LOCUS       NZ_CP092791             5596 bp    DNA     circular CON 23-MAY-2024
DEFINITION  Klebsiella pneumoniae strain XH1508 plasmid pXH1508-5, complete
            sequence.
ACCESSION   NZ_CP092791
VERSION     NZ_CP092791.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26270981
            Assembly: GCF_022533505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     The genome sequence of Klebsiella pneumoniae XH1507 and XH1508
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2022) Sir Run Run Shaw Hospital, Zhejiang
            University, Qingchundong 3 RD, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.48
            Assembly Name                     :: XH1507
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022533505.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 06:11:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,985
            CDSs (total)                      :: 5,864
            Genes (coding)                    :: 5,679
            CDSs (with protein)               :: 5,679
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 67 of 185
            Pseudo Genes (incomplete)         :: 125 of 185
            Pseudo Genes (internal stop)      :: 25 of 185
            Pseudo Genes (multiple problems)  :: 32 of 185
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092791.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="XH1508"
                     /isolation_source="pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pXH1508-5"
                     /geo_loc_name="China:Hangzhou"
                     /lat_lon="30.3 N 120.2 E"
                     /collection_date="2019-05-10"
                     /collected_by="Xiaoting Hua"
     gene            170..328
                     /locus_tag="ML259_RS29975"
     CDS             170..328
                     /locus_tag="ML259_RS29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            686..1012
                     /locus_tag="ML259_RS29730"
                     /old_locus_tag="ML259_29730"
     CDS             686..1012
                     /locus_tag="ML259_RS29730"
                     /old_locus_tag="ML259_29730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1038..1583
                     /locus_tag="ML259_RS29735"
                     /old_locus_tag="ML259_29735"
     CDS             1038..1583
                     /locus_tag="ML259_RS29735"
                     /old_locus_tag="ML259_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            1649..1999
                     /locus_tag="ML259_RS29740"
                     /old_locus_tag="ML259_29740"
     CDS             1649..1999
                     /locus_tag="ML259_RS29740"
                     /old_locus_tag="ML259_29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2069..2335)
                     /locus_tag="ML259_RS29745"
                     /old_locus_tag="ML259_29745"
     CDS             complement(2069..2335)
                     /locus_tag="ML259_RS29745"
                     /old_locus_tag="ML259_29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2319..2543)
                     /locus_tag="ML259_RS29750"
                     /old_locus_tag="ML259_29750"
     CDS             complement(2319..2543)
                     /locus_tag="ML259_RS29750"
                     /old_locus_tag="ML259_29750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3210..3422)
                     /locus_tag="ML259_RS29755"
                     /old_locus_tag="ML259_29755"
     CDS             complement(3210..3422)
                     /locus_tag="ML259_RS29755"
                     /old_locus_tag="ML259_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3429..3938)
                     /locus_tag="ML259_RS29760"
                     /old_locus_tag="ML259_29760"
     CDS             complement(3429..3938)
                     /locus_tag="ML259_RS29760"
                     /old_locus_tag="ML259_29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3973..4677)
                     /locus_tag="ML259_RS29980"
                     /pseudo=""
