LOCUS       NZ_CP093071             2617 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Escherichia coli strain BR1220 plasmid p2.6, complete sequence.
ACCESSION   NZ_CP093071
VERSION     NZ_CP093071.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26512579
            Assembly: GCF_022569415.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Argueta,F., Tatarenkov,A. and Mota-Bravo,L.
  TITLE     Multiple Copies of a qnrB19 Gene Are Carried by Tandem Repeats of an
            IS26 Composite Transposon in an Escherichia coli Plasmid
  JOURNAL   Microbiol Resour Announc 11 (12), e0066122 (2022)
   PUBMED   36394337
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Mota-Bravo,L.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2022) School of Biological Sciences, University of
            California, Irvine, 1106 Biological Sciences III, Irvine, CA
            92697-2527, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-OCT-2020
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.3x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022569415.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:44:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,212
            CDSs (total)                      :: 5,091
            Genes (coding)                    :: 4,805
            CDSs (with protein)               :: 4,805
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 100 of 286
            Pseudo Genes (incomplete)         :: 201 of 286
            Pseudo Genes (internal stop)      :: 58 of 286
            Pseudo Genes (multiple problems)  :: 65 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP093071.1.
            Bacteria and source DNA available from PI Luis Mota-Bravo.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BR1220"
                     /isolation_source="Feces of Canada Goose (Branta
                     canadensis)"
                     /host="Branta canadensis"
                     /db_xref="taxon:562"
                     /plasmid="p2.6"
                     /geo_loc_name="USA: Lincoln Square, New York, NY"
                     /lat_lon="40.773536 N 73.993613 W"
                     /collection_date="2018-08-07"
                     /collected_by="Luis Mota-Bravo"
     gene            <534..689
                     /locus_tag="MM431_RS25850"
                     /old_locus_tag="MM431_25850"
                     /pseudo=""
     CDS             <534..689
                     /locus_tag="MM431_RS25850"
                     /old_locus_tag="MM431_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            783..1427
                     /locus_tag="MM431_RS25855"
                     /old_locus_tag="MM431_25855"
     CDS             783..1427
                     /locus_tag="MM431_RS25855"
                     /old_locus_tag="MM431_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(1847..2083)
                     /locus_tag="MM431_RS25860"
                     /old_locus_tag="MM431_25860"
     CDS             complement(1847..2083)
                     /locus_tag="MM431_RS25860"
                     /old_locus_tag="MM431_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     ARG             784..1424
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2249..2422)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          10..481
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.2"
                     /coverage="95.0"
                     /evalue="6.38e-127"
     Typing          2395..2617
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="93.0"
                     /evalue="2.430000000000001e-86"
ORIGIN
        1 atgcggctta aagataccag gcgtttcccc ctggtggctc cctcgtgctc ccccgcattc
       61 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttatct cattccacgc
      121 ctgacactca gttccgcgaa ggcagttcgc tcctggatgg actgtatgca cgaacccccc
      181 gttcagtccg actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga
      241 cacgacttat cgccactggc agcagccact ggtaactgat ttagaggagt tagtcttgaa
      301 gttatgcgcc tgttaaggct aaactgaaag aacagatttg gttactgcgc tcctcgtaag
      361 ccagttacca cggttcaaag agttgctgct ctgagaacct tcgaaaaacc gccttgcagg
      421 gcggtttttt cgtttacaga gcaagagatt acgacgccac agaaaggatc tcaagaagat
      481 ccttcaccgt aatggaaatc tatccaatcc gagcacatag gatacacatc gttataacgc
      541 tggatctgcg tgaattccag cagcagcaag agaaagactt tctacaaacc agcttacagc
      601 aggctaaatt taaccagaaa aaagccgctg aattactggg tctgacttat catcagctac
      661 gtgccttgct gaagaaacac cagatctaat acgcatttgc aggtgtcata ctggcgggtt
      721 tgacgcataa cgtcataagg tttaccatgg cgtcattact gtataaaaac acaggcatag
      781 atatgactct ggcattagtt ggcgaaaaaa ttgacagaaa tcgcttcacc ggtgagaaag
      841 ttgaaaatag tacatttttt aactgcgatt tttcaggtgc cgacctgagc ggcactgaat
      901 ttatcggctg ccagttctat gatcgcgaaa gtcagaaagg gtgcaatttt agtcgcgcaa
      961 tgctgaaaga tgccattttc aaaagctgtg atttatcaat ggcagatttc cgcaacgtca
     1021 gtgccttggg cattgaaatt cgccactgcc gcgcacaagg cgcagatttc cgcggtgcaa
     1081 gctttatgaa tatgatcacc acgcgcacct ggttttgcag cgcatatatc actaatacta
     1141 atctaagcta cgccaatttt tcgaaagtcg tgttggaaaa gtgtgagctg tgggaaaacc
     1201 gctggatggg gactcaggta ctgggtgcga cgttcagtgg ttcagatctc tccggcggcg
     1261 agttttcgac tttcgactgg cgagcagcaa acttcacaca ttgcgatctg accaattcgg
     1321 agttaggtga cttagatatt cggggtgttg atttacaagg cgttaagtta gacagctacc
     1381 aggcatcgtt gctcatggag cggcttggca tcgctgtgat tggttagttc agcgtttaca
     1441 ccgggagatg cgtttgacga tttactttta aggggttcct gtatctaatt ttttgctgga
     1501 cattcatacc cgcgatcatc gcgtttattg agtttattat ttatctatgc gattccgatg
     1561 aaaaattcgc acgaaaatat ggctaactca tagcaaaaaa aaagccccct aagggctttt
     1621 ttttgtacct gacggtcaga ccgattaaac ataacatccc ttgcccgcac ccccgcaggc
     1681 gccctcagcg caatcctgct gcatcagggc cgtttccagc acctttcctc accagtatcc
     1741 cggaaaaacg cccctgatga tttctgcgcg actgcatggc tatgcacgca ggtgaatggc
     1801 aggtgatttc gcagcgtcag caccactaaa ccgccaaaag ccaaaatcag cggcgcggcg
     1861 cgcgcggacg tgaccaggca aaatcggact gctgccgctc atcctcacgt aatgcacgct
     1921 ggcggtcggc gtaacgctgc aactcaattt gcgcacgcgc tctgttcagg acgatgtgcc
     1981 ggttaggctg atttagcttt ttacctgacg ctctttcagc tgcggcaaca gcatcatcaa
     2041 gaaatttttt ctctgcaggc gtcaagtttg tcagtctggc cataacgttc tccagtcaga
     2101 ataaatcagc acagtctgcc agacacagta gaaacgatga agcgccagcg acggtgaaag
     2161 gcagtcagaa acggtgaacg ggagcggatt ttaatccggt gccgtgaggc gttggacaag
     2221 ccgcaggcgc gtcattagct tttagccggt ttcggggcgc agccctgaac cagtcacgta
     2281 gcgctagcgg agtgtatact ggctgactat gttggcacgg atgtggaaat cagtgaagtg
     2341 cttcatgtag caggagaaaa aaggcggcac cagcgcgtca gcaggatatg tagtacagga
     2401 tatattccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2461 tgccggctta caggcggggc ggaaatttcc tgggagatac caggaaggag cttaacaggg
     2521 aagagataag gccgcggcga agccgttttt ccataggctc cgcccccctg acaaacatca
     2581 tgaaatctga cgctcaaatc agtggtggcg aaacccg
//
