LOCUS       NZ_CP092257             4499 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Escherichia coli strain ISHS7 plasmid pISHS7_5, complete sequence.
ACCESSION   NZ_CP092257
VERSION     NZ_CP092257.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25898031
            Assembly: GCF_022349925.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Sung,I.S., Lai,G.K., Griffin,S.D. and Leung,F.C.
  TITLE     Complete genome sequence of Escherichia coli ISHS7, isolated from
            faeces of a domestic cat (Felix catus domesticus)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Sung,I.S., Lai,G.K., Griffin,S.D. and Leung,F.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2022) Shuyuan Molecular Biology Laboratory, The
            Independent Schools Foundation Academy, 1 Kong Sin Wan Road,
            Pokfulam, Hong Kong HK SAR, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Assembly Name                     :: Irwin_Ecoli_ISHS7cat
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 70.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022349925.1-RS_2024_05_08
            Annotation Date                   :: 05/08/2024 06:16:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,916
            CDSs (total)                      :: 4,798
            Genes (coding)                    :: 4,625
            CDSs (with protein)               :: 4,625
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 73 of 173
            Pseudo Genes (incomplete)         :: 103 of 173
            Pseudo Genes (internal stop)      :: 35 of 173
            Pseudo Genes (multiple problems)  :: 37 of 173
            Pseudo Genes (short protein)      :: 2 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092257.1.
            Bacteria and source DNA available from Dr. Simon D. J. Griffin,
            Shuyuan Molecular Biology Laboratory, ISF Academy, 1 Kong Sin Road,
            Pokfulam, Hong Kong.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ISHS7"
                     /isolation_source="feces"
                     /host="Felix catus domesticus"
                     /db_xref="taxon:562"
                     /plasmid="pISHS7_5"
                     /geo_loc_name="Hong Kong: North Point"
                     /lat_lon="22.285459 N 114.201753 E"
                     /collection_date="2020-06-18"
                     /collected_by="Irwin S. H. Sung"
     gene            join(4155..4499,1..684)
                     /locus_tag="MF614_RS24435"
                     /old_locus_tag="MF614_24435"
     CDS             join(4155..4499,1..684)
                     /locus_tag="MF614_RS24435"
                     /old_locus_tag="MF614_24435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="WP_096951530.1"
                     /translation="MGDFFKRNDIDEEIEKESIGYWIQDLQKSSKLLMIIKTAAFNRLY
                     GISFLGAIDYSDQNELTKKERNRAIHSLYVAGIANYVATKRNYNSDLKEHLVAAALLHD
                     IGHMPLSHSAEPFIKEKFGYGHHELGNNLISGTCWVDSGLSKALNKNFDVSFIKGLLNF
                     ELKNDGHDIFSNKINADTIDGIVRCIEYKGKNKANHLNRISIAKAAFLDNIDMTENKRL
                     QILDNFWNTKHFVYQNFINTKHSVISDKISQIFFRENKKIEYKNLLSDERRWREQYKLL
                     FKWLSNLKSNSIPHCLKNYEMIYTTRNYKVLKTEKDIKKRYTNNKNKAKISEDSLLKYR
                     EE"
                     /name="HD domain-containing protein"
     gene            711..1706
                     /locus_tag="MF614_RS24440"
                     /old_locus_tag="MF614_24440"
     CDS             711..1706
                     /locus_tag="MF614_RS24440"
