LOCUS       NZ_CP092572            12429 bp    DNA     circular CON 07-APR-2024
DEFINITION  Enterococcus faecium strain VRE-WC072 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP092572
VERSION     NZ_CP092572.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26026626
            Assembly: GCF_022691445.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Haas,W., Mitchell,K.K. and Musser,K.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2022) Bacteriology, NYS DOH, 120 New Scotland Ave,
            Albany, NY 12008, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 157x
            Sequencing Technology :: Illumina MiSeq and Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022691445.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 08:52:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 2,983
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 90 of 171
            Pseudo Genes (incomplete)         :: 79 of 171
            Pseudo Genes (internal stop)      :: 31 of 171
            Pseudo Genes (multiple problems)  :: 25 of 171
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VRE-WC072"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2004-04-20"
                     /collected_by="NYS DOH"
     gene            1..933
                     /locus_tag="MKI62_RS15165"
                     /old_locus_tag="MKI62_003027"
     CDS             1..933
                     /locus_tag="MKI62_RS15165"
                     /old_locus_tag="MKI62_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="MKI62_RS15170"
                     /old_locus_tag="MKI62_003028"
     CDS             950..1420
                     /locus_tag="MKI62_RS15170"
                     /old_locus_tag="MKI62_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002313301.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQAVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1821
                     /locus_tag="MKI62_RS15175"
                     /old_locus_tag="MKI62_003029"
     CDS             1504..1821
                     /locus_tag="MKI62_RS15175"
                     /old_locus_tag="MKI62_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002302541.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRILL
                     F"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2111..3292
                     /locus_tag="MKI62_RS15180"
                     /old_locus_tag="MKI62_003030"
     CDS             2111..3292
                     /locus_tag="MKI62_RS15180"
                     /old_locus_tag="MKI62_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002313302.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNAKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            3382..3651
                     /locus_tag="MKI62_RS15185"
                     /old_locus_tag="MKI62_003031"
     CDS             3382..3651
                     /locus_tag="MKI62_RS15185"
                     /old_locus_tag="MKI62_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="WP_002313303.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYKK
                     YPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWTASSRTAKYYYYI"
                     /name="bacteriocin 32"
     gene            4456..4629
                     /locus_tag="MKI62_RS15190"
                     /old_locus_tag="MKI62_003032"
     CDS             4456..4629
                     /locus_tag="MKI62_RS15190"
                     /old_locus_tag="MKI62_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002313306.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELRLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(5221..5334)
