LOCUS       NZ_CP092580             9329 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterococcus faecalis strain VRE-WC031 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP092580
VERSION     NZ_CP092580.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26026624
            Assembly: GCF_022691485.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Haas,W., Mitchell,K.K. and Musser,K.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2022) Bacteriology, NYS DOH, 120 New Scotland Ave,
            Albany, NY 12008, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 153x
            Sequencing Technology :: Illumina MiSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022691485.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:50:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,271
            CDSs (total)                      :: 3,188
            Genes (coding)                    :: 3,052
            CDSs (with protein)               :: 3,052
            Genes (RNA)                       :: 83
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 85 of 136
            Pseudo Genes (incomplete)         :: 42 of 136
            Pseudo Genes (internal stop)      :: 26 of 136
            Pseudo Genes (multiple problems)  :: 16 of 136
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092580.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="VRE-WC031"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2004-04-02"
                     /collected_by="NYS DOH"
     gene            1..969
                     /locus_tag="MKI49_RS16215"
                     /old_locus_tag="MKI49_003221"
     CDS             1..969
                     /locus_tag="MKI49_RS16215"
                     /old_locus_tag="MKI49_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358693.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_010773543.1"
                     /translation="MANELVKYHSELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKELSNYKATANMRFIDDLEETYKKILELRFGRRSQSGLTREFFVMFTKFKINGD
                     TDEPYVDIQIFEDALPLLNDLDSWVRYSLQQFNELQSSYSKTMFRLLKQFRTKGYAYFS
                     KEDFQELLDIPKSYKQGDIDRTVLQPIKQELTAIFKGLSIKKKYGRGRGKPVIGYQFTF
                     KPEIKNANDFNKHAPTKIEQSQALSQVNGQKNYEEQLTTLDDKIKEQERRLDLLKQLKE
                     LEQNKKDEKSLQIQTENKDRFTLSKRDKKDILSGLQNFNKL"
                     /name="replication initiation protein"
     gene            complement(1948..2127)
                     /locus_tag="MKI49_RS16220"
                     /old_locus_tag="MKI49_003222"
     CDS             complement(1948..2127)
                     /locus_tag="MKI49_RS16220"
                     /old_locus_tag="MKI49_003222"
                     /inference="COORDINATES: protein motif:HMM:NF023063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin G-beta family bacteriocin"
                     /protein_id="WP_010773550.1"
                     /translation="MKNIKNASNIKVIEDNELKAITGGGPGKWLPWLQPAYDFVTGLAK
