LOCUS       NZ_CP092651             9730 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KP7728 plasmid pKP7728-3, complete
            sequence.
ACCESSION   NZ_CP092651
VERSION     NZ_CP092651.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26106931
            Assembly: GCF_022453565.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Huang,J. and Li,X.
  TITLE     Multiple CRE strains carrying NDM-5 gene were isolated from the same
            patient during hospitalization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Huang,J. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2022) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue Street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022453565.1-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 04:14:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,233
            CDSs (total)                      :: 5,106
            Genes (coding)                    :: 5,000
            CDSs (with protein)               :: 5,000
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 43 of 106
            Pseudo Genes (incomplete)         :: 64 of 106
            Pseudo Genes (internal stop)      :: 13 of 106
            Pseudo Genes (multiple problems)  :: 13 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092651.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP7728"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP7728-3"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2021-07-17"
     gene            join(9329..9730,1..330)
                     /gene="mobC"
                     /locus_tag="MJ615_RS26090"
     CDS             join(9329..9730,1..330)
                     /gene="mobC"
                     /locus_tag="MJ615_RS26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            337..867
                     /locus_tag="MJ615_RS26095"
     CDS             337..867
                     /locus_tag="MJ615_RS26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(895..1074)
                     /locus_tag="MJ615_RS26100"
     CDS             complement(895..1074)
                     /locus_tag="MJ615_RS26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1143..1520
                     /locus_tag="MJ615_RS26105"
     CDS             1143..1520
                     /locus_tag="MJ615_RS26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(1582..1881)
                     /locus_tag="MJ615_RS26110"
     CDS             complement(1582..1881)
                     /locus_tag="MJ615_RS26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1871..2134)
                     /locus_tag="MJ615_RS26115"
     CDS             complement(1871..2134)
                     /locus_tag="MJ615_RS26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            2511..3596
                     /locus_tag="MJ615_RS26120"
     CDS             2511..3596
                     /locus_tag="MJ615_RS26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            3593..5782
                     /locus_tag="MJ615_RS26125"
     CDS             3593..5782
                     /locus_tag="MJ615_RS26125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_240289463.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTADGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            7368..9329
