LOCUS       NZ_CP092517             5748 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Klebsiella quasipneumoniae strain EF262 plasmid p5, complete
            sequence.
ACCESSION   NZ_CP092517
VERSION     NZ_CP092517.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN26096228
            Assembly: GCF_023920245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Saito,R. and Mahazu,S.
  TITLE     OXA 181 producing Klebsiella spp
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Saito,R. and Mahazu,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2022) Department of Molecular Microbiology, Tokyo
            Medical And Dental University, 1 Chome-5-45 Yushima, Tokyo 1138510,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2021
            Assembly Method :: Unicycler hybrid assembly v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 53.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 03:44:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,551
            CDSs (total)                      :: 5,429
            Genes (coding)                    :: 5,291
            CDSs (with protein)               :: 5,291
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 45 of 138
            Pseudo Genes (incomplete)         :: 87 of 138
            Pseudo Genes (internal stop)      :: 30 of 138
            Pseudo Genes (multiple problems)  :: 20 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092517.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="EF262"
                     /isolation_source="HVS"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="p5"
                     /geo_loc_name="Ghana"
                     /lat_lon="4.9239 N 1.7446 W"
                     /collection_date="2018"
                     /collected_by="ENRH_Hospital staff"
     gene            668..1300
                     /locus_tag="MKM89_RS27675"
                     /old_locus_tag="MKM89_27670"
     CDS             668..1300
                     /locus_tag="MKM89_RS27675"
                     /old_locus_tag="MKM89_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689667.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_249108301.1"
                     /translation="MTSRVYAYLRASTKEQDASRAREQLDAFAHSKGMTIATYFTENES
                     GASLQRPELFRLLDIAQKDDVLLVEQVDRLSRLKADDWQVLRQTISSKGIRVVALDLPT
                     SHQLIQSGDEFTNRMLEALNGMMLDMLAAIARKDYTDRKRRQEQGISKAKEAGKYKGRP
                     EDAQRNEKIARLLNAGMSYTDIMGTVNCSRATVAKVSKSLKETGITG"
                     /name="recombinase family protein"
     gene            complement(1536..2198)
                     /locus_tag="MKM89_RS27680"
                     /old_locus_tag="MKM89_27675"
     CDS             complement(1536..2198)
                     /locus_tag="MKM89_RS27680"
                     /old_locus_tag="MKM89_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008320537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="WP_048269942.1"
                     /translation="MNHAVTSHYDNGRYHLINSANYAFGDNTYSLNFKPDSKRYSPIRQ
                     EIMDSFITQLCAMQSHYSKIFAFRFDLSVPAGMPVAESNQLISELFTRLRGKFTAKRWN
                     GQPIKKFAYGWVREKEKAKQVHYHCWIALPHFQVRVAGFGNEGTYGLINTLWSELTAQK
                     GRSRPCNGTYTINRDDHSSLVALVERVSYLAKTRGKYSTGDGQRMFSASKLKILTAA"
                     /name="inovirus-type Gp2 protein"
     gene            complement(2327..2470)
                     /locus_tag="MKM89_RS27685"
