LOCUS       NZ_CP092340             8069 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KPN20 plasmid pKPN20-4, complete
            sequence.
ACCESSION   NZ_CP092340
VERSION     NZ_CP092340.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25953952
            Assembly: GCF_022354525.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Hou,B.L.
  TITLE     The Characterization of OXA-232 Carbapenemase-Producing ST15
            Klebsiella pneumoniae in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Hou,B.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2022) Department of Clinical Laboratory Medicine,
            The First Hospital of Jiaxing, 1882 Central South Road, Jiaxing,
            Zhejiang 314001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method :: CANU v. v1.8; Unicycler v. v0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 7129.6x
            Sequencing Technology :: Illumina NovaSeq; Nanopore Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022354525.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 04:18:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,268
            CDSs (with protein)               :: 5,268
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 57 of 172
            Pseudo Genes (incomplete)         :: 114 of 172
            Pseudo Genes (internal stop)      :: 26 of 172
            Pseudo Genes (multiple problems)  :: 22 of 172
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092340.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN20"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN20-4"
                     /geo_loc_name="China:Jiaxing"
                     /collection_date="2020-07-31"
     gene            complement(join(7810..8069,1..187))
                     /locus_tag="MG291_RS27600"
                     /old_locus_tag="MG291_27600"
     CDS             complement(join(7810..8069,1..187))
                     /locus_tag="MG291_RS27600"
                     /old_locus_tag="MG291_27600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(974..1384)
                     /locus_tag="MG291_RS27605"
                     /old_locus_tag="MG291_27605"
     CDS             complement(974..1384)
                     /locus_tag="MG291_RS27605"
                     /old_locus_tag="MG291_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1384..1575)
                     /locus_tag="MG291_RS27610"
                     /old_locus_tag="MG291_27610"
     CDS             complement(1384..1575)
                     /locus_tag="MG291_RS27610"
                     /old_locus_tag="MG291_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1991..2104
                     /locus_tag="MG291_RS27615"
                     /old_locus_tag="MG291_27615"
     CDS             1991..2104
                     /locus_tag="MG291_RS27615"
                     /old_locus_tag="MG291_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(2311..2787)
                     /locus_tag="MG291_RS27620"
                     /old_locus_tag="MG291_27620"
     CDS             complement(2311..2787)
                     /locus_tag="MG291_RS27620"
                     /old_locus_tag="MG291_27620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(2787..3431)
                     /locus_tag="MG291_RS27625"
                     /old_locus_tag="MG291_27625"
     CDS             complement(2787..3431)
                     /locus_tag="MG291_RS27625"
                     /old_locus_tag="MG291_27625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            complement(3433..3657)
                     /locus_tag="MG291_RS27630"
                     /old_locus_tag="MG291_27630"
     CDS             complement(3433..3657)
                     /locus_tag="MG291_RS27630"
                     /old_locus_tag="MG291_27630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            complement(4521..4943)
                     /locus_tag="MG291_RS27635"