                     /locus_tag="MJ615_RS26130"
     CDS             7368..9329
                     /locus_tag="MJ615_RS26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     Typing          complement(5988..6568)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.454"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5..327
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.222"
                     /coverage="99.0"
                     /evalue="8.49e-64"
     Typing          9330..9730
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.522"
                     /coverage="99.0"
                     /evalue="3.5499999999999997e-68"
ORIGIN
        1 tacctgcacc gtatggccct gaaggggatg aaggtgccgg acgcgctggc ggagctggac
       61 ggcagaacgg tggcgtttga ggtggagcgc accgtgaaga gccgcaggcg ctatcaggag
      121 gtggtgtccg ggtatctgtt caatcgcaaa gcgaacggca tcgatgaaat ctggtacatc
      181 tgcccggacc gggcgacgca ggtcagggtg cagcgggcga tcctgtcggt ggatgaaatc
      241 gtgaatccac agaccggcga ggcccgcaaa acggcggagc tggaccggga gcggctgttt
      301 gcgtgcttta aatttatgac aacggagtga gggattatgg cgctgtacat ggtatttatt
      361 gaagacgcgg aggaccggga cggcgagcgc tgcatcatgc cgaccagcga gcatcttgaa
      421 gatatggtgg tgctcgcctg gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc
      481 cagcatctgg cgatgatgaa atccatcggg atgtatgacg agcggctcaa cagcgacgaa
      541 aaagccacgt actttttcac cggtaaaaat ccgcaatggg aacgggacaa acccgcagga
      601 atgaagctgt gtgattacct ctatcgcaag gtggagcggt tcgccatcga gaacggtgtg
      661 caggtggttg atgccgggaa gtgctcaccg gagcagatta gcgcgatgat tgaggtggtc
      721 agcgagcggc acggggaaag cgcggtgaac gagaagctga aagcggagct gatgcgggaa
      781 tataacagcc gctattttaa cgtgccggtg cctgaactgc cggaggacaa acccgcccgc
      841 cggtggtggc cgttcggcaa aaaataaacg gcagcaccgg ccatgccggt gctgttattc
      901 cttcccgagc ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt gcagccgctc
      961 gcacatgtcg gccggggtat ccatatccag ctcgttcagt ttctcgagta acagcagcga
     1021 ctggtcctta ataaatttag ccatattgag ggcagttttt tcctgcttag tcatgttttc
     1081 tgtccttccg ggtgtggtgt gcttcctgtc gggtatactg tctcaaaacc ggaggtgaga
     1141 caatggcgtg ggtgacggtg agacaggcga cggaactgac ggggaaagcc cggagttccc
     1201 tgtataggga tatggcgaaa ggccgcgtat cataccgtac tgaagctgat ggtggtcggg
     1261 tggtggatac cagcgaactt atccgggtgt acggtgaatt aagacagatt gagactcacg
     1321 agcgggacgg gttgagactg tcggatgaga ctgaaaaaat ggcctccgac gcgttgatcg
     1381 cggagataaa agcactcagg gaggaggtgg ccggactgcg gcaggaaatg caggccatga
     1441 gactgctgga acacaaacca gagacagcag agacaccggc cggaacatcg cgccgatggt
     1501 ggcagtgggg aaaacgctga cgcgctgtgc ttgtccaacc ggcgacggcc acgcaatgaa
     1561 ttgcgccccc atcaccggtt atcagcgaac ggtatgggga ttctggcgct ggccaaccac
     1621 ggccagaacc cggactttcc tggtctggtc atcaacatca tagaggacat gctgtcccat
     1681 cagactgatc cggcgaacgt cctcgccata gcgtgagtcc acttcatata ggcgcgggaa
     1741 gtcggccagt ttgtccgctg cagcgagaac tcgatcggta acgttctgcg catcccacag
     1801 ggatgaattt tccgcaatgt agtcaaacaa tgctgtcaca tcctcgatgg ctccctgctt
     1861 ccattccacc tcatacggca tcgcgtagtt cctgatagcg gccatgcagt ctctggcgga
     1921 gttgttccgt ggtcatgccc gggcgcgtgt cagcgcgggc ggcggtcact ttagcgcgaa
