LOCUS       NZ_CP092339            12435 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Klebsiella pneumoniae strain KPN20 plasmid pKPN20-3, complete
            sequence.
ACCESSION   NZ_CP092339
VERSION     NZ_CP092339.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25953952
            Assembly: GCF_022354525.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Hou,B.L.
  TITLE     The Characterization of OXA-232 Carbapenemase-Producing ST15
            Klebsiella pneumoniae in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12435)
  AUTHORS   Hou,B.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2022) Department of Clinical Laboratory Medicine,
            The First Hospital of Jiaxing, 1882 Central South Road, Jiaxing,
            Zhejiang 314001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method :: CANU v. v1.8; Unicycler v. v0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 7129.6x
            Sequencing Technology :: Illumina NovaSeq; Nanopore Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022354525.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 04:18:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,268
            CDSs (with protein)               :: 5,268
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 57 of 172
            Pseudo Genes (incomplete)         :: 114 of 172
            Pseudo Genes (internal stop)      :: 26 of 172
            Pseudo Genes (multiple problems)  :: 22 of 172
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092339.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN20"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN20-3"
                     /geo_loc_name="China:Jiaxing"
                     /collection_date="2020-07-31"
     gene            complement(533..2722)
                     /locus_tag="MG291_RS27540"
                     /old_locus_tag="MG291_27540"
     CDS             complement(533..2722)
                     /locus_tag="MG291_RS27540"
                     /old_locus_tag="MG291_27540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            complement(2719..3804)
                     /locus_tag="MG291_RS27545"
                     /old_locus_tag="MG291_27545"
     CDS             complement(2719..3804)
                     /locus_tag="MG291_RS27545"
                     /old_locus_tag="MG291_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            4181..4444
                     /locus_tag="MG291_RS27550"
                     /old_locus_tag="MG291_27550"
     CDS             4181..4444
                     /locus_tag="MG291_RS27550"
                     /old_locus_tag="MG291_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            4434..4733
                     /locus_tag="MG291_RS27555"
                     /old_locus_tag="MG291_27555"
     CDS             4434..4733
                     /locus_tag="MG291_RS27555"
                     /old_locus_tag="MG291_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4795..5172)
                     /locus_tag="MG291_RS27560"
                     /old_locus_tag="MG291_27560"
     CDS             complement(4795..5172)
                     /locus_tag="MG291_RS27560"
                     /old_locus_tag="MG291_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(5244..6815)
