LOCUS       NZ_CP092595             9294 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Klebsiella pneumoniae strain EFN 299 plasmid p6, complete sequence.
ACCESSION   NZ_CP092595
VERSION     NZ_CP092595.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26139404
            Assembly: GCF_023920325.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Saito,R. and Mahazu,S.
  TITLE     OXA 181 producing Klebsiella spp.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Saito,R. and Mahazu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2022) Department of Molecular Microbiology, Tokyo
            Medical And Dental University, 1 Chome-5-45 Yushima, Tokyo 1138510,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler hybrid assembly v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 42.7x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 03:40:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,709
            CDSs (total)                      :: 5,585
            Genes (coding)                    :: 5,454
            CDSs (with protein)               :: 5,454
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 52 of 131
            Pseudo Genes (incomplete)         :: 73 of 131
            Pseudo Genes (internal stop)      :: 21 of 131
            Pseudo Genes (multiple problems)  :: 15 of 131
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092595.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="EFN 299"
                     /isolation_source="sputum culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p6"
                     /geo_loc_name="Ghana"
                     /lat_lon="4.9239 N 1.7446 W"
                     /collection_date="2018"
                     /collected_by="ENRH_Hospital staff"
     gene            complement(1065..1214)
                     /locus_tag="MKT41_RS28430"
                     /old_locus_tag="MKT41_28420"
     CDS             complement(1065..1214)
                     /locus_tag="MKT41_RS28430"
                     /old_locus_tag="MKT41_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1299..1556)
                     /locus_tag="MKT41_RS28435"
                     /old_locus_tag="MKT41_28425"
     CDS             complement(1299..1556)
                     /locus_tag="MKT41_RS28435"
                     /old_locus_tag="MKT41_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(1566..3251)
                     /locus_tag="MKT41_RS28440"
                     /old_locus_tag="MKT41_28430"
     CDS             complement(1566..3251)
                     /locus_tag="MKT41_RS28440"
                     /old_locus_tag="MKT41_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3654..4073
                     /locus_tag="MKT41_RS28445"
                     /old_locus_tag="MKT41_28435"
     CDS             3654..4073
                     /locus_tag="MKT41_RS28445"
                     /old_locus_tag="MKT41_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            4379..4642
                     /locus_tag="MKT41_RS28450"
                     /old_locus_tag="MKT41_28440"
     CDS             4379..4642
                     /locus_tag="MKT41_RS28450"
                     /old_locus_tag="MKT41_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            4632..4931
                     /locus_tag="MKT41_RS28455"
                     /old_locus_tag="MKT41_28445"
     CDS             4632..4931
