LOCUS       NZ_CP092277             5596 bp    DNA     circular CON 29-JUL-2023
DEFINITION  Klebsiella pneumoniae strain FJ8-TC plasmid p5, complete sequence.
ACCESSION   NZ_CP092277
VERSION     NZ_CP092277.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25889008
            Assembly: GCF_030544975.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Liu,C., Dong,N., Zeng,Y., Lu,J., Chen,J., Wang,Y., Chen,G., Shen,Z.,
            Chen,S. and Zhang,R.
  TITLE     Co-transfer of last-line antibiotic resistance and virulence operons
            by an IncFIBk-FII-X3-ColKP3 hybrid plasmid in Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Liu,C., Dong,N., Zeng,Y., Lu,J., Chen,J., Wang,Y., Chen,G., Shen,Z.,
            Chen,S. and Zhang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2022) Department of Clinical Laboratory, Second
            Affiliated Hospital, Zhejiang University School of Medicine,
            Department of Clinical Laboratory, Second Affiliated Hospital of
            Zhejiang University, School of Medi, Hangzhou 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 17:18:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,007
            CDSs (total)                      :: 5,885
            Genes (coding)                    :: 5,706
            CDSs (with protein)               :: 5,706
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 73 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 33 of 179
            Pseudo Genes (multiple problems)  :: 33 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092277.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FJ8-TC"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="China: Zhejiang"
                     /collection_date="2021"
     gene            complement(325..453)
                     /locus_tag="MHK76_RS29985"
     CDS             complement(325..453)
                     /locus_tag="MHK76_RS29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            616..840
                     /locus_tag="MHK76_RS29990"
                     /old_locus_tag="MHK76_29970"
     CDS             616..840
                     /locus_tag="MHK76_RS29990"
                     /old_locus_tag="MHK76_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            824..1090
                     /locus_tag="MHK76_RS29995"
                     /old_locus_tag="MHK76_29975"
     CDS             824..1090
                     /locus_tag="MHK76_RS29995"
                     /old_locus_tag="MHK76_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1160..1510)
                     /locus_tag="MHK76_RS30000"
                     /old_locus_tag="MHK76_29980"
     CDS             complement(1160..1510)
                     /locus_tag="MHK76_RS30000"
                     /old_locus_tag="MHK76_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1576..2121)
                     /locus_tag="MHK76_RS30005"
                     /old_locus_tag="MHK76_29985"
     CDS             complement(1576..2121)
                     /locus_tag="MHK76_RS30005"
                     /old_locus_tag="MHK76_29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2147..2473)
                     /locus_tag="MHK76_RS30010"
                     /old_locus_tag="MHK76_29990"
     CDS             complement(2147..2473)
                     /locus_tag="MHK76_RS30010"
                     /old_locus_tag="MHK76_29990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            3629..3805
                     /locus_tag="MHK76_RS30015"
                     /old_locus_tag="MHK76_29995"
     CDS             3629..3805
                     /locus_tag="MHK76_RS30015"
                     /old_locus_tag="MHK76_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3795..4139
                     /locus_tag="MHK76_RS30020"
                     /old_locus_tag="MHK76_30000"
     CDS             3795..4139
                     /locus_tag="MHK76_RS30020"
                     /old_locus_tag="MHK76_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4348..>4782
                     /locus_tag="MHK76_RS30025"
