LOCUS       NZ_CP092575             9331 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterococcus faecalis strain VSE-WC032 plasmid unnamed, complete
            sequence.
ACCESSION   NZ_CP092575
VERSION     NZ_CP092575.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26026625
            Assembly: GCF_022691465.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Haas,W., Mitchell,K.K. and Musser,K.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2022) Bacteriology, NYS DOH, 120 New Scotland Ave,
            Albany, NY 12008, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 158x
            Sequencing Technology :: Illumina MiSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022691465.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 05:53:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,100
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,899
            CDSs (with protein)               :: 2,899
            Genes (RNA)                       :: 82
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 76 of 119
            Pseudo Genes (incomplete)         :: 32 of 119
            Pseudo Genes (internal stop)      :: 27 of 119
            Pseudo Genes (multiple problems)  :: 15 of 119
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092575.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="VSE-WC032"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed"
                     /geo_loc_name="USA"
                     /collection_date="2004-04-02"
                     /collected_by="NYS DOH"
     gene            1..969
                     /locus_tag="MKI56_RS15380"
                     /old_locus_tag="MKI56_003056"
     CDS             1..969
                     /locus_tag="MKI56_RS15380"
                     /old_locus_tag="MKI56_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358693.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_010773543.1"
                     /translation="MANELVKYHSELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKELSNYKATANMRFIDDLEETYKKILELRFGRRSQSGLTREFFVMFTKFKINGD
                     TDEPYVDIQIFEDALPLLNDLDSWVRYSLQQFNELQSSYSKTMFRLLKQFRTKGYAYFS
                     KEDFQELLDIPKSYKQGDIDRTVLQPIKQELTAIFKGLSIKKKYGRGRGKPVIGYQFTF
                     KPEIKNANDFNKHAPTKIEQSQALSQVNGQKNYEEQLTTLDDKIKEQERRLDLLKQLKE
                     LEQNKKDEKSLQIQTENKDRFTLSKRDKKDILSGLQNFNKL"
                     /name="replication initiation protein"
     gene            complement(1950..2129)
                     /locus_tag="MKI56_RS15385"
                     /old_locus_tag="MKI56_003057"
     CDS             complement(1950..2129)
                     /locus_tag="MKI56_RS15385"
                     /old_locus_tag="MKI56_003057"
                     /inference="COORDINATES: protein motif:HMM:NF023063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin G-beta family bacteriocin"
                     /protein_id="WP_010773550.1"
                     /translation="MKNIKNASNIKVIEDNELKAITGGGPGKWLPWLQPAYDFVTGLAK
