LOCUS       NZ_CP092342             5640 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KPN20 plasmid pKPN20-6, complete
            sequence.
ACCESSION   NZ_CP092342
VERSION     NZ_CP092342.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25953952
            Assembly: GCF_022354525.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Hou,B.L.
  TITLE     The Characterization of OXA-232 Carbapenemase-Producing ST15
            Klebsiella pneumoniae in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Hou,B.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2022) Department of Clinical Laboratory Medicine,
            The First Hospital of Jiaxing, 1882 Central South Road, Jiaxing,
            Zhejiang 314001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method :: CANU v. v1.8; Unicycler v. v0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 7129.6x
            Sequencing Technology :: Illumina NovaSeq; Nanopore Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022354525.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 04:18:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,268
            CDSs (with protein)               :: 5,268
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 57 of 172
            Pseudo Genes (incomplete)         :: 114 of 172
            Pseudo Genes (internal stop)      :: 26 of 172
            Pseudo Genes (multiple problems)  :: 22 of 172
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092342.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN20"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN20-6"
                     /geo_loc_name="China:Jiaxing"
                     /collection_date="2020-07-31"
     gene            complement(46..531)
                     /locus_tag="MG291_RS27695"
                     /old_locus_tag="MG291_27695"
     CDS             complement(46..531)
                     /locus_tag="MG291_RS27695"
                     /old_locus_tag="MG291_27695"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139914218.1"
                     /translation="MSEILNLAKAFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMLRASVSTAMAWIFLCFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLESLKAKSGAGVAIYSDDKHRNTYMVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<578..1000)
                     /locus_tag="MG291_RS27700"
                     /old_locus_tag="MG291_27700"
                     /pseudo=""
     CDS             complement(<578..1000)
                     /locus_tag="MG291_RS27700"
                     /old_locus_tag="MG291_27700"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1209..1535)
                     /locus_tag="MG291_RS27705"
                     /old_locus_tag="MG291_27705"
     CDS             complement(1209..1535)
                     /locus_tag="MG291_RS27705"
                     /old_locus_tag="MG291_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_038885858.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPEVAPELLRQVSGIGNNLNQIARRLNQADSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1767..3542)
                     /locus_tag="MG291_RS27710"
                     /old_locus_tag="MG291_27710"
     CDS             complement(1767..3542)
                     /locus_tag="MG291_RS27710"
                     /old_locus_tag="MG291_27710"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="WP_124046799.1"
