LOCUS       NZ_CP092343             3770 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KPN20 plasmid pKPN20-7, complete
            sequence.
ACCESSION   NZ_CP092343
VERSION     NZ_CP092343.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25953952
            Assembly: GCF_022354525.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Hou,B.L.
  TITLE     The Characterization of OXA-232 Carbapenemase-Producing ST15
            Klebsiella pneumoniae in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Hou,B.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2022) Department of Clinical Laboratory Medicine,
            The First Hospital of Jiaxing, 1882 Central South Road, Jiaxing,
            Zhejiang 314001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method :: CANU v. v1.8; Unicycler v. v0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 7129.6x
            Sequencing Technology :: Illumina NovaSeq; Nanopore Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022354525.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 04:18:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,268
            CDSs (with protein)               :: 5,268
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 57 of 172
            Pseudo Genes (incomplete)         :: 114 of 172
            Pseudo Genes (internal stop)      :: 26 of 172
            Pseudo Genes (multiple problems)  :: 22 of 172
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092343.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN20"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN20-7"
                     /geo_loc_name="China:Jiaxing"
                     /collection_date="2020-07-31"
     gene            complement(join(2858..3770,1..86))
                     /locus_tag="MG291_RS27735"
                     /old_locus_tag="MG291_27735"
     CDS             complement(join(2858..3770,1..86))
                     /locus_tag="MG291_RS27735"
                     /old_locus_tag="MG291_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="WP_001477600.1"
                     /translation="MGHGVTVLFLLLLLVHIIFDFYLQTEEMVREKAFDNGIGRALKAH
                     LFHAVMHGFFLCLSCFAFIYVLLGCTNLLNFMLFVLLFSLFTFFHFAIDVAKSITSLKV
                     KGFYCFAVFLFDQVLHILTIFFVIYYFFCDYGVYIYDFNFIKYVGGVLIFACSIAFLLK
                     PSSIIVYLFFKSSGIGDGSEKVKITKSKIAELLYIVLNKELGDSDVKDCKVAVNKYKKK
                     AEEIVIGISDGQADIDIRTISKVNNAGRFIGYLERLIIFLFLILGSVTAVVAVLAMKTA
                     LRFSDLKDDNDSGKAEYIMIGTFFSLIVTVIISVLARMYLIEIGIKINDFS"
                     /name="DUF3307 domain-containing protein"
     gene            complement(97..765)
                     /locus_tag="MG291_RS27740"
                     /old_locus_tag="MG291_27740"
     CDS             complement(97..765)
                     /locus_tag="MG291_RS27740"
                     /old_locus_tag="MG291_27740"
                     /inference="COORDINATES: protein motif:HMM:NF016205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059034.1"
                     /translation="MSDSGLMMGVITGDLYRSSTDYERGVSYIGLMEVMLEKIKKNNRL
                     RVSDVDFFRGDSFQITSKDPINLLSIAVYIRAYLRSYSEKNDGKGMYDARLAISIDKVN
                     KYRRDINSIFESAQIKSGKSLDLIENDEMMVFDSSLYDLKEIYSPSIILLDALLSMLSK
                     PQSEILREIVYDRDASISEIAERLGKSKQVVYKTIKRGAVDKIIKFLDAHLSLLGMRFF
                     "
                     /name="hypothetical protein"
     gene            complement(1153..1476)
                     /gene="mobA"
                     /locus_tag="MG291_RS27745"
                     /old_locus_tag="MG291_27745"
     CDS             complement(1153..1476)
                     /gene="mobA"
                     /locus_tag="MG291_RS27745"
                     /old_locus_tag="MG291_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_052522663.1"
                     /translation="MTKRSGSNTRRRAICRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1760..1954
                     /locus_tag="MG291_RS27750"
                     /old_locus_tag="MG291_27750"
     CDS             1760..1954
                     /locus_tag="MG291_RS27750"
                     /old_locus_tag="MG291_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041619.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2441..2590
                     /locus_tag="MG291_RS27865"
     CDS             2441..2590
                     /locus_tag="MG291_RS27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_162847635.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPGRHDKAPLAAAIDKSRCARDFR
                     ECLK"
                     /name="replication initiation protein"
     Typing          complement(1486..1722)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          1990..2510
