LOCUS       NZ_CP092637             9294 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Enterobacter cloacae strain EFN743 plasmid p2, complete sequence.
ACCESSION   NZ_CP092637
VERSION     NZ_CP092637.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26208495
            Assembly: GCF_023920605.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Saito,R. and Mahazu,S.
  TITLE     OXA 181 producing Enterobacter cloacae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Saito,R. and Mahazu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) Department of Molecular Microbiology, Tokyo
            Medical And Dental University, 1 Chome-5-45 Yushima, Tokyo 1138510,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 159.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 03:33:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,237
            CDSs (total)                      :: 5,123
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 28 of 85
            Pseudo Genes (incomplete)         :: 60 of 85
            Pseudo Genes (internal stop)      :: 11 of 85
            Pseudo Genes (multiple problems)  :: 12 of 85
            Pseudo Genes (short protein)      :: 1 of 85
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092637.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="EFN743"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /plasmid="p2"
                     /geo_loc_name="Ghana"
                     /lat_lon="4.9239 N 1.7446 W"
                     /collection_date="2019"
                     /collected_by="ENRH_Hospital staff"
     gene            join(9162..9294,1..167)
                     /locus_tag="MKZ88_RS26085"
                     /old_locus_tag="MKZ88_26085"
     CDS             join(9162..9294,1..167)
                     /locus_tag="MKZ88_RS26085"
                     /old_locus_tag="MKZ88_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(229..606)
                     /locus_tag="MKZ88_RS26090"
                     /old_locus_tag="MKZ88_26090"
     CDS             complement(229..606)
                     /locus_tag="MKZ88_RS26090"
                     /old_locus_tag="MKZ88_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            675..854
                     /locus_tag="MKZ88_RS26095"
                     /old_locus_tag="MKZ88_26095"
     CDS             675..854
                     /locus_tag="MKZ88_RS26095"
                     /old_locus_tag="MKZ88_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(882..1412)
                     /locus_tag="MKZ88_RS26100"
                     /old_locus_tag="MKZ88_26100"
     CDS             complement(882..1412)
                     /locus_tag="MKZ88_RS26100"
                     /old_locus_tag="MKZ88_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1419..2150)
                     /locus_tag="MKZ88_RS26105"
                     /old_locus_tag="MKZ88_26105"
     CDS             complement(1419..2150)
                     /locus_tag="MKZ88_RS26105"
                     /old_locus_tag="MKZ88_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(2150..4114)
                     /locus_tag="MKZ88_RS26110"
                     /old_locus_tag="MKZ88_26110"
     CDS             complement(2150..4114)
                     /locus_tag="MKZ88_RS26110"
                     /old_locus_tag="MKZ88_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5595..5744)