                     /translation="MRFDASPFNMNSILSVEKRYEIPRNQREFSWERIQLEELWADLLR
                     NIVMDDNGTIKLHEYFIGTIVLSGVDSDDVLEVVDGQQRLSVITMMLSIISRYFRKNKN
                     DTTADNIYKKYIVTINNTLDRNSLNSDGESVIQKLTRSVGGEYFKKIVQGKEESDAKAT
                     NDEEKKIRYAVTFFKRALRKKSLCSAIFKTNDDSFTKADYDLCLNAIYKMITNYLYIVR
                     IAVDRGDDAYDIFEVLNARGINLSSIDLIKNKVFQSCTTTFPEDEARRKWVHIYEQLGS
                     VGETMTDYIRCWWLSKYGYIGEDQLYRSFKREILNENSLLTADSFLEEIYNDVSLYMSM
                     VAPSTKYWNLSHQRPILDALANINTFSVTVPRPFILSLLRTRRDKSRLMKQSELIETLE
                     FLEKFHFKFNAVCRMRPSGIDSKYSVFAVRADRAVSKASLKNTIDETVDFLKRKAPSEK
                     VFKDCLMKNIEYKDDKLSQRKLIIYIFETLERLAVGTKELKYHMVSIEHIGSQSNFSPT
                     YVGKLGNLLPLCFDINRDCENMPLAGKLPEYARSRLELVKQFNTANAAKNTWEVSDAQT
                     RQEQITNALNAAFTL"
                     /name="DUF262 domain-containing protein"
     gene            complement(3532..4425)
                     /locus_tag="MG291_RS27715"
                     /old_locus_tag="MG291_27715"
     CDS             complement(3532..4425)
                     /locus_tag="MG291_RS27715"
                     /old_locus_tag="MG291_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_044816547.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRTGAAIDWNDRNAPAPTLTITNPDNGHAHLLYALRTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHSHWRIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERATAYNLHFSSPLD
                     DNEVTGIAKSIAKWTSKNFSEDSFREFVSRTHTSEIQSIRGSKSKGGGRPSIGEPWLEH
                     GISRATYFRKLKKNEI"
                     /name="replication initiation protein"
     gene            complement(4618..4902)
                     /gene="nadS"
                     /locus_tag="MG291_RS27720"
                     /old_locus_tag="MG291_27720"
     CDS             complement(4618..4902)
                     /gene="nadS"
                     /locus_tag="MG291_RS27720"
                     /old_locus_tag="MG291_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            complement(4904..5236)
                     /locus_tag="MG291_RS27725"
                     /old_locus_tag="MG291_27725"
     CDS             complement(4904..5236)
                     /locus_tag="MG291_RS27725"
                     /old_locus_tag="MG291_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_044816545.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAVLKSLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5392..5637)
                     /locus_tag="MG291_RS27730"
                     /old_locus_tag="MG291_27730"
     CDS             complement(5392..5637)
                     /locus_tag="MG291_RS27730"
                     /old_locus_tag="MG291_27730"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_306589344.1"
                     /translation="MTKTMTEMEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATS
                     QALTHSDRVCQHLSSQVDSLRVQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(3..1219)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.03"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cggtgttaaa aacgcgctgt gtcgtttcag ggcgctctga cggggttatt tcgccgtgta
       61 tttcactacg gtgttgtcgg tcgtcgatgt ataggtttcc acgccgtgcg cctgcttcgg
      121 cagcaccacc atgtaggtgt ttcggtgctt gtcgtcgctg tagatcgcca cgcccgcgcc
      181 gcttttcgct ttcaggctct ccagctgttg gctgtagctg tccatctcgc tcaggcgcga
