LOCUS       NZ_CP092909             5107 bp    DNA     circular CON 07-APR-2024
DEFINITION  Enterococcus faecalis strain LJX 0909 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP092909
VERSION     NZ_CP092909.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26281488
            Assembly: GCF_022557275.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Liu,R. and Fan,W.
  TITLE     Metformin significantly reduce the alkali resistance of standard and
            wild E. faecalis in normal and high glucose environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Liu,R. and Fan,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2022) School and Hospital of Stomatology, Wuhan
            University, Luonan Street, Wuhan 430075, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unlcycler v. V0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 255.1x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022557275.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 08:03:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,843
            CDSs (total)                      :: 2,765
            Genes (coding)                    :: 2,717
            CDSs (with protein)               :: 2,717
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 24 of 48
            Pseudo Genes (incomplete)         :: 17 of 48
            Pseudo Genes (internal stop)      :: 16 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092909.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="LJX 0909"
                     /isolation_source="Wuhan"
                     /host="The State Key Laboratory Breeding Base of Basic
                     Science of Stomatology"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed2"
                     /geo_loc_name="China:Hubei"
                     /collection_date="2022"
     gene            complement(join(3567..5107,1..139))
                     /gene="mobV"
                     /locus_tag="MK744_RS14090"
                     /old_locus_tag="MK744_14090"
     CDS             complement(join(3567..5107,1..139))
                     /gene="mobV"
                     /locus_tag="MK744_RS14090"
                     /old_locus_tag="MK744_14090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_010715975.1"
                     /translation="MSKIVARMEKMKVGNLSGIQRHNQRETDNHSNPDIDIEKSHLNYD
                     LVNHGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQSLLEIQEELPN
                     YLKERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLEAKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDLSAIKSITKDFETLRTQNKHLKIDNNTLTQENKELKQVNHVQELQITDLTHE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDKTTQKVSVLETDNKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGQAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            complement(915..1211)
                     /locus_tag="MK744_RS14095"
                     /old_locus_tag="MK744_14095"
     CDS             complement(915..1211)
                     /locus_tag="MK744_RS14095"
                     /old_locus_tag="MK744_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364284.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLTRLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLLDTVVYKDTLEEDIACGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            complement(1339..2067)
                     /locus_tag="MK744_RS14100"
                     /old_locus_tag="MK744_14100"
     CDS             complement(1339..2067)
                     /locus_tag="MK744_RS14100"
                     /old_locus_tag="MK744_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002364286.1"
                     /translation="MKKDTELTVKYRNELNMITLKKFNAKEMDLFFALCARMKDKGLNN