                     GIGKEGNKNKWKNV"
                     /name="lactococcin G-beta family bacteriocin"
     gene            complement(2162..2335)
                     /locus_tag="MKI56_RS15390"
                     /old_locus_tag="MKI56_003058"
     CDS             complement(2162..2335)
                     /locus_tag="MKI56_RS15390"
                     /old_locus_tag="MKI56_003058"
                     /inference="COORDINATES: protein motif:HMM:NF019739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin 1071A family bacteriocin"
                     /protein_id="WP_010773549.1"
                     /translation="MKQYKVLNEKEMKKTIGGESVFSKIGNAVGPAAYWILKGLGNMSD
                     VNQADRINRKKH"
                     /name="enterocin 1071A family bacteriocin"
     gene            2560..4653
                     /locus_tag="MKI56_RS15395"
                     /old_locus_tag="MKI56_003059"
     CDS             2560..4653
                     /locus_tag="MKI56_RS15395"
                     /old_locus_tag="MKI56_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292468.1"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_011117543.1"
                     /translation="MILKHYNTEIPIHKLRELSGTDLEGTSAFGLKRTLEKIGFDSPAI
                     QADNEVWKEKELPLPLIAHVLIDNKYMHYVVVYGIKGDTLLIADPDPTVGKVKKSMEEF
                     SKEWTGVLLLPTPKESYTPTKEKVAGLSSFLPIIWRQKGLVFHIILASLFITLFGIGSS
                     YYFQGILDYFIPNQARSTLNIVSIGLIAVYLFRVLFEYSRSYLLVILGQRMSMAVMLQY
                     FKHVLTLPMNFFATRKSGEIISRFLDANKIIDALASATLSVFLDIGMVFLVGITLAIQN
                     GTLFLITLASLPFYFVAILAFVKSYEKANQEEMAAGATLNSSIIESLKGIETIKAYSGE
                     EKVYNRVDREFIRLMKKSFRTVTLDNVQQGIKHAIQLISSALILWLGSFYVMDGTISLG
                     QLITYNALLVFFTDPLQNIINLQVKMQTAQVANKRLNEIFAIEPEQTNDSSEREIPNQT
                     FQQEIRLENVSFSYNMKAPTLNNISCTIAPKSKIALVGVSGSGKSTLAKLLVNFYAPSE
                     GTIRYGKMNHLDIPFQQLREHVTYVPQESFFFSGTIVENLTFGLQNMPSFERIIEVCEA
                     VQLNTFIEQQPLRYDMVLEEGGANLSGGQRQRLAIARALLKDADVLILDEATSGLDTLL
                     EHAILDYLLTLEEKTLLFIAHHLPIAKACDHILVLHEGQLVEQGAHDDLRYNQGVYQQL
                     WDI"
                     /name="peptide cleavage/export ABC transporter"
     gene            4672..6039
                     /locus_tag="MKI56_RS15400"
                     /old_locus_tag="MKI56_003060"
     CDS             4672..6039
                     /locus_tag="MKI56_RS15400"
                     /old_locus_tag="MKI56_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_011117544.1"
                     /translation="MQQKDWLDHSSVYSQQHHTFYRWVLYPVILFLCLGALFLAFAKKE
                     VVIRTPAQLTAQKTEKLQVPVEAKIKTNTLKENQTVKKGEELVTFETTPLQNEKKQLEQ
                     ENTTIEEQKKAAQTFIDSLTNEQNLFETEDSFGYSNQVKSFLAEKEATRYASKQSETNT
                     QKEQEAYNKTKEQLEKQLTTRQKEQKEWEQVRTAWINEQSLEGFSTEITSRYQAWQSQL
                     TDVPEEQKKQIKATTLATIDEQISQVKKEIEQLQSEQSKLVAPTTSDNEVSSQTEKTKQ
