LOCUS       NZ_CP092518             4655 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Klebsiella quasipneumoniae strain EF262 plasmid p6, complete
            sequence.
ACCESSION   NZ_CP092518
VERSION     NZ_CP092518.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26096228
            Assembly: GCF_023920245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Saito,R. and Mahazu,S.
  TITLE     OXA 181 producing Klebsiella spp
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Saito,R. and Mahazu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2022) Department of Molecular Microbiology, Tokyo
            Medical And Dental University, 1 Chome-5-45 Yushima, Tokyo 1138510,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2021
            Assembly Method :: Unicycler hybrid assembly v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 53.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 03:44:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,551
            CDSs (total)                      :: 5,429
            Genes (coding)                    :: 5,291
            CDSs (with protein)               :: 5,291
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 45 of 138
            Pseudo Genes (incomplete)         :: 87 of 138
            Pseudo Genes (internal stop)      :: 30 of 138
            Pseudo Genes (multiple problems)  :: 20 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092518.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="EF262"
                     /isolation_source="HVS"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="p6"
                     /geo_loc_name="Ghana"
                     /lat_lon="4.9239 N 1.7446 W"
                     /collection_date="2018"
                     /collected_by="ENRH_Hospital staff"
     gene            complement(373..448)
                     /locus_tag="MKM89_RS27710"
                     /old_locus_tag="MKM89_27705"
     tRNA            complement(373..448)
                     /locus_tag="MKM89_RS27710"
                     /old_locus_tag="MKM89_27705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(413..415),aa:Arg,seq:tct)
                     /name="tRNA-Arg"
     gene            complement(629..871)
                     /locus_tag="MKM89_RS27715"
                     /old_locus_tag="MKM89_27710"
     CDS             complement(629..871)
                     /locus_tag="MKM89_RS27715"
                     /old_locus_tag="MKM89_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074196216.1"
                     /translation="MSKESELEFRTKELERQMKGVQRRIEVVNAKYDSQTKKQERRIRD
                     LEIKTAVQSGVTQREVANIYELSPGRVNQIIKKVG"
                     /name="hypothetical protein"
     gene            1261..1656
                     /locus_tag="MKM89_RS27720"
                     /old_locus_tag="MKM89_27715"
     CDS             1261..1656
                     /locus_tag="MKM89_RS27720"
                     /old_locus_tag="MKM89_27715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_126066683.1"
                     /translation="MNANEVKDILEHSNHDDWIVDDETGSFTYKNDLNLHIKRADYDNY
                     NSFSEPWAVSHPNPNACSVQYTVNYGSSFVDRKTLVSVDGHRATLPMPASANDLTVTSA
                     DVNFAKIVDTGERVDEYILRSGLKVQP"
                     /name="hypothetical protein"
     gene            complement(1746..2297)
                     /locus_tag="MKM89_RS27725"
                     /old_locus_tag="MKM89_27720"
     CDS             complement(1746..2297)
                     /locus_tag="MKM89_RS27725"
                     /old_locus_tag="MKM89_27720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040173808.1"
                     /translation="MARVHAIKRSGLHIDIDYVVHKKIALDRAVILRYSTKNPDYHADF
