LOCUS       NZ_CP092640             3367 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Escherichia coli strain HS plasmid pIMVS1_EHS, complete sequence.
ACCESSION   NZ_CP092640
VERSION     NZ_CP092640.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26036044
            Assembly: GCF_022453605.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Mores,C.R., Aberg,M., Zund,M., Field,C., Slack,E.W. and Sunagawa,S.
  TITLE     Genome sequences of isolates from synthetic gut bacterial
            communities for functional microbiome research in mouse models
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Mores,C.R., Aberg,M., Zund,M., Field,C., Slack,E.W. and Sunagawa,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2022) Department of Biology, ETH Zurich,
            Vladimir-Prelog-Weg 4, Zurich, Zurich 8093, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 65.5x
            Sequencing Technology :: Illumina iSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022453605.1-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 04:10:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,422
            CDSs (total)                      :: 4,305
            Genes (coding)                    :: 4,140
            CDSs (with protein)               :: 4,140
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 55 of 165
            Pseudo Genes (incomplete)         :: 100 of 165
            Pseudo Genes (internal stop)      :: 36 of 165
            Pseudo Genes (multiple problems)  :: 26 of 165
            Pseudo Genes (short protein)      :: 1 of 165
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092640.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="HS"
                     /db_xref="taxon:562"
                     /plasmid="pIMVS1_EHS"
                     /geo_loc_name="Switzerland: Zurich"
     gene            942..1460
                     /locus_tag="MJ392_RS21990"
                     /old_locus_tag="MJ392_21990"
     CDS             942..1460
                     /locus_tag="MJ392_RS21990"
                     /old_locus_tag="MJ392_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000116157.1"
                     /translation="MSVRAQRAGKRNQSERRIELINTLWEGEEIETWDRNDRPRNDGFI
                     TVPRYLPLLGVLMDELSKGSPLSSTYLALWFRGSDEGLIEIRDKTVLALESGFASARGV
                     TTWTGRMRKLKELGFISCREGSSGEFHNVLIVHPLVAVKKLLDEGKITKGKTYNTFAER
                     VIEVKSSWE"
                     /name="hypothetical protein"
     gene            1464..1958
                     /locus_tag="MJ392_RS21995"
                     /old_locus_tag="MJ392_21995"
     CDS             1464..1958
                     /locus_tag="MJ392_RS21995"
                     /old_locus_tag="MJ392_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061357548.1"
                     /translation="MAGTVWNDVNTGREVFSMTHLHPQLHKVEVSGVLVEIEISFGFHV
                     FTDQKQVGKVMSFRGETRHFCQERYEGSKTIVQRILSAIENGEYITAFISKGQGQRYYH
                     LNHHDDFILMEIRKPQGKDNCLRIHVVTAYTMDEWGVVNKGRNLKFRYVLEQRLQGKKI
                     V"
                     /name="hypothetical protein"
     gene            complement(2299..2460)
                     /locus_tag="MJ392_RS22000"
                     /old_locus_tag="MJ392_22000"
     CDS             complement(2299..2460)
                     /locus_tag="MJ392_RS22000"
                     /old_locus_tag="MJ392_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045343414.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            3028..3177
                     /locus_tag="MJ392_RS22005"
                     /old_locus_tag="MJ392_22005"
     CDS             3028..3177
                     /locus_tag="MJ392_RS22005"
                     /old_locus_tag="MJ392_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_163396971.1"
                     /translation="MPSEYDEQRDATDALLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          2822..2994
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.0"
                     /coverage="100.0"
                     /evalue="2.04e-62"
     Typing          complement(2344..2897)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.369"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaataatc aaccatggag ggtgatttac gcaggttaag gggagatcgt gacaaaaaaa