                     GIGKEGNKNKWKNV"
                     /name="lactococcin G-beta family bacteriocin"
     gene            complement(2160..2333)
                     /locus_tag="MKI49_RS16225"
                     /old_locus_tag="MKI49_003223"
     CDS             complement(2160..2333)
                     /locus_tag="MKI49_RS16225"
                     /old_locus_tag="MKI49_003223"
                     /inference="COORDINATES: protein motif:HMM:NF019739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin 1071A family bacteriocin"
                     /protein_id="WP_010773549.1"
                     /translation="MKQYKVLNEKEMKKTIGGESVFSKIGNAVGPAAYWILKGLGNMSD
                     VNQADRINRKKH"
                     /name="enterocin 1071A family bacteriocin"
     gene            2558..4651
                     /locus_tag="MKI49_RS16230"
                     /old_locus_tag="MKI49_003224"
     CDS             2558..4651
                     /locus_tag="MKI49_RS16230"
                     /old_locus_tag="MKI49_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292468.1"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_011117543.1"
                     /translation="MILKHYNTEIPIHKLRELSGTDLEGTSAFGLKRTLEKIGFDSPAI
                     QADNEVWKEKELPLPLIAHVLIDNKYMHYVVVYGIKGDTLLIADPDPTVGKVKKSMEEF
                     SKEWTGVLLLPTPKESYTPTKEKVAGLSSFLPIIWRQKGLVFHIILASLFITLFGIGSS
                     YYFQGILDYFIPNQARSTLNIVSIGLIAVYLFRVLFEYSRSYLLVILGQRMSMAVMLQY
                     FKHVLTLPMNFFATRKSGEIISRFLDANKIIDALASATLSVFLDIGMVFLVGITLAIQN
                     GTLFLITLASLPFYFVAILAFVKSYEKANQEEMAAGATLNSSIIESLKGIETIKAYSGE
                     EKVYNRVDREFIRLMKKSFRTVTLDNVQQGIKHAIQLISSALILWLGSFYVMDGTISLG
                     QLITYNALLVFFTDPLQNIINLQVKMQTAQVANKRLNEIFAIEPEQTNDSSEREIPNQT
                     FQQEIRLENVSFSYNMKAPTLNNISCTIAPKSKIALVGVSGSGKSTLAKLLVNFYAPSE
                     GTIRYGKMNHLDIPFQQLREHVTYVPQESFFFSGTIVENLTFGLQNMPSFERIIEVCEA
                     VQLNTFIEQQPLRYDMVLEEGGANLSGGQRQRLAIARALLKDADVLILDEATSGLDTLL
                     EHAILDYLLTLEEKTLLFIAHHLPIAKACDHILVLHEGQLVEQGAHDDLRYNQGVYQQL
                     WDI"
                     /name="peptide cleavage/export ABC transporter"
     gene            4670..6037
                     /locus_tag="MKI49_RS16235"
                     /old_locus_tag="MKI49_003225"
     CDS             4670..6037
                     /locus_tag="MKI49_RS16235"
                     /old_locus_tag="MKI49_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_011117544.1"
                     /translation="MQQKDWLDHSSVYSQQHHTFYRWVLYPVILFLCLGALFLAFAKKE
                     VVIRTPAQLTAQKTEKLQVPVEAKIKTNTLKENQTVKKGEELVTFETTPLQNEKKQLEQ
                     ENTTIEEQKKAAQTFIDSLTNEQNLFETEDSFGYSNQVKSFLAEKEATRYASKQSETNT
                     QKEQEAYNKTKEQLEKQLTTRQKEQKEWEQVRTAWINEQSLEGFSTEITSRYQAWQSQL
                     TDVPEEQKKQIKATTLATIDEQISQVKKEIEQLQSEQSKLVAPTTSDNEVSSQTEKTKQ