                     /locus_tag="MKT41_RS28455"
                     /old_locus_tag="MKT41_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4993..5370)
                     /locus_tag="MKT41_RS28460"
                     /old_locus_tag="MKT41_28450"
     CDS             complement(4993..5370)
                     /locus_tag="MKT41_RS28460"
                     /old_locus_tag="MKT41_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            5439..5618
                     /locus_tag="MKT41_RS28465"
                     /old_locus_tag="MKT41_28455"
     CDS             5439..5618
                     /locus_tag="MKT41_RS28465"
                     /old_locus_tag="MKT41_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5646..6176)
                     /locus_tag="MKT41_RS28470"
                     /old_locus_tag="MKT41_28460"
     CDS             complement(5646..6176)
                     /locus_tag="MKT41_RS28470"
                     /old_locus_tag="MKT41_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6183..6914)
                     /locus_tag="MKT41_RS28475"
                     /old_locus_tag="MKT41_28465"
     CDS             complement(6183..6914)
                     /locus_tag="MKT41_RS28475"
                     /old_locus_tag="MKT41_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(6914..8878)
                     /locus_tag="MKT41_RS28480"
                     /old_locus_tag="MKT41_28470"
     CDS             complement(6914..8878)
                     /locus_tag="MKT41_RS28480"
                     /old_locus_tag="MKT41_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..8251
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          384..962
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6187..6914)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gaaagagggg gagagaccgt gcgtcggtgg cgtccagcca gcgtaagcag gggatatccc
       61 cctgaggtca gccacgccct cgcggggatg acctgtattt ctccgtaagg agaaaaaagc
      121 aacagagata ggttaacgta aaatgttcag cgaaaaaccg atatgattca cctatcctgg
      181 taatttatta tcagggaata taaagactta atcaggggac aatatgccac atcagcgcgt
      241 ccggtgtccg tcttgtaggt gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg
      301 ctccggccgt gagactgcgg cgggcgctgc ggacacatgc aaagttaccc acagattccg
      361 tgaataagca ggggactaac atgtgaggca aaacggcaag gccacgcagg tggcgttttt
      421 ccataggctc cgccctcctg actgagttca cataaacaga cgctttttcg gtgcatctgt
      481 gggagccggt ggctcaacca tgaatcagtc aggagtggcg aaacccgaca ggacttaaag
      541 atcatcaccg tttccgatga atcgctccct cttgcgctct cctgttccaa ccctgccgtt
      601 tcccggatac ctgttccgcc tttctccctt gcgggaagtg tggcgctttc tcatagctca
      661 cactctggta tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac
      721 cccccgttca gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg
      781 aaaagcacgc aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt
      841 gtttagctaa acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg
      901 acagatttgg ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag ttccccaact
      961 gacttaacct tcgatcaaac cacctcccca ggtggttttt tcgtttacag ggcaaaagat
     1021 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgcgatc
     1081 cccgtcagtt cagaggagga cgatggtgcc accgttccac cctgaacatc ccggatatag
     1141 tttgcctgac atgcgaccag aagaaagcct gaaaaaatca atataaaaag aaaaacaacc