                     TKELALATAKQKVVELTETQKKNETALKTINEQLAQSTLKAPITGTVHVNEEVKNQLEV
                     PKGTTLAEIYPKSKDPQLTFTALIPTSESMRIKPGMAIHFKLDKKGVATKTIDGTLTEI
                     SENSTTTEQGAFYTIKGTLKPPKEFTGRYGLTGELSLIVGKKTYWQQIKDMLLNQE"
                     /name="bacteriocin secretion accessory protein"
     gene            6060..6368
                     /locus_tag="MKI56_RS15405"
                     /old_locus_tag="MKI56_003061"
     CDS             6060..6368
                     /locus_tag="MKI56_RS15405"
                     /old_locus_tag="MKI56_003061"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010773555.1"
                     /translation="MNELEKLQIQEQKLREKKKQLLAKEYSRLGRAFYKKAKVKSFDEA
                     QQAVTNYQPGTLGITNDQVEKLKQFANNLTWNESGNYWYTNEVKELTQFLAQFREEV"
                     /name="hypothetical protein"
     gene            6972..7355
                     /locus_tag="MKI56_RS15410"
                     /old_locus_tag="MKI56_003062"
     CDS             6972..7355
                     /locus_tag="MKI56_RS15410"
                     /old_locus_tag="MKI56_003062"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_011117545.1"
                     /translation="MTEEKEQGVNRKSPKKRHYTQVNFRLTEAEKERWQKQASSLNMTL
                     PKFSKHLINLFFDSGTIKQPKIDKQQGTEIARHLAKLGGNVNQIAKWCNTHQHDISDVQ
                     AKRLAENLAHVQKELKQVWQQLN"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7337..8377
                     /locus_tag="MKI56_RS15415"
                     /old_locus_tag="MKI56_003063"
     CDS             7337..8377
                     /locus_tag="MKI56_RS15415"
                     /old_locus_tag="MKI56_003063"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010773542.1"
                     /translation="MATIKLARTTSCNRAISYAEKRAVEKDGVNCEITNAKQEMATIRQ
                     LFSKTDKTQAHLCIQSFSPQESQQLGPKKINQLGIELAEKMAPNHQVAVYTHADTDHIH
                     NHLVINAVNLETGNKYSHHNDFQRVSALHDQLLQEHQLQIVEKQAVERRTMAERKLAEK
                     GQWVWKDQIRQTIDALMKDSSISSYKAFREGLDQRGIKLHERGQNVVYELLEGNKRVRG
                     AKLGTDYEKDVIKHELDHREQTRTIETQARTLANGLRTSDFSRGSDEITHDPRRDSPID
                     FRMGTAISDLKHQLRQATETPSERTERKNRERVEALKQTDTRPIRHTHQPEPDLTPRQS
                     GPSLGL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8463..8777
                     /locus_tag="MKI56_RS15420"
                     /old_locus_tag="MKI56_003064"
     CDS             8463..8777
                     /locus_tag="MKI56_RS15420"
                     /old_locus_tag="MKI56_003064"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_115357941.1"
                     /translation="MKELTANLEKAQEEVQEAMGMFARKQLAQAKTVNNIKDVAMSAVF
                     VLITLAVLWTVGYLLGTFFQLDEFYRWLVASTIGKGVLWGLGLALLVGSIGSVVYSVKN
                     "
                     /name="hypothetical protein"
     BGC             1..9331
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..988