                     TKELALATAKQKVVELTETQKKNETALKTINEQLAQSTLKAPITGTVHVNEEVKNQLEV
                     PKGTTLAEIYPKSKDPQLTFTALIPTSESMRIKPGMAIHFKLDKKGVATKTIDGTLTEI
                     SENSTTTEQGAFYTIKGTLKPPKEFTGRYGLTGELSLIVGKKTYWQQIKDMLLNQE"
                     /name="bacteriocin secretion accessory protein"
     gene            6058..6366
                     /locus_tag="MKI49_RS16240"
                     /old_locus_tag="MKI49_003226"
     CDS             6058..6366
                     /locus_tag="MKI49_RS16240"
                     /old_locus_tag="MKI49_003226"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010773555.1"
                     /translation="MNELEKLQIQEQKLREKKKQLLAKEYSRLGRAFYKKAKVKSFDEA
                     QQAVTNYQPGTLGITNDQVEKLKQFANNLTWNESGNYWYTNEVKELTQFLAQFREEV"
                     /name="hypothetical protein"
     gene            6970..7353
                     /locus_tag="MKI49_RS16245"
                     /old_locus_tag="MKI49_003227"
     CDS             6970..7353
                     /locus_tag="MKI49_RS16245"
                     /old_locus_tag="MKI49_003227"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_011117545.1"
                     /translation="MTEEKEQGVNRKSPKKRHYTQVNFRLTEAEKERWQKQASSLNMTL
                     PKFSKHLINLFFDSGTIKQPKIDKQQGTEIARHLAKLGGNVNQIAKWCNTHQHDISDVQ
                     AKRLAENLAHVQKELKQVWQQLN"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7335..8375
                     /locus_tag="MKI49_RS16250"
                     /old_locus_tag="MKI49_003228"
     CDS             7335..8375
                     /locus_tag="MKI49_RS16250"
                     /old_locus_tag="MKI49_003228"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010773542.1"
                     /translation="MATIKLARTTSCNRAISYAEKRAVEKDGVNCEITNAKQEMATIRQ
                     LFSKTDKTQAHLCIQSFSPQESQQLGPKKINQLGIELAEKMAPNHQVAVYTHADTDHIH
                     NHLVINAVNLETGNKYSHHNDFQRVSALHDQLLQEHQLQIVEKQAVERRTMAERKLAEK
                     GQWVWKDQIRQTIDALMKDSSISSYKAFREGLDQRGIKLHERGQNVVYELLEGNKRVRG
                     AKLGTDYEKDVIKHELDHREQTRTIETQARTLANGLRTSDFSRGSDEITHDPRRDSPID
                     FRMGTAISDLKHQLRQATETPSERTERKNRERVEALKQTDTRPIRHTHQPEPDLTPRQS
                     GPSLGL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8461..8775
                     /locus_tag="MKI49_RS16255"
                     /old_locus_tag="MKI49_003229"
     CDS             8461..8775
                     /locus_tag="MKI49_RS16255"
                     /old_locus_tag="MKI49_003229"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_115357941.1"
                     /translation="MKELTANLEKAQEEVQEAMGMFARKQLAQAKTVNNIKDVAMSAVF
                     VLITLAVLWTVGYLLGTFFQLDEFYRWLVASTIGKGVLWGLGLALLVGSIGSVVYSVKN
                     "
                     /name="hypothetical protein"
     BGC             1..9329
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..988
                     /name="rep_cluster_1070"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.595"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7336..8372