                     IVFSFEELKELSDYRTTAIKAFTDDLDSLYSKMLQLTYRDEYDDDGSFRRFVLFTSFDV
                     NVKKQTVEVSINPKLEGILNGLTTEFSKFELSAFTSLRSTYAKTLFRLLMQYRSTGYYV
                     VNIEEFRELLNIPDYYQMGNIDQKVLKPAMKELNNYFEDLEITKIKAKKGNKIAKLEFT
                     FTGLKTTKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            2672..2758
                     /locus_tag="MK744_RS14105"
                     /old_locus_tag="MK744_14105"
     CDS             2672..2758
                     /locus_tag="MK744_RS14105"
                     /old_locus_tag="MK744_14105"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(3278..3478)
                     /locus_tag="MK744_RS14110"
                     /old_locus_tag="MK744_14110"
     CDS             complement(3278..3478)
                     /locus_tag="MK744_RS14110"
                     /old_locus_tag="MK744_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     Typing          complement(3571..5105)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.289"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 ctaagtcata attcaagtga gatttctcaa tatcaatatc aggattggaa tgattatcgg
       61 tttctcgttg attatgccgt tggataccgc ttaagtttcc aactttcatt ttctccatac
      121 gtgcgacaat ttttgacatg agcgctcccc cccttttttt tcttgcggcg ttctttgcct
      181 ttggatttcg gcagtacttc caagtaaagt ataacgcact atactttact tcgtaaaggt
      241 gcgctctgcc gagggctttc gtctagcact cgaaaggtca gcctttaggc ttcccaatct
      301 cgtacttagc cgattgccta cggtcaaact ttccatttct gctaaggcag aaactccaaa
      361 tttccctccg tcaaaaaacc gtttaaataa cgactgcctg aaattagtga acgtgctagc
      421 tttctgctag ccggtgctag taatgtgcta atttggtgct aaaaacctgc tagtcactgc
      481 taggaatctg ctagtttggg tgctagatac ctgctagtga gtgctaaaaa tgtgctagtt
      541 tacctgaaag tacctgctag taaagtgcta gcgaagaacg aaagtgtacc ccacgggaaa
      601 gccttgcact gcaaggcggg gcaccttgtg tgccccccag ttccccttat gctcttccga
      661 aacacaggaa gaaattaagc aaatttcaac agtaaaagca aaaaataaca gcagtgggat
      721 tgggggaacc ccaaaaattt gaacgactcc taccagtatt tttggtaggg gtcattcaaa
      781 atatcgcaac atgctagcat attgctctgc tcgcaaaaag aaagcctacc ccaaaatgga
      841 agtaggctaa tcttgatgtt tttcgagtat tatcccaaat agtcaacatt ttttataaca
      901 agcaaggcta gtttttattc ttcttcgtct agtgattggc cgtcaatctt cacgattcca
      961 caggcgatat cttcttctag cgtatctttg tagacgaccg tatcaagaag tagaatgtat
     1021 gaccgactta gataattaaa cattcttagc gtttcgtctc gaactacagt atctaatcgt
     1081 gataagtcaa gcggctccaa atcaagcaat agcgcttcta gttcactttc aaacttatct
     1141 agtcgagtca gtacagcctg tgtgacttcg tactcctctt ttgtcatgtt gtatcctgtc
     1201 attaatccca tgtatatgac ctccaaaata atttatttta gaagtcctca cgcaaatatg
     1261 ctaaaataat ctagcagtcg tagtggattg cgtgaggagc ttgctaactt gcttaatgtg
     1321 agggggcaag ttcttttttt attctccgtt tacccagtca tgcatagtta cgcttggttt
     1381 ggtggttttc aaaccagtaa acgtaaattc tagtttggca atcttattgc cttttttggc
     1441 tttgattttt gttatttcta aatcttcaaa gtagttattg agttctttca ttgctggttt
     1501 taaaactttt tgatcaatat ttcccatttg ataataatcg ggaatattca ataattctct
     1561 gaactcttca atgttaacta cgtaatatcc tgttgaccga tactgcatta acaaacgaaa
     1621 caaagttttt gcataggttg agcgtaacga agtaaatgct gataattcaa atttactaaa
     1681 ttcagttgtt aaaccattaa gaataccttc taacttaggg tttatactta cttcaactgt
     1741 ttgttttttt acgttaacgt caaagctagt aaataaaacg aatcgtctaa agcttccgtc
     1801 atcatcatat tcgtcacgat atgttagttg taacatttta ctgtacaaac tatctaaatc
     1861 atctgtgaac gcttttatcg cagttgttct atagtcgctt aattctttca actcttcaaa
     1921 cgaaaaaaca atgttattta agcctttatc tttcatgcgt gcacataaag caaagaatag
     1981 gtccatttct ttagcgttga acttttttaa ggtaatcatg tttaattcgt ttcgatattt
     2041 aactgttaat tccgtgtctt tcttcatcct ttcgccccct cacactatga attttaaaac
     2101 aaacaaccaa ctttgtcaac ggttggttgt ttacagagaa aaagttgtta gtactcagag