                     VGLSEPEIEREKDLILKENDTTHCLLLLTAIEAIFRLDADQKYCNKKRDPLSRKIKEIY
                     DKTKGEKIKLDDDLINTRMNEDDARLKKDYNDLKTCFKFRHWLAHGRYWHPKRIKNFYF
                     DSILIVIDSILNSTKYNFEV"
                     /name="hypothetical protein"
     gene            complement(2290..2856)
                     /locus_tag="MKM89_RS27730"
                     /old_locus_tag="MKM89_27725"
     CDS             complement(2290..2856)
                     /locus_tag="MKM89_RS27730"
                     /old_locus_tag="MKM89_27725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064279389.1"
                     /translation="MNMKTLKDIIEKQKKEEEEQKFDPAEQIEEYISLVEKLYTITLES
                     IDELIKDGLIKQSRKKISINEESLGDYEIDSLILTINSSKIKFEPVGTMLIGSKGRVDV
                     TGPFGKEKFILIRKGVKSPSDLIKVRVNVVGAYKPEKDKTDEDVKKPSIDDWEWKTMPS
                     DSRWVKFDDVNSETITNIIMRMING"
                     /name="hypothetical protein"
     gene            3652..3843
                     /locus_tag="MKM89_RS27735"
                     /old_locus_tag="MKM89_27730"
     CDS             3652..3843
                     /locus_tag="MKM89_RS27735"
                     /old_locus_tag="MKM89_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044525164.1"
                     /translation="MSKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3843..4244
                     /locus_tag="MKM89_RS27740"
                     /old_locus_tag="MKM89_27735"
     CDS             3843..4244
                     /locus_tag="MKM89_RS27740"
                     /old_locus_tag="MKM89_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3124..3531)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 attgccggcc gtcgacgaac ccgattcctg atcgtgctga aaaccggttt tccggctgcg
       61 ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatggga
      121 agaacgccgg aaacaggact taccctggct gaatggcgtg aattttcgcg ctatgcactt
      181 gcgtgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg
      241 tggtttgttg cctgttttac cggtttcccg tcagaaacgc cctgaggccg ttttagcggt
      301 gcgcgtaatg cgacgttatg gtaaatgctg tggccagaat agttgcctcc ccccttttag
      361 gggggaggct tttggtggag acaatgggat ttgaacccac aaccatcaga ttagaagtcc
      421 gctgctctac cgttgaagct atatccccat ctggacccaa caaagtcggg accgtctttg
      481 atttaacatc actgcatgct ttttgtgaag ttttttatgt gtttaccggt cgcatcagaa
      541 cagggtatgt aatgagacct tatggtaaaa agcacgtcag tttgtattgc gagaaaaaac
      601 cgccttttgg cggttttttc tctagagatt agccgacttt tttaatgatc tgattaacgc
      661 gacccggcga cagttcgtaa atgtttgcaa cctcacgttg cgtaacgcct gactggactg
      721 ctgttttgat ttccaaatca cgaatgcgac gttcttgctt cttggtttgg ctgtcgtatt
      781 tggcgttaac cacctcaata cgtcgctgga cacctttcat ttgacgttcg agttcttttg
      841 ttctgaactc tagttctgat tctttactca tatgatatac tcgctaagta aggttattgt
      901 tgggtgtcat tcgaaggttt cttcttgccg gaaggtctta acctatgttt gacaaatcaa
      961 caatagcctt tttttacgat cacagcaact tctataatca gtgtttcccc ttatttttct
     1021 ttgtttttat tttccaataa aaacaataag ttgcgattgg tttaattctg atattaaaaa
     1081 ggctaaacac acctatttta tgtatatgat ttttaagtga aaaaacaaat cgatgtttag
     1141 tatttattat ttgattcttt actggaaaat ttgattgcac atagagaact tttcggagca
     1201 gtgtggtgta gaactcaatc gatctttaca ttctaaccat tgatttatat gggggtatgc
     1261 atgaatgcta atgaagtaaa agacattctt gaacattcta atcatgatga ttggattgtg
     1321 gatgatgaga ctgggtcgtt tacatataaa aacgacttaa atttgcacat taagagagcg
     1381 gactatgaca actacaactc ctttagtgaa ccctgggctg ttagtcatcc gaatccgaat
     1441 gcctgttcag tacagtacac cgtaaactat ggttcatcat ttgttgatag aaagactttg
     1501 gtttctgtcg atggtcaccg cgcaaccttg cctatgcctg catccgcaaa tgatttaacc
     1561 gtgacatcag ctgacgtgaa ctttgccaaa attgtagata ctggggagcg tgttgatgaa
     1621 tacattctcc gctctggtct taaagttcaa ccatgattta aatgtgagcc agcaatctaa
