttagaagtat ttaacattat aaaaaagatg tatgaaaatg actatattaa
     4921 aaaatatatt attcctttaa tagattcaaa atatcataat gcaataaatg ctatttataa
     4981 taatgattta gacaaatttg tacagttttt tgagtggtat aaacatggaa caaaaattct
     5041 aaataaatta gataaaaaaa cttacgtaca agaaagcacg tgtaattata actttgaaat
     5101 aatagtacct tttgctaata ctaaaaattt acaatttgat aatcaaaaag tttatcttca
     5161 agttaattac cataatttaa atgaaaatca aatcaatggt attttattag atagtcgttc
     5221 caattttaga aattctatac tgttaaataa tttttctata aaacaggggt tattatcaat
     5281 tcagttagac tatatgttac tgaataaatt agttaacggt atttacaatg ttcttattat
     5341 ttatgatgac tataaaacat taaatattaa atatggttat actaaaaata tcgaaactaa
     5401 aagtaatagt ttaacttttt atcctactat taatggtaat atttctataa aatgccaaac
     5461 ttttaattaa aaggagaata attatcatga agaaattatt attgtttttt aacctaataa
     5521 cattatgtat atttttctat aaacaaaaac aaacccaaaa aaaatataat ataagtttgg
     5581 ttgatataga atctgaacag gatatcttag atgaaaatgg tttatataaa gatgaaaatg
     5641 gtaatatact ccctatcata gaacgatttg agtagtaata attttatgat ttatagacat
     5701 taactagcta actatctagt aacaaaagga cctatgtttt aaaatgtaag tccttttgtt
     5761 ttacttgaaa attattttga atattttaag atataatatt tcacttcatt attctatcga
     5821 ttactctttt tgaagaatct ccattttcaa aagaacaata taatttataa aattgttcta
     5881 attctttatt atatttcttc ttaagatctt cctctttttt caataaatga attaaatcgt
     5941 tttcattata aacaatagga ccaggtaaat tatttttata gtccatataa aatcctcgta
     6001 atcgactatc atagtcttct aaatcataag cataaaaaat ctgtggtctt tttaatactc
     6061 caaagtcaaa catgactgaa gaataatcag ttatcaatat atcgctaatt aaatacaatt
     6121 cagcaatatc ttcataatct gaaacatcga taataaaact atcagtttct ttaaaccttg
     6181 taacaactaa atagtgcatt cttaataaaa taacatagtc atcactcaat tgctgtctaa
     6241 gcttatcaat atttaaattt aaatttaatt catatgttcc ttctcttata aacttgtcat
     6301 ctcgccatgt aggagcatac attataacct ttttatgagc tggtatattt aaatcatctt
     6361 taatacgttg aatataagac acattatttt gttcattaat taacttatca tttctcggat
     6421 aacctgtttc aataatttta gaacgattta ttcgaaaagc tttttcaaag ttatcagttg
     6481 agtattcatt aggagaaatt aaaaaatccc aacgtttact ctcatcaatg atatctttct
     6541 tatatttagt tgtatttgcc cccgacatat atacatcttc aatatctaga cctaattttt
     6601 ttaatggtgt accatgccat gtttgtaaat atattgtttt tgaagactta tagaaccatt
     6661 gtggtaatct agtattaata atccaaacct ttgcctttgg cattgttaaa aaccatttca
     6721 atgaaaatcg aataacatat ggcaccccat gtttctcaaa tgctgactca taaccttttt
     6781 ttacacccca aataagatta ttatctgtat tttgatttaa atattcatat aatgcacgtg
     6841 gattatcact atattgtttt ccatgaaaac tttcaaaata cactaattta ttgtttttag
     6901 tttgagaaat aattgcaaaa attgctttaa tacatatttt tatcaaatta tttataattg
     6961 ctttcaacgt cttaaacctc cttaataata aaaataacat tccattaaat aatgtctata
     7021 tcaaacattt tactaaaaag attttaaacc ttgaagaaga aataatatat gctaaggagt
     7081 aacaataata attaatattc acatttttta tttatagtgg ctatcaatta gccactattt
     7141 tttcgctaga aaaaatccat aaggggcttg gggatgttcc ccaacaagct ggcgcgtctg