                     /old_locus_tag="MHK76_30005"
                     /pseudo=""
     CDS             4348..>4782
                     /locus_tag="MHK76_RS30025"
                     /old_locus_tag="MHK76_30005"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4817..5326
                     /locus_tag="MHK76_RS30030"
                     /old_locus_tag="MHK76_30010"
     CDS             4817..5326
                     /locus_tag="MHK76_RS30030"
                     /old_locus_tag="MHK76_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            5333..5545
                     /locus_tag="MHK76_RS30035"
                     /old_locus_tag="MHK76_30015"
     CDS             5333..5545
                     /locus_tag="MHK76_RS30035"
                     /old_locus_tag="MHK76_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3464..3543
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2881..3278)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4130..5595
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.796"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 agcacgaaaa ggaactgacg gaagcgcgtt cccggcagcg aagctatgac ggtccgtcgc
       61 tgggctgaaa tgcaggaaaa cagggttctc atcgggcatt tacgctgaaa ccggatgtca
      121 ttcacctggc tgcatggcta tgcagccagg taaaatttat ctgtcgcgaa cggggcggtt
      181 tatcgggtgg tttgttgccg gttttacctg actgccaccc cgttcgcggc gaacccgtcc
      241 ggggcggtgc gggcaacggg tgttatgttt aatcagcgtg aacggcagat acaaaaaagc
      301 cccggagggc ttttaactac tgacttattt ttttgaagat ttgctgggtt tcaatttgtc
      361 agtatttccc ggcgtttttt tattgtcacg ctggcgttta tcaggttccc ctgttgaatc
      421 ggcaattcgt ttcggacccg gcttaagacc cataatcgtt atcctttcaa tagttaagag
      481 gaaagccctc aagctagata tattgaccgc gttacggatt atcaagaggg gctaacaaga
      541 ttattttttg ttcttttagg ttgattgtgg tactgttgat atacatgtat agcaaatggt
      601 gttcgggagg tttatatgct tgctatccgg ttatctgatg agattgagtc ccgtctggac
      661 tcgctggcga agcaaaccgg cagaacaaag acgttttatg cgcgggaagc aatactggcg
      721 catctggaag acctggagga ttattatctt tcagcagaaa ctgctgcacg cgttcgccgt
      781 ggtgatgaag cagtgcattc gtctgaagac gtgaggaagt cacttggtct ggacgattaa
      841 ctattccgat cgggcgctca aatcgttacg caagatggac aaacagaacg cacgacggat
      901 tgtggatttt atgagtttac gcattgcagt tgctgccgat cctcgccagt cagggaagcc
      961 gctcaaaggt gagctgggcg agttctggcg ctatcgggtg ggagattatc gcgttctgtg
     1021 tgagatccga gatgacgagc ttgttatcct tgccgccacg attggacatc gccgcgaagt
     1081 ttacgactga agcccgctat gcgggctttt ttgatgccga caacaggtat tatggtaaat
     1141 actcgactaa ctacgtgttc tactgtttgt cggttttagt taaccagcag gttgtcttaa
     1201 cgataatgta gccgttattt ttaactaata aggtcatatt gttaacgtca ggcataataa
     1261 aagacattat ggcctcagag ttatcatcag aagcagctct ctgcattact acttcgattc
     1321 ccttttcttt gaatctataa gtatccagtt gcctattaaa cttagaaaga cctacaacca
     1381 tacttagttt gttatcttta acagtaagag atgcttcctg agtaccatta catcggtagt
     1441 aagcggtgta accgtcagca aaagatcgac cggggaatat aaatgctagg aacgcaatta
     1501 accaatacat agttgagaat cttggtatta cacatttcac tatcctctct ttcatttttc
     1561 gttcaccata ctttgttatc tgatttttaa tttttctatt ttataaacca agtctctacc
     1621 aaaaaggcga aaattatcct caaataataa taattgttta agaacatcca tgaaagcagc
     1681 tgcttcaccc tcagttatgg gagctactct atataacata taacctgagt taagggtttc
     1741 ttttatactt tcttcaatgc tcattataaa tattagtgga ttatcatttt tcttccccca
     1801 tgggtttcta attaatcctt cgatgtactc aggtcctttg cgtcctattg cctgcgcaat
     1861 attttttatt tcatctgcgt gagggctttg ttgtacagct ttgtttatgc ctgcttgagt
     1921 gattttcaga gactctacac cgtgtatgtt gtatgagata aaatgtaata aattaagatg
     1981 caatgcaatt aatccatttt tgtttacatc tgagtatttg gataaaaaac gtaatgaagt
     2041 gttaactctc ccttgaagcc taacaattgg gagtgctgcc ttgtatattt ccttctccgt
     2101 taaataccgt ttaaaaaaca taattctccg cctccgtata gcgtgcctat tttatttcgt
     2161 cgtaaaactt agctctgaaa tattccttta gaaccatcat gtcattaata attgaatcta
     2221 tctcatccat tttttcttgg tattctttta atggtgaagg gagcggtaac tcaatatttg
     2281 aaaagtgatt ggcgagatag ggatcaaggt ttctcatcca ttcaggcttt tcgccaatgc