                     /locus_tag="MG291_RS27565"
                     /old_locus_tag="MG291_27565"
     CDS             complement(5244..6815)
                     /locus_tag="MG291_RS27565"
                     /old_locus_tag="MG291_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="WP_029392005.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEIN
                     RLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPSAL
                     RQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIETQR
                     PKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQGVEL
                     SRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYV
                     RDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAA
                     CMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKARAAPLMQ
                     SLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAENALRGVAVG
                     RKNWMFAGSNSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWK
                     VDLSSQ"
                     /name="IS66-like element ISCro1 family transposase"
     gene            complement(6835..7182)
                     /gene="tnpB"
                     /locus_tag="MG291_RS27570"
                     /old_locus_tag="MG291_27570"
     CDS             complement(6835..7182)
                     /gene="tnpB"
                     /locus_tag="MG291_RS27570"
                     /old_locus_tag="MG291_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_000624622.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLFI
                     FRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINWKH
                     PKRTERAGIRI"
                     /name="tnpB"
     gene            complement(7182..7859)
                     /gene="tnpA"
                     /locus_tag="MG291_RS27575"
                     /old_locus_tag="MG291_27575"
     CDS             complement(7182..7859)
                     /gene="tnpA"
                     /locus_tag="MG291_RS27575"
                     /old_locus_tag="MG291_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="WP_001339397.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKSTV
                     CSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPRRP
                     NFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPALLP
                     VTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIREIKG
                     SSH"
                     /name="tnpA"
     gene            7948..8127
                     /locus_tag="MG291_RS27580"
                     /old_locus_tag="MG291_27580"
     CDS             7948..8127
                     /locus_tag="MG291_RS27580"
                     /old_locus_tag="MG291_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8155..8685)
                     /locus_tag="MG291_RS27585"
                     /old_locus_tag="MG291_27585"
     CDS             complement(8155..8685)
                     /locus_tag="MG291_RS27585"
                     /old_locus_tag="MG291_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8692..9423)
                     /gene="mobC"
                     /locus_tag="MG291_RS27590"
                     /old_locus_tag="MG291_27590"
     CDS             complement(8692..9423)
                     /gene="mobC"
