LOCUS       NZ_CP092719            10061 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Klebsiella pneumoniae strain HvKp-su1 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP092719
VERSION     NZ_CP092719.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26245098
            Assembly: GCF_024299245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Cai,Z., Jia,T., Pu,M., Zhang,S., Zhang,J., Geng,R., Chen,S., Li,Y.,
            Fan,H., Tong,Y. and Qu,F.
  TITLE     Clinical and Molecular Analysis of ST11-K47 Carbapenem-Resistant
            Hypervirulent Klebsiella pneumoniae: A Strain Causing Liver Abscess
  JOURNAL   Pathogens 11 (6), 657 (2022)
   PUBMED   35745510
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Cai,Z., Jia,T., Pu,M., Zhang,S., Zhang,J., Geng,R., Chen,S., Li,Y.,
            Fan,H., Tong,Y. and Qu,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2022) The Clinical Laboratory, Aviation General
            Hospital of China Medical University, No. 3 Anwai Beiyuan Road,
            Chaoyang District, Beijing 100012, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. july-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 41.97x
            Sequencing Technology             :: PacBio RSII; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:24:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,370
            CDSs (with protein)               :: 5,370
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 54 of 129
            Pseudo Genes (incomplete)         :: 76 of 129
            Pseudo Genes (internal stop)      :: 13 of 129
            Pseudo Genes (multiple problems)  :: 14 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092719.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="HvKp-su1"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="China:Beijing"
                     /collection_date="2014-09-01"
     gene            complement(join(10048..10061,1..469))
                     /locus_tag="MKX18_RS28015"
                     /old_locus_tag="MKX18_28020"
     CDS             complement(join(10048..10061,1..469))
                     /locus_tag="MKX18_RS28015"
                     /old_locus_tag="MKX18_28020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_254841920.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRNGGI"
                     /name="replication-relaxation family protein"
     gene            complement(469..2433)
                     /locus_tag="MKX18_RS28020"
                     /old_locus_tag="MKX18_28025"
     CDS             complement(469..2433)
                     /locus_tag="MKX18_RS28020"
                     /old_locus_tag="MKX18_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3917..4066)
                     /locus_tag="MKX18_RS28025"
                     /old_locus_tag="MKX18_28030"
     CDS             complement(3917..4066)
                     /locus_tag="MKX18_RS28025"
                     /old_locus_tag="MKX18_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4151..4408)
                     /locus_tag="MKX18_RS28030"
                     /old_locus_tag="MKX18_28035"
     CDS             complement(4151..4408)
                     /locus_tag="MKX18_RS28030"
                     /old_locus_tag="MKX18_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(4418..6103)