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.687"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(1157..1476)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="1.44e-61"
ORIGIN
        1 accatctcct cagtttgcaa ataaaagtca aaaattatat gaaccaaaag caacaacaaa
       61 aatagaacag ttacaccgtg ccccataccc cctccattaa aaaaatctca ttccaagcag
      121 tgaaagatga gcatccaaaa actttattat cttatcaacc gctcctcttt ttatggtttt
      181 ataaacaact tgtttactct ttcccagtct ttctgctatt tcagaaatac ttgcatccct
      241 atcatataca atctccctta ggatttctga ttgaggtttg gacaacattg aaagcaatgc
      301 atcaagaaga ataatagatg gggaatatat ttcctttaaa tcataaagtg atgagtcgaa
      361 gaccatcatc tcatcatttt caatgagatc taaacttttc cctgacttta tctgagcaga
      421 ttcaaatatt gagtttatat cccttctata cttattaact ttatctatgg atatcgccaa
      481 gcgagcatca tacatccctt taccatcgtt tttctcacta tatgatctta aataagccct
      541 aatataaacg gcaatactta ataaattaat aggatctttg ctggttatct gaaatgaatc
      601 ccctcgaaaa aaatcaacat cactaactct taacctattg tttttcttta tcttttctaa
      661 cataacctcc atcaagccaa tgtaagaaac ccctcgttcg taatcggtac tcgagcggta
      721 caaatcacca gtgataaccc ccatcattaa ccctgaatca ctcatttcat cccccaaact
      781 aaaataattc ttgacagatt gcacaaccga aaaggaaaat agtcaaccaa aaaggttgaa
      841 aaacaaaaaa gtaacccatt ttggtttaca ccaataactt gatacttctt ggctctaagg
      901 cgctcaatat taaaattcag ttgtcaccct gcatttaaca taaagccata attgtacgca
      961 ccgctgaaac gcgctgaacg cgattctgac gcagacaccg caaaaagaga tgttttaccc
     1021 cgggaaaaca ggaaggatgc gtcaggatcg ttttcagctc gctgcatagc tatgcatgaa
     1081 agcgaatggt gatcactttg ggagcttacg gtgttcatac cgtctgtttt cgacagtttt
     1141 ctctccggga agctaatctg ccataagcct ggataacagg gcacggtgat actccgtaat
     1201 agcgatcagc acctccgcat actccctgtc cccgacacgc ttgccgtcga taaagagttt
     1261 tttctgtaac gcccccagtc tcataacttc tttcagtacc cgatcatcag tcagtgagtt
     1321 aactttctta ccgagagctg ccgcccgtaa aaaaccggaa acggtcttgc cgcattcagc
     1381 agcccttttt ctgatttcct ggtcttcttc tgccgttaga cgaacagggc gacagatagc
     1441 ccgcctgcgc gtattacttc cacttcgttt tgtcataaaa catggctccg tatctgacat
     1501 gggtgtcggg gcaaagccct gaccagggta attgtaatag cgtgcatgca tgcgcggtat
     1561 aacaattaca catcctgtcc tgtcagcaag ttcgaattac cgacaaagac acggcagttt
     1621 tttggcagtg acagtattgt caggcagaaa tacccgtcaa agcgtcagac ggcagaatac
     1681 cggcggtaaa acagtcgaaa agtggtacgc gatataatca cacaacgcag atgacagaca
     1741 gaacggagaa aacgaaaaga tgaacaagca gcagcaagcc gtactcaaca tggcaggatt
     1801 cataaaaagc cagagcctga cgctgctcga aaaactggac gcactcgatg ctgacgaaca
     1861 ggccgccatg tgtgagaagc tgcacgaact cgcggaagaa cttcagaaca gcatacagac
     1921 acgctttgaa gcggaaaatc gcaccgggat ataacgcatc cggacaggat gcagaacggc
     1981 atagaggagc gtaaggagaa aatgtcacat aaggcgctct tccgcttcct cgctcactga
     2041 ctcgctacgc tcggtcgttc gactgcggcg agcggtgtca gctcactcaa aagcggtaat
     2101 acggttatcc acagaatcag gggataaagc cggaaagaac atgtgagcaa aaagcaaagc
     2161 accggaagaa gccagcgccg cagacgtttt tccataggct ccgcccccct gacgagcatc
     2221 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggacttaaa gataccaggc
     2281 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2341 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
     2401 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2461 gcccgaccgc tgcgccttat ccggtaacta tcgacttgag tccaatccgg gaagacacga
     2521 caaagcgcca ctggcagcag ccattgataa gagtcgatgt gcgagagatt ttagagagtg
     2581 cttgaagtga tggccggact acagctacac taaaagaaca gttttggtgg ctcgtctcgc
     2641 acaagacagt tacctggtta agcagttccc caactgactt aaccttcgaa aaaccacctc
     2701 cccaggtggt tttttcgttt ccagtgcaag agattacgcg cagatataaa tgatctcaag
     2761 aagatccttc tcagatacga aaatgcatca tcattccatt gatatcattt ctcatcattg
     2821 caaccatcat cttcttatgt gagaaactga gctcaaacta actaaaatca ttaattttta
     2881 taccaatctc aatcaagtac attctggcta acacagatat tatcacggtt acaataagac
     2941 taaaaaatgt accaatcatt atatattcag ccttcccact atcattatca tcttttagat
     3001 cactaaatct cagagctgtt ttcattgcta acaccgcgac aaccgcagtg accgagccta
     3061 aaatcaaaaa caaaaaaatt atcaatcttt caaggtaacc tataaatcta ccagcattat
     3121 taactttgga aatagttctg atatcaatat cagcttgccc atcagaaata ccgattacaa
     3181 tttcctctgc ttttttcttg tatttattta ctgccacctt gcaatcttta acatcagaat
     3241 cacccagttc tttattcaat acaatataaa gcaattccgc aattttactt ttggttattt
     3301 taaccttctc tgaaccatca ccaatacctg atgatttaaa gaataagtaa actataatag
     3361 atgaaggctt tagtaaaaac gctatagaac aagcaaatat caacaccccg ccaacatatt
     3421 ttatgaaatt aaaatcatat atatatacgc cataatcaca aaagaaataa taaataacaa
     3481 aaaatatagt taaaatatgc aaaacttgat caaacaagaa aacagcaaaa caataaaaac
     3541 cctttacttt cagagaggta atcgactttg cgacatctat tgcaaaatga aaaaatgtaa
     3601 ataaagaaaa cagcaaaaca aatagcataa agtttaatag attggtacag ccaagcagta
     3661 catatatgaa agcgaagcag gatagacata gaaaaaaacc atgcatcacg gcatgaaata
     3721 agtgagcctt taaagcccta ccaatgccat tatcgaatgc cttttctcta
//