                     /locus_tag="MG291_RS27590"
                     /old_locus_tag="MG291_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(9423..11384)
                     /locus_tag="MG291_RS27595"
                     /old_locus_tag="MG291_27595"
     CDS             complement(9423..11384)
                     /locus_tag="MG291_RS27595"
                     /old_locus_tag="MG291_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     Typing          41..170
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.893"
                     /coverage="100.0"
                     /evalue="4.03e-48"
     Typing          12185..12435
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.841"
                     /coverage="100.0"
                     /evalue="2.61e-106"
     Typing          complement(8696..9423)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.88e-144"
ORIGIN
        1 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcagctcggt gtaggtcgtt
       61 cgctccaagc tgggctgtgt gcacgaactc cccgttcagc ccgactgctg cgccttatcc
      121 ggtaactgtt cacttgagtc caacccggaa agacacgcaa aaacgccact ggcagcagcc
      181 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
      241 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctcgtctc gcgaaagcca
      301 gttacccggt taagcagttc cccaactgac ttaaccttcg acaaaccacc tccccaggtg
      361 gttttttcgt ttacagggca aaagattacg cgcaaccatg gaggatctca agaagatcct
      421 ctacatgatt tgattaacca tcaactgaag tggcaaacag ggggttgtac gccggaacca
      481 caaaagacgg atttataggg gcaaatccga ggagaagacc caatccgcat ggttagaatc
      541 tataggttgc actaatttta acatttcttg gttctccata tgtttccgtg ccatatccaa
      601 tcgattcacc tatggatgta taataaacct tatcaaagac attagttata ttgagctgta
      661 aatccagttg tggcattgga gtatagccaa taataatgtc agttgttgaa taagcctttt
      721 gtttattaat aaccggattg ccatcccttg tgtctgttat gttagcatat attttacttt
      781 gccagtacgt gtttactcct gctcgccagt ttgttaattt tccgtcaaag gtgtacatgc
      841 tcgaaatctt aaataaattt tgtggcgaca cggcagtatt gaatcgcttg ccttcattac
      901 tacgatctcc atcttttata tatcgcgtat ctgcatatgt atatcccacc ccaatttccc
      961 agagtggcgt aaccatgccc tgaagttcga aatctatccc ttttgtgcgt actttaccgc
     1021 tagcctcata gcattgagga ttgccattag taacacaccc tgtctgatcg ggaagcagcc
     1081 tggctcgatt ttcctggtct acctgaaata ccgccagaga ggcattaagc aagccgttaa
     1141 aatactcacc cttaatacct gtctcataat tcttaccaac aataggcttg atcactttac
     1201 cattgaggtc ggtgcttgac tgtggattga atatatcggt atagctccca taaatggtat
     1261 gatgatcgtc aagattgtat gttgaacctg catatttggt gatatttctg gtaactttat
     1321 aacctgtatt aatggatgta cctttacttg tatattcata ccagtcaaga cggccaccca
     1381 gcaatatttt ccagttatca ctaagtagaa aatgagttgc tgcgtaggcg cttttttgtt
     1441 cagtgacgtc gtaggatgca tcacctgaca tcttcatggg ttttttcgaa gaatgtgaac
     1501 catgatcaaa tatattaaca ttctcaatat atattgggga atatgtataa cctgtatctt
     1561 tatcggtgta tttttgctga gttgttccag ccacgaattg atgaacacga ccaaataaag
     1621 catagtcccc attaagcgac aggtcatagc ttttggaatt atattccctc agtgatcccc
     1681 atacttgctg atacatactt tgtcgggtat ctttatgccg gtatgttgcc gtccctaaca
     1741 ggtcagcatt tctccagttt tgcagcgttg aaaatttcag cttccagcta ttactgaact
     1801 tctgctcaag agttgcaaat gcagttttac ttttacttct ttcaaactcc cagtcattgc
     1861 ctgtaaacgt cgagcgagga agattaaggt ggtgtccggt accagaaata ggtaagtcat