                     /name="rep_cluster_1070"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.595"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7338..8374
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaacg aacttgttaa gtatcattca gaattaaata ccattccttt gaggaaattt
       61 actcctgtag aaatgaactt gttcttctct attgtttcta gaatgcgtga aaaaggcgac
      121 aagacagttc gttttacttt tgaccaatta aaagaattaa gcaattataa agcgactgct
      181 aatatgcgtt tcattgatga tttagaagaa acatataaaa aaattttaga gttgcgattc
      241 ggcaggagaa gtcaaagtgg cttaactaga gaattttttg tgatgtttac taaattcaaa
      301 attaacggtg atactgacga accttatgta gatattcaaa ttttcgaaga tgcacttcct
      361 ctgttaaatg acttagattc ttgggttcgc tactcattac aacaattcaa tgaactacaa
      421 agtagttatt ctaaaaccat gtttcgttta ttgaaacaat ttcgtaccaa aggatatgcg
      481 tatttctcta aagaagactt tcaagaattg ttagatattc caaaaagcta taaacaagga
      541 gatattgata gaactgtttt acagcctatt aaacaagaac ttactgctat cttcaaaggt
      601 ttatcaatta aaaagaaata tggaagaggt cgcggtaaac ccgttattgg ttatcaattc
      661 acgtttaaac ccgagatcaa aaatgctaat gatttcaata aacatgcccc tactaaaatc
      721 gaacagagtc aggcgttatc tcaggtaaac gggcaaaaaa attatgaaga acaactaaca
      781 actttggacg ataagattaa agaacaagag cgtagattag acttattaaa acaactcaaa
      841 gaattagagc aaaataaaaa agacgagaaa agcttgcaaa tacagaccga gaataaagat
      901 agatttactc tttcaaaaag ggataaaaaa gatatactta gcggtttaca aaatttcaat
      961 aaattatagt aagaagcaca tagtttagca gcaatataaa tatattaaac caactatatc
     1021 aaatatctat tgaatacctt aaataaattt attttcaata ctttactttt ggagggattg
     1081 ctatgttatt tttactaaaa tttattacgc tattaagcat tatttcttat ttatatctat
     1141 gtattatgta tatgaaaaat atggacaaat ataaattatt agcattaata cttatagggg
     1201 gctgcatttt attatctatc gttctaagta tatttaactt atcgtttatc ttaattagac
     1261 tgtctctatt acttggatta tctggccttt tactattgct taaaccagcc attaaaaata
     1321 catttaaaga tgatttttca tttttagaag gtatagcatt aagtgctata acatcaagtt
     1381 acttagtaag cttaatttca ttatttatac ggtcttaatt attattgtgc gtttttctaa
     1441 aaggtaatac aaatataata aacctgtggt gctagttgat ttttcttaaa atcaactagc
     1501 accacaggtt tattattacg ttaaaggaaa gagcccataa tatattaatt tacttaatta
     1561 aatagttcag tacaatgtat atacattgag taaaaaagaa taacacaaac attgtttgat
     1621 ttttttttgt taaaactgat ttctttttta aaattatata taaatatata atagtaatta
     1681 tgctcaacaa ttctaacaaa ttagttaaca gacttaaatt gaaaggtact atcatagtaa
     1741 aaaccattag aatgaatacg ctaaagaaaa atgtataagc tttttttatt gcatgatctg
     1801 aataattgat atcaattcca attaaaatgt ataaaaatgt tataaaatta aaaaaggtta
     1861 tgatgcttat aaaaatatcc atatttatct ccttaaaaat aataaaaaca tatggcgtta
     1921 acgccatatg tttttattat actctttaat tatacattct tccacttatt tttatttcct
     1981 tcttttccaa ttccttttgc aagacctgta acaaaatcat atgcgggttg tagccatggt
     2041 aaccattttc caggtcctcc accagtaatt gctttcaact cattatcttc aattactttt
     2101 atattacttg cattcttaat gtttttcata ttagcatatc tcctctattt gatttcgatt
     2161 cttaatgttt ctttctatta attctatcag cttggtttac atcactcata ttacctaatc
     2221 cttttaaaat ccaataagca gctggaccta cagcattacc tattttacta aaaaccgact