      241 ggtgatgatc gtgccctgaa accagattat cccgcccatg atgccgatca gcagggcgaa
      301 gcagaggaat atccacgcca tcgccgttga gacagaggct ctgagcatgc ccgtcagtct
      361 gccgttctgc tcgcggatag cgttgctgat gctctgcgag cttgattgca gttccgcgct
      421 gatagacgtc tccagcctcg cgaactcgcg cttcacgctc tgctcggtat cctgcgcctg
      481 ctgcttcgat ttctcctcga aggccttcgc caaatttaaa atctcgctca taaatcattc
      541 ctttcagccg gatatttcgg ccgccgtccg gatcggcaat gctgatgctg cttttcgttt
      601 cccgcgctac ggtcaggccg tagctttcca gctgcgtcac cacgtcctgc cggctgcgga
      661 tcaccccctg ctgcatcagg ttcatcaggc cggcggtgat tttctctgcc gcctgctgtt
      721 tgtcgcgggg caaatcgctg gcctgcgtca gcggacggcg gtaggtcggg tcattcgggt
      781 cgcgcagccc cagccggtcg ttggtcaggg tctgccaggc gttgacgcgg ggacggtcgg
      841 cgcggtcaaa gtagggttgc aggcgttttc cactctgcaa ttcgatgttc gggatgacaa
      901 aattcagctc aagccgtccc ttgtcacggt gctccaccca caggcaggcg tactggtcgc
      961 gatcaaggcc ggtcagcagc gtgtgctccc attcgtccat caggcgcgat ttctcagcgt
     1021 ctgcaatgtc cggctcctga aatgacagca cgccggaagt ataagcgcgg gcaaagtcgc
     1081 agccgtcgat cagctcgcgc acatgttcag ggttgccgcg cagaacgctg gcctgttcac
     1141 gctgccggtc tttacccagc aggtaatcca ccggcccgct gcccgaaccc gcaccccgtg
     1201 agtgaatttt aacgatcacg gttctgctcc agcaggtcgc caagctgccg gtcaaggctg
     1261 gtcagcacga cgagcagcga ggcacgttca gaaggcgtca gggaatcagc ctggttcagg
     1321 cgacgggcga tctggttcaa gttattgcca atcccgctga cctgccgcag cagttcgggc
     1381 gccacctcag gcacacggcg cttgcgggag gtgtgttcac ccagaccaag ctgacgcagc
     1441 cactccgcaa ggtgagtgcg gtcgcagcga tcaagcagtc gctgatgctc ggtttcggtg
     1501 agccggattt tgatctcttt cgttcgcttt tccataggct ccgctgagaa aaaattcgca
     1561 ccaccgtgag aatttttcgc agtggatgct gggggtttcg gggggcggcg agccccctga
     1621 accagtcacg gcgacacctc gtaagagggg acgctgtgtg tactggctta tagtatgaag
     1681 tagtatgtca gattctatgc tatccgcaaa aaaacagccc tctgctaaag agggctgtta
     1741 tctgatatag gttgtttagt ctcacatcag agcgtgaagg cagcgttgag ggcgtttgtt
     1801 atctgttctt gcctcgtttg ggcatcactt acttcccaag tatttttagc tgcgttagca
     1861 gtgttaaatt gcttcactaa ctctaatcgt gatcgagcat attctggcaa tttcccagct
     1921 aatggcatat tttcacagtc tctatttata tcgaaacaaa gcggcagaag gttacccagc
     1981 ttaccaacat aagttggact gaaattcgac tgagagccta tatgttcaat cgaaaccata
     2041 tgatatttaa gctctttagt tcctactgct agtcgctcta gtgtttcaaa tatgtagata
     2101 atgagttttc tttgagatag tttatcgtcc ttatactcga tatttttcat taagcagtct
     2161 ttaaacacct tttcgcttgg tgcctttctt ttcaggaaat caactgtctc atcgattgtg
     2221 tttttcaatg aagccttgct cacagctcta tcagctctga ctgcaaaaac tgaatatttt
     2281 gagtcaatac ctgaaggtct cattcgacat acagcattaa atttaaaatg gaacttctca
     2341 aggaactcta gcgtctcaat aagctcagac tgtttcataa gccttgattt atctcttctt
     2401 gttctgagaa gagataaaat gaaaggtctt ggaacagtaa cgctaaatgt gttgatattt
     2461 gctaaggcgt ctaatatagg tctctggtga gacaggttcc agtattttgt agatggagct
     2521 accatactca tataaagaga tacatcatta tatatttctt ctaaaaatga atcagctgtt
     2581 aacaagctat tttcatttaa aatttctctt ttgaatgatc gatagagctg atcttcacca
     2641 atatatccat atttagatag ccaccaacaa cggatgtaat cggtcatggt ctcgccaact
     2701 gaacctagct gttcgtatat gtgaacccat ttccttctgg cttcatcttc tgggaaagtt
     2761 gtagtgcaag actggaaaac cttattcttt ataaggtcta ttgaactgag gttaatacct
     2821 ctggcattta gaacttcaaa tatgtcatat gcatcgtcgc ctcgatcaac cgctatcctg
     2881 actatgtaca gatagttggt tatcatttta tagatagcat taagacataa gtcataatca