     1921 accaccaatg attgttatat tcatactgct gatatccccc aatcgtaagt agggtattat
     1981 cagtcagatc tgcctccata attccgtaaa tgacgttatt cctgcgtttc tcactatccc
     2041 tgaaggtctc actgttttcg ccatccacaa ctgcccttcc gcgtaaggtc gcgctgtcat
     2101 tcagtggtcc tgatacatca accccacccc ggtagtcatt ccaccgtccg gcaccgatgt
     2161 tagtgctggc ctgatattgc cgggtcggac gcttgtgtat catatttatc gcggctgaag
     2221 ggtctccggc accctgaaca agacctgttg ccccgcggac tatctccacc cggtcaaaaa
     2281 tagctgaact agtttgtgaa cttggaatgt atgaattcca cggcaggcta aatccttcat
     2341 acatgacatt agcattaaat cctctggaca taaaattcgg tcttccggaa ccttctgctg
     2401 tactgacata taatccagga gcggttttaa ccacatcaat aatatttttt gctgctacat
     2461 catccattct tcgtcgcgta atgactgtag ttgactgagg tgtatcccgg aatgacagat
     2521 tcatctttga tgctgtattg gctgactggg ttgtgtatga atctgttcct tctgttgtgc
     2581 tttcagaaac agcggtccca tgaacaatta atgtttcttc tggtgaaata tttttatctt
     2641 tactaactgc gccagaatca tcagccataa cagatgatgt atacatcata tatactatgc
     2701 tcagccctaa atatttattc attcttctgg ctccatagca attttagata attcgatttt
     2761 gagtatgtaa tccttcatag tactcatctc ttttttttct tgttttacac taacgtacag
     2821 attttcaccg tcatgtgata aagacagact gtttggcatc aggcgggttt ttatcgttgt
     2881 ttttacgtaa taagaattac tgtccacaat gctgacctgt tttgcattac ggtgcgtgat
     2941 atagatttcg ttccgtaaag gattaaataa aatcgccagt gaatttataa catcaattct
     3001 gtgtagtatt tttcccgagt ttacatcaac aaccagcacc ccgggaatat ccgggtcggt
     3061 aataaatgca cgtccgtttt tttcatccag acttacatta aggaaaaagt gttttttctc
     3121 aggctctaca acaaaccgtt tttttatttc atagctactg gcatccaggt taataatctc
     3181 accatccccg ttaaccagat aaatattatc cccattttta tttaacgcaa ttccagtaac
     3241 agcgtgcccc agtccatcaa tggttttaat tttcttcccg acagccatgt caataaccca
     3301 cagagcccct ttatctgcgg cgctggagac ataaagacgc tgattttttt tgtcaatgac
     3361 catttctctg gtcggtctga aatgagcctc atcctgtccc tcagttaacg gaattactga
     3421 aagttctttt ccggttagag tatcaatgac cgtcactgag ccgtccagag tattgccgat
     3481 ataaagtcgc tcatgttcct catccagtgc tgtggcaaac ccaagacgtg cgattttaac
     3541 ggcagatttt gttttcagag tggatggttc taactgataa acaattccgt ggcccttatc
     3601 tctgtcgaat gaagacgatg atgctgccca gagactatta tttttgccat caaaagacat
     3661 ttcatacaga ccattgccga tatcacgagt caggaatgtt gattcagcta tatcagccgc
     3721 atacgctgaa ccagtaaaca gtagtaaaga gtatattaat actctgctac ggacattaac
     3781 ggcggattta atcgtgattg ccatacttac atttccttac aataaaagaa acaagaatat
     3841 agcaaaagtg ataatgatta tcactaccat taatcacatg atttaccata acgtcgcatt
     3901 acgcgcaccg ctcagacggt ctgagggcat ttccggcggc tacgggataa aaccggcaac
     3961 aaaccacccg gaaaaccgcc ggaaacgcgc ctgataaatt ttaacccgct gcatggctat
     4021 gcacgcaact gcatgtcgtg cagtttgacg taaaatgggt gtcgtaagcc cttttttacc
     4081 agctttcgtc ttcatcagcc tgactgacac gagcatattc tttcagaccg ccccctgcta
     4141 aactccttca tatcgacgta tcagcccggg aggctccaca atgagcaaca gtacacacta
     4201 tgaaaacgcc aatttcctgc gggaactggc cgagagactg ccccgcatac tgcctgaagg
     4261 tggcccggac aaagctgcac tgttgcagcg cctggctaac gaagaactgg cccaggcaga
     4321 atatgaagat caggttcgcg ctaaagtgac cgccgcccgc gctgacacgc gcccgggcat
     4381 gaccacggaa caactccgcc agagactgca tggccgctat caggaactac gcgatgccgt
     4441 atgaggtgga atggaagcag ggagccatcg aggatgtgac agcattgttt gactacattg
     4501 cggaaaattc atccctgtgg gatgcgcaga acgttaccga tcgagttctc gctgcagcgg