     1981 cctgatcttc atattctgcc tgggccagtt cttcgttagc caggcgctgc aacagtgcag
     2041 ctttgtccgg gccaccttca ggcagtatgc ggggcagtct ctcggccagt tcccgcagga
     2101 aattggcgtt ttcatagtgt gtactgttgc tcattgtgga gcctcccggg ctgatacgtc
     2161 gatatgaagg agtttagcag ggggcggtct gaaagaatat gctcgtgtca gtcaggctga
     2221 tgaagacgaa agctggtaaa aaagggctta cgacacccat tttacgtcaa actgcacgac
     2281 atgcagttgc gtgcatagcc atgcagcggg ttaaaattta tcaggcgcgt ttccggcggt
     2341 tttccgggtg gtttgttgcc ggttttatcc cgtagccgcc ggaaatgccc tcagaccgtc
     2401 tgagcggtgc gcgtaatgcg acgttatggt aaatcatgtg attaatggta gtgataatca
     2461 ttatcacttt tgctatattc ttgtttcttt tattgtaagg aaatgtaagt atggcaatca
     2521 cgattaaatc cgccgttaat gtccgtagca gagtattaat atactcttta ctactgttta
     2581 ctggttcagc gtatgcggct gatatagctg aatcaacatt cctgactcgt gatatcggca
     2641 atggtctgta tgaaatgtct tttgatggca aaaataatag tctctgggca gcatcatcgt
     2701 cttcattcga cagagataag ggccacggaa ttgtttatca gttagaacca tccactctga
     2761 aaacaaaatc tgccgttaaa atcgcacgtc ttgggtttgc cacagcactg gatgaggaac
     2821 atgagcgact ttatatcggc aatactctgg acggctcagt gacggtcatt gatactctaa
     2881 ccggaaaaga actttcagta attccgttaa ctgagggaca ggatgaggct catttcagac
     2941 cgaccagaga aatggtcatt gacaaaaaaa atcagcgtct ttatgtctcc agcgccgcag
     3001 ataaaggggc tctgtgggtt attgacatgg ctgtcgggaa gaaaattaaa accattgatg
     3061 gactggggca cgctgttact ggaattgcgt taaataaaaa tggggataat atttatctgg
     3121 ttaacgggga tggtgagatt attaacctgg atgccagtag ctatgaaata aaaaaacggt
     3181 ttgttgtaga gcctgagaaa aaacactttt tccttaatgt aagtctggat gaaaaaaacg
     3241 gacgtgcatt tattaccgac ccggatattc ccggggtgct ggttgttgat gtaaactcgg
     3301 gaaaaatact acacagaatt gatgttataa attcactggc gattttattt aatcctttac
     3361 ggaacgaaat ctatatcacg caccgtaatg caaaacaggt cagcattgtg gacagtaatt
     3421 cttattacgt aaaaacaacg ataaaaaccc gcctgatgcc aaacagtctg tctttatcac
     3481 atgacggtga aaatctgtac gttagtgtaa aacaagaaaa aaaagagatg agtactatga
     3541 aggattacat actcaaaatc gaattatcta aaattgctat ggagccagaa gaatgaataa
     3601 atatttaggg ctgagcatag tatatatgat gtatacatca tctgttatgg ctgatgattc
     3661 tggcgcagtt agtaaagata aaaatatttc accagaagaa acattaattg ttcatgggac
     3721 cgctgtttct gaaagcacaa cagaaggaac agattcatac acaacccagt cagccaatac
     3781 agcatcaaag atgaatctgt cattccggga tacacctcag tcaactacag tcattacgcg
     3841 acgaagaatg gatgatgtag cagcaaaaaa tattattgat gtggttaaaa ccgctcctgg
     3901 attatatgtc agtacagcag acggttccgg aagaccgaat tttatgtcca gaggatttaa
     3961 tgctaatgtc atgtatgaag gatttagcct gccgtggaat tcatacattc caagttcaca
     4021 aactagttca gctatttttg accgggtgga gatagtccgc ggggcaacag gtcttgttca
     4081 gggtgccgga gacccttcag ccgcgataaa tatgatacac aagcgtccga cccggcaata
     4141 tcaggccagc actaacatcg gtgccggacg gtggaatgac taccggggtg gggttgatgt
     4201 atcaggacca ctgaatgaca gcgcgacctt acgcggaagg gcagttgtgg atggcgaaaa
     4261 cagtgagacc ttcagggata gtgagaaacg caggaataac gtcatttacg gaattatgga
     4321 ggcagatctg actgataata ccctacttac gattggggga tatcagcagt atgaatataa
     4381 caatcattgg tggtatgact tacctatttc tggtaccgga caccacctta atcttcctcg
     4441 ctcgacgttt acaggcaatg actgggagtt tgaaagaagt aaaagtaaaa ctgcatttgc
     4501 aactcttgag cagaagttca gtaatagctg gaagctgaaa ttttcaacgc tgcaaaactg
