LOCUS       NZ_CP092638             2155 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Enterobacter cloacae strain EFN743 plasmid p3, complete sequence.
ACCESSION   NZ_CP092638
VERSION     NZ_CP092638.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26208495
            Assembly: GCF_023920605.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Saito,R. and Mahazu,S.
  TITLE     OXA 181 producing Enterobacter cloacae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Saito,R. and Mahazu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) Department of Molecular Microbiology, Tokyo
            Medical And Dental University, 1 Chome-5-45 Yushima, Tokyo 1138510,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 159.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 03:33:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,237
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 28 of 85
            Pseudo Genes (incomplete)         :: 60 of 85
            Pseudo Genes (internal stop)      :: 11 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            Pseudo Genes (short protein)      :: 1 of 85
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092638.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="EFN743"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /plasmid="p3"
                     /geo_loc_name="Ghana"
                     /lat_lon="4.9239 N 1.7446 W"
                     /collection_date="2019"
                     /collected_by="ENRH_Hospital staff"
     gene            join(2143..2155,1..101)
                     /locus_tag="MKZ88_RS26140"
                     /old_locus_tag="MKZ88_26140"
     CDS             join(2143..2155,1..101)
                     /locus_tag="MKZ88_RS26140"
                     /old_locus_tag="MKZ88_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072202106.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            409..711
                     /locus_tag="MKZ88_RS26145"
                     /old_locus_tag="MKZ88_26145"
     CDS             409..711
                     /locus_tag="MKZ88_RS26145"
                     /old_locus_tag="MKZ88_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_023207139.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGVHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            966..1154
                     /locus_tag="MKZ88_RS26150"
                     /old_locus_tag="MKZ88_26150"
     CDS             966..1154
                     /locus_tag="MKZ88_RS26150"
                     /old_locus_tag="MKZ88_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1202..1429)
                     /locus_tag="MKZ88_RS26155"
                     /old_locus_tag="MKZ88_26155"
     CDS             complement(1202..1429)
                     /locus_tag="MKZ88_RS26155"
                     /old_locus_tag="MKZ88_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224019247.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     Typing          complement(1609..1781)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="100.0"
                     /evalue="2.04e-62"
     Typing          2..101
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.079"
                     /coverage="100.0"
                     /evalue="1.08e-31"
     Typing          1888..2155
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.026"
                     /coverage="87.0"
                     /evalue="8.620000000000001e-125"
ORIGIN
        1 ccgttcagtc cgactgctgc gccttttccg gtaactatca acttgagtcc aacccggaaa
       61 gacacgacaa agcgccactg gcagcagcca ctggtaactg atttagagga gttagtcttg
      121 aagttatgcg cctgttaagg ctaaactgaa agaacagatt tggttcctgc gctcctcgta
      181 agccagttac cacggttcaa agagttgctg ctctgagaac cttcgaaaaa ccgccttgct
      241 gggcggtttt ttcgtttaca gagcaagaga ttacgacgct acagaaagga tctcaagaag
      301 atccttcacc ataatggaaa tctatcccac ccgagcatat aggatgcata tcgttataaa
      361 acatcggata ttatgcatcc caactaaaaa taagctcgag gataaaaaat gagttcaatg
      421 gtttactgcc gaggatgcgg caaggaaata cacgaaacag caaagtcatg cccacactgc
      481 ggagccacaa acgcctcttc aggttcaggt gaaaaaagta gaatcgcagc cgcgctatta
      541 gcattcttcc ttggtggatt tggcgtacat aaattttact taggtaaaat aggacaggga
      601 ttcctgtatc taattttttg ctggacattc atacccgcga tcatcgcgtt tattgagttt
      661 attatttatc tatgcgattc cgatgaaaaa ttcgcaagaa aatatggcta actcatagca
      721 aaaaagccct gcggggcttt ttttgtacca gtctgtgagg cagatttacc ataacatccg
      781 ttgcccgcac cgcctgagac gccctcagcg cgtttctgag gactccggcc tgaaacatac
      841 acctttctgt gcccatatcg agaaaaaacg cccctgatga tttctgcgta cctgcatggc
      901 tatgcactca agtgaatgaa gtaagatttc agtctgcgat caccataaaa ccgggaaaaa
      961 gccgtatgac aaaacaggaa tcagccgcac tgaacatggc aaaatttatc cgggcgcagt
     1021 cgctgctgct gctcgaaaaa ctcgacgtgc tcgatcttga tgacgaggca gccgactgtg
     1081 agcgaatgca cgaacaggca gaagcgcttt atcagaagct gagcgcacgc ttcggtatac
     1141 aggacggcga ataaagagac gaaaagaacg ctcacttaga cgaaaagcgt atccagggca
     1201 actatgtata tccaccgtgt acatagtcat gcctgacgct ttcgagcagg tcgagcatgt
     1261 aggaacgacg agcttgattg gtatcggaca caatatggcg atacgcctca aacataacag
     1321 cacccgcatc accgcccgaa cggcgaagca tagcggaaat catccgcaca aagagcagac
     1381 gttcatcggg gcaaaggttc ataagttctc ctggtgaaaa accaaacatg aaagcaccca
     1441 tctattaacg tcagctgtga acgcttcatt agaagaaaat aggtaaaccc gggaaaaaga
     1501 tccaaacgtt aacatcacag gtaaacagaa acgtcgggtc gatcgggaaa ttctttcccg
     1561 gacggcgcgg ggttgggcaa gccgcaggcg cgtcagtgct tttagcgggt gtcggggcgc
     1621 agccctgaac cagtcacgta gcgctagcgg agtgtatact ggcttactat gtaggcacgg
     1681 gtgcgggaat cagtgaagtg cttcatgtgg caggaggaaa atgcagcagc agagcgtaag
     1741 cggaatatat gagacaggag atattccgct tcctcgctca ctgactcgct gcgctcggtc
     1801 gttcggctgc ggcgagcggt gcgggcttac gaatggggcg gaaatctcct gggagatgcc
     1861 aggaagaaac ttaacaggga aacgataagg ccgcggcgaa gccgtttttc cataggctcc
     1921 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggcggcga aacccgacag
     1981 gactataaag atcccaggcg tttccccctg gtagctccct cgtgcgctct cctgttcctg
     2041 cctttcggtt taccggtgtc attccgctgt tatggccgcg tttatctcat tccacgcctg
     2101 acactcagtt ccgggaaggc agttcgctcc aagctggact gtatgcacga acccc
//