     1681 aattgctggc tcataatgtg ctgtgcttaa gggacgttat ggtgactttt tttaatgtca
     1741 ctgacctaaa cttcaaaatt gtatttggtt gagtttaaaa tggaatctat cactattagt
     1801 atagagtcga aataaaaatt ctttattctc tttggatgcc agtatcttcc atgagcaagc
     1861 caatgcctga atttaaaaca tgtttttaga tcgttgtagt ctttttttaa tctagcatca
     1921 tcttcattca ttctggtatt tattaaatca tcatctaatt ttattttttc tccttttgtt
     1981 ttatcatata tctcttttat ttttcttgat aatgggtctc tttttttatt gcagtacttc
     2041 tgatctgcat ctaatcgaaa tattgcttca atagctgtta acaataaaag gcaatgtgtt
     2101 gtgtcatttt cttttagtat taagtctttt tctctttcaa tctccggctc acttaagccg
     2161 acgaaatcgg catggtaatc tgggttcttt gttgaatacc gcagaattac agcacgatcg
     2221 agtgctatct ttttatgtac gacatagtca atgtctatgt gtagaccaga tcttttaatt
     2281 gcatgaaccc tagccattta tcattctcat aataatattc gtaatggtct cggaattcac
     2341 atcatcaaat ttaacccatc gactatcgct aggcattgtt ttccattccc aatcatcaat
     2401 tgacggcttt ttcacatctt catcggtctt atctttttct ggtttatatg ccccgactac
     2461 gttaactctt acttttatta aatcgctggg gcttttgact ccttttctga tcagaatgaa
     2521 tttttctttt ccaaatggcc ctgtgacgtc aacccttcct tttgaaccta ttagcatggt
     2581 tcctactggc tcaaatttta ttttacttga gtttatagtg agtattagag agtcgatttc
     2641 ataatcacct agagactctt catttattga tatttttttt cttgattgtt ttatcaatcc
     2701 atctttgatt agttcgtcaa tagattctaa tgtaatagta tataattttt caacaaggct
     2761 tatgtattct tctatttgtt cagctgggtc gaatttttgt tcttcttctt cttttttttg
     2821 tttttcaatt atatctttca gcgttttcat gttcattttt tgtaacctct agatcggaat
     2881 tattttacga ttctagcctt tgctttggtg tcgagcaatg tgttgttctt gacgcttaac
     2941 ttgctcggag gtattgtcgc ttctgaaacg aaaaaaccgc cttggcgggc ggttttttcg
     3001 aaggttcata gagttggcgc tcgttgaacc gaggtaactg gcttggagga gcgcagtcac
     3061 caaaacttgt tctttcagtt tagccttaac tggcgcataa cttcaagaca aactcctcta
     3121 aatcagttac cagtggctgc tgccagtggc gcttttgcat gtcttaccgg gttggactca
     3181 agatgatagt taccggataa ggcgcagcag tcggactgaa cggggggttc gtgcatacag
     3241 tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagataaacg
     3301 cggccataac agcggattga cactggtaaa ccgaaaggca ggaacaggag agcgcacgag
     3361 ggagccatca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg
     3421 atttgagcgt caaattctgt gatgcttgtc aggggggcgg agcctatgga aaaacggccg
     3481 ccgtgcggca tttccttttc cccgtcacgc taccgctcgg ttttctcgag ctcttgtacg
     3541 catactcctg ccgctcgccg cagccgaacg gcaggagcgt agcgactgag tgagcgagga
     3601 agcggaaaag agaacatacc tacactgagc actgacgctc tgaggcctct tatgtctaaa
     3661 caacagaaaa cagcgctgaa catggctaaa ttcatccaga atcagtctct gctcctgctg
     3721 gagaaactta acgagcttga tctcgatgtc gaagccgacc tttgtgagaa actccacgat
     3781 gatgcggagc atcttttccg tacactgtcc tcgagactgg atgcattaca ggatggtaac
     3841 tgatggccag agtgtctatt agtgaggctg cccgactgac gggtaagagt agaacaacac
     3901 tgcacaggtt aattaaagca ggtgacctgt ccacctgtag cggtgagcgt aatgcaaaaa
     3961 tggtagatac ttcagagctt ttacgtgtct ttggtccctt tgagcaaccg aagggtgaac
     4021 accattctgg acaggtctct ggacagcgtg atacagcctc tgctgaccag agtgaacagg
     4081 tgattcgtca tctgaaacag gagattgaac acctgaacac cttacttttg tccaaggatt
     4141 cgcacattga cagcctgaaa caggccatgc tccttcttga aaatcaccag gcaaaattaa
     4201 ctgaatcacc ggcgccatgg tggaaattct ggaaaaaatc ctgaccccgt catgcttcag
     4261 ttttcctctc cggccatccc ggggagttcc tgaaccttcc caaaaatcgt cagatctctg
     4321 aacgctcctc accactaaaa atacccacac cccacgcaaa attaaaattt tgaagataac
     4381 tcattaatat cagttagttg tgggttatta aattacgcag tattactttc tgtaaggtga
     4441 atccaagtcg tcggcgattc gtgtacgact tgccgacgac tcgtcataga gtcatcgacg
     4501 actcacataa gactatcagt aacttatgac gacttgactc tagagtcgaa tcgtcatttt
     4561 ggtgtttttt tgaccttgac gactctagag tcaagtcacg agtcgaatcg ccggtgaatc
     4621 gtgtggtgag tcgtccgtcg acttgccata acgtg
//