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaacg aacttgttaa gtatcattca gaattaaata ccattccttt gaggaaattt
       61 actcctgtag aaatgaactt gttcttctct attgtttcta gaatgcgtga aaaaggcgac
      121 aagacagttc gttttacttt tgaccaatta aaagaattaa gcaattataa agcgactgct
      181 aatatgcgtt tcattgatga tttagaagaa acatataaaa aaattttaga gttgcgattc
      241 ggcaggagaa gtcaaagtgg cttaactaga gaattttttg tgatgtttac taaattcaaa
      301 attaacggtg atactgacga accttatgta gatattcaaa ttttcgaaga tgcacttcct
      361 ctgttaaatg acttagattc ttgggttcgc tactcattac aacaattcaa tgaactacaa
      421 agtagttatt ctaaaaccat gtttcgttta ttgaaacaat ttcgtaccaa aggatatgcg
      481 tatttctcta aagaagactt tcaagaattg ttagatattc caaaaagcta taaacaagga
      541 gatattgata gaactgtttt acagcctatt aaacaagaac ttactgctat cttcaaaggt
      601 ttatcaatta aaaagaaata tggaagaggt cgcggtaaac ccgttattgg ttatcaattc
      661 acgtttaaac ccgagatcaa aaatgctaat gatttcaata aacatgcccc tactaaaatc
      721 gaacagagtc aggcgttatc tcaggtaaac gggcaaaaaa attatgaaga acaactaaca
      781 actttggacg ataagattaa agaacaagag cgtagattag acttattaaa acaactcaaa
      841 gaattagagc aaaataaaaa agacgagaaa agcttgcaaa tacagaccga gaataaagat
      901 agatttactc tttcaaaaag ggataaaaaa gatatactta gcggtttaca aaatttcaat
      961 aaattatagt aagaagcaca tagtttagca gcaatataaa tatattaaac caactatatc
     1021 aaatatctat tgaatacctt aaataaattt attttcaata ctttactttt ggagggattg
     1081 ctatgttatt tttactaaaa tttattacgc tattaagcat tatttcttat ttatatctat
     1141 gtattatgta tatgaaaaat atggacaaat ataaattatt agcattaata cttatagggg
     1201 gctgcatttt attatctatc gttctaagta tatttaactt atcgtttatc ttaattagac
     1261 tgtctctatt acttggatta tctggccttt tactattgct taaaccagcc attaaaaata
     1321 catttaaaga tgatttttca tttttagaag gtatagcatt aagtgctata acatcaagtt
     1381 acttagtaag cttaatttca ttatttatac ggtcttaatt attattgtgc gtttttctaa
     1441 aaggtaatac aaatataata aacctgtggt gctagttgat ttttcttaaa atcaactagc
     1501 accacaggtt tattattacg ttaaaggaaa gagcccataa tatattaatt tacttaatta
     1561 aatagttcag tacaatgtat atacattgag taaaaaagaa taacacaaac attgtttgat
     1621 ttttttgtta aaactgattt cttttttaaa attatatata aatatataat agtaattatg
     1681 ctcaacaatt ctaacaaatt agttaacaga cttaaattga aaggtactat catagtaaaa
     1741 accattagaa tgaatacgct aaagaaaaat gtataagctt tttttattgc atgatctgaa
     1801 taattgatat caattccaat taaaatgtat aaaaatgtta taaaattaaa aaaggttatg
     1861 atgcttataa aaatatccat atttatctcc ttaaaaataa taaaaacata tggcgttaac
     1921 gccatatgtt tttattatac tctttaatta tacattcttc cacttatttt tatttccttc
     1981 ttttccaatt ccttttgcaa gacctgtaac aaaatcatat gcgggttgta gccatggtaa
     2041 ccattttcca ggtcctccac cagtaattgc tttcaactca ttatcttcaa ttacttttat
     2101 attacttgca ttcttaatgt ttttcatatt agcatatctc ctctatttga tttcgattct
     2161 taatgtttct ttctattaat tctatcagct tggtttacat cactcatatt acctaatcct
     2221 tttaaaatcc aataagcagc tggacctaca gcattaccta ttttactaaa aaccgactct