     2281 ctcccccaat agtttttttc atttcttttt cattcaatac tttatattgc ttcatttatc
     2341 gttcccccta attatttttg taatataatt ctggaattat attacttaat ttattaaatc
     2401 aaagcctttt tattatgtca attaattata taaaaaggtt ttcaaaaata ttttattata
     2461 gtaatattga taaatgaata ataacacaag tgaggagaaa ctttgaaaaa gattatttac
     2521 caacaagacg aaaaagattg tggcgtagct tgtattgcca tgattttaaa gcattataat
     2581 acagaaatac ccattcacaa gttacgtgag ttgtctggaa cagatttaga gggaacttcc
     2641 gcttttggtc taaaaagaac tttagaaaaa attggttttg attctcctgc tattcaagct
     2701 gataatgaag tatggaaaga aaaagaatta ccgttacctt taattgcgca tgtcttaatt
     2761 gacaataaat acatgcacta tgtcgtggtt tatggaataa aaggagatac cttattaatt
     2821 gctgatccag atccaacagt aggaaaagta aaaaaatcaa tggaagaatt ttccaaagaa
     2881 tggacaggcg ttttactgtt acctacacct aaagagtctt atactcctac caaagaaaaa
     2941 gtcgctggat tgagctcttt tttacccatt atttggcgtc aaaaaggctt agtttttcac
     3001 attattttag cgtctctttt tattacgcta tttgggattg ggagctccta ctattttcaa
     3061 ggaattttag attactttat tcctaatcaa gcacgttcta ctttaaacat tgtttcgatt
     3121 ggcttgattg ctgtctatct gttccgtgtc ctgtttgaat acagtcggag ttatctctta
     3181 gttatcctag gacaacgcat gagcatggcc gtaatgcttc aatactttaa acatgttctt
     3241 actttaccta tgaacttttt tgctacccgt aaatcagggg agatcatttc tcgttttcta
     3301 gacgcaaata aaatcattga tgccctcgcc agtgcaacct tatccgtctt tttagacatt
     3361 gggatggtct tcttagtagg gattacctta gccattcaaa atggtacatt attcttaatt
     3421 accttagctt cgttaccgtt ttattttgtg gccattctag cctttgtcaa aagttatgaa
     3481 aaagccaatc aagaagaaat ggcggctggc gctaccttaa attcaagtat tatcgagagc
     3541 ctaaaaggaa tcgagacgat caaggcctac agtggagaag aaaaagtcta taatcgcgtg
     3601 gatcgagaat ttattcgatt aatgaaaaag tccttccgga ctgttacgct agataatgtg
     3661 caacaaggga ttaaacatgc tattcagtta atcagtagtg cccttatttt gtggctaggt
     3721 tctttctatg tcatggatgg tacgattagc cttggccaat tgattactta caatgccttg
     3781 ttggtgtttt tcacggatcc tttacagaat attattaatc tacaagtcaa aatgcaaaca
     3841 gcgcaagtgg ccaacaaacg cttaaacgag atttttgcca ttgaaccaga acaaacaaac
     3901 gattcttcgg aacgcgaaat tccaaatcaa acgttccaac aagaaataag actagaaaat
     3961 gtctccttct cctataacat gaaagcacct accttaaaca atatttcttg caccattgca
     4021 ccgaaaagta aaatcgcctt ggttggggta agtggttcag ggaaatcaac attagcaaaa
     4081 ttattagtga acttttatgc gccttcagaa ggaaccattc gctacggaaa aatgaatcat
     4141 ttagatattc cgtttcaaca attacgtgaa cacgtcacct atgtgccgca agagtctttt
     4201 ttctttagcg ggacaattgt agaaaaccta acgtttggct tacagaacat gcctagtttt
     4261 gaacggatta ttgaagtgtg tgaagccgtt caactaaaca cattcattga acaacagcct
     4321 ttacgctatg acatggtact agaagaagga ggggcgaatc tttcaggagg acaacggcaa
     4381 cgattggcga ttgctcgtgc tcttttgaaa gacgcggatg ttttaatctt agacgaagca
     4441 actagcggat tagacacact attagaacat gcgattcttg attatttact aacgttagaa
     4501 gagaaaacat tgctttttat tgcgcaccat ttgcccattg ctaaagcgtg cgatcacatt
     4561 ctagttcttc atgaagggca actggttgaa caaggggctc atgacgacct ccgttataat