                     /old_locus_tag="MKM89_27680"
     CDS             complement(2327..2470)
                     /locus_tag="MKM89_RS27685"
                     /old_locus_tag="MKM89_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_177225380.1"
                     /translation="MTVTKKGPPCPAYIDPEALEKAITAARKIREANPERYKSIPVQPK
                     LF"
                     /name="hypothetical protein"
     gene            complement(2467..2970)
                     /locus_tag="MKM89_RS27690"
                     /old_locus_tag="MKM89_27685"
     CDS             complement(2467..2970)
                     /locus_tag="MKM89_RS27690"
                     /old_locus_tag="MKM89_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249108303.1"
                     /translation="MTKKTAHRAAESDLQAFMNDAADRFKPDAAVLAARIDTAVQRYEV
                     TSTTQRFNAPAPLAMQQLQERILEGWRYDIGIPQSVYVAGTGNMSITLRKPASLVEQEI
                     ADLKRQVEEGYHNELAAAMEREVDKLVQDAANEAQRRAEEAAAAERDAMRQRLRDMLLS
                     GATV"
                     /name="hypothetical protein"
     gene            3573..4028
                     /locus_tag="MKM89_RS27695"
                     /old_locus_tag="MKM89_27690"
     CDS             3573..4028
                     /locus_tag="MKM89_RS27695"
                     /old_locus_tag="MKM89_27690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_180264771.1"
                     /translation="MSKQSQNLLNLTQAAEAVGITRKTLYSHIDKGLVSVTRQQGKRYV
                     DVSELIRHYGSVSLPEEKVNGVTRSQTIHNDDAILAMQRELVELRKLIEKQQLLLEDKQ
                     EADLLREQMTGLQNERDAASLELEKVKVELEAERKKGFFRRLFKHLH"
                     /name="helix-turn-helix domain-containing protein"
     gene            4115..4447
                     /locus_tag="MKM89_RS27700"
                     /old_locus_tag="MKM89_27695"
     CDS             4115..4447
                     /locus_tag="MKM89_RS27700"
                     /old_locus_tag="MKM89_27695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071739097.1"
                     /translation="MVDIFGNKTKQLLADAQRLLEQAQESMSEDIEKLKEQQIATDARL
                     ARLERQQDESLQLIGNLQNSVDTMRDSAIRGEVASGQKSKDVAVKYGITPSRVTQIAPR
                     RRYNNG"
                     /name="hypothetical protein"
     gene            5154..5720
                     /locus_tag="MKM89_RS27705"
                     /old_locus_tag="MKM89_27700"
     CDS             5154..5720
                     /locus_tag="MKM89_RS27705"
                     /old_locus_tag="MKM89_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043876049.1"
                     /translation="MAKYQIDSIQNWPSFEHDGSTYSLSHLDAHEITYKGDKQDFTFVV
                     TYGLHCFAKEDTPYVIPVVYQDGREEKTICMERYEASKHIRHILVNLPDMRLFHTASEK
                     YFTLDMMNSATGQLEPYKICIAFYKEKRLLRMHILSAFFVRYGPGFPGEPIPNKAVSLF
                     KVATDTASRQKKHAWIPKEANNRIK"
                     /name="hypothetical protein"
ORIGIN
        1 tttgaccaac aggaaaagtt gaagctgagg gtgttttttc aggcttagaa agtggcgacc
       61 ccctttcggg ggtctcagcc gccataaggc ggggcaggac actttaccgt catggacgaa
      121 cctgttacca ggaagtggga tgcgatttcg ttaccgaaat ccctgtaacc atccttaaag
      181 caattgtagg tagtgtgatg tagatttgca accaacattt tggttgcacc tatatactaa
      241 cttttctcga tctccccgca accccttgct gtatctggct ttcttgaaat agccgactaa
      301 agagaaccta aagcctaacc tgatatcacg tgttgcatat ttataacccc cctccggcgc