     2281 cccccaatag tttttttcat ttctttttca ttcaatactt tatattgctt catttatcgt
     2341 tccccctaat tatttttgta atataattct ggaattatat tacttaattt attaaatcaa
     2401 agccttttta ttatgtcaat taattatata aaaaggtttt caaaaatatt ttattatagt
     2461 aatattgata aatgaataat aacacaagtg aggagaaact ttgaaaaaga ttatttacca
     2521 acaagacgaa aaagattgtg gcgtagcttg tattgccatg attttaaagc attataatac
     2581 agaaataccc attcacaagt tacgtgagtt gtctggaaca gatttagagg gaacttccgc
     2641 ttttggtcta aaaagaactt tagaaaaaat tggttttgat tctcctgcta ttcaagctga
     2701 taatgaagta tggaaagaaa aagaattacc gttaccttta attgcgcatg tcttaattga
     2761 caataaatac atgcactatg tcgtggttta tggaataaaa ggagatacct tattaattgc
     2821 tgatccagat ccaacagtag gaaaagtaaa aaaatcaatg gaagaatttt ccaaagaatg
     2881 gacaggcgtt ttactgttac ctacacctaa agagtcttat actcctacca aagaaaaagt
     2941 cgctggattg agctcttttt tacccattat ttggcgtcaa aaaggcttag tttttcacat
     3001 tattttagcg tctcttttta ttacgctatt tgggattggg agctcctact attttcaagg
     3061 aattttagat tactttattc ctaatcaagc acgttctact ttaaacattg tttcgattgg
     3121 cttgattgct gtctatctgt tccgtgtcct gtttgaatac agtcggagtt atctcttagt
     3181 tatcctagga caacgcatga gcatggccgt aatgcttcaa tactttaaac atgttcttac
     3241 tttacctatg aacttttttg ctacccgtaa atcaggggag atcatttctc gttttctaga
     3301 cgcaaataaa atcattgatg ccctcgccag tgcaacctta tccgtctttt tagacattgg
     3361 gatggtcttc ttagtaggga ttaccttagc cattcaaaat ggtacattat tcttaattac
     3421 cttagcttcg ttaccgtttt attttgtggc cattctagcc tttgtcaaaa gttatgaaaa
     3481 agccaatcaa gaagaaatgg cggctggcgc taccttaaat tcaagtatta tcgagagcct
     3541 aaaaggaatc gagacgatca aggcctacag tggagaagaa aaagtctata atcgcgtgga
     3601 tcgagaattt attcgattaa tgaaaaagtc cttccggact gttacgctag ataatgtgca
     3661 acaagggatt aaacatgcta ttcagttaat cagtagtgcc cttattttgt ggctaggttc
     3721 tttctatgtc atggatggta cgattagcct tggccaattg attacttaca atgccttgtt
     3781 ggtgtttttc acggatcctt tacagaatat tattaatcta caagtcaaaa tgcaaacagc
     3841 gcaagtggcc aacaaacgct taaacgagat ttttgccatt gaaccagaac aaacaaacga
     3901 ttcttcggaa cgcgaaattc caaatcaaac gttccaacaa gaaataagac tagaaaatgt
     3961 ctccttctcc tataacatga aagcacctac cttaaacaat atttcttgca ccattgcacc
     4021 gaaaagtaaa atcgccttgg ttggggtaag tggttcaggg aaatcaacat tagcaaaatt
     4081 attagtgaac ttttatgcgc cttcagaagg aaccattcgc tacggaaaaa tgaatcattt
     4141 agatattccg tttcaacaat tacgtgaaca cgtcacctat gtgccgcaag agtctttttt
     4201 ctttagcggg acaattgtag aaaacctaac gtttggctta cagaacatgc ctagttttga
     4261 acggattatt gaagtgtgtg aagccgttca actaaacaca ttcattgaac aacagccttt
     4321 acgctatgac atggtactag aagaaggagg ggcgaatctt tcaggaggac aacggcaacg
     4381 attggcgatt gctcgtgctc ttttgaaaga cgcggatgtt ttaatcttag acgaagcaac
     4441 tagcggatta gacacactat tagaacatgc gattcttgat tatttactaa cgttagaaga
     4501 gaaaacattg ctttttattg cgcaccattt gcccattgct aaagcgtgcg atcacattct
     4561 agttcttcat gaagggcaac tggttgaaca aggggctcat gacgacctcc gttataatca