     4621 cagggggttt atcaacagtt atgggatatt taggaaagga cagaaaaatt gatgcaacaa
     4681 aaggattggc tcgatcattc gagtgtttat agccaacagc accacacctt ctatcgatgg
     4741 gtcttgtatc ctgtcattct ttttttgtgc ttaggggcct tatttttagc ttttgcgaaa
     4801 aaagaagtcg tgattcgaac acctgcacaa ctaacggcac aaaagacaga aaaactacaa
     4861 gtaccggtag aagcaaagat aaaaacaaat actctgaaag aaaaccaaac agtaaaaaag
     4921 ggcgaagagt tagtcacttt tgaaacaacc cctttgcaaa acgaaaaaaa acaattagag
     4981 caagaaaaca caacgattga ggaacaaaaa aaagcggcac aaacttttat tgacagccta
     5041 acaaatgaac aaaatttatt tgagacagaa gattccttcg gctatagcaa tcaagtaaag
     5101 agttttctgg ctgaaaaaga ggcaacgcgc tatgcctcaa agcaaagtga aacaaacaca
     5161 caaaaggagc aagaagcgta caacaaaacc aaagaacaac tagaaaaaca actaactacg
     5221 cgtcaaaaag aacaaaagga atgggagcag gtacgcacgg catggatcaa tgaacaatcc
     5281 ctagagggat tttcaactga aatcacatcc aggtatcagg cgtggcaatc tcaattaacc
     5341 gacgtaccag aagaacaaaa gaaacaaatt aaagcaacga cattggctac aatcgacgaa
     5401 caaatcagtc aggtaaaaaa agaaatcgag caacttcaaa gcgaacaatc aaagctagta
     5461 gcgccaacca cgtctgataa tgaagtaagt agtcaaacag aaaaaacaaa gcaaaccaaa
     5521 gaactagcct tggcaacagc gaaacaaaaa gtggtagaat taaccgagac ccaaaagaaa
     5581 aatgagacag cactgaaaac aatcaacgaa caactcgcac aaagtacgtt gaaagcacct
     5641 atcacaggaa ctgttcacgt aaatgaggaa gtcaaaaacc aattggaagt cccgaaaggc
     5701 acaacgttgg cagaaattta tccgaaatca aaagacccac aacttacgtt taccgcactt
     5761 attcctacga gtgaaagcat gagaatcaaa cctggaatgg caatacattt caaactggat
     5821 aagaaaggag tagcaactaa aacaatcgac ggaacgctaa cggaaatttc agaaaacagt
     5881 acgacaactg aacaaggagc tttttataca ataaaaggca cactaaaacc tccaaaagag
     5941 tttaccggtc gctatggtct tacaggggag ctttctctca ttgttggaaa gaaaacctat
     6001 tggcaacaaa tcaaagatat gttattaaat caagaataga aaaagagtga gggattatca
     6061 tgaatgagtt agaaaaacta caaatccaag aacaaaaact acgagaaaag aaaaagcaac
     6121 tactcgcaaa ggaatacagc cgtttaggac gtgcctttta caaaaaagcg aaagtgaaat
     6181 cctttgacga agcccaacaa gccgtcacca attatcaacc aggaacactc ggcataacaa
     6241 acgatcaagt agaaaaatta aaacagtttg ctaataacct aacctggaat gaatcaggca
     6301 actattggta tacgaatgag gtcaaagaat taacacaatt tttggcacaa ttccgcgagg
     6361 aagtgtaagt cgattgaacg ggtttagttg cttcgataat ccgaagcaac ttttcctcca
     6421 tttatggagg aaaaaagggg cgttttcgaa cagaaaaccg accctgaaaa cgttggagaa
     6481 aaatcgccgt acaggcgacc tttttcaagc aacgttcggg ggattggggg atacccccaa
     6541 caagctattt gtagtacgaa gtgctagcaa ataaactgct tgccaaaacc tcaggatggg
     6601 cacataatga ggttgcctgt tttgttggta agtggtgact gactaaggaa caacgaacct
     6661 acaaaactta gaggaaaggt gtaaccgaat agggcacaac ttgacgtttt ctcttgtgag
     6721 agttgtgagt gacaaacgaa caattggcac tagagaaagg ctaaagaaaa ttgaccgaat
     6781 agggaatttt tctggcgcga tcttttgatt ttttttcgta gccgtaggca cgaaaacaga
     6841 caaagggaaa cgactgaaga aggagttttc tctgtctgct tggggtgaga ttgtcgaaca
     6901 gacaactcgc aacccaagtg acggaacaaa tcggcgaaca gccgactttg agaaggaaag