                     /locus_tag="MKX18_RS28035"
                     /old_locus_tag="MKX18_28040"
     CDS             complement(4418..6103)
                     /locus_tag="MKX18_RS28035"
                     /old_locus_tag="MKX18_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6430..6675
                     /locus_tag="MKX18_RS28040"
                     /old_locus_tag="MKX18_28045"
     CDS             6430..6675
                     /locus_tag="MKX18_RS28040"
                     /old_locus_tag="MKX18_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6949..7320
                     /gene="tolA"
                     /locus_tag="MKX18_RS28045"
                     /old_locus_tag="MKX18_28050"
     CDS             6949..7320
                     /gene="tolA"
                     /locus_tag="MKX18_RS28045"
                     /old_locus_tag="MKX18_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            7317..7682
                     /locus_tag="MKX18_RS28050"
                     /old_locus_tag="MKX18_28055"
     CDS             7317..7682
                     /locus_tag="MKX18_RS28050"
                     /old_locus_tag="MKX18_28055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            7857..8135
                     /locus_tag="MKX18_RS28055"
                     /old_locus_tag="MKX18_28060"
     CDS             7857..8135
                     /locus_tag="MKX18_RS28055"
                     /old_locus_tag="MKX18_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            8123..8437
                     /locus_tag="MKX18_RS28060"
                     /old_locus_tag="MKX18_28065"
     CDS             8123..8437
                     /locus_tag="MKX18_RS28060"
                     /old_locus_tag="MKX18_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8601..9029
                     /locus_tag="MKX18_RS28065"
                     /old_locus_tag="MKX18_28070"
     CDS             8601..9029
                     /locus_tag="MKX18_RS28065"
                     /old_locus_tag="MKX18_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            9055..9234
                     /locus_tag="MKX18_RS28070"
                     /old_locus_tag="MKX18_28075"
     CDS             9055..9234
                     /locus_tag="MKX18_RS28070"
                     /old_locus_tag="MKX18_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9261..9791)
                     /locus_tag="MKX18_RS28075"
                     /old_locus_tag="MKX18_28080"
     CDS             complement(9261..9791)
                     /locus_tag="MKX18_RS28075"
                     /old_locus_tag="MKX18_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9798..10058)
                     /locus_tag="MKX18_RS28080"
                     /old_locus_tag="MKX18_28085"
     CDS             complement(9798..10058)
                     /locus_tag="MKX18_RS28080"
                     /old_locus_tag="MKX18_28085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228733410.1"
                     /translation="MAFEVERTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLV
                     RVQRAILAVDEIVNPQTGEARKTVELDRERLFACFKFMTTE"
                     /name="hypothetical protein"
     BGC             1..10061