                     /locus_tag="MKI62_RS15195"
     CDS             complement(5221..5334)
                     /locus_tag="MKI62_RS15195"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_242425900.1"
                     /translation="MARKFNVTRQTISKWENNKTTPDIETLKKFVNFIVLT"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5558..5902
                     /locus_tag="MKI62_RS15200"
                     /old_locus_tag="MKI62_003034"
     CDS             5558..5902
                     /locus_tag="MKI62_RS15200"
                     /old_locus_tag="MKI62_003034"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_240070584.1"
                     /translation="MGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEATEEGFSSSKL
                     RYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGVRAYATCNATL
                     TGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            5847..6332
                     /locus_tag="MKI62_RS15205"
     CDS             5847..6332
                     /locus_tag="MKI62_RS15205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_242425901.1"
                     /translation="MQHAMPHLLEILVGGMFIKREFKLSFYLHLKGNSKYVKKINAVLS
                     LFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYKDDMDFNSYINALYEISTQENINI
                     SQYSFTAVNQLTILATNPNANKHWKFSKKNILARNDSKVHYTNNKNSKYQLKLPNNF"
                     /name="hypothetical protein"
     gene            6873..7655
                     /locus_tag="MKI62_RS15210"
     CDS             6873..7655
                     /locus_tag="MKI62_RS15210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_242425898.1"
                     /translation="MILLLIASTKVINIHNEAENNSLKYWERTTNLYKTNITNQLNRNN
                     TVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFIGQKVYPNFEDYVSQP
                     AGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKYKEYAKMIEKNYLEDI
                     QFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKAKDNYTITDPIVRVIN
                     PEITESLFWGNILTSDGGLFFDQKHTSNQNFLIKLIRI"
                     /name="hypothetical protein"
     gene            8060..8680
                     /locus_tag="MKI62_RS15215"
                     /old_locus_tag="MKI62_003036"
     CDS             8060..8680
                     /locus_tag="MKI62_RS15215"
                     /old_locus_tag="MKI62_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            8794..8931
                     /locus_tag="MKI62_RS15220"
                     /old_locus_tag="MKI62_003037"
     CDS             8794..8931
                     /locus_tag="MKI62_RS15220"
                     /old_locus_tag="MKI62_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            9373..9753
                     /gene="mobC"
                     /locus_tag="MKI62_RS15225"
                     /old_locus_tag="MKI62_003038"
     CDS             9373..9753
                     /gene="mobC"
                     /locus_tag="MKI62_RS15225"
                     /old_locus_tag="MKI62_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002319809.1"
                     /translation="MSEQNQNLASNYSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            9735..10649
                     /locus_tag="MKI62_RS15230"
                     /old_locus_tag="MKI62_003039"
     CDS             9735..10649
                     /locus_tag="MKI62_RS15230"
                     /old_locus_tag="MKI62_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            10609..10887
                     /locus_tag="MKI62_RS15500"