                     /old_locus_tag="MG291_27635"
     CDS             complement(4521..4943)
                     /locus_tag="MG291_RS27635"
                     /old_locus_tag="MG291_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4943..5134)
                     /locus_tag="MG291_RS27640"
                     /old_locus_tag="MG291_27640"
     CDS             complement(4943..5134)
                     /locus_tag="MG291_RS27640"
                     /old_locus_tag="MG291_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_117083186.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5963..6319)
                     /locus_tag="MG291_RS27645"
                     /old_locus_tag="MG291_27645"
     CDS             complement(5963..6319)
                     /locus_tag="MG291_RS27645"
                     /old_locus_tag="MG291_27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(6523..6843)
                     /locus_tag="MG291_RS27650"
                     /old_locus_tag="MG291_27650"
     CDS             complement(6523..6843)
                     /locus_tag="MG291_RS27650"
                     /old_locus_tag="MG291_27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7026..7268
                     /locus_tag="MG291_RS27655"
                     /old_locus_tag="MG291_27655"
     CDS             7026..7268
                     /locus_tag="MG291_RS27655"
                     /old_locus_tag="MG291_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     Typing          1697..2104
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="100.0"
                     /evalue="1.26e-177"
     Typing          5254..5663
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtctggtgt gtgtagttcg ttcccatccg catcgagatt aataacttcg cctcgctcac
       61 caacagtaac aaagctctct acgtgagaca gatcatcaac aaccatcaca ttccttttca
      121 ttcttaaacc agtcactttt gcacctgcac cgatcttcat aaaactaaag cctgatttat
      181 cgttcactca catcacctca taagataaaa atgttaataa cgttttcacg agcaattatt
      241 taccataacg cctcattacg cgcaccgctc aaacgctcta gaaacgattc tggggcgtaa
      301 tccattaaat cctacgtctt accccctgaa aacgcctcaa aatcgctcca gggcgtttta
      361 cgggcatgaa ttgttatgca ccagactgac tggtggcgga tttcagcctg aattcaggcc
      421 agacaactca aaacccgcac caaaccagac tgaaaacggt gaacggggac gcaatttatt
      481 gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaacgg ttttcagcac gttcaggaat
      541 cgggttcgtc gacggccggc aatcacgtta tgcaagtcgc cggacgactc accacacgat
      601 tcaccggcga ttcgactcgt gacttgactc tagagtcgcc aaggtcaaaa aaacaccaaa
      661 atgacgattc gactctagag tcaagtcgtc ataagttact gatagtctta agtgagtcgt
      721 cgatgactct atgacgagtc gtcggcaagt cgtacacgaa tcgccgacga cttggattca
      781 ccttacagaa agtaatactg cgtaatgtaa taacccacaa ctaactgata ttaatgaatt
      841 atcttcaaaa ttttaatttt gcgtggggtg tgggtatttt tagtggtgag gagcgttcag
      901 agatctggcg atttttggga aggttcagga actccccggg atggccggag aggaaaacgg
      961 aagcatgacg gagtcaggat tttttccaga atttccacca cggggaatcg ggtggtgaag
     1021 aaggttcgtg cttgtgctcc agaagctgca tagcctgacg aagggaatca atatgctcat
     1081 ctcgagcctt aacagcccct ttaagaccat cgttttcagc ttctagtaaa gcaattctat
     1141 gttgtaaatc atgtaaacca acgctgttaa cacctgtaga tcctgcgtca ctttttacag
     1201 tatcgatgac accatcaaga gaaccaaaaa ctctaataag ctctgaagta tcaataactt
     1261 taatgatctt cccgtgcttt acaacagagg tgtaacttaa cttcccactg ttaatcatct
     1321 tgtaaattgt ggggcgacta accttaacaa gccgtgccgc ttcactaata gacactctgg
     1381 ccatcagtta ccatcctgta attcatccag tctcgaggac agtgtacgga aaagatgctc
     1441 cgcatcatcg tggagtttct cacaaaggtc ggcctctaca tcaagatcaa gctcgttaag
     1501 cttctccagc aggagcagag actgattctg gatgaattta gccatgttca gcgctgtttt
     1561 ctgttgtttc gtcataagag gcctcagaac gtcagtgctc agtgtaggga tgttctcttt
     1621 tccgcttcct cgctcactca gtcgctacgc tcctgccgtt cggctgcggc gagcggcagg
     1681 agcatgcgta taaaagctcg agaaaaccga gcgatcgcgt gatggggaga aggagaggcc
     1741 gcacagcggc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     1801 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggt