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3236..3814
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..469)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.949"
                     /coverage="100.0"
                     /evalue="3.89e-81"
     Typing          complement(9802..10061)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.253"
                     /coverage="100.0"
                     /evalue="1.85e-46"
ORIGIN
        1 ttctgccgtc cagctccgcc agcgcgtccg gcactttcat ccctttcagg gccatacggt
       61 gcaggtaccg ccagccggtg gccccccggc cctccatgga gaggcgcagg gactgcacgg
      121 ccagccggtg cggcagctgc gccgccgaca cccttgacgg ctgaaacgga ataacgtccg
      181 tcagcggctc gtcctcgtcg aacgagaacg ccaccccgtg cggcgtcagc ccccacacgc
      241 tgacctgacg gccgtcaacc gggtatttcg cccggaccag cagccctttc gccaccagac
      301 tgtccaggaa gcggtaaacc ggctgtaccg ccgcaatccc cagccgtcgc gcaaccagct
      361 ccgccgtgga acacagctct tcccgcagcc agcgcagggt cagccgtgtt ttctccgcgt
      421 tccgcgcctg ccgcttcgcg tggctgacgt tgtactgctc cagtgtcatc agtaaagccc
      481 cgcaaaatca tcatccgctt ttggtccgcc gccgtcggcc ggaggcaggt actccgccag
      541 cgggtccgtt ttcgccagca ccgggaaggt tttcagcgaa atttcccggc gctcaacccg
      601 cagcgggctg ctgaacgcca gccgcgccac gcccagcccg gtcatcaccg cgaacccgtc
      661 aggcaggtgt tgcagggtgt tcacgtcaaa cagcggccgc gcctccttct ggatgaagct
      721 gtcgccgctg atgtgctcca cgttccccgc ctcccggctg gcccggcggc gctccacgtc
      781 cacccggatc tccccggtct ggcccgccgc ccactgcgcg gactcctggc tggccgcgcg
      841 atagaaccag cggatcggcg tgttatccag taccgtggtt ttcacaaaat cagcgggcag
      901 atcctgcccg cactgaccga agtcgcccag cgactggtgc gcgagcagca ggttgcagtc
      961 ccggctgcgc agcgtcccca gcgcgttcag cacgtacttc gacagcagat atttaatctc
     1021 atcgagcatg atgctcgcgt gcggccacct ccggaactca tcccgcgcaa tgatgatttg
     1081 cgcgcagcgg gcaaacagca tcttctgcac ccggatcacc gcctcatcgt ccatcgagcc
     1141 gatgacgtac aggcagccgc cgccgttgat tatccccgcc acatcgatac cgctgtccgt
     1201 ctgcaggacg ctcaggtcgg ccacgttctc cagcttggtg atcagcccct tcacgtcctt
     1261 tttcagggcc tccggcaacg cgtacgccgc cgccagcacc tgctgaatgt ttgccccctg
     1321 cgggaactgc tccgcaatca gcttcgccgc tttctgctcg ctgatgcggt agtggtccgc
     1381 cacgtcgccg gtctcactca ggttaaaccc ggcaaccagc agattcttca gggcgtactg
     1441 gtcgagatcg cggaacagat tcagctgcgc cggtttcccc ggccgcagat ccagcagggt
     1501 gaagtccggg cagtgcgccc ggaagacgtg cggcgcccac gcgtcccctt tggggtcgaa
     1561 gtacaccaca gcgtccccga aatcgcgtac gcagcgaatg agcgcgttgg tggccatcgc
     1621 gctcttgccg ctcccgggca ggccgaggat ctgaatattg gtcttgcgcc aggtatcacg
     1681 agggaccagc accggctggc cctgctcatc cttacccaga aacaccgcct gctgtttccg
     1741 ggcctgctcc gtggaaaaat cggccagcgc gttaaccggt accggctccc cgaaatgcag
     1801 gtggcggacg tctgtcattt ctccggtccg ctggctgctg cgccggctct cctgattcag
     1861 tttcgcgttc accgcctcgt cagcggccgc aaaatgacca tcaagcagca ggaacgccag
     1921 cagccgcgcc agaaggacgc cggcgacaag ccctccggca caccaggcta tcccgaacag
     1981 gttagccggc tgcagcaggt aataatctgc gttcgcatag aggtccgggc gcaggacggt
     2041 ttctccccag ctctcgataa acacggcgac aaccagcccc cccagaaggg ccagaccggc
     2101 agcgccggtc agccagtacg cgtagttcca gacatgtcgc cgtgccctga ccatccgccc
     2161 gggtgaatcc agcgaggcca gcaccgaaaa tggcacagtc cggttattat cgcgcttata
     2221 gcgatcggca tactcccgcc gcgccttatg gctcagcccc acaaaacgca ttcggccggc