      361 gagatcgctg atacccccac catacgtaaa ccggctggct gcaaatatcc tgccggtgat
      421 tgtacggctg gcgcatgggc tgaggtgtta ggcggatgtt ctcccccttc acgatttacc
      481 gccgatcttt tcaaaatatc tgccggtggg ttgatcgata tgctggctgt catgcggtct
      541 ggtttaggta tacctaaaga ggcactggtt tattgaacca gtcaggccgg gaaaaacatt
      601 tattgaacca ctcttaatag actgttatta ttgaaccact aaaaacagac tgataaggga
      661 taccgccatg acatcacgtg tttatgccta cctccgagcc tcgactaaag agcaggacgc
      721 atcacgcgcc agagagcagc ttgatgcgtt cgctcattct aagggtatga ccattgccac
      781 ctatttcaca gagaacgagt cgggagcgtc gttgcagcgc cctgagttgt tccggttgct
      841 ggatatcgca cagaaggatg acgtattgct ggtggagcag gtagaccgcc tgagccgctt
      901 aaaggcggac gactggcagg tattgcggca gaccatcagc agcaagggga tcagggttgt
      961 agcgttagac ttaccgacca gccaccagct aatccagtcc ggcgatgagt ttactaaccg
     1021 tatgctggaa gcattgaacg ggatgatgct ggatatgctt gccgccattg cacgtaagga
     1081 ctacaccgac cgtaaacgtc gtcaggagca aggaatcagc aaggcgaagg aggctggcaa
     1141 atacaagggt cgcccggaag acgcccagcg caacgagaag atcgccagac ttctgaatgc
     1201 tggcatgtca tacaccgaca tcatgggaac ggtgaactgt tcccgtgcga cggttgccaa
     1261 agtgagcaag agtctgaaag agaccggcat cacgggctaa cccgccttcc acaatccaga
     1321 gtgccgacct gagacggtcg ggtgtattga gatcctcgcg atgtgcgggg atttttttat
     1381 taaataacaa tcatttagat tgatttaatg tgagaaaaac ggcggcgcgt ggatcgctgt
     1441 gggcgttact gtgcctgctg tcgtcaaatc tgtcatggga aagaccccag atatttttcg
     1501 tgtgcatatg gctggcccgg tggaatcgaa gccggttaag cagcagtcag tattttcaac
     1561 tttgatgcag aaaacatacg ctgcccgtct ccagtggagt atttccctcg cgtcttggca
     1621 aggtatgaga cccgctcaac cagtgctacc agcgatgagt ggtcatcccg gttgatggtg
     1681 tatgtgccat tacacgggcg tgagcggccc ttttgggctg tgagctcact ccaaagagtg
     1741 ttgatcaggc cgtatgttcc ctcattgccg aatccagcaa ctctgacctg gaagtgaggt
     1801 aatgcgatcc agcaatggta atgaacttgc ttggccttct ctttttccct tacccagcca
     1861 tatgcgaatt tcttgattgg ctgtccattc catcgctttg cagtgaactt gccgcgtagt
     1921 ctggtgaaca gctcactgat cagctgattg ctctcagcta ctggcattcc tgccgggact
     1981 gacaggtcga accggaaagc gaagattttg ctgtaatggg actgcatcgc gcagagttgg
     2041 gtaatgaagc tgtccattat ctcctgacgg attggcgagt agcgcttgct gtcaggtttg
     2101 aagttcaggc tatacgtgtt gtcgccgaag gcgtagttag ctgagttgat aaggtgatat
     2161 ctgccgttgt cgtaatggct ggttactgca tgattcaatg ttcctccggt tgatggttgg
     2221 gtatgtacag gtattaataa aaaccagtac ggttactgct ttagcaaagt ctgcctatgc
     2281 cgcgccactg ctgacgcgga gatgcaatta ttgccagcgt ttttactcag aaaagtttcg
     2341 gctggacggg gatggatttg tagcgctccg ggttggcttc ccggattttg cgagcggcgg
     2401 tgatcgcctt ttccagggcc tccgggtcga tgtaagcggg gcatggtggc ccctttttgg
     2461 tgacggtcat acagtggccc ctgacaggag catatcgcgg aggcgttggc gcatagcatc
     2521 acgctctgcg gcggcggctt cttctgctcg gcgctgtgcc tcgttagcgg cgtcctgaac
     2581 cagtttgtct acctcacgct ccatcgcggc ggcgagttcg ttgtgatagc cctcttccac
     2641 ctgtcgtttc aggtcggcga tctcctgttc caccagcgac gccggtttac ggagggtgat
     2701 gctcatgttt ccagtaccgg caacgtaaac ggactgcggg atgccgatat cgtaccgcca
     2761 cccctccaat atcctctcct gtagctgttg catcgccagt ggtgccggag cgttgaaacg
     2821 ctgtgtggtg ctcgttactt cgtaacgttg tacggcggta tcgatccgcg cggccagcac
     2881 ggcggcgtct ggtttgaacc ggtcggcggc atcgttcatg aatgcctgta aatcgctctc
     2941 agcggctctg tgggcggtct tctttgtcat ggggtaatcc ttctgtaatc gttgaataaa
     3001 ggccatagca ggccgctggc gctctctggt gagcgtcatt tgatgtgacc gtaatatgcg