     CDS             10609..10887
                     /locus_tag="MKI62_RS15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            10889..11578
                     /locus_tag="MKI62_RS15235"
                     /old_locus_tag="MKI62_003040"
     CDS             10889..11578
                     /locus_tag="MKI62_RS15235"
                     /old_locus_tag="MKI62_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_242425899.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSRIRFKRFLLLG
                     VVFTLSIVSSGLFGVLEGNKRVSRSVLLENVTQKYEVAHEKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     BGC             558..10902
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.893"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9736..10646
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaacgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca agctgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatt atggtaaaaa atcatacaaa
     1741 aacactaata ctattatttc attttgtaat ttttttaagt ttagttttca ccttaattca
     1801 gtccggattc ttttatttta gccattcaat tgccacttgg ttattaatat tttttctaag
     1861 tttactatta ataattaata cattaataat taatacttta attattatct ttaataaaca
     1921 tagaaactta tttcttcaca gcatatttcc aattgtttta ttaatttttc tatactttat
     1981 ataaaaagat agaactattt agtaagataa aaaagtagct aattgtaatt ttcgtctaaa
     2041 actcaaaact agtaaaaata actttattgt gttattttta tttataaatt atgaaaaagg
     2101 acctttcgaa atgaaaaata atagaatcta caaaaatcag ttgaattatg ggcttttatt
     2161 gccagttttt ctgctatgta tcattggatt actttcttta tatgttgctt tatatcacga
     2221 ctcttacact aattcaaaaa tcacaaaaga attaattaaa caagtattgt ggtatagtat
     2281 tgggttcact tcagcaataa ctatttattt ttttaatcct aaaattttgt ggaaattaac
     2341 tcctatattt tacggattat gcatcataat gttattactt ctcttgaaat tctataatcc
     2401 acaattagca gtcttaactg gagcaaaaaa ttggttttat attgctggtt tttcgtttca
     2461 acctactgag ttagtcaaaa taggatacac ccttatgcta gctaaaataa ccgtcaatta
     2521 tcagcaaaaa tctattacta gtaatgcgaa agatgattgg atttatttac ttaaaacttt
     2581 tattgctact cttccaatag gaatattatt attttttcag aacgatctag gaacaatgtt
     2641 agtcttcatt gttatttata tttttatttt gtttacgtct aacataaata taaaaataat
     2701 tgccccaata atcgccattg gtgttgtttt atccttaata tttttatatt tggttatatc
     2761 agatacagga agagatttct tattaaaaat aggatttcac gactatcagt ttaaaagaat
     2821 agactcatgg ttgaatccat ttttagatcc aaatggatct agctatcagt tagcaaatag
     2881 tttaattgct attggctctg gcggactttt aggtactggt tttaatgtat caaacattca
     2941 cgttcctgtt agagaatccg atatgatatt ttctgtaatt ggagaaaatt ttggctttat
     3001 aggaagttgt cttgtaatat tcctatactt ttatttaatt tatcaaatgt ataaaacatg
     3061 ttttaaaagc aataacttat ttttcaccta tatcggtata ggcattgttg caatgatttt
     3121 ttttcatgtt tttgaaaata taggtgcaag tatcggatta ctacctctaa ctggtattcc
     3181 actcccctat attagtcaag gtggttctgc cattcttagt aattttttgg ggttgggatt
     3241 tatgttgtgt tgtagtagat atactaacga aaaaaaagga acacaaaaat gaattcaaaa
     3301 taacacttta tgaatagaat gtctatttca ataaaaatta tgatacctta aagttaatca
     3361 ttaaaaaagg agtgtcataa tttgaaaaaa acaaaattat tggtagctag tttgtgtttg
     3421 tttagcagtt tattagcttt caccccttct gtttcatttt ctcaaaatgg tggagtggtt
     3481 gaagctgctg ctcaaagagg atatatctac aaaaaatatc caaaaggtgc taaagtgcca
     3541 aataaagtta aaatgttagt aaatattaga ggtaaacaaa ctatgcgtac ttgttacctt
     3601 atgagttgga ctgctagtag tagaacagcc aaatattatt actacattta gtaaaaacaa
     3661 aacattcttt ctatacaata aaatagcgta tagagaggat gtttttaatt tgaaaaggag
     3721 tggttatctt tatgaaaaat agatcatttg ctatattagt attatccgta attttattag
     3781 atatattgtt aataacatta gctttaaaaa ctttaaattt agtaacaagt tttgtcatta