     CDS             complement(<3973..4677)
                     /locus_tag="ML259_RS29980"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4616..4960)
                     /locus_tag="ML259_RS29770"
                     /old_locus_tag="ML259_29770"
     CDS             complement(4616..4960)
                     /locus_tag="ML259_RS29770"
                     /old_locus_tag="ML259_29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4950..5126)
                     /locus_tag="ML259_RS29775"
                     /old_locus_tag="ML259_29775"
     CDS             complement(4950..5126)
                     /locus_tag="ML259_RS29775"
                     /old_locus_tag="ML259_29775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          complement(5213..5289)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          complement(59..239)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.956"
                     /coverage="90.0"
                     /evalue="3.08e-71"
     Typing          complement(3095..4626)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccggtgatt tctctccggc ggctccctcg tgcgctctcc tgttccgacc ctgccgttta
       61 ccggatacct gttccgcctt tttccctttc gggaagcgtg gcgctttctc atagctcacg
      121 ctgctggtat ctcggctcgg tttaggtcgt tcgctccaag ctgggctgta tgcacgaacc
      181 ccccgttcag cccgactgct gcgccctttc cggtaactat catcttgagt ccaacccgga
      241 cggacacgca aaaacgccac tggcagcagc cattggtaac tggatatata cgagagagat
      301 attgagaggt cttgaagtgg gtgcctaact gcggctacac tgaaagaaca gttttggtat
      361 ctgcggtctc gtatacccag ttacccggtt aagcagttcc ccaactgact taaccttcga
      421 tcaaaccgcc tccccaggcg gttttttcgt ttacagtgca agagattacg cgcagaaaaa
      481 aaggatctca agaagatcct ttaaatgatc gtcagttctg ttatccccgg agctccatcc
      541 atcccacgga aaaacacccg ccactgatgc cctcagcgca ctgcacatca tgtacaaacc
      601 actccctccc tcctttttta ttgcgtttct taatgcgcaa tggtagcttt gtgcgcaaca
      661 aaaaacgcaa tagcaagggg gggtaatgag agaaagaaca aatatcatgc tagatatagg
      721 attaaagcaa tccttacaac ggctttcgaa taaaaccaaa atgagtctaa gtgatcttat
      781 taaccagaga ttatcaggtt cactttcagt tgaagaacgc attggcgaaa agcctgaatg
      841 gatgagaaac cttgatccct atctcgccaa tcacttttca aatattgagt taccgctccc
      901 ttcaccatta aaagaatacc aagaaaaaat ggatgagata gattcaatta ttaatgacat
      961 gatggttcta aaggaatatt tcagagctaa gttttacgac gaaataaaat aggcacgcta
     1021 tacggaggcg gagaattatg ttttttaaac ggtatttaac ggagaaggaa atatacaagg
     1081 cagcactccc aattgttagg cttcaaggga gagttaacac ttcattacgt tttttatcca
     1141 aatactcaga tgtaaacaaa aatggattaa ttgcattgca tcttaattta ttacatttta
     1201 tctcatacaa catacacggt gtagagtctc tgaaaatcac tcaagcaggc ataaacaaag
     1261 ctgtacaaca aagccctcac gcagatgaaa taaaaaatat tgcgcaggca ataggacgca
     1321 aaggacctga gtacatcgaa ggattaatta gaaacccatg ggggaagaaa aatgataatc
     1381 cactaatatt tataatgagc attgaagaaa gtataaaaga aacccttaac tcaggttata
     1441 tgttatatag agtagctccc ataactgagg gtgaagcagc tgctttcatg gatgttctta
     1501 aacaattatt attatttgag gataattttc gcctttttgg tagagacttg gtttataaaa
     1561 tagaaaaatt aaaaatcaga taacaaagta tggtgaacga aaaatgaaag agaggatagt
     1621 gaaatgtgta ataccaagat tctcaactat gtattggtta attgcgttcc tagcatttat
     1681 attccccggt cgatcttttg ctgacggtta caccgcttac taccgatgta atggtactca
     1741 ggaagcatct cttactgtta aagataacaa actaagtatg gttgtaggtc tttctaagtt
     1801 taataggcaa ctggatactt atagattcaa agaaaaggga atcgaagtag taatgcagag
     1861 agctgcttct gatgataact ctgaggccat aatgtctttt attatgcctg acgttaacaa
     1921 tatgacctta ttagttaaaa ataacggcta cattatcgtt aagacaacct gctggttaac
     1981 taaaaccgac aaacagtaga acacgtagtt agtcgagtat ttaccataat acctgttgtc
     2041 ggcatcaaaa aagcccgcat agcgggcttc agtcgtaaac ttcgcggcga tgtccaatcg
     2101 tggcggcaag gataacaagc tcgtcatctc ggatctcaca cagaacgcga taatctccca
     2161 cccgatagcg ccagaactcg cccagctcac ctttgagcgg cttccctgac tggcgaggat
     2221 cggcagcaac tgcaatgcgt aaactcataa aatccacaat ccgtcgtgcg ttctgtttgt
     2281 ccatcttgcg taacgatttg agcgcccgat cggaatagtt aatcgtccag accaagtgac
     2341 ttcctcacgt cttcagacga atgcactgct tcatcaccac ggcgaacgcg tgcagcagtt