     3061 gtcatctcgt cagtgtttgt gtctcaggcg gtcaggcgta ttcgccgtaa aggctctgtc
     3121 cgcaggccgg gcaatgctgt ggcgtatccg gtgtaaaccg gaaccatctg gtgacgtctc
     3181 cgcgtccgaa agtgaccccc gcgaggtggg tgacttccag cgccgtgagc gttctgtacg
     3241 cagtcgatag cggcaggtca acgcagcggc tgactgcttc tgctgtcact ggtacatcat
     3301 ctacggtatt caggtaatgg aggacgttaa tccggggatt agtggcacgg aggccgatgt
     3361 cattcaggat cgcggcgagg ctggcgcgtt ccgtatcagt cggggctgca aatgatggca
     3421 gcatgggcgt tgaggtcaat atttctcctg ttttatgtgt tttattggtc agtttccatt
     3481 ttatcacaga tgagaaactt tcgcatctac aggttggctg tttttttgta tactatgcag
     3541 agtaaacaca tctattttat ggcgggtata cagtgagtaa acagagccaa aatctactca
     3601 atcttacaca ggctgctgag gcggtaggta tcacgaggaa aacgctatat tcccatattg
     3661 acaagggctt ggtgagtgtt acccgccagc aaggtaaacg atacgtcgat gtatccgagt
     3721 taatcaggca ttacggatct gtatcccttc ctgaggaaaa agtaaacggc gttacacggt
     3781 cacaaactat acataatgac gatgcaatct tggctatgca gcgggagctg gtcgagctga
     3841 ggaagctaat cgagaagcaa caattattgc ttgaggataa gcaggaggcc gacctgctgc
     3901 gggaacagat gaccgggttg caaaatgagc gtgacgcagc atccttggag ctggaaaaag
     3961 tgaaagtgga gcttgaggcg gagaggaaga aaggattctt taggcggcta tttaaacatt
     4021 tgcattaaga aaaaatagat gtgattatcc accatgaaca gaaattaaat cttcctaaat
     4081 tttggctgtt ttttaatcct ggcgtggggt gtctatggtt gatatctttg gcaataaaac
     4141 gaagcagttg ttggctgatg ctcaacgtct tttagagcag gcccaagagt caatgagtga
     4201 agatattgag aagttgaagg agcagcaaat tgcaacggat gctcgccttg ctcgtttaga
     4261 aaggcagcag gatgagtctc ttcaattgat cggcaatttg caaaattctg ttgataccat
     4321 gagagatagt gcaattcgtg gtgaggttgc cagcgggcag aagtcaaaag atgtagccgt
     4381 taaatatggg attacaccgt cgagggtaac ccagattgca ccacgccgcc gttataacaa
     4441 tggttaagtt tggtggggtt gctcttgtga tcttcctctg ctcagcgtag tctcttgctt
     4501 agaaacgaaa aaacccacgt tctcaggcgt gggttttaac gggtaaacgg gttttctcag
     4561 gactcgtcta ccaggtaact gagtctttag cgggaacagc tacctaaaac gtgatctcag
     4621 tgtatccgcc ttacgcggag cattcaagct acagacgggc gtttttgtgc ccgaatgcat
     4681 atcaacaaag ttcccgcacg caagactcgt taccgtattg gctgttgcca ttagcggcgt
     4741 tttgccgtgt ccgctggggt tggaatcgtg agaatagtta cccagcaggc gcaacggtcg
     4801 gatgaaacgg ggagttcgtg catatcatcc agcttggagc gaaccgccta aaacgagcag
     4861 tgccagcata gggaagctga gagttagcgc ccccaaggta gaaagagcgg caaggctggc
     4921 tgcaaacgtc aaggcgggaa cacaggagcg cacgagggag ctactcatcg gaaacggtgg
     4981 ggatctttaa gctgtgtcgg gtttcgccac tcttgatttg agcgtctaaa ttgtgatgct
     5041 cgtcaggagg ggcgaagcct tctgtaaaag tcctggccct tctttttccg gtttccttcc
     5101 gcttgtctct tatctggtag aatatgcgga ttcgaccaac atggattgat gcagtggcta
     5161 aataccagat tgattctatt cagaactggc cttcatttga acatgatggt tctacttatt
     5221 ctctttcaca cctcgatgct catgagatca cgtataaggg tgataagcag gatttcacct
     5281 ttgtggttac ctatggttta cattgtttcg ctaaagaaga tacgccttat gtcatcccgg
     5341 ttgtatatca ggacgggcga gaagaaaaaa ccatatgtat ggagcgctat gaggcgtcca
     5401 agcacatccg tcatatcttg gttaatctgc cagatatgcg attgtttcat acggcatcag
     5461 agaagtattt cacgctggat atgatgaata gtgctacggg gcagcttgaa ccttacaaaa
     5521 tttgcattgc gttctacaaa gagaagcgct tactacgtat gcatattctt tctgcctttt
     5581 tcgttcgtta cggtccaggt tttcccggtg agcctatccc aaataaggct gtcagtttgt
     5641 ttaaggtcgc gactgatacg gcaagcaggc agaaaaaaca tgcttggata ccaaaggaag
     5701 cgaacaaccg tatcaaatga gatgcgtcag gtacattgat gaactggc
//