     3841 taccagtaat tataggaatg tctatttatt tcatcaaaaa aaataaacaa tttttttgaa
     3901 tgaaattacg ggcgaaaata atcactcaaa attattaata aaaacgaatc aactactttt
     3961 tctgaagaaa catacaaccg taaacaacaa ttgttgttta cggttgtaaa ttatcaaact
     4021 atatggtgac ttgttgctta aaataggttg aaatttcgtt tatttttttt gagaaatttt
     4081 acccacgtac tacccatgta cctatccacg tattacccat gtacccccca tgacaaatgg
     4141 gcggaggggc ttgcggcgct tggtattaaa ggcattatcc gcctcccagg tttctgacca
     4201 ttttctctca acgcggtaaa aaccccactt atgagagaaa atggtcagaa acctaaacga
     4261 tcggcaaaga aaacatacca agcgccacaa gcaaaaaaag ttagagtaac ttccagaaaa
     4321 gaaaaatttt tagaacaact tttgaaaatt tgattttgat ttatatccgt tcaatttaaa
     4381 catttgataa actgaaggct agtatttaaa atgtgtatac aagtggtagc gttaaaaaaa
     4441 gtggaggtat aacctatgtt aaaaaaaact gtgttggtac tgggttctat ttctctagga
     4501 atattaggtt ttagccccat gctattatcc gtaattggaa aaaacaattc caacagagaa
     4561 cttagattaa attcaaatta ttacaacagt cctaacgaag aattgtttgt aaaagagacg
     4621 gataaataat aaaaaagact gctcttccga gcagccagtt aaaaaaataa tgagagttta
     4681 ttcttgaaaa agcaacgcgc ttataaagcg ctaccaaaat tttatcatat tttataaaaa
     4741 ataacaatcc agtaaaaaat gactggtaaa aattcacaaa gaaacggaaa gaagcaacta
     4801 cagaaaatca agccttaaaa agagcttttt acctagacca attaaaaatc acaaagtgag
     4861 cgtctctatc cattggtaga ggggcaactt ggtctagtcg acattatgtc aactatgcaa
     4921 ttaaataact aactacttca tattagctta ataaaataat caaaatcact aggggaaaaa
     4981 atattgtata aaaattttca tttttaaata agaatagtaa atataaaaat aatttaagat
     5041 atctattact attaatccag atagagcaaa ccaagtaaat attccgtgtg taaagctaaa
     5101 aaattcattt gaatcaattc aaataaacct aatatagtaa caaatatatt aaaaataata
     5161 gctgtcaata ccagtaaatt attttgttta tttttaaatt atcttcattt aaaatagaat
     5221 ttatgttagt actataaaat tcacaaattt ttttagtgtc tctatatctg gtgttgtttt
     5281 gttattttcc cactttgaaa tagtttgacg tgtaacatta aattttcttg ccacatattc
     5341 ttgagtatac cctttttttc actcttaatt ctcttagctt agatcctaaa tcctccattt
     5401 tttctcctat gtaaacgatt atttacataa aattttatca taaaaatact aacgcccaaa
     5461 aaagttcttt attttataaa acacactaaa ataaaataat tctagtaaaa agataggaga
     5521 aataagttat gaaaaatttt tatgtctttc tgctttaatg ggtgtcttat taactagtgg
     5581 aggaatagtt agtgctacag aagctttaaa tctagatgta cccgaagacc ataccgccat
     5641 ttacggagga ggaatggaag ctacagaaga aggattttct tctagtaaat tgagatatgc
     5701 tgctggcggc ggagatttca actgtggggt taatggattt aaagtttatg caaattacta
     5761 ccatgctaga gctaaacata gtgcaacagc taaaaatgga cgaggcggtc aagtacgatc
     5821 agttcagaaa gctggagtaa gagcttatgc aacatgcaat gccacactta ctggaaatac
     5881 tggttggtgg aatgtttatt aaaagagagt ttaaactctc tttttattta catttgaaag
     5941 gaaattcaaa atatgttaaa aaaataaacg ctgttttatc actttttgtc tgcattacaa
     6001 tactattctt ttctactaat gttataaatg gtataaagac ttctgaagcg ctatataaaa
     6061 ataccgctga aattcatatg caatataaag atgatatgga ctttaacagt tatataaacg
     6121 ctttgtatga aatatctaca caagaaaata ttaatatttc tcaatatagt tttactgctg
     6181 ttaaccaact aactattctt gctacaaacc ctaacgctaa caaacactgg aaattttcta
     6241 aaaaaaatat attagcaaga aacgattcta aagtccatta tactaataac aaaaattcta
     6301 agtaccaatt aaaacttcca aataattttt aaatataaat atttatcctt tttctcatgt
     6361 ggaaaatatt ggattaagtg aaatattata tgtacaagga aactcaaaca aattaattcc
     6421 tgtactgaaa aatatggaac acctactatt tccaatatca atcaatcaga tacttttcaa
     6481 attaatacta atattgttct tgttatctgc tatcttagta tatttattgt aattactaca
     6541 attatatttg cttttcaaaa atgaaagaaa tcactctaaa aaaatgttag gctataactc
     6601 ttttgatact ataaaagatt ctattaaaga gttaaacctc ttattttgtt cttttgatta
     6661 taactttcat tttatctatt ttgagcatat attacagtaa cgaaaatttt ttcctatcat