       61 gcgttcctca ctgtatcgtt catgcctgca tgaacgggtt attcttcctg actacagaat
      121 tacatcaaaa gtacatcaaa attacatcac ttttacgtca gatcgcgggt gtacaacaga
      181 attacatcaa aagtacatca aaagtacatc aaaattacat cacttttacg tcacttttac
      241 atcattttta cgtcagatct gacctctgaa ctattcgtac atccgcccct cttcctgggc
      301 ctcctgcacg tgctgaaaat ggtttttccg gctgtgccgg caacgcctca cggccacgca
      361 ataaattgcg catccgttca ccgttatcgg ggtggttcgt ggtggctttc agggcgttca
      421 tcggatttta gccgctaaaa tcccggccag gtatgtccgt gcatagccat gcagtcgcgc
      481 cagaatcatc aggggcgttt tttctcgata tgggcacaga aaggtgtgtg tttcaggccg
      541 gaggcctcag aaacgcgctg agggcctctg cggcggtgca cgcaacggat gttatggtaa
      601 atgcgagtaa ccagcatggt gactcttaat ttatttttcg atttctcccc ccctccttgc
      661 cccctacaac cccatggagc acaagggagc accagaagca catggagcac gatgagcaca
      721 tggagcaccg caattaaata aaaactcttt ttaaatcata taaatgcatt ttgtgtttgt
      781 aatatttatt acttgtaacc gccttttgtg taatgaatat tgcatttttt atgattaaaa
      841 aaaataaaaa ccggaagtaa ttgttcaagg gaatttataa aagttattga tttataaggt
      901 ttttttggta aatttctctc acacattaac aggaggcgag tatgtcagtc agggcacaaa
      961 gagcagggaa acgtaaccag tctgaaagaa gaatcgaact gataaataca ttatgggaag
     1021 gggaggaaat tgaaacatgg gatcgtaatg accgacctcg aaacgatggc ttcataacag
     1081 tacctcgtta tcttccatta ctaggtgttc ttatggatga gttatcaaag ggaagcccac
     1141 tttcaagtac ctacctagcg ctgtggttca ggggttcaga cgaaggcctg atagagataa
     1201 gggataagac tgtgctggct ttagaatcag gctttgccag tgcaaggggg gtaacaacat
     1261 ggacggggag aatgcggaaa ttaaaagagc tgggatttat atcatgccgg gaaggtagct
     1321 ccggtgagtt ccacaacgtg ttgattgttc atccgctggt tgcggtaaag aaactactgg
     1381 atgaagggaa aataacgaaa gggaaaacct ataacacttt tgctgaacgg gtaatcgagg
     1441 tgaagtcctc atgggagtaa gaaatggcag gtacggtatg gaatgatgtt aatacagggc
     1501 gtgaagtttt ctcgatgaca cacctgcacc ctcagctgca taaggttgag gtttctgggg
     1561 tactggttga gattgagatt agcttcgggt ttcatgtatt tactgaccaa aaacaggtcg
     1621 gaaaggtaat gagtttcaga ggggaaacta gacatttctg ccaagaaaga tacgaaggaa
     1681 gtaagactat cgtgcagagg atactgtcag cgatagaaaa tggagagtac ataacggcct
     1741 ttatttccaa gggacaaggg cagcgatatt accaccttaa ccatcatgat gacttcattc
     1801 taatggagat aagaaagcct caaggtaaag ataattgtct tcgaatacac gttgttacag
     1861 cgtatacgat ggatgaatgg ggggtggtta acaaaggacg caatttgaag ttcaggtatg
     1921 ttctggagca gaggttgcag gggaagaaaa tcgtataaaa acaaaaagcg ccctaggcgc
     1981 ctttgtttta ctgcaaaaat ttcggtctgg cctttagtta tcctgctcac tggctaggag
     2041 ggttttcccc cggcatcagc acccctcttt caaggttccg gtttgtgcta acaggtatat
     2101 tgtctactta cccatgcctc ttgtcaactt cacgggttta tcatgatctt cttgagatcc
     2161 tttgtctatc gtcgtagtct cttgtcctgt aaacgaaaaa accgccctgc aaggcggttt
     2221 ttcgaaggtt ctcagagcta ccaactcttt gaaccgcggt aactggcttg gaggagcgca
     2281 gtaaccaaat ctgtcctttc agtttagcct taacaggcgc ataacttcaa gactaactcc
     2341 tctaaatccg ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt ccgggttgga
     2401 ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg gttcgtgcat
     2461 acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagata
     2521 aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca
     2581 cgagggagcc accaggggga aacgcctggt atctttaagt cctgtcgggt ttcgccacca
     2641 ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg
     2701 cttcgccgtg gccttatcgc ttctccgtta agcttcttcc tggcatctcc caggaaattt
     2761 ccgccccgcc tgtaagttgg taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2821 gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgctc cgctgctgca
     2881 tttttctcct gccacatgaa gcactttcct gaaccctcac cccgtcgtaa catactaagc
     2941 cagtatacac tccgctagcg ctgcgtgact ggttcagggc tgcgccccga aacccgctaa
     3001 cacccctcag ctactggata tcacaacatg ccatctgagt atgatgaaca acgggatgcg
     3061 actgatgctc tgcttcccgg cgaatatgac ctttttgatt taatggcgca ggttactgaa
     3121 gagaacctgc acgccccggt ggattttggg ccacctgtcg gccgtgaagc gttatagctt
     3181 tttcgcttac ccgcgtacgt gtaatacgcc ggtattacat cttatttctt tattttcatg
     3241 tgattacagg tggttgtaat acgttgtgta ttacataaat aaacgggaac ttcagttttt
     3301 accttttaat tcatgcaatt gcgatgaaaa tcgcggttgc gtatggtgta tgaccatttt
     3361 aaggttg
//