     2941 gccttcgtaa aactatcatc atttgtttta aatatggcgc tacataatga ttttttgcga
     3001 agagcccttt taaagaacgt aacagcatat cttattttct tttcttcatc attcgttgct
     3061 ttggcgtcag actcttcctt gccttgaact atttttttaa aatactcacc tccaacgctc
     3121 cttgttagct tttgaataac agactcacca tcagaattta aactatttct atccagagtg
     3181 ttatttattg ttacgatata ttttttatat atattgtccg cagttgtgtc atttttgttc
     3241 tttctaaaat accttgatat tatagaaagc atcatggtta ttacagacaa tcgttgctgt
     3301 ccatccacaa cttctaaaac atcatcacta tcaacacctg atagcacaat ggttccaata
     3361 aagtactcgt gtaattttat agtgccgtta tcatccatca ctatatttct tagaagatca
     3421 gcccaaagct cctcaagctg aattcgctcc cagctaaact ctcgctgatt tcttgggatc
     3481 tcatatcttt tttctacaga aagaatggag ttcatattaa atggtgacgc atcaaatctc
     3541 attttttttt agtttcctga agtatgtagc tctgctgatg ccatgttcta accacggctc
     3601 ccctatagaa ggtcttccgc ctcccttact tttactaccc cgtattgact ggatctcaga
     3661 ggtatgtgtc cgagaaacaa actccctgaa tgaatcttcg gaaaaatttt tacttgtcca
     3721 tttagctatg cttttggcaa ttccagttac ttcgttatcg tcaagaggcg aagaaaaatg
     3781 aaggttatag gctgtagcac gttcataaca ggcttgcagc cactggtcat actcaggcca
     3841 gccctgacgg atagcgcggt atgcccactt gcgggttttg tcgaacagcg tgcagttacg
     3901 gcctaaacca tagtcggcaa cgatttcttt gtcgttcgcc gcgttcaggt cgagaaagtc
     3961 agccagccag tccagcgtgt agagttccgg ctgccatacg gcaattctcc agtggctatg
     4021 gttcggattt ttgcagatta gccccgaata ccccgcgtca gcctctaact ttttacgtag
     4081 cgcgttctcc acagcggcag cgtagcgcag cggcttcatt tttccgtccg gcgcggtacg
     4141 tatcgaggtt ctgagggcgt acagcagatg cgcgtgtccg ttgtcggggt tggtgatggt
     4201 cagcgtgggc gcaggcgcgt tccggtcgtt ccagtcgatg gccgcaccgg tgcgatcgac
     4261 gtcgaatccc agccagaaca tggcgtgcgg ctggttgaac tggatgtatt tcgccagaat
     4321 ggcgcgttcc ttaccggcaa tgcgtacacc aaagtgtaaa tcatccgaga agtacggctt
     4381 gtgcggtaag cggtcgttga aaagttcgag tactgcgtta ttcaaagcgc cctgcactta
     4441 tggcgggctg ttgtctggca cggttcatgt gctaatatcc tttcaaggtt tcgacctggc
     4501 cctggttatc atgtccgctc gccaaagtag agcatgattt ccggggcttt gttttttctg
     4561 ttgccgttaa attacacgcg aacggcctcg cctgtcgaat ccttctgtca caacggctta
     4621 gtgcgccgcc agctcctgga agaatttcgg gttcgcctgg atggttgcca gcaccttcgc
     4681 cgccagtccg gtcggattac ggcgtccggt ttcccagctc ttgatagtgt cgaggctggt
     4741 ccccagcact ttcgccatct cgctctgaga tacgttcagc tgtgccctga tggctttcac
     4801 gtctgccagc tcgtaacggg tgacgcgggc aggctctctg acgccgtttt tgatttcaac
     4861 ggcttcttcc agcgatgctt tcagctcgtc aaaaaaactc atgtcacacc tcgtctttca
     4921 gttgttgggt cagcgacttc agcacggctt tctccgcgtc cgtcaggctg tctttggtgc
     4981 ttttcgggta ggccatcacc agataaatca cctcggcggt ggccagaaag taaataaccc
     5041 tcgcactgcc gctttttccc tggttgcccg tcgccattct gattttgcgc agcccaccgg
     5101 ttttctggat gagatcgcct ttatccggtg actcaatcag cgtcttttgc agctccctga
     5161 gttcttcatc cgtggcgatc tgttttatct gacgggtaaa catcggggtc tcgataaact
     5221 caatcgcgtg actcacggct gtctcctgtg ttcaatggcg tacatagtac acttaatttt
     5281 aatttcagaa aagcggtcac atcgaggggc cgtaatagcg ggtcggcttt ttctgcggct
     5341 ccggctgctg ctcgcgcagc ttctccgcac gggcgcggtc gtgctgtgca atcagcgcat
     5401 caagcggctg acgttcctgc ttaagctctc gacctgcacg cgcaagctgt ccacctgact
     5461 gctcaagtgc tgacacaccc tgtcgctgtg cgtcagcgcc tgtgaggtag cctcgaacat
     5521 gtgctgcaag ctgctgtagc tgtcctgcca cgcctgctgc tgttgctcgt agcttgtctg
     5581 taagctctct aaggcgctca gaagctgttt ttccatctcg gtcatggttt tggtcatcct
//