     6721 ttcttctatg tagtattttt cattttataa ttagttttta tttatcatag ttactctaag
     6781 tatctatcat ttatctagta taaaaattat ctaaaaattc acgaccactt aagttaaatc
     6841 tttgtatatt aaatatctgt ctattcttaa gcatgatcct tctgttaata gcttctacaa
     6901 aagtaataaa tatccataat gaagccgaaa ataattctct taaatattgg gaaagaacta
     6961 ctaatttata taaaacaaat attactaatc aattaaatag aaacaacacc gtagaagaaa
     7021 ataactactt gaaaaaagct agtaaatttg tttataaaat acagaagaat tataaaactt
     7081 ttattatcgc gccatacaat tacgcaacta tccaagaaaa taataaagaa ttttttattg
     7141 gacaaaaagt ttatccaaat tttgaagact atgttagtca acctgctggt gcaggaatat
     7201 gtgttgattt aaactatcta aaatattaca atcctatttc ttttgaagaa tctactgact
     7261 taaatttaat aaactctgat ccactaacta cttacattct agtacctaaa aaatataaag
     7321 aatatgcaaa aatgattgaa aaaaattatt tagaagacat acagtttaga cttgaagact
     7381 ctcctcctca agctccttat aaaactccta atttgaaaaa tttaaaaata aaactaatat
     7441 ttgttaataa taaccaaaag tattttagtt ttaatacctt atatggaaag gcaaaagata
     7501 actatacaat aactgatccc atagttagag ttattaatcc agaaataaca gaatcattat
     7561 tttggggaaa tatattgact agtgatggtg gattattttt tgatcagaaa catacttcaa
     7621 accaaaactt tttaatcaaa ttaatccgta tataaaagaa tttaacctag aaaatatcat
     7681 aaatttttct tattctgttt ttcaagaaac tggtgaatta ttatctcaaa aaagtaaata
     7741 aattaaatgc atatctacaa tatcttttaa tattaggatt atttataata attaattttc
     7801 aaatgatttt tgttagtttt actataaatt caaaaaaata gctttaaaaa aatatttgga
     7861 tattcaccaa taagtcaagt tttagattta tgtttactcc cttttacaat agagttattt
     7921 gctactatgt gttttcaatt gatattttca aaaaacactt acttgtattt tataattttt
     7981 atgatctttt taataaatct aattacttct attattattt ataaaaaaat agttttaaat
     8041 attttaagga gaatatttta tgatattatc agttgagaat ttcaccctgt caattaataa
     8101 aaaaaagatc aaacaaaatt ggaacttttc aatatcagaa aaagatttta ttatagtaac
     8161 aggaaaaagt ggtagtggaa aaactactct gttaaataat ttaagcctaa tagataaaca
     8221 atataccggt aacttattct attttgaaaa gaaagtttct aaacatgata tacaacgtct
     8281 acgaagaaat catattagct atttatttca aaactatgct cttcttgaaa atcaaacagt
     8341 acaatacaat ttcgaattag caaaaaagtt taacagtaat atttccaatg atcatattta
     8401 taatttatta acagattttt ctttgccaaa agatatctta aaagaaaaaa tctttctctt
     8461 aagcggtgga gaacaacaaa gaatagcatt aattagaaat atgttaaaac catttgatat
     8521 attatttgca gatgaaccta caggaaattt agataaagaa aactcaactt ttataatgaa
     8581 ctttctgaaa aatttaacag aaaaagaaaa aaaagccgtt gtactagtaa cacatgactt
     8641 aaatgttcta aactatggaa ctaatattat agaattataa tttttacccg tgatgctagc
     8701 tgatttaaaa ctagcaccac gggtatttat tttatattcc atctctccta tcccaaaatt
     8761 taaataatca cttaataaaa aggaggcacc caagtgattt tattgaaaaa agagtatgaa
     8821 aaattttatg atagacttat ttattctttg caattgccca aaaaattaaa ccgtcaatta
     8881 caaaagactt atgaaactaa atcaactgaa gaatgggaga gttattgcta gatttttggt
     8941 agaagtaaca aaaagtggac atattatcat aatgactcgc cgaaagtctg ataaaaaatt
     9001 ggctaaaaat attgctgaat gtttaataaa actatcagtt ttagctaaat agttagtagt
     9061 ttacacaaaa aagcatttat gttttttgtt gcaaagcaac aatttcatct tcggtgaaat
     9121 aagcgaaact ttacaaaaag tggctagcat ttgctagctt tttttgtatt gtggagcgtg
     9181 ggttttgagg gggattcaag ggggagcttg cgcccctttg acaagcggtt atttgcacca
     9241 aatacggagg tatttggcta gcaaatatag agcttgccaa acccttttag tgagctttac
     9301 tcattgtacc tttttaggta caatggtatt gcgaacgata cgtccgtagg acgtactgaa
     9361 agggtgggac ttatgagcga acaaaatcaa aatttggcta gcaattattc taaaaaatat
     9421 acctatcgat ccgagccaaa acaaatcagt tttcgagtga gcgaatccga atttgcaaag
     9481 ctgaagcaat cggctgaagc tttgcaaatg agtgtgccgg ctttcgtgaa agccaaggca
