LOCUS       NZ_CP092281             8801 bp    DNA     circular CON 28-JUL-2023
DEFINITION  Klebsiella pneumoniae strain T877-PC plasmid p3, complete sequence.
ACCESSION   NZ_CP092281
VERSION     NZ_CP092281.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25889007
            Assembly: GCF_030544725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Liu,C., Dong,N., Zeng,Y., Lu,J., Chen,J., Wang,Y., Chen,G., Shen,Z.,
            Chen,S. and Zhang,R.
  TITLE     Co-transfer of last-line antibiotic resistance and virulence operons
            by an IncFIBk-FII-X3-ColKP3 hybrid plasmid in Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8801)
  AUTHORS   Liu,C., Dong,N., Zeng,Y., Lu,J., Chen,J., Wang,Y., Chen,G., Shen,Z.,
            Chen,S. and Zhang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2022) Department of Clinical Laboratory, Second
            Affiliated Hospital, Zhejiang University School of Medicine,
            Department of Clinical Laboratory, Second Affiliated Hospital of
            Zhejiang University, School of Medi, Hangzhou 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 17:24:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,327
            CDSs (total)                      :: 5,209
            Genes (coding)                    :: 5,120
            CDSs (with protein)               :: 5,120
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 39 of 89
            Pseudo Genes (incomplete)         :: 49 of 89
            Pseudo Genes (internal stop)      :: 15 of 89
            Pseudo Genes (multiple problems)  :: 10 of 89
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092281.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="T877-PC"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="China: Zhejiang"
                     /collection_date="2021"
     gene            complement(join(8771..8801,1..269))
                     /locus_tag="MHK77_RS26585"
                     /old_locus_tag="MHK77_26545"
     CDS             complement(join(8771..8801,1..269))
                     /locus_tag="MHK77_RS26585"
                     /old_locus_tag="MHK77_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094313765.1"
                     /translation="MPYEVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(259..522)
                     /locus_tag="MHK77_RS26590"
                     /old_locus_tag="MHK77_26550"
     CDS             complement(259..522)
                     /locus_tag="MHK77_RS26590"
                     /old_locus_tag="MHK77_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_094313764.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESGPDKAALLQCLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(828..1073)
                     /locus_tag="MHK77_RS26595"
                     /old_locus_tag="MHK77_26555"
     CDS             complement(828..1073)
                     /locus_tag="MHK77_RS26595"
                     /old_locus_tag="MHK77_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1400..3085
                     /locus_tag="MHK77_RS26600"
                     /old_locus_tag="MHK77_26560"
     CDS             1400..3085
                     /locus_tag="MHK77_RS26600"
                     /old_locus_tag="MHK77_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_214301846.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARTAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3095..3352
                     /locus_tag="MHK77_RS26605"
                     /old_locus_tag="MHK77_26565"
     CDS             3095..3352
