LOCUS       NZ_CP092378             1527 bp    DNA     linear   CON 08-APR-2024
DEFINITION  Enterococcus faecium strain V13-21-E11-012-001 plasmid
            pK21EFM001_1_7, complete sequence.
ACCESSION   NZ_CP092378
VERSION     NZ_CP092378.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23500290
            Assembly: GCF_021172105.3
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Lim,S.-K., Moon,D.-C., Kim,S.-J., Yeom,Y.-J., Kang,Y.-J., Kim,Y.-J.
            and Baek,H.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2022) Bacterial Disease Division, Animal and Plant
            Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do
            39660, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. V.70
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 32.94x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021172105.3-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 11:18:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,111
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,895
            CDSs (with protein)               :: 2,895
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 42 of 127
            Pseudo Genes (incomplete)         :: 80 of 127
            Pseudo Genes (internal stop)      :: 25 of 127
            Pseudo Genes (multiple problems)  :: 16 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092378.1.
            Bacteria and source DNA available from Animal and Plant Quarantine
            Agency Bacterial Disease Division.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="V13-21-E11-012-001"
                     /host="dog"
                     /db_xref="taxon:1352"
                     /plasmid="pK21EFM001_1_7"
                     /geo_loc_name="South Korea: Daegu"
                     /lat_lon="35.8303858 N 128.6299486 E"
                     /collection_date="2021-01-05"
     gene            55..141
                     /locus_tag="LRL10_RS15435"
                     /old_locus_tag="LRL10_16215"
     CDS             55..141
                     /locus_tag="LRL10_RS15435"
                     /old_locus_tag="LRL10_16215"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(490..699)
                     /locus_tag="LRL10_RS15440"
                     /old_locus_tag="LRL10_16220"
     CDS             complement(490..699)
                     /locus_tag="LRL10_RS15440"
                     /old_locus_tag="LRL10_16220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_240213323.1"
                     /translation="MIKRKRASISTKNGEFTACYEDMNNIFVDQEKRLTDKNMKKLTRF
                     YQNLPEKQQKEVQEKYEQVKKDVQ"
                     /name="hypothetical protein"
     gene            complement(671..949)
                     /locus_tag="LRL10_RS15445"
                     /old_locus_tag="LRL10_16225"
     CDS             complement(671..949)
                     /locus_tag="LRL10_RS15445"
                     /old_locus_tag="LRL10_16225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_240213325.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKSKH"
                     /name="hypothetical protein"
     gene            complement(1194..>1527)
                     /locus_tag="LRL10_RS15450"
                     /old_locus_tag="LRL10_16230"
                     /pseudo=""
     CDS             complement(1194..>1527)
                     /locus_tag="LRL10_RS15450"
                     /old_locus_tag="LRL10_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="frameshifted; incomplete; too short partial abutting
                     assembly gap; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
ORIGIN
        1 tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta tctaatgttt
       61 gaacggttca tttgccccct tcttgtagaa gtttgtgtct ctttattcag ttattggttg
      121 aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa aaagccccag
      181 agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc aggttcagtt
      241 taacatgatt gctaatcaaa ttcaattttg ttgttgctga tccaaccatt ccttgacact
      301 ttctagataa tttaatgccg tttctttttt cgttggattt tgtatgtttg ttgcttgctg
      361 aagcagtgtt tcataacgtt cgtatgtgac cgattgttgc cgttctttcg ttcttgttca
      421 ctggttgaaa aagggattgt atcgctaaat aagttacaac aagcttcttc tgtctgcgta
      481 tctttaaaat tattgcacgt ctttttttac ttgctcatat ttttcttgta cctctttttg
      541 ctgtttttct ggtaagtttt gataaaaacg agtgagtttt ttcatgtttt tatctgttaa
      601 acgtttctct tgatccacaa aaatattgtt catgtcctca taacaagcgg taaattctcc
      661 attttttgtg ctaatgcttg ctctttttct ttttatcaca tgctgcttca tacttttgag
      721 tgatatcccc taataaaatg cttcttgaaa cacgtttatt accctctaaa acgccgaata
      781 aaccacttaa aacgattgat aaggcaaata taaccccgac caataaaaat cgcttaaatc
      841 gaattttaga gccttttaat gaatatcttg tttgataagc cttatagcta agccaaataa
      901 ttcctaaaat actggcagaa atgagtagat agggtaaata gaatgccatg tctgttttat
      961 tctccccata ccaaccaaaa ggctaataat gttagtacta agcaacataa gggggcaaga
     1021 atatccatcg atcgttgaat taaagccttt ttttgttctt ggtttcgctg gtctaatagc
     1081 gcttggtgga gttttttgac ttgtgaatcc atcgtagttt gtatcctttc ttgggtataa
     1141 ggcgcaagaa ggcacgttgt ctctttattt cgttttcgat gtcttttaac gttttagagc
     1201 gataattctt gttggtggtc tgttcctcgt tcaacgtgtt gttcatggtt tcttcgagtc
     1261 ggtctaattt ccgattgttc aggcgtaaaa gttcgttgtt ctcttcgtac ttcttctgca
     1321 gtttcgcgaa tgctagctcg agctcttcgt agctcgttgg tttcgttgtt gattgtaggt
     1381 tcgttctcgt ctgtccagct catgtaaaat cgtctccttt tcgtaatcct ttcctagttt
     1441 gttggatcgt acttttttct ttttgtggac caattcgtaa gtaaaatttt ttccgcgatt
     1501 caagcaatga atgccgaatg ttttaag
//