     7201 ccacgttagt ggctagcaaa gccaatgctt gccaaaccac ttaacgactt gattaatttt
     7261 gctttaccct gtaaagtaaa atgtaagaaa ggcgttatct ttcgaagaaa gcgtggtggg
     7321 aataatgagc gaacaaaaca catttgtggc tagcgatgaa actgttgggc gaaaccgtaa
     7381 acccaaccgt aaggagccaa aacaaatcag ttttcgtgtg agcgaatccg aatatttaaa
     7441 gttacaacaa tcagctgaaa ctttaaatat gagtgtgcct gctttcgtta agaaaaaggc
     7501 acaaggcgcc cgattggtcg cacccaaatt ggatcaagca acgcgacaat cggtagcaaa
     7561 agattttagt atgttgggcg caaatgccaa tcagattgcg aaatattgca accaacatca
     7621 acatgaagca ccaaactacg aagcattaga acgtaatatc agcgaattac gtgaaaggtt
     7681 agatgagata tggaaaacac taaagtaaca atgattgtaa gtgcggtatt attagttgtt
     7741 tgtttagcaa ttgctagttt tttaattttc catgaacaac aaatttttcc aagtttgatt
     7801 tttttaggct tagcttttat tgatggcact aaattttatc tgtcttatca aaaattgaaa
     7861 gaacgtgatc atcatggcga caactaaatt aagtgccacc aaatcaacat cacgagcgat
     7921 taattacgct gaaaaacgtg cagtcgaaaa aagtggctta aattgtgatg tcgactatgc
     7981 caaatcgagt tttaaagctg ctcgtgagtt atatggtaaa accgatggca atcaagggca
     8041 tgtcattatc caatcgttta aacctggcga agtcacgccc aaacaatgta atcaattagg
     8101 attagcactc gctgaaaaac tcgcacccaa tcatcaagtc gctgtgtaca cacataatga
     8161 tacagatcac gtacacaatc atatcgtaat caattccatt gacctcgaga caggtaagaa
     8221 atttaacaac aataaacaag ccttacgtga tttaagaaat ttcaatgacg aagtctgtcg
     8281 ggaaaacggt ttgagtgtgc cagaaaaaga cacagcacgc ttacgttata cgcagacaga
     8341 aaaagcgatt actgacccta atacaaaatc aacagcccaa tattcatgga aagatgagat
     8401 tagagaggcg atagaccaat cacaagccac gaatatggac gaattcaaag atcatttaaa
     8461 tcaacatggg attgaaattg aacgtgtcac gcccaaaagt attacgtatc gacatttagc
     8521 cgaagataaa aaagtacgtg gccgtaaact tggtgaagac tacaacaaag gaggcataga
     8581 acatggcttt gaaagacaaa tacaacgaca aaaacaaaca gaacgagcat cagacaccga
     8641 cgtccaaccc aaacgacgaa cttcaagtcg tgatgaagaa actgaacgag atacaggtgt
     8701 cactcaagca gattgggaca agttcgccca agacataaat gagcttgagc gtcgccgaca
     8761 agctgcagag tcagcaagac ttgttgatga aaaggctcga agagatcgag aagagagagc
     8821 acgagagaaa caagcgtcac gaacaattat taacgacgat cgagactacg gcctcgaact
     8881 ttaatcaagc gactgaaatc acacaaaaac gtttcatcag tgtagcgaaa cactatatcg
     8941 aacgcattga taacgataat ttaaagcaag attttcaaac ggcgatacaa gaagaattgg
     9001 aagatgtgaa aacagacacg cataaggcaa ttgagcagtt acaaaccaac caagccgaac
     9061 ttcgacaagc caataatgat tataaaaaaa tgatagatga acatatcaag cacaacgaca
     9121 cagccatgaa acaatatgat caagcgttga atcgtttaac caaaggcatt acagcaatat
     9181 tttttattgt tgccttagtc atgatcgctt ttttagcgct cagtccgtta ggtgactggt
     9241 taggtgtgca acatttttac gaatggttga actatgtcct gaaaacagga cattcagcat
     9301 ggcgttactt gatgattgta ttctatcttg ttccttatgc tttatttgga ttgcttatat
     9361 atgccatttt aagcgcgtat aagcgtattt aatatgaata ttggaccaaa accacgtttt
     9421 tgataatgga aaagctatat caagcttaaa ataacttaga aattaaaggg agatcctatt