     4561 acaaactggc cgacttcccg cgcctatatg aagtggactc acgctatggc gaggacgttc
     4621 gccggatcag tctgatggga cagcatgtcc tctatgatgt tgatgaccag accaggaaag
     4681 tccgggttct ggccgtggtt ggccagcgcc agaatcccca taccgttcgc tgataaccgg
     4741 tgatgggggc gcaattcatt gcgtggccgt cgccggttgg acaagcacag cgcgtcagcg
     4801 ttttccccac tgccaccatc ggcgcgatgt tccggccggt gtctctgctg tctctggttt
     4861 gtgttccagc agtctcatgg cctgcatttc ctgccgcagt ccggccacct cctccctgag
     4921 tgcttttatc tccgcgatca acgcgtcgga ggccattttt tcagtctcat ccgacagtct
     4981 caacccgtcc cgctcgtgag tctcaatctg tcttaattca ccgtacaccc ggataagttc
     5041 gctggtatcc accacccgac caccatcagc ttcagtacgg tatgatacgc ggcctttcgc
     5101 catatcccta tacagggaac tccgggcttt ccccgtcagt tccgtcgcct gtctcaccgt
     5161 cacccacgcc attgtctcac ctccggtttt gagacagtat acccgacagg aaggtaagcg
     5221 actcgtcaga accgtattga tatttactga gagctcagat caactttcca gggcaacaga
     5281 tcgcgtaccc ggtttgccgg ccagtcctgg atatgttcaa tgacgtaacg cagccacttt
     5341 tctggctcca cattgttcag acggcatgtg ccgatcagcg agtacaacac cgccgcatgt
     5401 tcaccaccgc tgttggaacc cgcgaacatc cagtttttcc ggcctacggc cactccccgt
     5461 aaggcgttct ctgcgatgtt gttgtcgatt tccacccagc cattactgca gtacacgttc
     5521 agtgcatccc actgtttcag caggtatgcg aacgcttttg ccgtatctga gtgacgcgac
     5581 agtgttttca tctgttgctg tatccagtca tacagtgact gcatcagtgg cgcggctctg
     5641 gcttttcttg ccgccagacg ctgttctgct gaacagcccc ggacctctgc ctcgatggca
     5701 tacagttcac cgatacgctg cagggcttcc gtggtgatgt aggtgggcgc tcttgcatgc
     5761 acatcgtgga tttttctccg ggcatgagcc atacacgcgg cttccgttat tctgccggat
     5821 tcgtataacg cccggtaacc accgtaagca tcggcctgaa gcacaccgct gtaaccggcc
     5881 aggtgatttt gtggatggat acctttccgg tccggactgt acgcgaacca gaccgccggg
     5941 ggcatctgtg aaccggcgtt acggtcatca cggacgtaga cccacagccg ggctgtccgg
     6001 gttttaccgc tgcccggctc ctggaccggg acggggatat catcagcatg gactttaccg
     6061 ggcatcagca catactggcg caggacgtca tacagcggct ccagcagttc agcaacagca
     6121 cctgtccagc gccccagtgt ggcacggctc agctccactc cctgacgacg gtatatttct
     6181 gactggcggt ataacggcag atggtctgca tatttcccgg tgacaacatg ggccagaagc
     6241 cccgctccgg cataactgcg tgcaatgggt tttgaaggta ctggtgcctg cacgatatgg
     6301 tcgcaccggc aacaggcctg tttcggacgt tgtgtttcga taaccttaaa ggcgctgctg
     6361 ataagctcca gttgctctga cacatcacat cccagagaac tgagttcacc accacaggca
     6421 ggacagcatt cctcttccgg ccggataacc cgggtttcac ggggaagtga ggccggtaac
     6481 ggtttacggg ctgaagactg gcgcagggcg gatggcagta ccgggtcata ttgctcaccc
     6541 agcgtttccg ccatttcttc ctgaagtgcg ctgattcgct cctgtgcttc ctgtatctgc
     6601 cgttcggttt ttgcacgaag tttttctgag cttttaccga actgcatacg ttgcagtttc
     6661 gcaaccagcg ccttcagccg gttgatttcg gaagcataag ccgccacccg ctgtgagagc
     6721 aggcggttgt attcagccat ctggcggatg gtgtcctgtt gcgtctgcaa cagtgcccgc
     6781 aggcgggcgt tctcatgagc aagtgaggtg tccatatcct cactttacaa cgggttatat
     6841 gcggattcca gcgcgttccg ttcgtttcgg gtgcttccag ttgatacctt caagaagcat
     6901 ggataactga gccggagtaa ggtgcacctt gccgtcacgg gtgactggcc agacgaagcg
     6961 gccccgctcc aggcgtttgg tgaagaggca cagtccgtca ctgtcagccc acaacacttt
     7021 tatctggtca ccccggcgtc cgcggaagat gaacaggtgt ccggagaacg ggtcatcctt
     7081 caggacgttc tgaacttttg atgccaggcc gttaaagcca tttcgcatat cggtgatacc
     7141 tgcaaccagc cagatacgcg aacctgcagg gagagatatc atcagtggct gctccctttt