     1201 tttacctttt tcatatacct tccctgtatt taaaaacccc ggtattcatc cctgaacacc
     1261 ggaagtctgt atttcaaatc atccttgata acttttgctt accagttacc atcccggtaa
     1321 acaaaggata caaagtaatc aaatttacta atatcgatta catttttaaa gtgtggctgt
     1381 aatatcgaaa cccatgattt ttcaacatca aaccaaccat tattaatatt atcctttaaa
     1441 ggcatcccca gactttcaat gacagaacca tcatcaccga agtcttttga gtattcacca
     1501 cctttaaact cttcggtttt cttatcaaac caatgaatat gtaatttaag ccccatattt
     1561 acctcttaaa gatatttttt aatgtttcgt tttggatccg gccctttaac ctgcttaccc
     1621 gtttttggat cgaatgcccc gaggtgttcg ccatcactgg cgcggtaccc ttcaagctca
     1681 ccgtgctggg aatcccattc ataaatttta cgctttttat caccatacca tcgggcccgc
     1741 ttaccaccac caccttgttt aggggtttta ggtttaccct ctttcaactc tcccaacccc
     1801 ttaatatctt cagtcttggg atcaggaaaa taatcatggc cgtaatcttt agttccctta
     1861 cgaggttttt tcttttcctc atttaattta ttttctgcat ccttcgcttt tttctccttt
     1921 tgtttccggc tctccatcgc cccggaaagc gcagccttat ccctggataa ggattcattc
     1981 atttcgttta aagatgtctg ggcagctttt aatttctcat tctgattctg aatatcctta
     2041 tcaagatagc gaacctgatc ctgccacccg gagccatatt catgaggcgg gttataagtc
     2101 acaaaatcat ctttatttgc ctctgcctct tttacctttt tatcggcttc ctgaacagca
     2161 gcccttgcac cggggatcgc cttctctgtc gatgcaatgc gactgtttaa ggtcgcaata
     2221 tttttatctt cagcatccag ctcagctttc gctttatcat actctctttt cagcccctct
     2281 tccgggtgag cagcgtccca tgcctgctgg cgacgctttt cttcctccag acgctgtttt
     2341 acctgtgccg gagtaagaac atccgtcacc gacacataga ccggtttctg tccgctctct
     2401 ttcgggaaac gaataacggc ttcacgggag ttaccaccgg ccgtaaaacc agccggacgt
     2461 gaatcacctt tttcagcaat aatgcctttt ggcggggctt tcgcggccgg aacaccttta
     2521 ggtacgctca cctgcagaga cgggagaccc ggaatcgaca cactgaatac ccccggacgt
     2581 tttgtcggtt tcgcatccac cacaggaaca ctcattggcc ggccggtgac aaccgcaata
     2641 tgctgccgct cgtccttcac aatatccgcc acacgtttag tgacgctgac cgtcgcctgg
     2701 tccagcggca gggagctgac cggcgtctcc gttaccgtgt ctgccggcaa tgacgtcacg
     2761 atttttgaca tcattttcgg atcatctttt gctatctcag aaggcagcac tccgtaaatc
     2821 gcaatccccc acagaccaaa cttaaacgga cctttcaggg cagccagcac atcagcaaca
     2881 gcggccgaca acgcatcgcc ggaaacggat aaagccagtc cttcagcccc cggagtggcc
     2941 aaggcaggca gaccgaaggc catcgcggtg gccgaagact gtccgccacc agagctcccg
     3001 ctatgattgc cgctgttacc gccattacca ccgggtttac tcccgccgtt accaaactcc
     3061 cccgggttat agttatgaat atccccgtta ggtgtatgcg tcgtacccca gcccggcccg
     3121 ctgtttggat ccgtaccacc acctgatgaa gggccacctt tacctgatgt accgttcaca
     3181 ttcccactgg cctgaccacc attgtgtccc agacctgaat tacccggacc tcgtccgtct
     3241 ccgccactca tatacttttc ctctttgact tttaaaacaa taaactaaaa taaaatactg
     3301 tacatataac cactggtttt atatacagta taaaaattac gctgccttgt ttttcatgtc
     3361 aagtctgtaa atttgcgttt ttgtgaaaat gatcaaaaaa acagccactc acaccatgcg
     3421 caatcccctc cagatgcaac aaaaaccagc acaaacggcg ccgttctccg gcctcagtat
     3481 gttttactga aatcattata tgagatacca gaaagccccc tccgggacag gaaaagaaac
     3541 ggggggtctg gttcgggttg ttcggtcact gaacacggtc agaaactgat gataagatag
     3601 cctgcggata tataactcaa tgagaaaaaa gaattaaagg taaggagacg ctcatgagac
     3661 gttccggcag caaactgtgt cttatcgcca tgataacagc gacagcaact ctcactgcct
     3721 gtgctccaaa gggcagcgtg gaacagcata cccggcatta cgtatatgct tcagatgacg
     3781 gttttgatcc caatttttcc acccagaaag ctgacacaac acgaatgatg gtgccttttt
     3841 tccggcagtt ctgggatatg ggcgcaaagg ataaagcgac cggaaaatca cggagtgatg
