LOCUS       NZ_CP092910             3011 bp    DNA     circular CON 07-APR-2024
DEFINITION  Enterococcus faecalis strain LJX 0909 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP092910
VERSION     NZ_CP092910.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26281488
            Assembly: GCF_022557275.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Liu,R. and Fan,W.
  TITLE     Metformin significantly reduce the alkali resistance of standard and
            wild E. faecalis in normal and high glucose environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Liu,R. and Fan,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2022) School and Hospital of Stomatology, Wuhan
            University, Luonan Street, Wuhan 430075, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unlcycler v. V0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 255.1x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022557275.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 08:03:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,843
            CDSs (total)                      :: 2,765
            Genes (coding)                    :: 2,717
            CDSs (with protein)               :: 2,717
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 24 of 48
            Pseudo Genes (incomplete)         :: 17 of 48
            Pseudo Genes (internal stop)      :: 16 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092910.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="LJX 0909"
                     /isolation_source="Wuhan"
                     /host="The State Key Laboratory Breeding Base of Basic
                     Science of Stomatology"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Hubei"
                     /collection_date="2022"
     gene            join(2923..3011,1..73)
                     /locus_tag="MK744_RS14115"
                     /old_locus_tag="MK744_14115"
     CDS             join(2923..3011,1..73)
                     /locus_tag="MK744_RS14115"
                     /old_locus_tag="MK744_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002328004.1"
                     /translation="MVISESKKRVMISLTKEQDRKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
                     /name="hypothetical protein"
     gene            141..899
                     /locus_tag="MK744_RS14120"
                     /old_locus_tag="MK744_14120"
     CDS             141..899
                     /locus_tag="MK744_RS14120"
                     /old_locus_tag="MK744_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_073438348.1"
                     /translation="MAVKNTKARHFGFLLYPDSIPNDWKEKLESLGISMAVSPLHDMDE
                     KKDEDTWNSNDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSIAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKNILHINDFDLDRYITLDESQKRELRDQLVEII
                     NQCGIVNLKELKAFLDWRGQEFGISNQRDVTDVIGSNGNLFRMAFDGNYQNGFRPQYAR
                     RVDPETGETISGVDELISKVKEGEENKGEL"
                     /name="replication protein"
     gene            complement(1378..1806)
                     /locus_tag="MK744_RS14125"
                     /old_locus_tag="MK744_14125"
     CDS             complement(1378..1806)
                     /locus_tag="MK744_RS14125"
                     /old_locus_tag="MK744_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005917942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="WP_241408357.1"
                     /translation="MSNEIQQRNSDTYNMTPFNFFEDFSRNLFNDFKPNLIKTDIHETD
                     NEYFVEAELPGIPKENIQVTYENGVLTISGQHQIDKATEDKKGRLLRSERSLTSVRRQY
                     LLENVKEDEIKASYSEGILKLTLPKDSNKEIKKAIPIE"
                     /name="Hsp20/alpha crystallin family protein"
     gene            complement(1906..2160)
                     /locus_tag="MK744_RS14130"
                     /old_locus_tag="MK744_14130"
     CDS             complement(1906..2160)
                     /locus_tag="MK744_RS14130"
                     /old_locus_tag="MK744_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epsilon-antitoxin"
