LOCUS       NZ_CP092469             2569 bp    DNA     circular CON 12-SEP-2023
DEFINITION  Klebsiella michiganensis strain KO_408 plasmid pKO_6, complete
            sequence.
ACCESSION   NZ_CP092469
VERSION     NZ_CP092469.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25236446
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Saito,R., Nukui,Y. and Prah,I.
  TITLE     Genomic profiling of New Delhi metallo beta lactamase 5 producing
            Klebsiella michiganensis strain in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Saito,R., Nukui,Y. and Prah,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2022) Department of Molecular Microbiology, Tokyo
            Medical And Dental University, 1 Chome-5-45 Yushima, Tokyo 1138510,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 28-SEP-2019
            Assembly Method :: Unicycler hybrid assembly v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 66.0x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/11/2023 06:52:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4
            CDSs (total)                      :: 4
            Genes (coding)                    :: 3
            CDSs (with protein)               :: 3
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092469.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="KO_408"
                     /isolation_source="respiratory culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="pKO_6"
                     /geo_loc_name="Japan"
                     /lat_lon="35.7017 N 139.7643 E"
                     /collection_date="2018"
                     /collected_by="TMDU_Hospital staff"
                     /genotype="ST-265"
     gene            134..652
                     /locus_tag="L3249_RS00005"
                     /old_locus_tag="L3249_30970"
     CDS             134..652
                     /locus_tag="L3249_RS00005"
                     /old_locus_tag="L3249_30970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_264115269.1"
                     /translation="MREDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQ
                     VSISPEALEPRVLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARE
                     LQKTMDAQQKRLQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTR
                     SRGMGGMER"
                     /name="plasmid recombination protein"
     gene            complement(722..949)
                     /locus_tag="L3249_RS00010"
                     /old_locus_tag="L3249_30975"
     CDS             complement(722..949)
                     /locus_tag="L3249_RS00010"
                     /old_locus_tag="L3249_30975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024196437.1"
                     /translation="MNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRTS
                     GNKYSTKGLSPKQKNAAFSILKKHDYHLND"
                     /name="hypothetical protein"
     gene            complement(939..1442)
                     /locus_tag="L3249_RS00015"
                     /old_locus_tag="L3249_30980"
     CDS             complement(939..1442)
                     /locus_tag="L3249_RS00015"
                     /old_locus_tag="L3249_30980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_112015175.1"
                     /translation="MAKRGAGVNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPY
                     VKLYIADIAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKN
                     IIAALVDKQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVT
                     DDEC"
                     /name="hypothetical protein"
     gene            2126..>2557
                     /gene="mobV"
                     /locus_tag="L3249_RS00020"
                     /old_locus_tag="L3249_30965"
                     /pseudo=""