     4621 gggggtttat caacagttat gggatattta ggaaaggaca gaaaaattga tgcaacaaaa
     4681 ggattggctc gatcattcga gtgtttatag ccaacagcac cacaccttct atcgatgggt
     4741 cttgtatcct gtcattcttt ttttgtgctt aggggcctta tttttagctt ttgcgaaaaa
     4801 agaagtcgtg attcgaacac ctgcacaact aacggcacaa aagacagaaa aactacaagt
     4861 accggtagaa gcaaagataa aaacaaatac tctgaaagaa aaccaaacag taaaaaaggg
     4921 cgaagagtta gtcacttttg aaacaacccc tttgcaaaac gaaaaaaaac aattagagca
     4981 agaaaacaca acgattgagg aacaaaaaaa agcggcacaa acttttattg acagcctaac
     5041 aaatgaacaa aatttatttg agacagaaga ttccttcggc tatagcaatc aagtaaagag
     5101 ttttctggct gaaaaagagg caacgcgcta tgcctcaaag caaagtgaaa caaacacaca
     5161 aaaggagcaa gaagcgtaca acaaaaccaa agaacaacta gaaaaacaac taactacgcg
     5221 tcaaaaagaa caaaaggaat gggagcaggt acgcacggca tggatcaatg aacaatccct
     5281 agagggattt tcaactgaaa tcacatccag gtatcaggcg tggcaatctc aattaaccga
     5341 cgtaccagaa gaacaaaaga aacaaattaa agcaacgaca ttggctacaa tcgacgaaca
     5401 aatcagtcag gtaaaaaaag aaatcgagca acttcaaagc gaacaatcaa agctagtagc
     5461 gccaaccacg tctgataatg aagtaagtag tcaaacagaa aaaacaaagc aaaccaaaga
     5521 actagccttg gcaacagcga aacaaaaagt ggtagaatta accgagaccc aaaagaaaaa
     5581 tgagacagca ctgaaaacaa tcaacgaaca actcgcacaa agtacgttga aagcacctat
     5641 cacaggaact gttcacgtaa atgaggaagt caaaaaccaa ttggaagtcc cgaaaggcac
     5701 aacgttggca gaaatttatc cgaaatcaaa agacccacaa cttacgttta ccgcacttat
     5761 tcctacgagt gaaagcatga gaatcaaacc tggaatggca atacatttca aactggataa
     5821 gaaaggagta gcaactaaaa caatcgacgg aacgctaacg gaaatttcag aaaacagtac
     5881 gacaactgaa caaggagctt tttatacaat aaaaggcaca ctaaaacctc caaaagagtt
     5941 taccggtcgc tatggtctta caggggagct ttctctcatt gttggaaaga aaacctattg
     6001 gcaacaaatc aaagatatgt tattaaatca agaatagaaa aagagtgagg gattatcatg
     6061 aatgagttag aaaaactaca aatccaagaa caaaaactac gagaaaagaa aaagcaacta
     6121 ctcgcaaagg aatacagccg tttaggacgt gccttttaca aaaaagcgaa agtgaaatcc
     6181 tttgacgaag cccaacaagc cgtcaccaat tatcaaccag gaacactcgg cataacaaac
     6241 gatcaagtag aaaaattaaa acagtttgct aataacctaa cctggaatga atcaggcaac
     6301 tattggtata cgaatgaggt caaagaatta acacaatttt tggcacaatt ccgcgaggaa
     6361 gtgtaagtcg attgaacggg tttagttgct tcgataatcc gaagcaactt ttcctccatt
     6421 tatggaggaa aaaaggggcg ttttcgaaca gaaaaccgac cctgaaaacg ttggagaaaa
     6481 atcgccgtac aggcgacctt tttcaagcaa cgttcggggg attgggggat acccccaaca
     6541 agctatttgt agtacgaagt gctagcaaat aaactgcttg ccaaaacctc aggatgggca
     6601 cataatgagg ttgcctgttt tgttggtaag tggtgactga ctaaggaaca acgaacctac
     6661 aaaacttaga ggaaaggtgt aaccgaatag ggcacaactt gacgttttct cttgtgagag
     6721 ttgtgagtga caaacgaaca attggcacta gagaaaggct aaagaaaatt gaccgaatag
     6781 ggaatttttc tggcgcgatc ttttgatttt ttttcgtagc cgtaggcacg aaaacagaca
     6841 aagggaaacg actgaagaag gagttttctc tgtctgcttg gggtgagatt gtcgaacaga
     6901 caactcgcaa cccaagtgac ggaacaaatc ggcgaacagc cgactttgag aaggaaagga