                     /locus_tag="MHK77_RS26605"
                     /old_locus_tag="MHK77_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3437..3586
                     /locus_tag="MHK77_RS26610"
                     /old_locus_tag="MHK77_26570"
     CDS             3437..3586
                     /locus_tag="MHK77_RS26610"
                     /old_locus_tag="MHK77_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5069..7030
                     /locus_tag="MHK77_RS26615"
                     /old_locus_tag="MHK77_26575"
     CDS             5069..7030
                     /locus_tag="MHK77_RS26615"
                     /old_locus_tag="MHK77_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_032448356.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7030..7761
                     /gene="mobC"
                     /locus_tag="MHK77_RS26620"
                     /old_locus_tag="MHK77_26580"
     CDS             7030..7761
                     /gene="mobC"
                     /locus_tag="MHK77_RS26620"
                     /old_locus_tag="MHK77_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_094313769.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMMMD"
                     /name="mobC"
     gene            7790..8053
                     /locus_tag="MHK77_RS26625"
                     /old_locus_tag="MHK77_26585"
     CDS             7790..8053
                     /locus_tag="MHK77_RS26625"
                     /old_locus_tag="MHK77_26585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094313768.1"
                     /translation="MTNRVVAVGREALLMVAVMAGTLVVLAGNLLKLLWRVLPVLLLLY
                     GVVQFLARNPEIRDWLFLHFGQGGTAAILVGGILLCMRRLMK"
                     /name="hypothetical protein"
     gene            complement(8084..8263)
                     /locus_tag="MHK77_RS26630"
                     /old_locus_tag="MHK77_26590"
     CDS             complement(8084..8263)
                     /locus_tag="MHK77_RS26630"
                     /old_locus_tag="MHK77_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094313767.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     EKLWLAMRDHLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8332..8709
                     /locus_tag="MHK77_RS26635"
                     /old_locus_tag="MHK77_26595"
     CDS             8332..8709
                     /locus_tag="MHK77_RS26635"
                     /old_locus_tag="MHK77_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_094313766.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVMDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTAFDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="entry exclusion protein 1"
     BGC             1..8085
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3690..4268)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7031..7758
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.004"
                     /coverage="100.0"
                     /evalue="1.97e-140"
ORIGIN
        1 ccaaccacgg ccagaacccg gactttcctg gtctggtcat caacatcata caggacatgc
       61 tgtcccatca gactgatccg gcgaacgtcc tcgccatagc gtgagtccac ttcatagagg
      121 cgcgggaagt cggccagttt gtccgccgca gtgagaacgc gatcggtaac gttctgcgca
      181 tcccacaggg atgaattttc cgcgatatag tcaaacagcg tcgttacatc ctcgatggct
      241 ccctgtttcc attccacctc atacggcatc gcgcagttcc tgataacggc catgcaggcg
      301 ctggcggagt tgttccgtgg tcatgcccgg acgcgtgtca gcacgggcgg cggttacttt
      361 ggcgcgaacc tgatcttcat actctgcctg ggccagttct tcgttagcca agcattgcaa