     CDS             2126..>2557
                     /gene="mobV"
                     /locus_tag="L3249_RS00020"
                     /old_locus_tag="L3249_30965"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     Typing          3..649
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.759"
                     /coverage="96.0"
                     /evalue="5.32e-122"
     Typing          2118..2569
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.04"
                     /coverage="96.0"
                     /evalue="7.879999999999999e-95"
ORIGIN
        1 atacgggaag gatcgtgttg tggcggcggt ggttcaccgg gacgaggcca ccccgcacct
       61 gtctgcgttc gtggtgccat tgacgcagga tggacggctg tccgcgaaag agtttattgg
      121 cgggcggtca aagatgcgtg aagatcagag cacctacgct gaatcagtaa aaaaactggg
      181 tcttgagcgc ggcatcgagg gaagccgcgc cacgcatcag acagttcagc actactacga
      241 atcgataaac cgtggcaccc gtagccaggt atcgatctcg ccggaggcgc tggagccgcg
      301 tgtattgcgg aagggcattt tcaccaaaga cgtggaagat caggcagcca tagccaaacg
      361 tctaagccac gcagtgaacg atggctttgc aggaaccatc gctatggcct ctcagagcgc
      421 ccagaacgca aaaagagcgc gtgagctaca gaagactatg gatgcgcagc agaaacgcct
      481 acagagcgtt acagagcctt ttaaggggct ttccagagag cagatgaccg aaatcctgat
      541 gatggctcag cggtttaagc agcaaaacca ggaaaaggaa aaacagcagc gtattgaacg
      601 tgaaaaacag cgacagacgc gatcgcgtgg catgggtggt atggagcgtt agttttgatg
      661 cgatgcagga tagggctaac gccgcaaagt gacgtcattt gcggcattca ccacgaaaaa
      721 attaatcatt cagatggtaa tcatgttttt ttaaaataga aaaagctgcg tttttctgct
      781 ttggtgacaa acccttagtt gagtatttat taccgctagt tctacgatat atgctgtaaa
      841 cactgcttaa aaaagtacgc tcccaaacag tcagaacctc attctttttt agtgaatcat
      901 gcagcattag cctgtctaca tttttaaaaa gtttatcttc agcattcatc atccgttacc
      961 ttcgtctgca ccgttcttcc attggtgtca taactgatct ttaactcaat tttctttctt
     1021 ctttcgtaaa ttgatggcca gtctccccgt gcaaaaagaa aggggttagc ttcaagttcc
     1081 ccacgccctg cccggaagaa tatttgttta tctacaagag ccgcaattat gtttttaaag
     1141 gtctgatatt caatatgaac cctgggctgt atggtttttt caaatctagt ttttgataca
     1201 gacacaaacc catcccatcc aatcagcatt gccaattgta aaagcacatc attttgagat
     1261 tttgataaac catgaatcat cgcaatgtct gcaatgtata gcttgacgta gggaggttcg
     1321 gctgggatgg aaaaggtctc gatcttagag tcttgtttta cctcaccgtt ttcatccatg
     1381 tatgtatgcc gttcaagcct agaaatcttt ttcgtattca ctccagcccc acgtttcgcc
     1441 atgatttagt actcgcattt aagtactaaa gagacataca gtcaagtcct ttgtatcaag
     1501 tacgattagt acttgaggtg agtacgatta gtccttgagg tgagtacgat tagtacttga
     1561 ggtgagtacg atcggtactt gaggtgagta cgattcttcg ctctagccct tggtacaagc
     1621 gggttcccat atctatatct atatattaag ggggaaaata agggctgaaa aacggcattt
     1681 ttagacactg aaaaactgcc tttttttagc aaggagtgta cgtatttcta tgtacgggga
     1741 gtggaaattc cgcttcctcg ctttggctgt tcgttcctcg tcgcgttgct cctgcggtac
     1801 gcctcagcca aagctgcggc gagcggttcc tgctctttca aagacgattt cattgcctga
     1861 tttcgtcaag ggcgagcgta gcgagtgcca tttacccttg acgacactcc ggataaaaca
     1921 ggcttggcag tggtgggggg tggcgcgaag cgacgccttc cacctgccag accgccgttg
     1981 attttgacct tctggcagct tgctgcccga acgattttgc gtagcaaagt ctagtgagta
     2041 cactatcatc ttgatagtgg cccgccggag gtatcgcaat gcgctccccc gcctggctga
     2101 atgaatgccg gaatcagggg ggaacatggc gaactacgcg ataatgcgct gcaaaaagct
     2161 gacgggaatg ggaagcgtgg ccagcgcgtt gcagcactgc taccgagagc gtgagacgcc
     2221 gaacgctaac gctgaacgga caccagaaaa ctactgctct gtgtccaaat caacggatca
     2281 ggcaatgggg cgtgtgcggg agctgctgcc ggaaaaacgc cgtaaagatg cggtactggc
     2341 cgttgaatac gttatgacag caagccctga gtggtggaaa gaggccacac cacggcagca
     2401 ggcggagttt ttcgcccgct ctgagcaatg gctggagaag aaatacggga aggatcgtgt
     2461 tgtggcggcg gtggttcacc gggacgaggc caccccgcac ctgtctgcgt tcgtggtgcc
     2521 attgacgcag gatggacggc tgtccgcgaa agagtttatt ggcgggcgg
//