     1861 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     1921 tggccgcgtt tatctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     1981 gctggactgt atgcacgaac cccccgttca gtccgactgc tgcgcctttt ccggtaacta
     2041 tcaacttgag tccaacccgg aaagacacga cgaagcgcca ctggcagcag ccactggtaa
     2101 ctgatttaga ggagttagtc ttgaagttat gtgccagtta aggctaaact gaaagaacga
     2161 ttttggttac tgcgctcctc taactagcca gttaccttgg ttcatagagc cgcaaactct
     2221 tagaacctaa gaaaaaccgc cccgcaaagg cggtttttct ttttcaagaa gagtactagg
     2281 tcacacttga tgacgtcaag acggttttcg ctaaagagga acaaccacgc caccttcatt
     2341 tctttgaaaa aaagaggata attccaaaat agttgtagtc acctcaatca aagtaaattc
     2401 atcattaata tcaaataaat accacaaacc caagtgaggg taaccttcgt cctttatatc
     2461 aatacattta tctggtttgt taacttgctc gatcacttcc gctgcatcaa atgagagata
     2521 tatggcaggt ttggttttcc ctacagggat tcttttatgt acatccctta ccattccttc
     2581 ataataatct ttatttgaat gataaataga cacataatcc tctgaaattt cctccctaag
     2641 ataaaaacac ttaatagttg tagcgccaaa ctcatcttta tgatcttcca taacataacg
     2701 ccataaatat gaattatttg ctctgcccgt aaatgcatta atacaaggga ttgatttgag
     2761 gttatttaaa gttgataaca cagtcatcac cactccatca ttttaaataa tgatttgata
     2821 gcaatggagt tatcaaggct atcgccaata tatgccctac ctgtaacctt aaccactcca
     2881 gaaacttttc taacaaagga aaaaataatc tcgttatttg ttttcactat taaatttagc
     2941 gtgcctttgc ttttcttagt ttgtttatat ataagcccaa aacactctga ttttttatca
     3001 aagtaaatct gagcgtcttt ttttgcagca agttcagggg catacctaat caactgaaca
     3061 atagcctgca aagatggttc taggtcacca gtgcagaact cgtattctct attataaaca
     3121 gaaatggcac ttcttatttt tttaaaaaac aataagaatt cacctgataa ttctaatgat
     3181 tcgtaatgtt ttaaaattga ctcataaccg tcaccagagc aatctaatgt tttaacatca
     3241 tcattccaag actcccaaac agaattctca acaacaacaa atgctcgacc caaaacttta
     3301 aacaaatccg gatctcttaa atcatttagc tctaacttat tataatcaga aacatcataa
     3361 cgaacaccga gatcaatgct atcattagta atataataat cttcactatc gcagtggtta
     3421 atgtatttca tactaatcac ctgactcaac gaatttattc aatccatcga taaggttctt
     3481 tgcatgtttt ttggttagag ctatggaata aaaaaccctt cctgattctt tttcaggaaa
     3541 gtccactaac tctaaaaaac aaatatcttc agctatccca actcttagag ccatcgggaa
     3601 tacaggttca ttttttcttg gtgttacttt tctttctaaa gaatctttat tgctcattat
     3661 aataccccga tagaaataaa tttaagttta gaacataaaa aataatacca taaaacaaca
     3721 tctttcatcc acaatgctca tttaacatta cattggtcac tttatggaat tttgtggcca
     3781 tgatttaaca taacaccaaa attgtacgca ccgctaaaac ggcctcaggg cgtttctgac
     3841 gggaaaccgg taaaataggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct
     3901 gtttttacgg catgcatgag tatgcacgca agtgcatagc gcgaaaattc acgccattca
     3961 gccagggtaa gtcctgtttc cggcgttctt cccatccgaa aacggtgaac ggggacgcaa
     4021 tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aaccggtttt cagcacgttc
     4081 aggaatcggg ttcgtcgacg gccggcaatc acgttatggc aagtcgccgg acgactcacc
     4141 acacgattca ccggcgactc ttcactagag tcgtacccag agtcgtcacg tctaaaaaac
     4201 aaccaaacgg ggagtcgggt ctagagtcgg gtcgttgtta acttattgtt tttatagtcc
     4261 tgagtcgtta ccgactctat gacgactcgc cggggactct atgacgagtc gtcatagagt
     4321 cggatctgta taaataaaat taaaaaaacg aaatttatag aacgtaaact actgatataa
     4381 aatacaaatc tgcaaaattt taattttgcg tggggtgtgg atatttttag tggtgaggag
     4441 cgttcagaga tctggcgatt tttgggaagg ttcaggaact ccccggaatg gccggagagg
     4501 aaaactgaag catgacgggg tcaggatttc ttccagaatt tccaccacgg agaatgaacc
     4561 tgtggagagg gttcgagctt gtgttccaga agctgcatag cctggcgaag agaatcgata
     4621 tgttcatcac gagctttaac agccccttta agaccatcgt tttcagcttc aagcaaagca
     4681 attttatgct gtaaatcatg taaaccagag atgttaaccc ctgtagcgcc tgcgtcacct
     4741 tttacatcat gttttacagt gtcgccccca ccataaagcg agccaaaaac cctaataagc
     4801 tctgatgtat caacaacttt agtatttttc ccatgcttta caacagaggt gtaacttagt
     4861 ttcccactgt taatcatctt gtaaattgtg gggcgactaa ccttaacaag ccgagccgct