     2161 aaaaagttgt tagtactcag agaaaaagtt gttagtactc agagaaaaag ttgttagtac
     2221 tctccgctct tccttagagc cacgtggccc acagaccgcc taaaaaggtt ttaaaaaata
     2281 tataaaaaat atataaaata aacgcgcgtg cgcgcgagga aatttcccgc ctaaaaattt
     2341 cctctttttt ctacaaacag gttcgcgata tttacgaaac tgtcactaaa aaatcgaaaa
     2401 acaaaaaaag gagtgagcga agttcttcgc tcacattatg cttgcagcgt tcacttcgtt
     2461 cactcgctaa aatcaaaatc atttattaag gaactctttg tatagtctct atttgcttgt
     2521 ctgaagctct ttaagacatg tttagttgtt ttagggtatc ttggttagga ttgagccttg
     2581 caggcggtca gggcaagccc taacaacccc cacaagaaat tcttcttgtg atatacttgt
     2641 tttagcaact gccccgaaag ggggtgtaaa catgattgaa agcttaattt cgttgattat
     2701 cgccccactt tttgtgggtt tagtactttt gctagttgat cgttggctag acgattagca
     2761 gataatctat gcagttgctt ttctagctcc aacgctgaaa tcgcaacagt gtaaaaaaag
     2821 ccctcctact gagaatagga gggctttttg gtgtaaacat gattttgctt aatttcgctt
     2881 cgcacctaca caataacatt ttttttctgt attttcaaac gtcagattag attacgcaaa
     2941 acgtcaatta gagaaatacc atatcgaaat agttagtatt aaacatagta atactatgag
     3001 gatagacaat attttacttt ttggactatc ttcaaagctt ttattttctg gtctgtaagt
     3061 gggattcact actttcgcgt tgtaatccat tgtacttccc aaatatactt ctgaaggaat
     3121 taaaaaaaat aataaaattc cgtaaaaaat aattatcaat atgggcaaat caatattaac
     3181 taaaaaagaa gttatatagc ttaaaactaa aggaatagta atataaaata tattttttat
     3241 tagatatttt ttcatgaaat aacctcaaat ttagcaacta ttttgtccat cttgtatgca
     3301 ttgggataca aaatccaccg gctttatgat atcctcctgc agtccacccg ccttttttcc
     3361 aataaaatcc tggctgacac tctatagaat cccactctct agcacttgct tctgtagttg
     3421 aacttaaagc aacagagata aaagacaccc ctaaaattag aactaacaac tttttcataa
     3481 tgcttacctc actttgtttt aatttttatt aatatatttt aatttaactg tttttatgtg
     3541 tcaataattt atcaattaaa ttatttttaa cgagataaac ctcgtccttt tgacatatct
     3601 cttgaatatt ttttactctg ttgccaagcc tttttatctg tcgactgttc aaacgtaaaa
     3661 gctttcttct ctagcacttg ttcttcagac agctgtaaag tatttgtgcg cgaacgtcca
     3721 ttagccatat actcgccgac aaactcataa caccctgttt ctctgttgta aaacggggct
     3781 tttagggggt agaaatgtgg gtttaaggac attgttaccc cctttgaccc cgtatctggt
     3841 ggtaaaaaca atgtcccttt ttcgtcaatg aggtaagttc tatccttgga atatcgctcc
     3901 agggattttt ttaatttagg cgcgttctga aggtacttaa ctccttgctg aatggtaaat
     3961 aaaatggttt ctttggcctg tcctagtctt tcatgaaggt aagtggttaa cccgtcaaac
     4021 tttaatttta tttcttgata tttttcgttt agttcagaaa agtcgtaggt taagtcagta
     4081 attttatctt ttaaccgctt attatctgtt tctaacacag aaactttttg tgttgtttta
     4141 tcaagttctt gtttgatttg ttcgctctgg tcatagttta gttgtttttc ttcaaacgct
     4201 gttaattgct ctttttttag ggtcaattca tgggttaagt ccgttatttg taattcttgt
     4261 acatgattta cttgtttcaa ttctttattt tcctgtgtca aggtattgtt atcgatttta
     4321 aggtgcttat tttgcgttct aagcgtttca aaatctttgg taatactttt tatcgcagaa
     4381 aggtcagaag cgcttaaaac gtattttaaa ccgtttaaac taaatttctt ttctttgaca
     4441 tttgctaaat ctggtaaaac ggttgtttgt agagagttaa tgatttcgtt tcgcttcgct
     4501 tccagttggt tcattgcttg gactttgccc tttacttctt cgttcttctc ttcaagaagg
     4561 cggtcaatac ggaagattgc ttgttcataa gaatggagtt ctctctcttt ttctgccaga
     4621 atttgttcct gattagctag ttcctgctta gaaacctgta gctccttttg aacgtctttg
     4681 tattctttga cagataaatg cttttgcgga ctcccacgga gtccacgctc aatagcgtag
     4741 ccccgttctt tcaaatagtt tggcagttct tcctgaatct ctaacaaact ttgtcgacta
     4801 aacacctgtt tactgctcaa tcgcccttcg tacataggca caattcctaa gtgcatatgg
     4861 ggcgtggttt cgtctaaatg taccgtggca taggcgacat tttgtcgacc gcagcgatca
     4921 gagaaatacg cgacaacatc ggtaaaaaaa gcttgtgtgt ctgtattctc ttgaaaaaaa
     4981 gcggtatcac tagtgattat ccattcgttc acaaggactg cgtcttttct aaccgctcgt
     5041 ttactgattc gttggctctc aatgatttgt ttgattttct cccgataatt gattgaacca
     5101 tgattga
//