     3901 tgcagcaacg cattcagcag tttcacagtc aggaattttt aaactcactc caaggcacaa
     3961 cacaatttgc gggtaccgat taccgcagca aagacctcac accgaaaaaa tcaaggctac
     4021 tggctgacac catttcagct gtttatctcg atggctatga aggcagacag taagtggatt
     4081 taccataacg cgtcattacg cgcaccgcga aaacggcctc agggcgtttc cggcggctac
     4141 gggataaaac aggcaacaac ccccccggaa aactgccgga aacgcgcccg ataaatttta
     4201 tcctaatgca tgcctatgca cgcaactgca tggcatatgt tttgacgtaa aatggatgcc
     4261 gtaagccctt ttttaccgac tttctgcttc atcagcctga ctgacacgac catattcttt
     4321 cagaccgccc cctgctaaac ttccttcata ttgccgcatt aacccgggag gctccgcaat
     4381 gagcaacact acacactacg aaaacgccaa ctttcttcgg gaactggctg aaagcctgcc
     4441 ccgcatactg ccggaaggtg gcccggacaa agctgcgctg ttgcagcgac tggctaacga
     4501 agaactggcc caggcagagt atgaagatca ggttcgcgcc aaagtgaccg ccgcccgtgc
     4561 tgacacgcgt ccgggtatga ccacggaaca actccgccag cgcctgcatg gccgttatca
     4621 ggaactgcgc gatgccgtat gacgtggaat ggaagcaggg agccatcgag gatgtaacga
     4681 cgctgtttga ctatatcgcg gaaaattcat ccctgtggga tgcgcagaac gttaccgacc
     4741 gggttctcgc tgcggcggac aaactggccg acttcccgcg actctatgaa gtggactcac
     4801 gctatggcga ggacgttcgc cggatcagtc tgatgggaca gcatgtcctc tatgatgttg
     4861 atgaccagac cagaaaagtc cgggttctgg ccgtggtcgg ccagcgccag aatccccata
     4921 ttgttctcta ataaccggtg acggggacgc aattcattgc gtggccgtcg ccggttggac
     4981 aagcacagcg cgtcagcgtt ttccccacgg ccaccaccgg cgcggtgttc cggccggggt
     5041 ctctgctgtc tccggtttgt gttccagcag tctcatggac tgcatttcct gccgtagccc
     5101 ggccacctcc tccctgagtg cttttatctc cgcgatcaac gcgtcggagg ccattttttc
     5161 agtctcaccc gacagtctca actcgtcccg ctcgtgagtc tcatcctgtc ttaactcacc
     5221 gtacacccgg ataagctcgc tggtatccac cacccggccg ccatcagctt ccgtacggta
     5281 tgatacgcgg cctttcgcca tatcccgata cagggaactt cgggctttac ccgtcagttc
     5341 cgtcgcctgt ctcaccgtca cccacgccat tgtctcaccc cgctattttt gagacagtat
     5401 acctgacagg cgcacaccat gcccggaagg aaagaatcat gacaaaacag gaaaaaacag
     5461 ccctcaatat ggctaaattt attaaggagc agtcgctgct attgctcgat aaactgaatg
     5521 agctggatat ggataccccg gccgacatgt gcgagcggct acacgaggat gcggaaacac
     5581 tctggcaggt gatgcgggat aagctcgggg aggaataaca gcaccggcat ggccggtgct
     5641 ggcatttatt ttttgccgaa cggccaccac cggcgggcag gtttgtcctc cggcagttca
     5701 ggtaccggca cgttaaaata gcggctgtta tattcccgca tcagctccgc tttcagcttc
     5761 tcgttcaccg cgctttcccc gtgccgctca ctcaccacgt caatcatcgc gttaatctgc
     5821 tccggtgagc acttcccggc atcaaccacc tgcacaccgt tctcgatggc gaaccgctcc
     5881 accttgcgat agaggtaatc acacagcttc attcctgtgg gtttgttccg ttcccattgc
     5941 ggatttttac cagtgaaaaa gtacgtggct ttttcgtcgc tgttgagccg ctcgtcatac
     6001 atcccgatgg atttcatcat cgccagatgc tgggcccggt cgacaaaaca gaaaacatac
     6061 gccccggtct gccaggcgag caccaccata tcttccagat gctcgctggt cggcatgatg
     6121 cagcgctcgc cgttccggtc ctccgcgtct tcaataaata ccatgtacag cgccataatc
     6181 cctcactccg ttgtcataaa tttaaagcac gcaaacaacc gttcacgatc cagctctgcc
     6241 gttttgcggg cctcgccggt ctgtgggttc acgatttcat ccaccgacag gattgcccgc
     6301 tgcaccctga cctgcgtcgc ccggtccggg cagatgtacc agatttcatc gatgccgtta
     6361 gctttgcggt tgaacagata cccggacacc acttcctgat accggcgacg gctcttgacg
     6421 gtgcgctcca cctcaaacgc caccgtttta ccgtccagct ccgccagggc gtccggcact
     6481 ttcatgcctt taagactcag acggtgcaga atgcgccagc cggtggcccc tctggcttcc
     6541 atatcaaggc gcagggattg cacggccagc cggtgcggca gatgtgctgc cgacaccctt