     2401 tctgctgaaa gataataatc ctccaggtct tccagatgcg ccagtattgc ttcccgcgca
     2461 taaaacgtct ttgttctgcc ggtttgcttc gccagcgagt ccagacggga ctcaatctca
     2521 tcagataacc ggatagcaag catataaacc tcccgaacac catttgctat acatgtatat
     2581 caacagtacc acaatcaacc taaaagaaca aaaaataatc ttgttagccc ctcttgataa
     2641 tccgtaacgc ggtcaatata tctagcttga gggctttcct cttaactatt gaaaggataa
     2701 cgattatggg tcttaagccg ggtccgaaac gaattgccga ttcaacaggg gaacctgata
     2761 aacgccagcg tgacaataaa aaaacgccgg gaaatactga caaattgaaa cccagcaaat
     2821 cttcaaaaaa ataagtcagt agttaaaagc cctccggggc ttttttgtat ctgccgttca
     2881 cgctgattaa acataacacc cgttgcccgc accgccccgg acgggttcgc cgcgaacggg
     2941 gtggcagtca ggtaaaaccg gcaacaaacc acccgataaa ccgccccgtt cgcgacagat
     3001 aaattttacc tggctgcata gccatgcagc caggtgaatg acatccggtt tcagcgtaaa
     3061 tgcccgatga gaaccctgtt ttcctgcatt tcagcccagc gacggaccgt catagcttcg
     3121 ctgccgggaa cgcgcttccg tcagttcctt ttcgtgctgc cgttgctcgt gcgcccgttc
     3181 tgcggccacg gccatctcat gacgctgcac taccggctca aacgctgcag ttgcccgctc
     3241 aagcgttcca cctgctgact caagcgcgtc acgcgctcgc tcagcgccgc gttctcccgt
     3301 tgcataagcc ctgacatttt ccgccattcc ccgaaggcgc tctcccactc gtccagcctt
     3361 ttcgagtagt cctgctgtag ctgctctaat gcgctcagca actgtttttc cagctcggtc
     3421 atcggcactt actccttcac cagtctcagc gcgttctgct tcgaccgccc gttcccgacc
     3481 gtccagctct gcgtcctgtc tacggccgtc cccttcggca gcaccagaaa acgtccgttg
     3541 ctgtcttcca ggtacgtcac gccccaggtc ttcgcgttca gcttcgacag cgctgcgttc
     3601 tgccggtcga tttcggccag atttgacgct atcaagctcc cctgatacca caacaccccg
     3661 ctcaggctgg cgaacagcag tcctgacacc atcgtgatgg tcagccatgt ccgcccgacc
     3721 atacgcatca cgctcaggcg gttcgactgc atggccgcgc tcataccctg ctcgtgggcg
     3781 cggatggcgg cgctgattct cttcccgctt tcgctcagtt ccgctctgac agacttctcg
     3841 tgctcgctga atgcggcttt cagcatttcg ccggtactct gctgctgcgc tttcgatttc
     3901 tgctctaagt ccttcgccag cgttaaaaga ctgttcatag atggctccct taagtcggat
     3961 gtttcgccct ccgtccgggt cggcaatgct gatgctgctc tttgtggtgc gcacgacctc
     4021 aaaacctgcg ctttccagcg cctcagtgac gtcctgacgc gttttaagct cccctgacga
     4081 ggcgagggcc agtaagcccc gcgtaatagc ctctgcggct tcctgcttcg ttttgggtaa
     4141 cgaagatggc gtcaccagcg cgcgccggtt ctccggtgcg ttcgggtcgt gcagccccag
     4201 tcgcccgttc accacggtct gccacgcatc gatgcgcgga cggtctgccc ggtcgtagta
     4261 cggctggaga cgcctgccgg tcagcagttc cgtgttcggt accagaaaat tcagttccag
     4321 ccgccccttg tcccggtgtt caacccacag cacgctgtac tggtctttat cgagtccggg
     4381 catcagaacc cgctcgaagc tcgccatcag cttttcacgc tggccgggcg gtaaatcctg
     4441 ttcagcaaaa gacaggacgc cggaggtgta ctttttggcg tagggcgagg cgtcgataag
     4501 ctcccggacc tcctccggct ttccctgcag gacgatggcc ccgtcgcgct ggcggtcttt
     4561 ccccagcaga taatccaccg gcccggcacc gccgcctcgc cctcgcggat gaaacttaac
     4621 gatcatcgtc tgcccccttt tccagcacgg catgccgcag ccgctcgagt tcggcatcaa
     4681 tggccatcag cgcggccacc acctgaaccc ggtcagcgcc tgaccactgg ccgccgttaa
     4741 ttttccgggc gatctggttg aggttgttcc ccatcccggc aagctggcgc agcaaaaccg
     4801 gatcgatgga cggcagccgc aacgaccgcg ccgggcgcgt atcgagacag gtttcccgca
     4861 tccacaccgc cagttgcctt ccattgcagc gctccagcag ctgctggtac tccccatcgg
     4921 tcacccggat acctaaaaaa cgggtacgct taaccgccat cctctcctcc gatatccgca
     4981 aaatgtgacc gggctgaacg ctcaagctct gccatctgct cccggcggta atcttcctcc
     5041 tgtgcctgac gcatcagatt tgccatcact gccggatgcc aggatctttt ttttacgacg
     5101 agcggcttct tcttcggctt ctgcattatc cctccttcgc aaacgccgga cggcacggaa
     5161 aagttttccg ggtcgctccg gcgttggaca agcgccagcg cgtcagtggt gttagcgggt
     5221 ttcggggcgc agccctgaac cagtcacagc gtttacgctg tgtatactgg cttactatgt
     5281 tggcatgaga gcacgattca tgaaagtgcc tcatgtggca ggtgtgatat gtggctcagg
     5341 agcgtccgca ataccatgca cggacagatg aaatgacgcc tcctcgctca ctcggtagct
     5401 gcgctcctgc cgttcggctg cggcgggcgt gaccgactca cggataacgg gataaagcaa
     5461 aggcgctcaa atcagaaagg gccggagaaa aggatgcggc gttgccgttt ttccataggc
     5521 tccgcctccc ctgacgagca tcacagaaat ggacgctcaa gtcagaggag gacgaaaccc
     5581 gacaggactt aaagat
//