                     /protein_id="WP_002328003.1"
                     /translation="MGLDYRKTFEIEIINEFQSAIHSKMLNFVLNNEFDKSDSKNPQTN
                     LLNQLSNMNQINLFKLSLEELEAYHEYLRAIKKYADSIT"
                     /name="epsilon-antitoxin"
ORIGIN
        1 tttttcaaaa tctgcggttg cagcgttagc tatagaagaa tatgcaagaa aggaatcaga
       61 acaaaaaaaa taagcgaaat ctcgcgtttt tagaaggata cgagttttcg ctacatgtgt
      121 tttgataagg taattatatc atggctgtta aaaatactaa agctagacat tttgggtttt
      181 tattatatcc tgattcaatt ccaaatgatt ggaaagaaaa attagagagt ttaggcattt
      241 caatggctgt tagtcctcta cacgatatgg atgagaaaaa agatgaagat acgtggaata
      301 gtaatgatgt tatacggaat ggaaagcatt ataaaaaacc acattatcat gttatatata
      361 ttgctcgaaa tccagtaaca atagagagtg ttaggaataa gattaagaga aaactgggaa
      421 atagttcgat tgctcatgtt gagatacttg attatatcaa aggttcatat gaatatctta
      481 cgcatgaatc gaaagatgct attgctaaga ataaacatat ttacgataaa aaaaatattt
      541 tgcatattaa tgattttgat cttgaccgct atataacact tgatgagagt caaaaaagag
      601 agctgagaga tcaattggta gagattataa atcaatgtgg aatagtcaat ttgaaggaat
      661 taaaggcttt tcttgactgg agaggacaag agtttggaat ctctaatcaa agagatgtga
      721 ctgatgtaat tggttcgaat gggaatcttt ttcgcatggc ttttgatggt aattaccaga
      781 atggttttcg cccacaatat gcaagaagag tagaccctga aactggtgaa actatcagtg
      841 gtgttgacga actaataagt aaagttaaag aaggagaaga aaataaaggt gagttataaa
      901 gatttttttg ataaataatt ttagacaaga gcgaagcgaa ttttaacaaa cacgcttgac
      961 ggaaaaagag tacagtttca cgtgagtgat ctgtactctt ttttagcacg atagcaacca
     1021 aaatattttg gtgggtgtaa gtgcgccctt agcacaacgt agaaaaaatt cggtagattt
     1081 ttttactgcg tataagtgcg ccctttggta tataggtata taaaaatgat agaaaaggag
     1141 tggtactatg tttcaagcca tagcttttga aactgaaaga ctgaaattct gtgctgcaac
     1201 accaaatgat attaatataa ttatgggaat ggagacccat gttgataatg gttattaaga
     1261 tcaaattatt tattcgattt tagatagtga atggagaaag aaaaaataat ttatttttac
     1321 gaaaaaaagg cgtctataaa attttatagg cgcctttttt tcgtaatttt ttaattacta
     1381 ctcaattgga atagcttttt ttatttcttt gttactatct tttggcaagg ttagtttaag
     1441 aattccctct gaataagaag cctttatttc gtcttcttta acattttcta ataaatattg
     1501 acgtcggaca cttgttaaac tacgttcgct acgaagcaac cttccttttt tatcttcggt
     1561 tgctttatca atttggtgtt ggccactaat tgttaatact ccgttttcgt aagtaacttg
     1621 aatgttttct ttaggaatac caggaagttc agcttctaca aaatattcat tatcagtttc
     1681 atgaatatct gttttgatta aatttggctt aaaatcattg aataaattac gactaaagtc
     1741 ttcaaaaaaa ttaaaaggtg tcatgttata tgtgtctgag ttacgttgtt gaatctcgtt
     1801 tgacataata atcaatccct tctatatttt cttttcaatt attattttag tcaaaaaagg
     1861 tcagttttca agtattaagc tatttgaaaa ttgtgaaatt atttttcatg tgatgctatc
     1921 agcatatttt tttatcgctc gtaaatactc atgataggct tctagttctt caagagataa
     1981 tttaaataaa ttaatttgat tcatgtttga taattgattt agtaagtttg tttgtgggtt
     2041 tttagaatca cttttatcga attcattatt taaaacaaag ttcaacattt ttgagtgaat
     2101 cgctgattga aattcattga ttatctcaat ttcaaatgtt tttctgtaat ctaatcccat
     2161 gttgtttgct cctttctggt ttcgctttgc tcaactaaac atttactttt aattaaacag
     2221 acttcgctag tttaatcaaa agtaaacgtg cgaataaaaa gcaaatcatt tttaaagctc
     2281 ataaaatacg ttctaaggcg ttttgattct tctaggcata tttttatact gttttgtttt
     2341 taaaaagcct gtatcagcct aaaaatgcgt tctaaggcgt tttagcgttt atttcgttta
     2401 gttatcggca taatcgttaa aacagcggtt ctcgtaacgt aaaagccctt gagcgtagcg
     2461 tggctttgca gtgaagaggt tcgctgttag attatgaaag ccgataactg aatgaaataa
     2521 taagcgatag cccactattt cggtaggaag aggctcaagg gagtttgagg gaatgaaatt
     2581 ccctcatggt cttatggttt aaaaaattgc ttgcaatttt gccgagcggt agcgctggaa
     2641 aatttttgga aaaaatttgg aatttgtaaa aaatgggggg cgaggaagcg aattttgctt
     2701 ccgtactacg accccccata gtgtgccgag tgccaatttt tgtgccaaaa tctcttgaag
     2761 ctaacagggg caaggggttt ggtcactttt gaatcgccaa cgaatcgcca acgttttcgc
     2821 caacgttttt tttaaatcta tatattagtt tctttattgt tgcttatata gctacaaagt
     2881 gatacactat ttttataaaa ttatttgata ggggtttttt taatggtgat ttcagaatcg
     2941 aaaaaaagag ttatgatttc tctgactaaa gagcaagata ggaaattaac agatatggca
     3001 aaacaaaaag g
//