     4561 gagaaatgct gacctgttag ggacggcaac ataccggcat aaagataccc gacaaagtat
     4621 gtatcagcaa gtatggggat cactgaggga atataattcc aaaagctatg acctgtcgct
     4681 taatggggac tatgctttat ttggtcgtgt tcatcaattc gtggctggaa caactcagca
     4741 aaaatacacc gataaagata caggttatac atattcccca atatatattg agaatgttaa
     4801 tatatttgat catggttcac attcttcgaa aaaacccatg aagatgtcag gtgatgcatc
     4861 ctacgacgtc actgaacaaa aaagcgccta cgcagcaact cattttctac ttagtgataa
     4921 ctggaaaata ttgctgggtg gccgtcttga ctggtatgaa tatacaagta aaggtacatc
     4981 cattaataca ggttataaag ttaccagaaa tatcaccaaa tatgcaggtt caacatacaa
     5041 tcttgacgat catcatacca tttatgggag ctataccgat atattcaatc cacagtcaag
     5101 caccgacctc aatggtaaag tgatcaagcc tattgttggt aagaattatg agacaggtat
     5161 taagggtgag tattttaacg gcttgcttaa tgcctctctg gcggtatttc aggtagacca
     5221 ggaaaatcga gccaggctgc ttcccgatca gacagggtgt gttactaatg gcaatcctca
     5281 atgctatgag gctagcggta aagtacgcac aaaagggata gatttcgaac ttcagggcat
     5341 ggttacgcca ctctgggaaa ttggggtggg atatacatat gcagatacgc gatatataaa
     5401 agatggagat cgtagtaatg aaggcaagcg attcaatact gccgtgtcgc cacaaaattt
     5461 atttaagatt tcgagcatgt acacctttga cggaaaatta acaaactggc gagcaggagt
     5521 aaacacgtac tggcaaagta aaatatatgc taacataaca gacacaaggg atggcaatcc
     5581 ggttattaat aaacaaaagg cttattcaac aactgacatt attattggct atactccaat
     5641 gccacaactg gatttacagc tcaatataac taatgtcttt gataaggttt attatacatc
     5701 cataggtgaa tcgattggat atggcacgga aacatatgga gaaccaagaa atgttaaaat
     5761 tagtgcaacc tatagattct aaccatgcgg attgggtctt ctcctcggat ttgcccctat
     5821 aaatccgtct tttgtggttc cggcgtacaa ccccctgttt gccacttcag ttgatggtta
     5881 atcaaatcat gtagaggatc ttcttgagat cctccatggt tcgtcgtaat cttttgccct
     5941 gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag gttaagtcag ttagggaact
     6001 gcttaaccgt ggtaactggc tttcgcagag cacagcaacc aaatctgtcc ttccagtgta
     6061 gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg
     6121 aactcccagt taccaatggc tgctgccagt ggcgtttttg cgtgtctttc cgggttggac
     6181 tcaagtgaac agttaccgga taaggcgcag cagtcgggct gaacggggag ttcttgctta
     6241 cagcccagct tggagcgaac gacctacacc gagccgagat accagtgtgt gagctatgag
     6301 aaagcgccac acttcccgta agggagaaag gcggaacagg tatccggtaa acggcagggt
     6361 cggaacagga gagcgcaaga gggagcgacc cgccggaaac ggtggggatc tttaagtcct
     6421 gtcgggtttc gcccgtactg tcagattcat ggttgagcct cacggctccc acagatgcac
     6481 cgaaaaagcg tctgtttatg tgaattctgg caggagggcg aagcctatgg aaaaacgcca
     6541 ccggcgcggc cctgccgttt tgcctcacat gttagtcccc tgcttatcca cggaatctgt
     6601 gggtaacttt gcgagagtta gcagcgcccg ccgcagtctc acggccggag cgtagcgact
     6661 gagtgaggga ggaggcgcaa tatcccccct cccgcagacg gccaccggac gcgcggatgt
     6721 gacatattgc accgtgttac cgcctttccc ttccctgccg ataaatcatt cagataggtg
     6781 aagtctgtcg gttttctgct gaatatttta cgttaaccta tctctgttgc ttttttctcc
     6841 ttgcggagaa atgcaggtca tccccgctgg ggcgtggctg acttcaggag gaaatcccct
     6901 gcttacgctg gctggacgcc accgacgcac ggtctttccc cctctttctg gctcagctcg
     6961 gctggacgcc ggccacgcca gaaattcacc cggcccccgt caggctcacg cctgaccacc
     7021 cacaaaaacc cacggtgctg aacgcacctg acgaagcagc aaagctgctc ctgattttgc
     7081 gggtggtcag ccgctcgcta tggcgaactc tgacgggggc cggaacaccg gaaaagaccg