     2281 ccagagttca gccagaccga aaaacaggcc gataaccaga aaaaaaccgg ccagactgta
     2341 atactcccgg tacagccagc tgacccacgg gtaaatctgc cgggccagct ccgggatatg
     2401 ccactgccag gcaagcgaat ccgtattaaa catgcaatct ctctccttca gacgacgggt
     2461 ttaccggcgt gatgcgcagc ccccgctgca caagccgcgg acatgcccca aggcatggcg
     2521 gcggcggtca cgtccgtggt gattgcgttc agctttcacc gcggaggcca gcctctttcc
     2581 gccccatgcg gaaaaggcgc agccccggag gaacaacgag cacgccccag cgtgtgagag
     2641 gaaaaggcgg caccccagtg cggtcttttc cggtgttccg gcccccgtca gagttcgcca
     2701 tagcgagcgg ctgaccaccc gcaaaatcag gagcagcttt gctgcttcgt caggtgcgtc
     2761 cagcaccgtg gatttttgtg ggtggtcagg cgtgagcctg acgggggccg ggtgaatttc
     2821 tggcgtggcc ggcgtccagc cggacagagc cagaaagagg ggaaaagacc gtgcgtcggt
     2881 ggcgtccagc cagcgtaagc aaggtctttt cccctgaagt cagccacgcc ccagcgggga
     2941 tgacttgcat ttctccgcaa ggagaaaaaa gcaacagaga taggttaacg taaaatgttc
     3001 agcgaaaaac cgacatggtt cacctatcct gataattgat cgtcagggaa tagaaagact
     3061 taatcagggg acaatatccc acatcagcgc gtccggtgac cgtcttgcag gtgccgggga
     3121 aattgcgcct cctcgctcac tcggtcgcta cgctccgggc gtgagactgc ggcgggcgct
     3181 gcggacacat acaaagttac ccacagattc cgtggataag caggggacta acatgtgagg
     3241 caaaacggca gggccgcgcc ggtggcgttt ttccataggc tccgccctcc tgccagagtt
     3301 cacataaaca gacgcttttt cggtgcatct gtgggagccg tgaggctcaa ccatgaatct
     3361 gacagtacgg gcgaaacccg acaggactta aagatcccca ccgtttccgg taggtcgctc
     3421 cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga tacctgtccg ccgttctccc
     3481 ttgcgggaag tgtggcgctt tctcatagct cacacactgg tatctcggct cggtgtaggt
     3541 cgttcgctcc aagctgggct gtaagcaaga actccccgtt cagcccgact gctgcgcctt
     3601 atccggtaac tgttcacttg agtccaaccc gggaaagcac gcaaaacgcc actggcagca
     3661 gccattggta actgggagtt cgcagaggat ttgtttagct aaacacgcgg ttgctcttga
     3721 agtgtgcgcc aaagtccggc tacactggaa ggacagattt ggttgctgtg ctctgcgaaa
     3781 gccagttacc acggttaagc agttccccaa ctgacttaac cttcgatcaa accacctccc
     3841 caggtggttt tttcgtttac agggcaaaag attacgcgca gaaaaaaagg atctcaagaa
     3901 gatcctttga tcttttctac tgaaccgcga tccccgtcag ttcagaggag gacgatggtg
     3961 ccaccgttcc gccctgaaca tcccggatat aatttgcctg acatgcggcc agaagaaagc
     4021 cagcaacaat caatataaaa agaaaaatag tctttacctt tttcatatac tttccctgta
     4081 tttaaagact ccggcattca tccctgaaca ccggaagtca gtatttcaaa tcatccttga
     4141 taattttttg ttaccagtta ccgtcccgat aatcaaaagc tatataacaa ttatccacat
     4201 caggttttat ttgattttta aaaaatggct gtagtaaagg aacccattct ttcttcaaat
     4261 caaactcacc attattaata ttatccttta aaggcattcc caagctttca atgacagaac
     4321 catcgtcacc aaagtcttta gaatactctt cccctttaaa ctcttcagtt ttcttatcaa
     4381 accatgtcaa acgtaattta agccccatat ttacctctca aagatatttt ttaatgttcc
     4441 gttttggatc tggcccttta acctgcttac ccgttttggg atcaaatgcg cctaggtgtt
     4501 cgccatcact ggcacgatac ccttcaagct caccgtgctg ggaatcccat tcataaattt
     4561 tacgtttttt atcgccatac catcgggccc gcttaccacc accaccttgt ttaggtgttt
     4621 taggatcacc tcgctttaac tctccaagtc cctttatgtc ttctgtttta ggtactggat
     4681 gataatcatg gccgtagtct ttagtcccct tgcgaggctt gttcttttcc tcatttaact
     4741 tattttctgc atccttcgct tttttctcct tttgtttccg gctctccatc gccccggtaa
     4801 gtgcagcctt atctctggat aaggattcat tcattgcgtt taaagatgcc tgagccgctt