      421 caacgcagct ttgtccgggc cactttccgg cagtatgcgg ggcaggtttt cagccagttc
      481 ccgcaggaag ttggcgtttt cgtagtgtgt agtgttgctc attgcggaga ctcccggatt
      541 aatggggtga tatgaagaag tttagcaggg ggcggtctga aagaatatgc tcgtgtcagt
      601 caggctgatg aagacgaaag ctggtaaaaa agggcttacg gcacccattt tacgtcaaac
      661 tgcacgccat gcagttgcgt gcataggcat gcattaggat aaagtttacc gggcgcgttt
      721 ccggcggttt tccgggtggt ttgttgcctg ttttatcccg tagccgccgg aaatgccctc
      781 agaccgtctg agcggtgcgc gtaatgcgac gttatggtaa atacccctta ccggcggcgc
      841 tgtggctgcc aggtgaaggt ggtttccgta gcctcgtatt tctcccgacg catttcctgc
      901 gcccgcctgg cttcacgttc gtcctgaatg gcacgaagca ccctttcccg cacctgttgt
      961 tttaccgcct ttggcagcgt tttgttccct ttcaggccca tggccagtgc ctgagcgaca
     1021 aaggcgtccg ccaggcgctt ttcttcagcg ggagtcagtt tcaggtgcgt catagtggcc
     1081 tccgggcgtc tcaggtttcc cgaacagttt aaccgcaggt cctctcctca tgccagttag
     1141 ctttcagatg tgtctatacc atctgaaatg taagtgtgat gaatgtctta aataataaaa
     1201 tttgttgcca tgagaaattt tcgggaaaag acgcgatctg cttacaggtg gctccgggcg
     1261 cttttttgtg cctgaagatg agaactaatc tcatatattg acacctgaaa acgggaggag
     1321 taaggtaata attatactgt gtatatgtac agtatgttgg ctttaattta ttgttttaaa
     1381 tgtcaaagag gaaaacgata tgagtggtgg agacggtcga ggtccgggta attcaggtct
     1441 gggacataat ggtggtcagg ccagtgggaa tgtgaacggt acgtctggta aaggtggccc
     1501 ttcatcaggt ggtggtacgg atccaaacag cgggccgggc tggggtacga cgcatacgcc
     1561 gaatggagat attcataact ataatccggg ggagtttggt aacggcggga gtaaacccgg
     1621 tggtaatggt ggtaacagcg gcaatcatag cggtagctct ggtggcggac agtcttcggc
     1681 cactgcgatg gccttcggtc tgcctgccct ggctactccg ggggctgaag gactggcttt
     1741 atcagtttcc ggcgatgcgt tgtcggccgc cgttgctgat gtgctggctg ccctgaaggg
     1801 gccgtttaag tttggtctgt gggggattgc gatctacggt gtgctgcctt ctgagattgc
     1861 aaaagatgat ccgaaaatga tgtcaaaaat tatgacgtca ttaccggccg atgcggtgac
     1921 ggagactccg gcaagtactt taccactgga ccaggcgacg gttcgtgtca gacaacgggt
     1981 tgtggatgtg gtgaaggatg agcggcagca tattgcggtt gtcgcaggtc ggccaatgag
     2041 tgtccctgtg gtggatgcga aaccgacaaa acgtccgggg gtattcagtg tgtcgattcc
     2101 gggtctcccg tctctgcagg tgagcgtacc gaaaggtgtt ccgacagcga aagccccgcc
     2161 aaaaggcatt gttgctgaaa aaggtgattc acgtccggct ggttttacag ccggtggtaa
     2221 ctcccgtgag gccgttattc gtttcccgaa agagaccgga cagaagccgg tttatgtgtc
     2281 ggtgacagat gttcttaccc cggcacaggt aaaacagcgt caggaggaag aaaagcgtcg
     2341 ccagcaggca tgggacgccg ctcatccgga agaggggctg aaaagagact atgataaagc
     2401 gaaagctgag ctggatgccg aagataaaaa tattgcgacc ttaaacagcc gcattgcatc
     2461 gacagagaag gcgctccccg gtgcaaggac tgctgttcag gaagccgata aaaaggtaaa
     2521 agaggcagag gcaaataaag atgattttgt gacttataac ccgcctcatg aatatggctc
     2581 cgggtggcag gatcaggttc gctatcttga taaggatatt cagaatcaga atgagaaatt
     2641 aaaagcggct caggcatctt taaacgcaat gaatgaatcc ttatccagag ataaggctgc
     2701 acttaccggg gcgatggaga gccggaaaca aaaggagaaa aaagcgaagg atgcagaaaa
     2761 taagttaaat gaggaaaaga acaagcctcg caaggggact aaagactacg gccatgatta
     2821 tcatccagta cctaaaacag aagacataaa gggacttgga gagttaaagc gaggtgatcc
     2881 taaaacacct aaacaaggtg gtggtggtaa gcgggcccga tggtatggcg ataaaaaacg
     2941 taaaatttat gaatgggatt cccagcacgg tgagcttgaa gggtatcgtg ccagtgatgg
     3001 cgaacaccta ggcgcatttg atcccaaaac gggtaagcag gttaaagggc cagatccaaa
     3061 acggaacatt aaaaaatatc tttgagaggt aaatatgggg cttaaattac gtttgacatg
     3121 gtttgataag aaaactgaag agtttaaagg ggaagagtat tctaaagact ttggtgacga
     3181 tggttctgtc attgaaagct tgggaatgcc tttaaaggat aatattaata atggtgagtt