     6601 gacggctgaa acggaataac atccgccagc ggttcgtcct cgtcgaacga gaacgccacg
     6661 ccatgcggcg tcagccccca cacgctgacc tgacggccgt ccaccggata tttggtccgg
     6721 accagcagcc ctttcgccac caggctgtcg aggaagcggt agaccggctg caccgccgca
     6781 atccccagcc gccgcgcaac cagctccgcc gtggaacaca gctcttcccg caaccagcgc
     6841 agggtcagcc gggttttctc cgcgttccgc gcctgtcgtt tcgcgtggct gacgttgtac
     6901 agctccagcg ccatcagtaa atccccgcaa aatcatcatc cgctttcggc ccgccgccgt
     6961 cggccggggg ccggaactcc gccaggggat cggttttcgc cagcaccggg aaacgctgca
     7021 acgggatttc ccggcgctca acccgcagcg gactgctgaa cgccatccgg gccacaccca
     7081 gcccggtcat gaccgcgaac ccgtccggca ggtgttgcag ggtgttcacg tcaaacagcg
     7141 gccgcgcctc cttctggatg aagctgtccc cgctgatatg ctccacgttc cccgcctccc
     7201 ggctggcacg gcggcgctcc acgtccaccc tgatttcccc ggtctgcccc gccgcccact
     7261 gcgcggactc caggctggcc gcccggtaaa accagcggat cggcgtgtta tccagtaccg
     7321 tggttttcac aaattcagcg ggcagatcct gcccgcactg cccgaaatcg cccagcgact
     7381 ggtgcgcgag caacagattg cagtcccggc tgcgcagcgt ccccagtgcg ttcagtacat
     7441 acttcgacag cagatattta atttcatcga gcatcacgct gacgtgtggc cactcccgga
     7501 actcgtcccg cgcaatgatg atttgcgcgc agcgggcaaa cagcattttt tgcacacgga
     7561 tcaccgcctc gtcgtccatc gagccgataa cgtacaggca gccgccgccg ttgataatcc
     7621 ccgccacatc gatgccactg tccgtctgca ggacgctcag gtcagcgacg ttttccagcc
     7681 tagtgatcaa ccccttcacg tcctttttca gggcctccgg cagcgcgtac gccgccgcca
     7741 gcacctgctg aatgtttgcc ccctgcggaa actgctccgc aatcagcttc gccgctttct
     7801 gctcgctgat gcggtagtgg tccgccacgt cgccggtctc gcccaggtta agcccggcaa
     7861 tcagcaggtt tttcagcgcg tactggtcga gatcgcggaa cagattcagc tgcgccggtt
     7921 tccccggccg cagatccagc agggtgaagt ccggacagtg cgcccggaag acgtgcggcg
     7981 cccatgcgtc ccctttgggg tcgaagtaca ccaccgcgtc cccgaaatcg cgtacgcagc
     8041 gaatgagcgc gttggtggcc atcacgctct tgccgctccc gggcaggccg aggatctgaa
     8101 tgttggtctt gcgccaggta tcacgcggaa ccagcaccgg cttgccctgc tcatccttac
     8161 ccagaaacac cgcctgctcc tgccgggcct gctccgagga aaaatcggcc agcgcattaa
     8221 ccggtaccgg ctccccgaaa tgcaggtggc gcacgtcggt catctctcct gtccgctggc
     8281 tgctgcgccg gctctcctga ttcagtttcg cgttcaccgc ctcgtcagca gccgcaaaat
     8341 gaccgtcaag cagcaggaac gccagcagcc gcgccaacac aatcccgaca gccagcccgc
     8401 ccgcgcacca ggcaatcccg aacaggttag ccggctgcag caggtaataa tctgcgtttg
     8461 cgtagaggtc cggacgcagg acggtttctc cccagctctc gataaacacg gcgacaacca
     8521 gcccccccag aagggccaga ccggccgcgc cggtcagcca gtacgcgtag ttccagacat
     8581 gtcgacgtgc cctgaccatc cgcccgggcg aatccagcga ggccagcacc gaaaatggcg
     8641 ctggccgttt attatcgcgc ttatagcgat cggcatactc ccgtcgctcc ttatgactca
     8701 gccccacaaa acgcatccgt ccggcccaaa gttcagccag accgaaaaac aggccgataa
     8761 ctaggaaagc cccggccaga ccgtaatact cctggtacag ccagctgacc cacggataaa
     8821 tctgccgggc cagctccgga atgtgccact tccaggcaag cgaatccgta ttaaacatgt
     8881 tctctctcct tcagacgatg gtttgccggc gtgatgtgca acccccgctg cacaagccgc
     8941 ggacatgccc caaggcatgg cggcggcggt cacgtccgtg gtgattgcgt tcagctttca
     9001 ccacggaggc cagcctcttt ccgccccatg cggaaaaggc gcagccccgg aggaacaacg
     9061 agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt tccggtgttc
     9121 cggcccccgt cagagttcgc catagcgagc ggctgaccac ccgcaaaatc aggagcagct
     9181 ttgctgcttc gccaggtgcg tccagcaccg tggatttttg tgggtggtca ggcgtgagcc
     9241 tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca gccggacgga gcca
//