                     /locus_tag="MKZ88_RS26115"
                     /old_locus_tag="MKZ88_26115"
     CDS             complement(5595..5744)
                     /locus_tag="MKZ88_RS26115"
                     /old_locus_tag="MKZ88_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5829..6086)
                     /locus_tag="MKZ88_RS26120"
                     /old_locus_tag="MKZ88_26120"
     CDS             complement(5829..6086)
                     /locus_tag="MKZ88_RS26120"
                     /old_locus_tag="MKZ88_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(6096..7781)
                     /locus_tag="MKZ88_RS26125"
                     /old_locus_tag="MKZ88_26125"
     CDS             complement(6096..7781)
                     /locus_tag="MKZ88_RS26125"
                     /old_locus_tag="MKZ88_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8184..8603
                     /locus_tag="MKZ88_RS26130"
                     /old_locus_tag="MKZ88_26130"
     CDS             8184..8603
                     /locus_tag="MKZ88_RS26130"
                     /old_locus_tag="MKZ88_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            8909..9172
                     /locus_tag="MKZ88_RS26135"
                     /old_locus_tag="MKZ88_26135"
     CDS             8909..9172
                     /locus_tag="MKZ88_RS26135"
                     /old_locus_tag="MKZ88_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     BGC             1096..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4914..5492
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1423..2150)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tggccgactt cccgcgactc tatgaagtgg actcacgcta tggcgaggac gttcgccgga
       61 tcagtctgat gggacagcat gtcctctatg atgttgatga ccagaccaga aaagtccggg
      121 ttctggccgt ggtcggccag cgccagaatc cccatattgt tctctaataa ccggtgacgg
      181 ggacgcaatt cattgcgtgg ccgtcgccgg ttggacaagc acagcgcgtc agcgttttcc
      241 ccacggccac caccggcgcg gtgttccggc cggggtctct gctgtctccg gtttgtgttc
      301 cagcagtctc atggactgca tttcctgccg tagcccggcc acctcctccc tgagtgcttt
      361 tatctccgcg atcaacgcgt cggaggccat tttttcagtc tcacccgaca gtctcaactc
      421 gtcccgctcg tgagtctcat cctgtcttaa ctcaccgtac acccggataa gctcgctggt
      481 atccaccacc cggccgccat cagcttccgt acggtatgat acgcggcctt tcgccatatc
      541 ccgatacagg gaacttcggg ctttacccgt cagttccgtc gcctgtctca ccgtcaccca
      601 cgccattgtc tcaccccgct atttttgaga cagtatacct gacaggcgca caccatgccc
      661 ggaaggaaag aatcatgaca aaacaggaaa aaacagccct caatatggct aaatttatta
      721 aggagcagtc gctgctattg ctcgataaac tgaatgagct ggatatggat accccggccg
      781 acatgtgcga gcggctacac gaggatgcgg aaacactctg gcaggtgatg cgggataagc
      841 tcggggagga ataacagcac cggcatggcc ggtgctggca tttatttttt gccgaacggc
      901 caccaccggc gggcaggttt gtcctccggc agttcaggta ccggcacgtt aaaatagcgg
      961 ctgttatatt cccgcatcag ctccgctttc agcttctcgt tcaccgcgct ttccccgtgc
     1021 cgctcactca ccacgtcaat catcgcgtta atctgctccg gtgagcactt cccggcatca
     1081 accacctgca caccgttctc gatggcgaac cgctccacct tgcgatagag gtaatcacac
     1141 agcttcattc ctgtgggttt gttccgttcc cattgcggat ttttaccagt gaaaaagtac
     1201 gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc cgatggattt catcatcgcc
     1261 agatgctggg cccggtcgac aaaacagaaa acatacgccc cggtctgcca ggcgagcacc