     4861 ttaatttctc attctgattc tgaatatcct tatcaagata gcgaacctga tcctgccacc
     4921 cggagccata ttcatgaggc gggttataag tcacaaaatc atctttattt gcctctgcct
     4981 cttttacctt tttatcggct tcctgaacag cagcccttgc accggggagc gccttctctg
     5041 tcgatgcaat gcggctgttt aaggtcgcaa tatttttatc ttcggcatcc agctcagctt
     5101 tcgctttatc atagtctctt ttcagcccct cttccggatg agcggcgtcc catgcctgct
     5161 ggcgacgctt ttcttcctcc tgacgctgtt ttacctgtgc cggggtaaga acatctgtca
     5221 ccgacacata aaccggcttc tgtccggtct ctttcgggaa acgaataacg gcctcacggg
     5281 agttaccacc ggctgtaaaa ccagccggac gtgaatcacc tttttcagca acaatgcctt
     5341 ttggcggggc tttcgctgtc ggaacacctt tcggtacgct cacctgcaga gacgggagac
     5401 ccggaatcga cacactgaat acccccggac gttttgtcgg tttcgcatcc accacaggga
     5461 cactcattgg ccgacctgcg acaaccgcaa tatgctgccg ctcatccttc accacatcca
     5521 caacccgttg tctgacacga accgtcgcct ggtccagtgg taaagtactt gccggagtct
     5581 ccgtcaccgc atcggccggt aatgacgtca taatttttga catcattttc ggatcatctt
     5641 ttgcaatctc agaaggcagc acaccgtaga tcgcaatccc ccacagacca aacttaaacg
     5701 gccccttcag ggcagccagc acatcagcaa cggcggccga caacgcatcg ccggaaactg
     5761 ataaagccag tccttcagcc cccggagtag ccagggcagg cagaccgaag gccatcgcag
     5821 tggccgaaga ctgtccgcca ccagagctac cgctatgatt gccgctgtta ccaccattac
     5881 caccgggttt actcccgccg ttaccaaact cccccggatt atagttatga atatctccat
     5941 tcggcgtatg cgtcgtaccc cagcccggcc cgctgtttgg atccgtacca ccacctgatg
     6001 aagggccacc tttaccagac gtaccgttca cattcccact ggcctgacca ccattatgtc
     6061 ccagacctga attacccgga cctcgaccgt ctccaccact cataccgttt tcctctttga
     6121 catttaaaac aataaattaa agccaacata ctgtacatat acacagtata attattacct
     6181 tactcctccc gttttcaggt gtcaatatat gagattagtt ctcatcttca ggcacaaaaa
     6241 agcgcccgga gccacctgta agcagatcgc gtcttttccc gaaaatttct catggcaaca
     6301 aattttatta tttaagacat tcatcacact tacatttcag atggtataga cacatctgaa
     6361 agctaactgg catgaggaga ggacctgcgg ttaaactgtt cgggaaacct gagacgcccg
     6421 gaggccacta tgacgcacct gaaactgact cccgctgaag aaaagcgcct ggcggacgcc
     6481 tttgtcgctc aggcactggc catgggcctg aaagggaaca aaacgctgcc aaaggcggta
     6541 aaacaacagg tgcgggaaag ggtgcttcgt gccattcagg acgaacgtga agccaggcgg
     6601 gcgcaggaaa tgcgtcggga gaaatacgag gctacggaaa ccaccttcac ctggcagcca
     6661 cagcgccgcc ggtaaggggt atttaccata acgtcgcatt gcccgcaccg ctcagacggt
     6721 ctgagggcgt ttccggcggc tacgggataa aacaggcaac aaaccacccg gaaaaccgcc
     6781 ggaaacgcgc ccggtaaatt gtaacccgct gcatgcctat gcacgcaact gcatggcggg
     6841 ccgtttggtg taaaaacgat acgataagcc gtgtttatgt aaatgaacgt ggccgtaaag
     6901 agtgacaaat atacatgtaa cattttttac tgacaggtca tcaacccgat gcgtttaata
     6961 ccctttatcc tgctggcatt gtgcagcccc ttactcctga cggcctgtaa gcattccacc
     7021 acccagccgc cgaaaattta tactctggat aagtcatcta aaatcagtag ctacgctgcg
     7081 gccatcaaga ccgctgttga aatgcagatg tttgaaccag aggcctatgc gggaaaaatg
     7141 tgcaccgtgc ggatatccat acaacgtgat ggcactctga acagcgccat tgctgaagga
     7201 ggagatccga aactctgtaa agcagcaata tcagccgtta cccacgccaa aattccgccg
     7261 gcaccggatg aaaatacctg gcaaatattc aaaaacgccc ctctggattt cagaccatga
     7321 aagcttttac tctgacagtg ctggcggcca tgttcctcac tccggtacat gcgataccac
     7381 taaaacccgt aaccacactg cagccaacct acccggtaca ggccgcagca aaacgcatcg