     3241 tgatttgaag aaagaatggg ttcctttact acagccattt tttaaaaatc aaataaaacc
     3301 tgatgtggat aattgttata tagcttttga ttatcgggac ggtaactggt aacaaaaaat
     3361 tatcaaggat gatttgaaat actgacttcc ggtgttcagg gatgaatgcc ggagtcttta
     3421 aatacaggga aagtatatga aaaaggtaaa gactattttt ctttttatat tgattgttgc
     3481 tggctttctt ctggccgcat gtcaggcaaa ttatatccgg gatgttcagg gcggaacggt
     3541 ggcaccatcg tcctcctctg aactgacggg gatcgcggtt cagtagaaaa gatcaaagga
     3601 tcttcttgag atcctttttt tctgcgcgta atcttttgcc ctgtaaacga aaaaaccacc
     3661 tggggaggtg gtttgatcga aggttaagtc agttggggaa ctgcttaacc gtggtaactg
     3721 gctttcgcag agcacagcaa ccaaatctgt ccttccagtg tagccggact ttggcgcaca
     3781 cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg cgaactccca gttaccaatg
     3841 gctgctgcca gtggcgtttt gcgtgctttc ccgggttgga ctcaagtgaa cagttaccgg
     3901 ataaggcgca gcagtcgggc tgaacgggga gttcttgctt acagcccagc ttggagcgaa
     3961 cgacctacac cgagccgaga taccagtgtg tgagctatga gaaagcgcca cacttcccgc
     4021 aagggagaac ggcggacagg tatccggtaa acggcagggt cggaacagga gagcgcaaga
     4081 gggagcgacc taccggaaac ggtggggatc tttaagtcct gtcgggtttc gcccgtactg
     4141 tcagattcat ggttgagcct cacggctccc acagatgcac cgaaaaagcg tctgtttatg
     4201 tgaactctgg caggagggcg gagcctatgg aaaaacgcca ccggcgcggc cctgccgttt
     4261 tgcctcacat gttagtcccc tgcttatcca cggaatctgt gggtaacttt gtatgtgtcc
     4321 gcagcgcccg ccgcagtctc acgcccggag cgtagcgacc gagtgagcga ggaggcgcaa
     4381 tttccccggc acctgcaaga cggtcaccgg acgcgctgat gtgggatatt gtcccctgat
     4441 taagtctttc tattccctga cgatcaatta tcaggatagg tgaaccatgt cggtttttcg
     4501 ctgaacattt tacgttaacc tatctctgtt gcttttttct ccttgcggag aaatgcaagt
     4561 catccccgct ggggcgtggc tgacttcagg ggaaaagacc ttgcttacgc tggctggacg
     4621 ccaccgacgc acggtctttt cccctctttc tggctctgtc cggctggacg ccggccacgc
     4681 cagaaattca cccggccccc gtcaggctca cgcctgacca cccacaaaat ccacggtgct
     4741 ggacgcacct gacgaagcag caaagctgct cctgattttg cgggtggtca gccgctcgct
     4801 atggcgaact ctgacggggg ccggaacacc ggaaaagacc gcactggggt gccgcctttt
     4861 cctctcacac gctggggcgt gctcgttgtt cctccggggc tgcgcctttt ccgcatgggg
     4921 cggaaagagg ctggcctccg cggtgaaagc tgaacgcaat caccacggac gtgaccgccg
     4981 ccgccatgcc ttggggcatg tccgcggctt gtgcagcagg ggctgcacaa cacgccggta
     5041 aacccgtcgt ctgaaggaga gagattgcat gtttaatacg gattcgcttg cctggcagtg
     5101 gcatatcccg gagctggccc ggcagattta cccgtgggtc agctggctgt accgggagta
     5161 ttacagtctg gccggttttt ttctggttat cggcctgttt ttcggtctgg cagaactctg
     5221 ggccgggcgg atgcgattcg tggggctgag tcataaggag cggcgggagt atgccgatcg
     5281 ctataagcgc gataataacc ggactgcgcc attttcggtg ctggcctcgc tggattcgcc
     5341 cgggcggatg gtcagggcac ggcgacatgt ctggaactac gcatactggc tgaccggcgc
     5401 tgccggtctg gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga
     5461 aaccgtcctg cgtccggacc tctacgcgaa cgcagattat tacctgctgc agccggctaa
     5521 cctgttcggg attgcctggt gtgccggagg tcttgtcgca ggcgtcctcc tggcgcggct
     5581 gctggcgttc ctgctgcttg acgggcattt tgcggccgct gacgaggcgg tgaacgcgaa
     5641 actgaatcag gagagccggc gcagcagcca gcggaccgga gaaatgaccg acgtccgcca
     5701 cctgcatttc ggggagccgg taccggttaa cgcgctggcc gatttttcca cggagcaggc
     5761 ccggaaacag caggcggtgt ttctgggtaa ggatgagcag ggccagccgg tgctggtccc
     5821 gcgtgatacc tggcgcaaga ccaacattca gatcctcggc ctgcccggga gcggcaagag
     5881 cgtgatggcc accaacgcgc tcattcgctg cgtacgcgat ttcggggacg cggtggtgta
     5941 cttcgacccg aaaggggacg cgtgggcgcc gcacgtcttc cgggcgcact gcccggactt