     1321 accatatctt ccagatgctc gctggtcggc atgatgcagc gctcgccgtt ccggtcctcc
     1381 gcgtcttcaa taaataccat gtacagcgcc ataatccctc actccgttgt cataaattta
     1441 aagcacgcaa acaaccgttc acgatccagc tctgccgttt tgcgggcctc gccggtctgt
     1501 gggttcacga tttcatccac cgacaggatt gcccgctgca ccctgacctg cgtcgcccgg
     1561 tccgggcaga tgtaccagat ttcatcgatg ccgttagctt tgcggttgaa cagatacccg
     1621 gacaccactt cctgataccg gcgacggctc ttgacggtgc gctccacctc aaacgccacc
     1681 gttttaccgt ccagctccgc cagggcgtcc ggcactttca tgcctttaag actcagacgg
     1741 tgcagaatgc gccagccggt ggcccctctg gcttccatat caaggcgcag ggattgcacg
     1801 gccagccggt gcggcagatg tgctgccgac acccttgacg gctgaaacgg aataacatcc
     1861 gccagcggtt cgtcctcgtc gaacgagaac gccacgccat gcggcgtcag cccccacacg
     1921 ctgacctgac ggccgtccac cggatatttg gtccggacca gcagcccttt cgccaccagg
     1981 ctgtcgagga agcggtagac cggctgcacc gccgcaatcc ccagccgccg cgcaaccagc
     2041 tccgccgtgg aacacagctc ttcccgcaac cagcgcaggg tcagccgggt tttctccgcg
     2101 ttccgcgcct gtcgtttcgc gtggctgacg ttgtacagct ccagcgccat cagtaaatcc
     2161 ccgcaaaatc atcatccgct ttcggcccgc cgccgtcggc cgggggccgg aactccgcca
     2221 ggggatcggt tttcgccagc accgggaaac gctgcaacgg gatttcccgg cgctcaaccc
     2281 gcagcggact gctgaacgcc atccgggcca cacccagccc ggtcatgacc gcgaacccgt
     2341 ccggcaggtg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc tggatgaagc
     2401 tgtccccgct gatatgctcc acgttccccg cctcccggct ggcacggcgg cgctccacgt
     2461 ccaccctgat ttccccggtc tgccccgccg cccactgcgc ggactccagg ctggccgccc
     2521 ggtaaaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaat tcagcgggca
     2581 gatcctgccc gcactgcccg aaatcgccca gcgactggtg cgcgagcaac agattgcagt
     2641 cccggctgcg cagcgtcccc agtgcgttca gtacatactt cgacagcaga tatttaattt
     2701 catcgagcat cacgctgacg tgtggccact cccggaactc gtcccgcgca atgatgattt
     2761 gcgcgcagcg ggcaaacagc attttttgca cacggatcac cgcctcgtcg tccatcgagc
     2821 cgataacgta caggcagccg ccgccgttga taatccccgc cacatcgatg ccactgtccg
     2881 tctgcaggac gctcaggtca gcgacgtttt ccagcctagt gatcaacccc ttcacgtcct
     2941 ttttcagggc ctccggcagc gcgtacgccg ccgccagcac ctgctgaatg tttgccccct
     3001 gcggaaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg tagtggtccg
     3061 ccacgtcgcc ggtctcgccc aggttaagcc cggcaatcag caggtttttc agcgcgtact
     3121 ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga tccagcaggg
     3181 tgaagtccgg acagtgcgcc cggaagacgt gcggcgccca tgcgtcccct ttggggtcga
     3241 agtacaccac cgcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg gtggccatca
     3301 cgctcttgcc gctcccgggc aggccgagga tctgaatgtt ggtcttgcgc caggtatcac
     3361 gcggaaccag caccggcttg ccctgctcat ccttacccag aaacaccgcc tgctcctgcc
     3421 gggcctgctc cgaggaaaaa tcggccagcg cattaaccgg taccggctcc ccgaaatgca
     3481 ggtggcgcac gtcggtcatc tctcctgtcc gctggctgct gcgccggctc tcctgattca
     3541 gtttcgcgtt caccgcctcg tcagcagccg caaaatgacc gtcaagcagc aggaacgcca
     3601 gcagccgcgc caacacaatc ccgacagcca gcccgcccgc gcaccaggca atcccgaaca
     3661 ggttagccgg ctgcagcagg taataatctg cgtttgcgta gaggtccgga cgcaggacgg
     3721 tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg gccagaccgg
     3781 ccgcgccggt cagccagtac gcgtagttcc agacatgtcg acgtgccctg accatccgcc
     3841 cgggcgaatc cagcgaggcc agcaccgaaa atggcgctgg ccgtttatta tcgcgcttat
     3901 agcgatcggc atactcccgt cgctccttat gactcagccc cacaaaacgc atccgtccgg