     6961 gagcgaacac aatgacagaa gaaaaagagc aaggagtgaa ccggaaatcc cccaaaaagc
     7021 gccactatac gcaagtgaat tttcgtttaa ctgaagccga gaaagaacgc tggcaaaaac
     7081 aagccagtag cctgaacatg accttaccaa agttctcaaa acacttgatt aatttattct
     7141 ttgattctgg gacaatcaag caaccaaaaa ttgacaaaca acaaggaacg gaaattgccc
     7201 gccacttagc caaactaggc ggaaatgtga atcaaatcgc caagtggtgc aatacccatc
     7261 aacacgacat ttcagacgtt caggctaaac ggttagccga gaacttagca cacgtgcaaa
     7321 aggagttgaa acaggtatgg caacaattaa actagcgcgt accaccagtt gcaatcgggc
     7381 cattagctat gcggaaaagc gcgccgtgga aaaagacggc gtcaactgcg aaatcaccaa
     7441 cgccaagcaa gaaatggcca cgattcgcca actcttctca aaaaccgata aaacacaagc
     7501 ccatttgtgt atccaatcct ttagcccaca agagagccaa caacttgggc caaagaaaat
     7561 caatcagtta ggcatagaat tggccgagaa aatggccccc aaccatcaag tagcggtcta
     7621 tacccacgcc gacacagacc atatccataa ccatctggtg atcaatgcgg tgaatttaga
     7681 aaccggcaac aaatacagcc accataacga ctttcagcgc gtatctgccc tccatgacca
     7741 gctattgcag gaacaccagt tacagatcgt cgaaaaacaa gcggtggaac gtcggaccat
     7801 ggccgaacga aaactcgccg aaaaagggca atgggtttgg aaagaccaaa tcaggcagac
     7861 aatcgacgcc ttgatgaagg atagctctat aagctcatat aaggcgttta gagagggttt
     7921 ggatcaaaga ggtataaaat tacatgaacg aggtcaaaac gtcgtatacg agcttttaga
     7981 ggggaataag agggttcgcg gagcgaagct aggaacagat tatgaaaagg acgtgattaa
     8041 acatgagttg gaccaccgag aacaaacacg aacaattgaa acacaagcaa gaacgcttgc
     8101 aaatggactt agaacgagcg attttagtcg aggaagtgac gaaattacgc acgatccaag
     8161 aagagattcc ccaattgatt tcagaatggg aacagcaatt tcagacctta agcaccaact
     8221 tcgacaagca acagaaacac catcagaacg aactgagcga aaaaatcgag aacgagttga
     8281 agcacttaaa cagacggata caagaccaat tagacacact catcagccag aaccagatct
     8341 tacaccacga caatcaggtc cttctctcgg actatgaaaa ctacctggac aaacatgtca
     8401 aagaaatcaa tcaagaatac cgcctacttg tcgaacacca agcccttgca caggtgagcc
     8461 atttgaaaga actgacagca aacctggaaa aagcacaaga agaagtgcaa gaagcgatgg
     8521 gtatgttcgc tagaaaacag ttagcccagg caaaaacagt gaataacatc aaagacgtgg
     8581 ccatgagtgc cgtttttgtc ttgattacac tcgctgttct ttggacagtg ggctatctat
     8641 taggcacgtt tttccagttg gacgaatttt atcggtggtt agttgcctca accattggca
     8701 aaggcgttct ctgggggcta gggcttgcac tcctagttgg atcaattggt agtgtggttt
     8761 attcggtaaa gaactaatgg tctttccagg gtcgttaggg cttgccctga ccgtctgaaa
     8821 gacgcaaaca aggaaaacta tcaaggaatg cttaactcgt cttagaaagg ctgacagtgg
     8881 cagttaggac gatcccacag gtctccttgt tgtgggtttg cttttagcga gtgaccgaaa
     8941 gggaacgctg caagtacaat gtgggagcaa agcgagcact ccttttagcg aacaacgtga
     9001 gcgaagcatt tccagtttat tgattacgcg aaccagtttt tagaaaaaag aggtcttttt
     9061 taggcgggaa tttttgcccc cctcttttat aaactctttt taaacctctt ttaaacctct
     9121 ttaccgtcgg ctgtactcca cgccctcaaa ggagtttatc gttttatgaa cgtagttttg
     9181 tctgtttatg aacgtagttt tgtctgttta tgaacgtagt tttgtctgtt tatgaacgta
     9241 gttttgtctg tttagaagtt tctttataaa cgtattacgt tttacaaata cgtttcaata
     9301 tggtagtctt ttcttcaaga ggtgactata t
//