     7201 atttcgcgga taagtgtctg taataacgcc ggcgtcagtt tacctttaag cctgagagtt
     7261 ccggccggca gaaccagctc acaacacaga ctgtcggacg gtgtatttat ctgctctggt
     7321 tcctgtgcgg gggccgggat tttattatcc ggctccggcg ttaacgtcac gggaagcagt
     7381 gccggcatat tttttccgga aggcagcagg ccacctttcc ggtattgatg gcgccagttg
     7441 aagagcaggt tatcgttgat tccgttttcc cgggcgatct gcgccacaca ggctccgggc
     7501 tgcagtgact gctccactaa ggcgatttta aactcataag ggaagttggg ccgccgggga
     7561 cgttttttta ccacgggggc ttcggatata acggtgcttt caggacgtac gactggtacc
     7621 gtggaaaatt gtccgtaaag gcaggcatca agttcctgct ccgacatgcc tgcgggcaaa
     7681 ggccacgaaa ggccagctct ccgaaagcgc acgaacatac tacaaactgt tgattttggt
     7741 acacccaggc gacgcccggc cacaacccgg ggtaaatgtt cttcaaagtg aagacgtaaa
     7801 gcttcagtga tccaggtccg gtgtttcata cgatagtgtc cattaaaaat gatggacatt
     7861 atttttgtag agccggagga aacagaccag acggtttaaa tgagccggtt acacaggaag
     7921 cacaccacac ccggaaggac agaaaacatg actaagcagg aaaaaactgc cctcaatatg
     7981 gctaaattta ttaaggacca gtcgctgctg ttactcgaga aactgaacga gctggatatg
     8041 gataccccgg ccgacatgtg cgagcggctg cacgaggatg cggaaacact ctggcaggtg
     8101 atgcgggata agctcgggaa ggaataacag caccggcatg gccggtgctg ccgtttattt
     8161 tttgccgaac ggccaccacc ggcgggcggg tttgtcctcc ggcagttcag gcaccggcac
     8221 gttaaaatag cggctgttat attcccgcat cagctccgct ttcagcttct cgttcaccgc
     8281 gctttccccg tgccgctcgc tgaccacctc aatcatcgcg ctaatctgct ccggtgagca
     8341 cttcccggca tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca ccttgcgata
     8401 gaggtaatca cacagcttca ttcctgcggg tttgtcccgt tcccattgcg gatttttacc
     8461 ggtgaaaaag tacgtggctt tttcgtcgct gttgagccgc tcgtcataca tcccgatgga
     8521 tttcatcatc gccagatgct gggcccggtc gacaaaacag aaaacatacg ccccggtctg
     8581 ccaggcgagc accaccatat cttcaagatg ctcgctggtc ggcatgatgc agcgctcgcc
     8641 gtcccggtcc tccgcgtctt caataaatac catgtacagc gccataatcc ctcactccgt
     8701 tgtcataaat ttaaagcacg caaacagccg ctcccggtcc agctccgccg ttttgcgggc
     8761 ctcgccggtc tgtggattca cgatttcatc caccgacagg atcgcccgct gcaccctgac
     8821 ctgcgtcgcc cggtccgggc agatgtacca gatttcatcg atgccgttcg ctttgcgatt
     8881 gaacagatac ccggacacca cctcctgata gcgcctgcgg ctcttcacgg tgcgctccac
     8941 ctcaaacgcc accgttctgc cgtccagctc cgccagcgcg tccggcacct tcatcccctt
     9001 cagggccata cggtgcaggt accgccagcc ggaggccccc cgggcctcca tggcgagacg
     9061 cagggactgc acggccagcc ggtgcggcag ctgcgccgcc gacacccttg acggctgaaa
     9121 cggaataacg tccgtcagcg gttcgtcctc gtcgaacgag aacgccaccc cgtgcggcgt
     9181 cagtccccac acgctgacct gacggccgtc aaccgggtat ttcgcccgga ccagcagccc
     9241 tttcgccacc aggctgtcca ggaagcggta aaccggctgc accgccgcaa tccccagccg
     9301 tcgcgcaacc agctccgccg tggaacacag ctcttcccgc agccagcgca gggtcagccg
     9361 ggttttctcc gcgttccgcg cctgccgctt cgcgtggctg acgttgtatc gctccagtgc
     9421 catcagtaca gccccgcaaa atcatcatca cccggccgct gccgcccggc ctccggctgg
     9481 tactccgcca gcgggtccgt tttcgccagc accgggaagc gctgcagcgg gatttcccgg
     9541 cgttcaaccc gcagcgggct gctgaacgcc atccgggcca cgcccagccc ggtcatcacc
     9601 gcaaacccgt caggcaggtg ctgcagggtg ttcacgtcaa acagcggccg cgcctccttc
     9661 tgaatgaagc tgtccccgct gatatgctcc acgttccccg cctcccggct ggcccggcgg
     9721 cgctccacgt ccacccggat ttccccggtc tggcccgccg cccactgcgc ggactcctgg
     9781 ctggccgccc ggtagaacca gcggatcggc gtgttatcca gcaccgtggt tttcacaaac