     3961 cccaaagttc agccagaccg aaaaacaggc cgataactag gaaagccccg gccagaccgt
     4021 aatactcctg gtacagccag ctgacccacg gataaatctg ccgggccagc tccggaatgt
     4081 gccacttcca ggcaagcgaa tccgtattaa acatgttctc tctccttcag acgatggttt
     4141 gccggcgtga tgtgcaaccc ccgctgcaca agccgcggac atgccccaag gcatggcggc
     4201 ggcggtcacg tccgtggtga ttgcgttcag ctttcaccac ggaggccagc ctctttccgc
     4261 cccatgcgga aaaggcgcag ccccggagga acaacgagca cgccccagcg tgtgagagga
     4321 aaaggcggca ccccagtgcg gtcttttccg gtgttccggc ccccgtcaga gttcgccata
     4381 gcgagcggct gaccacccgc aaaatcagga gcagctttgc tgcttcgcca ggtgcgtcca
     4441 gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac gggggccggg tgaatttctg
     4501 gcgtggccgg cgtccagccg gacggagcca gaaagagggg gagagaccgt gcgtcggtgg
     4561 cgtccagcca gcgtaagcag gggatatccc cctgaggtca gccacgccct cgcggggatg
     4621 acctgtattt ctccgtaagg agaaaaaagc aacagagata ggttaacgta aaatgttcag
     4681 cgaaaaaccg atatgattca cctatcctgg taatttatta tcagggaata taaagactta
     4741 atcaggggac aatatgccac atcagcgcgt ccggtgtccg tcttgtaggt gccggggaaa
     4801 ttgcgcctcc tcgctcactc ggtcgctacg ctccggccgt gagactgcgg cgggcgctgc
     4861 ggacacatgc aaagttaccc acagattccg tgaataagca ggggactaac atgtgaggca
     4921 aaacggcaag gccacgcagg tggcgttttt ccataggctc cgccctcctg actgagttca
     4981 cataaacaga cgctttttcg gtgcatctgt gggagccggt ggctcaacca tgaatcagtc
     5041 aggagtggcg aaacccgaca ggacttaaag atcatcaccg tttccgatga atcgctccct
     5101 cttgcgctct cctgttccaa ccctgccgtt tcccggatac ctgttccgcc tttctccctt
     5161 gcgggaagtg tggcgctttc tcatagctca cactctggta tctcggctcg gtgtaggtcg
     5221 ttcgctccaa gctgggctgt aagcaagaac cccccgttca gcccgactgc tgcgccttat
     5281 ccggtaactg ttcacttgag tccaacccgg aaaagcacgc aaaacgccac tggcagcagc
     5341 cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt gctcttgaag
     5401 tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc
     5461 cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcccca
     5521 ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat ctcaagaaga
     5581 tcctttgatc ttttctactg aaccgcgatc cccgtcagtt cagaggagga cgatggtgcc
     5641 accgttccac cctgaacatc ccggatatag tttgcctgac atgcgaccag aagaaagcct
     5701 gaaaaaatca atataaaaag aaaaacaacc tttacctttt tcatatacct tccctgtatt
     5761 taaaaacccc ggtattcatc cctgaacacc ggaagtctgt atttcaaatc atccttgata
     5821 acttttgctt accagttacc atcccggtaa acaaaggata caaagtaatc aaatttacta
     5881 atatcgatta catttttaaa gtgtggctgt aatatcgaaa cccatgattt ttcaacatca
     5941 aaccaaccat tattaatatt atcctttaaa ggcatcccca gactttcaat gacagaacca
     6001 tcatcaccga agtcttttga gtattcacca cctttaaact cttcggtttt cttatcaaac
     6061 caatgaatat gtaatttaag ccccatattt acctcttaaa gatatttttt aatgtttcgt
     6121 tttggatccg gccctttaac ctgcttaccc gtttttggat cgaatgcccc gaggtgttcg
     6181 ccatcactgg cgcggtaccc ttcaagctca ccgtgctggg aatcccattc ataaatttta
     6241 cgctttttat caccatacca tcgggcccgc ttaccaccac caccttgttt aggggtttta
     6301 ggtttaccct ctttcaactc tcccaacccc ttaatatctt cagtcttggg atcaggaaaa
     6361 taatcatggc cgtaatcttt agttccctta cgaggttttt tcttttcctc atttaattta
     6421 ttttctgcat ccttcgcttt tttctccttt tgtttccggc tctccatcgc cccggaaagc
     6481 gcagccttat ccctggataa ggattcattc atttcgttta aagatgtctg ggcagctttt
     6541 aatttctcat tctgattctg aatatcctta tcaagatagc gaacctgatc ctgccacccg