     6961 gcgaacacaa tgacagaaga aaaagagcaa ggagtgaacc ggaaatcccc caaaaagcgc
     7021 cactatacgc aagtgaattt tcgtttaact gaagccgaga aagaacgctg gcaaaaacaa
     7081 gccagtagcc tgaacatgac cttaccaaag ttctcaaaac acttgattaa tttattcttt
     7141 gattctggga caatcaagca accaaaaatt gacaaacaac aaggaacgga aattgcccgc
     7201 cacttagcca aactaggcgg aaatgtgaat caaatcgcca agtggtgcaa tacccatcaa
     7261 cacgacattt cagacgttca ggctaaacgg ttagccgaga acttagcaca cgtgcaaaag
     7321 gagttgaaac aggtatggca acaattaaac tagcgcgtac caccagttgc aatcgggcca
     7381 ttagctatgc ggaaaagcgc gccgtggaaa aagacggcgt caactgcgaa atcaccaacg
     7441 ccaagcaaga aatggccacg attcgccaac tcttctcaaa aaccgataaa acacaagccc
     7501 atttgtgtat ccaatccttt agcccacaag agagccaaca acttgggcca aagaaaatca
     7561 atcagttagg catagaattg gccgagaaaa tggcccccaa ccatcaagta gcggtctata
     7621 cccacgccga cacagaccat atccataacc atctggtgat caatgcggtg aatttagaaa
     7681 ccggcaacaa atacagccac cataacgact ttcagcgcgt atctgccctc catgaccagc
     7741 tattgcagga acaccagtta cagatcgtcg aaaaacaagc ggtggaacgt cggaccatgg
     7801 ccgaacgaaa actcgccgaa aaagggcaat gggtttggaa agaccaaatc aggcagacaa
     7861 tcgacgcctt gatgaaggat agctctataa gctcatataa ggcgtttaga gagggtttgg
     7921 atcaaagagg tataaaatta catgaacgag gtcaaaacgt cgtatacgag cttttagagg
     7981 ggaataagag ggttcgcgga gcgaagctag gaacagatta tgaaaaggac gtgattaaac
     8041 atgagttgga ccaccgagaa caaacacgaa caattgaaac acaagcaaga acgcttgcaa
     8101 atggacttag aacgagcgat tttagtcgag gaagtgacga aattacgcac gatccaagaa
     8161 gagattcccc aattgatttc agaatgggaa cagcaatttc agaccttaag caccaacttc
     8221 gacaagcaac agaaacacca tcagaacgaa ctgagcgaaa aaatcgagaa cgagttgaag
     8281 cacttaaaca gacggataca agaccaatta gacacactca tcagccagaa ccagatctta
     8341 caccacgaca atcaggtcct tctctcggac tatgaaaact acctggacaa acatgtcaaa
     8401 gaaatcaatc aagaataccg cctacttgtc gaacaccaag cccttgcaca ggtgagccat
     8461 ttgaaagaac tgacagcaaa cctggaaaaa gcacaagaag aagtgcaaga agcgatgggt
     8521 atgttcgcta gaaaacagtt agcccaggca aaaacagtga ataacatcaa agacgtggcc
     8581 atgagtgccg tttttgtctt gattacactc gctgttcttt ggacagtggg ctatctatta
     8641 ggcacgtttt tccagttgga cgaattttat cggtggttag ttgcctcaac cattggcaaa
     8701 ggcgttctct gggggctagg gcttgcactc ctagttggat caattggtag tgtggtttat
     8761 tcggtaaaga actaatggtc tttccagggt cgttagggct tgccctgacc gtctgaaaga
     8821 cgcaaacaag gaaaactatc aaggaatgct taactcgtct tagaaaggct gacagtggca
     8881 gttaggacga tcccacaggt ctccttgttg tgggtttgct tttagcgagt gaccgaaagg
     8941 gaacgctgca agtacaatgt gggagcaaag cgagcactcc ttttagcgaa caacgtgagc
     9001 gaagcatttc cagtttattg attacgcgaa ccagttttta gaaaaaagag gtctttttta
     9061 ggcgggaatt tttgcccccc tcttttataa actcttttta aacctctttt aaacctcttt
     9121 accgtcggct gtactccacg ccctcaaagg agtttatcgt tttatgaacg tagttttgtc
     9181 tgtttatgaa cgtagttttg tctgtttatg aacgtagttt tgtctgttta tgaacgtagt
     9241 tttgtctgtt tagaagtttc tttataaacg tattacgttt tacaaatacg tttcaatatg
     9301 gtagtctttt cttcaagagg tgactatat
//