     2341 gttcttcaac tgaaagtgaa cctgataatc tctggttaat aagatcactt agactcattt
     2401 tggttttatt cgaaagccgt tgtaaggatt gctttaatcc tatatctagc atgatatttg
     2461 ttctttctct cattaccccc ccttgctatt gcgttttttg ttgcgcacaa agctaccatt
     2521 gcgcattaag aaacgcaata aaaaaggagg gagggagtgg tttgtacatg atgtgcagtg
     2581 cgctgagggc atcagtggcg ggtgtttttc cgtgggatgg atggagctcc ggggataaca
     2641 gaactgacga tcatttaaag gatcttcttg agatcctttt tttctgcgcg taatctcttg
     2701 cactgtaaac gaaaaaaccg cctggggagg cggtttgatc gaaggttaag tcagttgggg
     2761 aactgcttaa ccgggtaact gggtatacga gaccgcagat accaaaactg ttctttcagt
     2821 gtagccgcag ttaggcaccc acttcaagac ctctcaatat ctctctcgta tatatccagt
     2881 taccaatggc tgctgccagt ggcgtttttg cgtgtccgtc cgggttggac tcaagatgat
     2941 agttaccgga aagggcgcag cagtcgggct gaacgggggg ttcgtgcata cagcccagct
     3001 tggagcgaac gacctaaacc gagccgagat accagcagcg tgagctatga gaaagcgcca
     3061 cgcttcccga aagggaaaaa ggcggaacag gtatccggta aacggcaggg tcggaacagg
     3121 agagcgcacg agggagccgc cggagagaaa tcaccggcat ctttaagtcc tgtcgggttt
     3181 cgtcctcctc tgacttgagc gtccatttct gtgatgctcg tcaggggagg cggagcctat
     3241 ggaaaaacgg caacgccgca tccttttctc cggccctttc tgatttgagc gcctttgctt
     3301 tatcccgtta tccgtgagtc ggtcacgccc gccgcagccg aacggcagga gcgcagctac
     3361 cgagtgagcg aggaggcgtc atttcatctg tccgtgcatg gtattgcgga cgctcctgag
     3421 ccacatatca cacctgccac atgaggcact ttcatgaatc gtgctctcat gccaacatag
     3481 taagccagta tacacagcgt aaacgctgtg actggttcag ggctgcgccc cgaaacccgc
     3541 taacaccact gacgcgctgg cgcttgtcca acgccggagc gacccggaaa acttttccgt
     3601 gccgtccggc gtttgcgaag gagggataat gcagaagccg aagaagaagc cgctcgtcgt
     3661 aaaaaaaaga tcctggcatc cggcagtgat ggcaaatctg atgcgtcagg cacaggagga
     3721 agattaccgc cgggagcaga tggcagagct tgagcgttca gcccggtcac attttgcgga
     3781 tatcggagga gaggatggcg gttaagcgta cccgtttttt aggtatccgg gtgaccgatg
     3841 gggagtacca gcagctgctg gagcgctgca atggaaggca actggcggtg tggatgcggg
     3901 aaacctgtct cgatacgcgc ccggcgcggt cgttgcggct gccgtccatc gatccggttt
     3961 tgctgcgcca gcttgccggg atggggaaca acctcaacca gatcgcccgg aaaattaacg
     4021 gcggccagtg gtcaggcgct gaccgggttc aggtggtggc cgcgctgatg gccattgatg
     4081 ccgaactcga gcggctgcgg catgccgtgc tggaaaaggg ggcagacgat gatcgttaag
     4141 tttcatccgc gagggcgagg cggcggtgcc gggccggtgg attatctgct ggggaaagac
     4201 cgccagcgcg acggggccat cgtcctgcag ggaaagccgg aggaggtccg ggagcttatc
     4261 gacgcctcgc cctacgccaa aaagtacacc tccggcgtcc tgtcttttgc tgaacaggat
     4321 ttaccgcccg gccagcgtga aaagctgatg gcgagcttcg agcgggttct gatgcccgga
     4381 ctcgataaag accagtacag cgtgctgtgg gttgaacacc gggacaaggg gcggctggaa
     4441 ctgaattttc tggtaccgaa cacggaactg ctgaccggca ggcgtctcca gccgtactac
     4501 gaccgggcag accgtccgcg catcgatgcg tggcagaccg tggtgaacgg gcgactgggg
     4561 ctgcacgacc cgaacgcacc ggagaaccgg cgcgcgctgg tgacgccatc ttcgttaccc
     4621 aaaacgaagc aggaagccgc agaggctatt acgcggggct tactggccct cgcctcgtca
     4681 ggggagctta aaacgcgtca ggacgtcact gaggcgctgg aaagcgcagg ttttgaggtc
     4741 gtgcgcacca caaagagcag catcagcatt gccgacccgg acggagggcg aaacatccga
     4801 cttaagggag ccatctatga acagtctttt aacgctggcg aaggacttag agcagaaatc
     4861 gaaagcgcag cagcagagta ccggcgaaat gctgaaagcc gcattcagcg agcacgagaa
     4921 gtctgtcaga gcggaactga gcgaaagcgg gaagagaatc agcgccgcca tccgcgccca
     4981 cgagcagggt atgagcgcgg ccatgcagtc gaaccgcctg agcgtgatgc gtatggtcgg
     5041 gcggacatgg ctgaccatca cgatggtgtc aggactgctg ttcgccagcc tgagcggggt
     5101 gttgtggtat caggggagct tgatagcgtc aaatctggcc gaaatcgacc ggcagaacgc
     5161 agcgctgtcg aagctgaacg cgaagacctg gggcgtgacg tacctggaag acagcaacgg
     5221 acgttttctg gtgctgccga aggggacggc cgtagacagg acgcagagct ggacggtcgg
     5281 gaacgggcgg tcgaagcaga acgcgctgag actggtgaag gagtaagtgc cgatgaccga
     5341 gctggaaaaa cagttgctga gcgcattaga gcagctacag caggactact cgaaaaggct
     5401 ggacgagtgg gagagcgcct tcggggaatg gcggaaaatg tcagggctta tgcaacggga
     5461 gaacgcggcg ctgagcgagc gcgtgacgcg cttgagtcag caggtggaac gcttgagcgg
     5521 gcaactgcag cgtttgagcc ggtagtgcag cgtcatgaga tggccgtggc cgcagaacgg
     5581 gcgcacgagc aacggc
//