     7141 cactggggtg ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc ctccggggct
     7201 gcgccttttc cgcttggggc ggaaagaggc tggcctccgt ggtgaaagct gaatgcaatc
     7261 accacggacg tgaccgccgc cgccatgcct cggggcatgt ccgcggcttg tgcagcaggg
     7321 gctgcacacc acgccggtaa acccgtcgtc tgaaggagag agattgcatg tttaatacgg
     7381 attcgcttgc ctggcagtgg catatcccgg agctggcccg gcagatttac ccgtgggtca
     7441 gctggctgta cagggagtat tacggtctgg ccggggcttt cctggttatc ggcctgtttt
     7501 tcggtctggc tgaactctgg gccggacgga tgcgttttgt ggggctgagt cataaggagc
     7561 gacgggagta tgccgatcgc tataagcgcg ataataaccg gactgcgcca ttttcggtgc
     7621 tggcttcgct ggattcgccc gggcggatgg tcagggcacg gcggcatgtc tggaactacg
     7681 cgtactggct gaccggcgct gccggtctgg cccttctggg ggggctggtt gtcgccgtgt
     7741 ttatcgagag ctggggagaa accgtcctgc gtccggacct ctacgcgaac gcagattatt
     7801 acctgctgca gccggctaac ctgttcggga ttgtctggtg tgccggaggg cttgtcgccg
     7861 gcatccttct ggcgcggctg ctggcatttc tgctgcttga cggtcatttt gcggccgctg
     7921 acgaggcggt gaatgcgaaa ctgaaccagg agagccggcg cagcagccag cggaccggag
     7981 aaatgaccga cgtccgccac ctgcatttcg gggagccggt accggttaac gcgctggccg
     8041 atttttccac agagcaggcc cggaaacagc aggcggtgtt tctgggtaag gatgagcagg
     8101 gccagccggt gctggtcccg cgtgatacct ggcgcaagac caatattcag atcctcggcc
     8161 tgcccgggag cggcaagagc gtgatgggca ccaacgcgct cattcgctgc gtacgcgatt
     8221 tcggggacgc ggtggtgtac ttcgacccca aaggggacgc gtgggcgccg cacgtcttcc
     8281 gggcgcactg cccggacttc accctgctgg atctgcggcc ggggaaaccg gcgcagctga
     8341 atctgtttcg cgatctcgac cagtacgccc tgaagaatct gctggttgcc gggtttaacc
     8401 tgagtgagac cggcgacgtg gcggaccact accgcatcag cgagcagaaa gcggcgaagc
     8461 tgattgcgga gcagttcccg cagggggcaa acattcagca ggtgctggcg gcggcatacg
     8521 cgctgccgga ggccctgaaa aaggacgtga aggggctgat caccaagctg gagaacgtgg
     8581 ccgacctgag cgtcctgcag acggacagcg gcatcgacgt ggcggggata atcaacggcg
     8641 gcggctgcct gtacgtcatc ggctcgatgg acgatgaggc ggtgatccgg gtgcagaaga
     8701 tgctgtttgc ccgctgcgcg caaatcatca ttgcgcggga tgagttccgg aggtggccgc
     8761 acgcgagcat catgctcgat gagattaaat acctgctgtc gaagtacgtg ctgaacgcgc
     8821 tggggacgct gcgcagtcgg gactgcaacc tgctgctcgc gcaccagtcg ctgggcgact
     8881 tcgggcagtg cggccaggat ctgcccgctg agtttgtgaa aaccacggtg ctggataaca
     8941 cgccgatccg ctggttctac cgggcggcca gccaggagtc cgcgcagtgg gcggcgggcc
     9001 agaccgggga aatccgggtg gacgtggagc gccgccgggc cagccgggag gcggggaacg
     9061 tggagcatat cagcggggac agcttcattc agaaggaggc gcggccgctg tttgacgtga
     9121 acaccctgca gcacctgcct gacgggtttg cggtgatgac cgggctgggc gtggcccgga
     9181 tggcgttcag cagcccgctg cgggttgaac gccgggaaat cccgctgcag cgcttcccgg
     9241 tgctggcgaa aacggacccg ctggcggagt accagccgga ggccgggcgg cagcggccgg
     9301 gtgatgatga ttttgcgggg ctgtactgat ggcactggag cgatacaacg tcagccacgc
     9361 gaagcggcag gcgcggaacg cggagaaaac ccggctgacc ctgcgctggc tgcgggaaga
     9421 gctgtgttcc acggcggagc tggttgcgcg acggctgggg attgcggcgg tgcagccggt
     9481 ttaccgcttc ctggacagcc tggtggcgaa agggctgctg gtccgggcga aatacccggt
     9541 tgacggccgt caggtcagcg tgtggggact gacgccgcac ggggtggcgt tctcgttcga
     9601 cgaggacgaa ccgctgacgg acgttattcc gtttcagccg tcaagggtgt cggcggcgca
     9661 gctgccgcac cggctggccg tgcagtccct gcgtctcgcc atggaggccc ggggggcctc
     9721 cggctggcgg
//