     6601 gagccatatt catgaggcgg gttataagtc acaaaatcat ctttatttgc ctctgcctct
     6661 tttacctttt tatcggcttc ctgaacagca gcccttgcac cggggatcgc cttctctgtc
     6721 gatgcaatgc gactgtttaa ggtcgcaata tttttatctt cagcatccag ctcagctttc
     6781 gctttatcat actctctttt cagcccctct tccgggtgag cagcgtccca tgcctgctgg
     6841 cgacgctttt cttcctccag acgctgtttt acctgtgccg gagtaagaac atccgtcacc
     6901 gacacataga ccggtttctg tccgctctct ttcgggaaac gaataacggc ttcacgggag
     6961 ttaccaccgg ccgtaaaacc agccggacgt gaatcacctt tttcagcaat aatgcctttt
     7021 ggcggggctt tcgcggccgg aacaccttta ggtacgctca cctgcagaga cgggagaccc
     7081 ggaatcgaca cactgaatac ccccggacgt tttgtcggtt tcgcatccac cacaggaaca
     7141 ctcattggcc ggccggtgac aaccgcaata tgctgccgct cgtccttcac aatatccgcc
     7201 acacgtttag tgacgctgac cgtcgcctgg tccagcggca gggagctgac cggcgtctcc
     7261 gttaccgtgt ctgccggcaa tgacgtcacg atttttgaca tcattttcgg atcatctttt
     7321 gctatctcag aaggcagcac tccgtaaatc gcaatccccc acagaccaaa cttaaacgga
     7381 cctttcaggg cagccagcac atcagcaaca gcggccgaca acgcatcgcc ggaaacggat
     7441 aaagccagtc cttcagcccc cggagtggcc aaggcaggca gaccgaaggc catcgcggtg
     7501 gccgaagact gtccgccacc agagctcccg ctatgattgc cgctgttacc gccattacca
     7561 ccgggtttac tcccgccgtt accaaactcc cccgggttat agttatgaat atccccgtta
     7621 ggtgtatgcg tcgtacccca gcccggcccg ctgtttggat ccgtaccacc acctgatgaa
     7681 gggccacctt tacctgatgt accgttcaca ttcccactgg cctgaccacc attgtgtccc
     7741 agacctgaat tacccggacc tcgtccgtct ccgccactca tatacttttc ctctttgact
     7801 tttaaaacaa taaactaaaa taaaatactg tacatataac cactggtttt atatacagta
     7861 taaaaattac gctgccttgt ttttcatgtc aagtctgtaa atttgcgttt ttgtgaaaat
     7921 gatcaaaaaa acagccactc acaccatgcg caatcccctc cagatgcaac aaaaaccagc
     7981 acaaacggcg ccgttctccg gcctcagtat gttttactga aatcattata tgagatacca
     8041 gaaagccccc tccgggacag gaaaagaaac ggggggtctg gttcgggttg ttcggtcact
     8101 gaacacggtc agaaactgat gataagatag cctgcggata tataactcaa tgagaaaaaa
     8161 gaattaaagg taaggagacg ctcatgagac gttccggcag caaactgtgt cttatcgcca
     8221 tgataacagc gacagcaact ctcactgcct gtgctccaaa gggcagcgtg gaacagcata
     8281 cccggcatta cgtatatgct tcagatgacg gttttgatcc caatttttcc acccagaaag
     8341 ctgacacaac acgaatgatg gtgccttttt tccggcagtt ctgggatatg ggcgcaaagg
     8401 ataaagcgac cggaaaatca cggagtgatg tgcagcaacg cattcagcag tttcacagtc
     8461 aggaattttt aaactcactc caaggcacaa cacaatttgc gggtaccgat taccgcagca
     8521 aagacctcac accgaaaaaa tcaaggctac tggctgacac catttcagct gtttatctcg
     8581 atggctatga aggcagacag taagtggatt taccataacg cgtcattacg cgcaccgcga
     8641 aaacggcctc agggcgtttc cggcggctac gggataaaac aggcaacaac ccccccggaa
     8701 aactgccgga aacgcgcccg ataaatttta tcctaatgca tgcctatgca cgcaactgca
     8761 tggcatatgt tttgacgtaa aatggatgcc gtaagccctt ttttaccgac tttctgcttc
     8821 atcagcctga ctgacacgac catattcttt cagaccgccc cctgctaaac ttccttcata
     8881 ttgccgcatt aacccgggag gctccgcaat gagcaacact acacactacg aaaacgccaa
     8941 ctttcttcgg gaactggctg aaagcctgcc ccgcatactg ccggaaggtg gcccggacaa
     9001 agctgcgctg ttgcagcgac tggctaacga agaactggcc caggcagagt atgaagatca
     9061 ggttcgcgcc aaagtgaccg ccgcccgtgc tgacacgcgt ccgggtatga ccacggaaca
     9121 actccgccag cgcctgcatg gccgttatca ggaactgcgc gatgccgtat gacgtggaat
     9181 ggaagcaggg agccatcgag gatgtaacga cgctgtttga ctatatcgcg gaaaattcat
     9241 ccctgtggga tgcgcagaac gttaccgacc gggttctcgc tgcggcggac aaac
//