     6001 caccctgctg gatctgcggc cggggaaacc ggcgcagctg aatctgttcc gcgatctcga
     6061 ccagtacgcc ctgaagaatc ttctggttgc cggatttaac ctgagtgaga ccggcgacgt
     6121 ggcggaccac taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagtttcc
     6181 gcagggggca aatattcagc aggtgctggc ggcggcgtac gcactgccgg aggccctgaa
     6241 aaaggacgtg aaggggctga tcaccaagct ggagaacgtg gccgacctga gcgtccttca
     6301 gacggacagc ggcatcgacg tggcggggat aatcaacggc ggcggctgcc tgtacgtcat
     6361 cggctcgatg gacgatgagg cggtgatccg ggtgcagaag atgctgtttg cccgctgcgc
     6421 gcaaatcatc attgcgcggg acgagttccg gaggtggccg cacgcgagca tcatgctcga
     6481 tgagattaaa tatctgctgt cgaagtacgt gctgaacgcg ctggggacgc tgcgcagccg
     6541 ggactgcaac ctgctgctcg cgcaccagtc gctgggcgac ttcggccagt gcgggcagga
     6601 tctgcccgct gattttgtga aaaccacggt actggataac acgccgatcc gctggttcta
     6661 ccgggcggcc agccaggagt ccgctcagtg ggcggccgga cagaccgggg agatccgggt
     6721 ggacgtggag cgccgccggg ccagccggga ggcggggaac gtggagcata tcagcgggga
     6781 cagcttcatc cagaaggagg cgcggccgct gtttgacgtg aacaccctgc aacatctgcc
     6841 tgacgggttc gcggtgatga ccgggctggg cgtggcgcgg ctggcgttca gcagcccgct
     6901 gcgtgttgat cgccgggaaa ttccgctgaa aaccttccct gtgctggcga aaacggaccc
     6961 gctggcggag taccagtcgg aggccgggcg gcagcggccg ggtgatgatg attttgcggg
     7021 gctgtactga tggcactgga gcgatacaac gtcagccacg cgaagcggca ggcacggaac
     7081 gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag aactgtgttc cacggcggag
     7141 ctggttgcgc aacggctggg gattgcggcg gtacagccgg tttaccgctt cctggacagc
     7201 ctggtggcga aagggctgct ggtccgggcg aaatacccgg ttgacggccg tcaggtcagc
     7261 gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg acgaggacga gccgctgacg
     7321 gacattattc cgtttcagcc gtcgcgggtg tcggcggcgc agctgccgca ccggctggcc
     7381 gtgcagtccc tgcgcctcgc catggaggcc cggggggcca ccggctggcg gtacctgcac
     7441 cgtattgccc tgaaagggat gaaggtgccg gacgcgctgg cggagctgga cggcagaacg
     7501 gtggcgtttg aggtggaacg caccgtgaag agccgcaggc gctatcagga ggtggtgtcc
     7561 gggtatctgt ttaaccgccg ggcgaacggc atcgatgaaa tctggtacat ttgcccggac
     7621 cgggcgacgc aggtcagggt gcaacgggct attctggcgg tggatgaaat cgtgaacccg
     7681 cagaccggcg aggcccgcaa aacggtggag ctggaccggg agcggctgtt tgcgtgcttt
     7741 aaattcatga tgatggactg agcttataca ttcactcaga gaggttttta tgacaaatcg
     7801 tgttgttgcc gtggggcgtg aagccctgtt gatggtcgct gtcatggccg ggacgctggt
     7861 cgttctggcc ggaaacctgc tgaaactgct ttggcgcgta ctgccggtgc ttctgctgct
     7921 ctacggggtt gtgcagtttc tggcgaggaa cccggagatc cgtgactggc tgtttctgca
     7981 tttcgggcag ggaggcacgg cggccattct tgtcggggga atactcctct gtatgcgccg
     8041 cctgatgaaa taatgccgga cagcaccggc tcggccggtg ctgttattcc tccccgagat
     8101 ggtcccgcat tgccagccag agcttttctg catcctcatg cagccgttcg cacatgtcgg
     8161 ccggggtatc catatccagc tcgttcagtt tctcgagcaa cagcagcgac tggtccttaa
     8221 tgaatttcgc catgttgagg gcggtttttt cctgcttcgt catgtttctg tccttccggg
     8281 cacggtctgc gtctgtcggg tatactgtct caaaaatagc ggggtgagac aatggcgtgg
     8341 gtgacggtga gacaggcgac ggaactgacg gggaaagccc ggagttccct gtatcgggat
     8401 atggcgaaag gccgcgtatc ataccgtacg gaagctgatg gtggtcgggt gatggatacc
     8461 agcgagctta tccgggtgta cggtgagtta agacagggtg agactcacga gcgggacggg
     8521 ttgagactgc cggatgagac tgaaaaaacg gccttcgacg cgttgatcgc ggagataaaa
     8581 gcactcaggg aggaggtggc cggactgcgg caggaaatgc aggccatgag actgctggaa
     8641 cacaaaccgg agacagcaga gacaccggcc ggaacaccgc gccggtggtg gcagtgggga
     8701 aaacgctgac gcgctgtgct tgtccaaccg gcgacggcca cgcaatgaat tgcgcccccg
     8761 tcaccggtta tcagcgaacg gtatggggat tctggcgctg g
//