     4921 tcactaatag acactctggc catcagtttc catcctgtaa ttcatccagt cccgaagaca
     4981 gtgtacggaa aagatgctcc gcatcatcgt ggagtttctc acaaaggtcg gcctctgcat
     5041 caagatcaag ctcgttaagc ttctccagca ggagcagaga ctgattctgg atgaatttag
     5101 ccatgttcag cgctgttttc tgttgtttcg tcataagaag cctcagagcg tcagtgctca
     5161 gtgtaaaacc agtctctttt ccgcttcctc gctcactcag tcgctacgct cctgccgttc
     5221 ggctgcggcg agcggtagga ctatgcgtat aaatgctcga gaaaaccgag cggacgcgtg
     5281 atgggcagaa ggaaaggccg cttagcggcc gtttttccat aggctccgcc cccctgacaa
     5341 gcatcataga aattgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     5401 tcaggcgttt ccccctgatg gctccctcgt gcgctctcct gttcctgcca ttcggtttac
     5461 cggtgtcatt ccgctgttat ggccgcgttt atctcattcc acgccagaca ctcagttccg
     5521 ggaaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct
     5581 gcgtcttatc cggtaactat cgtcttgagt ccaacccggt aagacatgca aaagcgccac
     5641 tggcagcagc cactggtaac tgatttagag gagttagtct tgaagtcatg cgcctgttaa
     5701 ggctaaactg aaagaacaag ttttggtgac tgcgctcctc caagccagtt accttggttc
     5761 aatcacctgc aagtgtttga accttcggaa aaaccgccct gcaaggcggt ttttccgttt
     5821 acagagcaag agattacgcg taccgaaacc ggatctcaag tgctcgtaaa agaaaatgat
     5881 gatgaccttt agcggttaca aaaaaagaag gcacactatt acagaggcct tcttctagtt
     5941 tggttaaatt tttttaaaaa aatcatgcga ccgcatgttt tttttccaaa gattcagcat
     6001 tgtgcttcct ttctataatt tcatgagcgg gagtgactct ttctatttct tcctgaaggc
     6061 gtgggctgct cgtaacctcc cacaaatcaa cagactgctg tatatttaac cagaacttag
     6121 cggtagtatc gaatgcttta gctaaccgaa acgccatatc ggtagacagt ttcctattat
     6181 tgttaaccaa agcacttgca gaatttctat gcacgtttaa catttcagcc aagtcattga
     6241 tttttaaacc acttggctcg agatattcat aaagaataat atctcctaca gtcgatggtt
     6301 ttctggttgc ttgggacatg ctttactcct gaattggtcg cttctctctg ttagatgagg
     6361 aagataagta tttcttctgt taccagctgt atcgattagt aattatctta aatatagtta
     6421 cgtcgcgtta ctttattaat cattatttgt gatgacgttt taacgtaggt gaggaaatgc
     6481 ctcacctact tgttttgtaa gataacagtt ccggtctgtt acctatcggt aatcatgtgg
     6541 atcgagatac aaatcctcga ctccgttctc accccactta aatatcaagc ggtacttatc
     6601 attcaccctt atagatgaat accctgtcaa tggagggttg agattttcaa atcggttccc
     6661 cgggggagac cgtaaatctc caattttatc agcagcattc agcatatcca gcttgcgacc
     6721 taatgaaaca ctaatcgtag ctggaatttt tcgattggct ttaccgtatt tataaaactg
     6781 gtatagccaa tcgtctcgga actgaatgcg gcaatcatca tcactatcat gattttgctg
     6841 catttttctt ttacctcaaa tgttgccgca acttggcaag ttctagatca ccttttacca
     6901 ccaccatagt acaacaaacg ccagaaatgc acaatagcac tgtgcacttg tgcaaaaaac
     6961 cataatgata taaaatgcac aaaagcacag tgctactgtg caaaaaaaga atagttggag
     7021 gtcatatggc gcttgttcgc tcaggaaata aaaccaagat ttttgaagca gatccgaaat
     7081 ttaacccagt ttttgataca caacacaagc ctggcgatac gtgcacgtat gaaggaattt
     7141 acaaatgcac aggttgcggt aaagaagtaa ctatcgcagg agggaaaaca cttccaccac
     7201 aaaatcatca tcaacatagt cccggacagg gggacatccg ctggcaacta tttgtttatg
     7261 cagagtgagg tatgaaaatg gcagaccgta gaccacttaa cccgcatgtc gtcgtcccta
     7321 aaccaggagg cggaactcag ccacgagatc gtaataataa tggaagagtc cgagagaaac
     7381 gttcagatgc gggaaaatca agaaaataag aaaagccgcc aaataggggt cagtttggat
     7441 atcggaaact tttgtacgtt aagcgcatta tttaaccaat attgatgcgt aagggattga
     7501 ttttacctca gctacgatcg cttttaagtc accgctcttt ctgtatcgca tttaggttat
     7561 ccgattaagg aaaattcacg ggaaacggcg tatagcgcgg gagtacatca ttgcgagagg
     7621 taagcctgtt caaaacacag gcttaccgta caataattct ctatatccaa actgacccca
     7681 agaagtctga cactaacccc acacaaatcg aatccgttgc aagatgagac acacaagaaa
     7741 agagctagac gaaggtcgct ccgctcccca tggtttttgt atacccacca tgggggcata
     7801 ggtagtttat taactaactc caagataatg cgtgatcgca gccgtgagta caccacagcc
     7861 cacttgttta gataaatcta aaaccccttg taggattctc ttagtgttcc actcaccttc
     7921 tgcatccatt ggttgagata gtgcagcact caactggttc ttaatctttt gcgaagttat
     7981 atcgttatct attgagttga tttcctcctt ggctttctcc aaggaagcct ggagttcacc
     8041 gcgttctatt tttaactttt cacgataac
//