     7441 aaggtcaggt gaccgccacg tttgatattc tggacgatgg acgagtggac aacgtgtcgc
     7501 ttaccggctg tcaccttttt tttcgggaaa cgctgacggc aatgaggcac tggctatatc
     7561 ccgcagggca cccggcaatg cgacaggtta taaattttga attcagtctg aaaaatgacg
     7621 gcttacagga tccgcgacca gtcaccaccc aaaagctcaa tgatgatggt tgccgccagt
     7681 aacaggtcct ttaacccgca ggctgcaata gtcctggatg atgagtgagc ctgtagcagc
     7741 cctgattagt ggaagcgaag ctgaaaaatg ggcagcaaaa gccgtatttc tggcaaatag
     7801 cgccgccacg gattaaagta gtcatgtgta atacatatga ctacaaaggg gcttttatgt
     7861 catcagcaac atccatcagg ctcgatgaag aacttaagga ccggctgaaa acgctggccg
     7921 atgaccggca ccgctcagcg cacgcgctaa tgctggaggc aatcaccgaa tacatcgacc
     7981 gggaagagaa gcgcagccag tacctgcggg acggacaggc cgcatggcag cactatcagg
     8041 aaacgggtct gcaccttact gcggaagaag cggaagcctg gataagtacc tggggaactg
     8101 agaacgagca ggatgctccg ccatgccaca ggtaacgatt tccgcgctgg cacagcgcga
     8161 tttacaacgc cttcaggact ttctcaaaac caaaaatcgg ctggcagcca gaaaaggagg
     8221 tgaggtgatc gtccgggcta tccagcaact gaaaacattg ccagacattg gccgcccggt
     8281 gccgtttctg ccgctggaat ataaggaact ggtgatcggg tttggcgaca gtggctatgt
     8341 gatgctctac cgccacgacc gggaaatgga ccagattgtg atcgtcaccg tcaggcatca
     8401 gaaagaatcc gggtatccgg gggcagacag cctctaataa ccgatgacgg gggcgcaatt
     8461 cattgcgtgg ccgtcgccgg tttgacaagc gcagcgcgtc agaaaaccac atcctgaacg
     8521 gaaaaacggg taaaattcag cgatacattt accgtgagaa attacacacc tttcacaccc
     8581 gataaaatca ggggtataca gtggcgaaaa cattgctcaa cctttcacag gcagcacaag
     8641 ccgcgggcat taccagaaga acgctataca atcatgtaaa acagggaaag gttaccgtgt
     8701 cacgtgacgg gaaaaacaac cctgtcgtgg atgtgtcaga actgatccgt gtatacggaa
     8761 atgtgaatat ccctgagaaa caaattcccg gcatttcaca ccgtgaaaat acacaaaaaa
     8821 atttcccaca ggaacagctt ctggcaatgc aaaaggaact ggccgatctc cggcaggctg
     8881 tcaccctgat gctggaagat aaaacatccc gggaagaaga acgccgccag cacgatgatg
     8941 agcgccggaa actccaggcc gaagtggaca gattgacaac agaactgaca cagaagaaaa
     9001 aacgcttctg gtccggctgg tttagctgag aacttaccga agggaatgga gaacatgacg
     9061 aagcaggaaa aaactgccct caatatggct aaattcatta aggatcagtc gctgctgttg
     9121 ctggaaaagc tgaacgagct ggacatggat accccggccg acatgtgcga acggcttcac
     9181 gaggatgcgg aaacactctg gcaggtgatg cgggataagc tcggggaaga ataacagcac
     9241 cggccatgcc ggtgctggcg ttattttttg ccgaacggcc accaccggcg ggcgggtttg
     9301 tcctccggca gttcaggcac cggcacgtta aaatagcggc tgttatattc ccgcatcagc
     9361 tccgctttca gcttctcgtt caccgcgctt tccccgtgcc gctcgctgac cacctcaatc
     9421 atcgcgttaa tctgctccgg tgagcacttc ccggcatcaa ccacctgcac accgttctcg
     9481 atggcgaacc gctccacctt tcgatagagg taatcacaca gcttcattcc tgtgggtttg
     9541 tcccgttccc attgcggatt ttcaccggtg aaaaagtacg tggctttttc gtcgctgttg
     9601 agccgctcgt catacatccc gatggatttc atcatcgcca gatgctgggc ccggtcgaca
     9661 aaacagaaaa catacgcccc ggtctgccag gcgagcacca ccatgtcttc cagatgctcg
     9721 ctggttggca taatgcagcg ctcgccgtct ttgtcttcca cgtcctcaat aaataccatg
     9781 tacagcgcca taatccctca ctccgttgtc ataaatttaa agcacgcgaa cagcctctcc
     9841 cggtccagct ccaccgtttt gcgggcctcg ccggtctgtg gattcacgat ttcatccacc
     9901 gccagaatcg cccgctgtac ccggacaagc gtcggccggt cagggcagat gtaccagatt
     9961 tcatcgatgc cgttcgcccg gcggttgaac agatacccgg acaccacttc ctgataccgc
    10021 ctgcggctct tgacggtgcg ctctacctca aatgccaccg t
//