     9541 caaaacgcgc gtctggtgac accgaaagtg gcgccagaca ttgcccaggc aatcgcgcgc
     9601 gatttagcca aagctggagg gaatatcaat caaattgcta agtggtgcaa tacacaccaa
     9661 catgacgttg cttctggtga cgctcaacgt ttatccgaaa acctaaaaat catgcaaaag
     9721 gagctacaaa agatatggca acaattaagg taagccgaac cacttcttgt agtcgtgcaa
     9781 ttaattatgc agaaccacga gcaaccatta aaacaggaat aaactgtgac gtcgattatg
     9841 ccaaaagtga aatgaaacaa attcgcatgc tgtatggaaa agacgaccgt gtacaggcac
     9901 acttattgat tcaatctttt cgaccaggag aaattaccgc tgaaaaagct aatcaactag
     9961 gggcagagta tgcaaaaaaa atagcccccg atcaccaaat agctatttac acacatacag
    10021 acaaagacca tattcataat cacattgtca ttaactccgt taatctggag actggaaaaa
    10081 aatttcaagc tcatggtcaa gcatttctaa acaaatgtta tgacctaaat gacgagattt
    10141 gtcttgcaca taacttacca atcactgagc gaggaaagaa gccagaaaaa cgaaccatgt
    10201 ctgaaataaa attaaaagaa aaaaatgaac ctgtttggaa agacgaaatt cggattgcga
    10261 tcgatcaaac aatgaaaaac aaaaaaacca gaacctatga ccaattttgt aattccctca
    10321 aaacattggg gattcattgc ttcaatcgcg ggaaaaattt cacttacgaa ttgatccaca
    10381 aaaagaaaaa agtacgatcc aacagactag gaaaggacta cgaaaaggag acgattttac
    10441 atgagctgga cagacgagaa cgaacctata accaacagcg aaccaacgag ctacgaagag
    10501 ctcgagctag cattcgcgaa actgcagaag aagtacgaag agaacaacga actcttacgc
    10561 ctgaacaacc ggaaattaga ccggctcgaa gaaaccatga acaacacgtt gaaagaggaa
    10621 cagaccacca acaagaatta tcgctctaaa acgttaaaag acatagaaaa cgaaataaag
    10681 aaacaacgtg cttttctcgt tccttatacg caagaaagga tacaaactac gattgattta
    10741 caagttaaaa aactccatca agagctatta gaccagcgaa ataaggaaca gaaaaaggtg
    10801 cttattcaac gatcattgga ttttcttgcc cccttatgtt gcttagtcct gacactgttc
    10861 gcattttggt tagtatgggg aggctaaaat ggcattttat ttaccctatc tactcatttt
    10921 tgttagtatt tcaggaagta tttggctcat ctataaaatt tttcaaacaa gatattcatt
    10981 aaaaggctct agaattcgat ttaagcgttt tttattacta ggggttgtat ttaccttatc
    11041 aatcgtttcg agcggattat tcggcgtttt agagggcaat aagcgcgttt ctagaagtgt
    11101 tttattagaa aatgtcactc aaaagtatga agtagcacat gagaaaaaga aaaaagagca
    11161 agtattagca caaaaaatag aaaaatttac cgcttgttat gaggacatga atgatatttt
    11221 tgtgaagcaa gaaaagcgtt tgacggataa aaatatggaa acactcactc gactttatcg
    11281 gaatttgcca gaagaacagc aaaaagagta ccaagaaaaa tatgaacaag taaaaaaaga
    11341 cgtgcaatat gtgaaagata cgaaagtgga agaagcttgt agtgatttat ttggagatac
    11401 aattcctttt tcaactagcg aacaagaacg aaaagaacga caacaatcag tcacatatga
    11461 acgctatgaa atcttgcttc aacaagcaat taacatacaa aatctgacta aaaaagaaac
    11521 agctttaaat tatttaaaaa gcgtcaagga atggttagat cagcaacagc aaaattgaat
    11581 ttgattgaaa ttcatgttaa actaaacatg caaatgttca aacattagat acacgaaagc
    11641 cccagagagg tcggattctc tggggctttt tttaggttga tgtcgccttt actgatttta
    11701 cttcttgttt cggttattca accaataact gaatacagag atacaaactc ctacaagaag
    11761 ggggcaaata aacagttcaa acatcagata cacctccttc cagtcgcaag actgtcggat
    11821 aggcgacaag ttattatacc ctacaaaatt aaatctgtgt ggcaatttta agtatctctt
    11881 atttccgggt tgttagggct tgccctgacc gcctgtaggg cgctttaaat tccagataaa
    11941 ggtttttata ctaagactgt cttaaaacgg cttagacaag ctcttagagc ctcaaccctc
    12001 ctttttgaac aatggtttta gcaaaggagc tggaggcgac tgcgcaggta taatgtgaga
    12061 gcgtagcgat cacacctttt tggcgatcgt agggagcgag gttttgaatt agcagtttag
    12121 aaaagttcgc gaacctgttt aaagaaaaaa taattgtttt tttaggtggg aaattttgtc
    12181 tccctctttt ataatctttt tttaaacctc tttttaaacc tctttaccgt cggctgtgtc
    12241 cttactcccc caagggatac aaaattttat aggcatgaaa ttatctgttt ataggcatga
    12301 aattatctgt ttataggcat gaaattatct gtttataggc atgaaattat ctgttttagg
    12361 tattgttttt agacataata tgtcctaaaa tgatgtcaat aaaatcattt agactggagt
    12421 aacaactaa
//