     9841 tcagcgggca gatcctggcc gcactgcccg aagtcgccca gcgactggtg cgcgagcagc
     9901 aggttgcagt cccgactgcg cagcgtcccc agcgcgttca gcacgtactt cgacagcagg
     9961 tatttaatct catcgagcat gatgctcgcg tgcggccacc tccggaactc atcccgcgca
    10021 atgatgattt gcgcgcagcg ggcaaacagc atcttctgca cccggatcac cgcctcatcg
    10081 tccatcgagc cgatgacgta caggcagccg ccgccgttga ttatccccgc cacgtcgatg
    10141 ccgctgtccg tctgcaggac gctcaggtcg gccacgttct ccagcttggt gatcagcccc
    10201 ttcacgtcct ttttcagggc ctccggcagc gcgtatgccg ccgccagcac ctgctgaatg
    10261 tttgccccct gcgggaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg
    10321 tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag cagattcttc
    10381 agggcgtact ggtcgagatc gcgaaacaga ttcagctgcg ccggtttccc cggccgcaga
    10441 tccagcaggg tgaagtccgg gcagtgcgcc cggaagacgt gcggcgccca cgcgtcccct
    10501 ttggggtcga agtacaccac cgcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg
    10561 gtgcccatca cgctcttgcc gctcccgggc aggccgagga tctgaatatt ggtcttgcgc
    10621 caggtatcac gcgggaccag caccggctgg ccctgctcat ccttacccag aaacaccgcc
    10681 tgctgtttcc gggcctgctc tgtggaaaaa tcggccagcg cgttaaccgg taccggctcc
    10741 ccgaaatgca ggtggcggac gtcggtcatt tctccggtcc gctggctgct gcgccggctc
    10801 tcctggttca gtttcgcatt caccgcctcg tcagcggccg caaaatgacc gtcaagcagc
    10861 agaaatgcca gcagccgcgc cagaaggatg ccggcgacaa gccctccggc acaccagaca
    10921 atcccgaaca ggttagccgg ctgcagcagg taataatctg cgttcgcgta gaggtccgga
    10981 cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg
    11041 gccagaccgg cagcgccggt cagccagtac gcgtagttcc agacatgccg ccgtgccctg
    11101 accatccgcc cgggcgaatc cagcgaagcc agcaccgaaa atggcgcagt ccggttatta
    11161 tcgcgcttat agcgatcggc atactcccgt cgctccttat gactcagccc cacaaaacgc
    11221 atccgtccgg cccagagttc agccagaccg aaaaacaggc cgataaccag gaaagccccg
    11281 gccagaccgt aatactccct gtacagccag ctgacccacg ggtaaatctg ccgggccagc
    11341 tccgggatat gccactgcca ggcaagcgaa tccgtattaa acatgcaatc tctctccttc
    11401 agacgacggg tttaccggcg tggtgtgcag cccctgctgc acaagccgcg gacatgcccc
    11461 gaggcatggc ggcggcggtc acgtccgtgg tgattgcatt cagctttcac cacggaggcc
    11521 agcctctttc cgccccaagc ggaaaaggcg cagccccgga ggaacaacga gcacgcccca
    11581 gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc ggcccccgtc
    11641 agagttcgcc atagcgagcg gctgaccacc cgcaaaatca ggagcagctt tgctgcttcg
    11701 tcaggtgcgt tcagcaccgt gggtttttgt gggtggtcag gcgtgagcct gacgggggcc
    11761 gggtgaattt ctggcgtggc cggcgtccag ccgagctgag ccagaaagag ggggaaagac
    11821 cgtgcgtcgg tggcgtccag ccagcgtaag caggggattt cctcctgaag tcagccacgc
    11881 cccagcgggg atgacctgca tttctccgca aggagaaaaa agcaacagag ataggttaac
    11941 gtaaaatatt cagcagaaaa ccgacagact tcacctatct gaatgattta tcggcaggga
    12001 agggaaaggc ggtaacacgg tgcaatatgt cacatccgcg cgtccggtgg ccgtctgcgg
    12061 gaggggggat attgcgcctc ctccctcact cagtcgctac gctccggccg tgagactgcg
    12121 gcgggcgctg ctaactctcg caaagttacc cacagattcc gtggataagc aggggactaa
    12181 catgtgaggc aaaacggcag ggccgcgccg gtggcgtttt tccataggct tcgccctcct
    12241 gccagaattc acataaacag acgctttttc ggtgcatctg tgggagccgt gaggctcaac
    12301 catgaatctg acagtacggg cgaaacccga caggactata aagataccag gcgtttcccc
    12361 ctggtggctc cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga tacctgttcc
    12421 gcctttctcc cttac
//