     9481 ttataaaaag gaggaactat gagaagagag ttatttttaa gtgtcgtctt tttaatgttt
     9541 atatttataa ttttgatatt ttcggattat atatttttaa atgatctcta ttatttgaaa
     9601 aatgctttag tgtctatagc tataactata tttatcatgt gcatgattga aaaaaagtga
     9661 aataaaaaaa gacgggcgtg cccgtctcta aggaattatc caaacaatag agttacttcc
     9721 atgttttgtt tgcaaaatat agtattagac tttacaccta tataatagca aaaaatcagc
     9781 tatatataaa ctatcaaaac tcaaactgtt attttacaat gcaattatgc cgagaaaatt
     9841 tattgagcat tgagaagaac ccttaattaa actgtttcga cgaatgtcgg tatagcgtaa
     9901 gctatgaatc cgacgagtcg acagtttttg ggtttattaa gggttcagag gctcaatgtc
     9961 aataaagcaa ttggaataat gcattaaagt tatatcactt attacttttt taaataataa
    10021 aaaccaacca caaatatggt tggtttcctt tttgtctttt gaatttgtga ggggcaaatg
    10081 ccccgaactt tttaatatct ttttataaat ctttttaaaa cctttttagg agtcatttga
    10141 gccttagagt gacaaccgat ttgaaatgac aaaggcaact ttttgtaact acaaaggcaa
    10201 ctttttgtaa ctacaaaggc aactttttgt aactacaaag gcaacttttt gtaactacaa
    10261 aggcaacttt ttttagaaaa gttgcctttg tagttacatt aatgttatta taattttaaa
    10321 ttaaagtatt tacatactat taaggcacct attttaaaaa aagtgtccta attttgttta
    10381 ttgcttagga ggctttataa acatgtctgg agaaactgtt gtctatcaaa atgaaatgaa
    10441 tttagttcct ttacgtaagt ttaccagcac agaaatcgat atatttttta gtctttgtaa
    10501 taagttaaaa gaacaagata caaaagaatt aactatccct tttgaggaat tacgtcactt
    10561 aagtaattac tatcatcgtt cattaaaacg ttttgttaaa gatttagaac atgtatacga
    10621 taagatatta actttaactt atactgaacg aagtggccta tctttcaaga aatttgtatt
    10681 gtttacagga tatgaagttg atgttgataa acaagaatta attgttagta ttaaccctaa
    10741 attaaaacac gttttaaatg aaataacagc tgattttact aagtttgaat tgaaagaaat
    10801 gacacacctt aaatcaacat actctaaaaa tatgtttagg ttattaaaac aatataaaca
    10861 cacaggctat ttcaaaattc aaattgatga ttttagagaa cgattagata ttcctaatag
    10921 ttaccgtatg acacatatta atcaaaaagt attagcccct atcattaaag aattaagttt
    10981 tatttttaac aaccttaata tcaataaaat aaaggcaaaa aaaggtcgca aaattgaatg
    11041 gctagagttt acgtttgacg ctgagaaacg tattcacaat aaacgacaac cacaaatggc
    11101 gaatatagat aagtcacgcc aatatataag tcgcgagaaa accccaaaat ggttagaaga
    11161 acgcacatat gaaaaatcaa cacacaatga gtatgatcca caactagaga aagaacgaga
    11221 agctttttta aaaaaacttg aagtggattg ggaagaatag aaaagtttac aaaaaataac
    11281 atgaaagata tactaaaagt aacaagtgaa gtatggttcg ccatatgcaa aaaccccact
    11341 gccgaccaaa gcaaagtggg gtttttttta tttatctttt taggttatct gtaatgtagt
    11401 tcgcaactac accagcgata acagataaga taaatgttcc aacaagtgaa agtatcgtta
    11461 acgccatatg cacacctcct ttcatgcaca ggaagtgctt ttttagtata acaaaattac
    11521 tcttataatt tttaagatgt taaatttatg ggatttgaag atataaaaag agcacaaagc
    11581 ttcgctttgt gctcttttta taattaatta ttatttgttc gatttagaag tacgtctaaa
    11641 tactaataat gaaccagcag ctaaaaatac tgatgcaact attgttaaga ttgtagtgtt
    11701 tgatgattca ccagtttctg gtaaaacttt agcttgttct tgagtattag tttgagttgt
    11761 agtgttatta tcagtagttt gagtttgttg ttggtcgtta ttttgagtat t
//