                     /old_locus_tag="MF614_24440"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /protein_id="WP_115435198.1"
                     /translation="MSYTEIVKKYENISKSTSYSVDILGKLEDDLQKIFSEKNLEFTIV
                     TTGSFGRLEASEESDIDLFIFCKKESEKHYILYKKEEIETCIKKYIHKKAGDTGTFGVD
                     AVDVFDNILKNIGGGDDTNKSLTRRMLFLLEGKAIYNKNIFNSYKKTLITKYLNSSSEG
                     KLDKYLLNDVIRYYRTITTDFQHKVDAGGKSWGIRNIKLRYSRKLLYFAGVMAIAHASE
                     TQQDITKRTQEISNSLDIPPLERIYNISHGKAKPELIEDIFCQYEFFLSCISDPEKRAS
                     LNDIKDKSDRLNSPLYNQLSEKSTEFTDNLYKLLNNVFEKNNQIHTSLIF"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            3025..3222
                     /locus_tag="MF614_RS24445"
                     /old_locus_tag="MF614_24445"
     CDS             3025..3222
                     /locus_tag="MF614_RS24445"
                     /old_locus_tag="MF614_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_096951528.1"
                     /translation="MNRQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELT
                     EELQNSIQTHFEAQYGTEQP"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          3353..3838
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.165"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ccgtttataa aagaaaaatt tggttatggc catcatgagc tcggaaataa tttaatatct
       61 ggcacatgct gggtagacag cggtttaagc aaggctctaa acaaaaactt tgacgttagt
      121 tttataaaag gcctgttaaa tttcgaacta aaaaatgatg gacacgacat cttctcaaat
      181 aaaataaatg ccgatactat cgatggcata gtgaggtgca tagagtacaa aggaaagaat
      241 aaagcaaacc atttaaatag aatttctatt gcaaaagctg cattcttaga taacatagat
      301 atgacagaga ataaaaggct gcagatatta gataattttt ggaatacaaa gcattttgta
      361 tatcaaaatt ttatcaatac aaaacatagc gtcatatcag acaagataag tcagattttt
      421 ttcagggaaa ataaaaagat agaatacaaa aacttactct cagacgaaag acgctggaga
      481 gaacaatata aattgctgtt taaatggcta tcaaacctaa aatcaaactc aatacctcat
      541 tgcctaaaaa attacgagat gatatacaca acaagaaatt ataaagtgtt aaagacagaa
      601 aaagacatta aaaaaagata cacaaacaat aaaaataaag caaagataag tgaagatagt
      661 cttttaaaat acagagaaga atgatatcag taagagtgta gggataaaaa atgagctata
      721 ctgaaatagt caaaaaatac gaaaacattt caaaatcaac gagttatagc gttgacattc
      781 ttggtaaatt agaggatgat ctacaaaaga tattttcaga aaaaaattta gagttcacca
      841 tagtcactac aggatctttc ggtagactag aagcgtcaga agaatctgat atagatctct
      901 ttattttctg taaaaaggaa tcggaaaaac actacattct atataaaaaa gaagaaatag
      961 agacttgcat aaaaaaatac attcataaaa aagccggtga tacaggtacg tttggcgttg
     1021 atgcggtaga tgtatttgat aatattttaa aaaacattgg tggtggggat gatacaaaca
     1081 aatctctaac cagaaggatg ttgtttctac tagaagggaa ggcaatatat aacaaaaata
     1141 ttttcaatag ctacaagaaa acattaatta ccaaatattt gaactcctca agcgaaggaa
     1201 agctcgacaa atatttgctt aacgatgtta taagatatta taggactatc actactgact
     1261 ttcagcataa agttgatgcc ggtggcaagt cttggggtat ccgcaatata aaactacgct
     1321 attcccgtaa gctgctttat tttgcaggcg taatggctat cgctcatgcc agtgaaacac
     1381 aacaagacat aacaaaacga acacaagaaa tatctaatag tttagatata ccacctttag
     1441 agagaatata taatatcagc cacggcaaag caaaacctga actgatagaa gacatcttct
     1501 gtcaatatga gttcttctta agctgcatca gtgacccaga gaaaagagct agtttaaacg
     1561 acataaaaga taaatcagat agattaaact cccctcttta caatcaactg agcgaaaaaa
     1621 gcacagaatt cacagataat ttatataaat tgcttaacaa tgtgtttgaa aagaacaatc
     1681 aaatacatac ctcattaatt ttttaactta aaggccgctt tcaagcggcc ttttttgcat
     1741 ttaaatcctc aacacaagcc aatttaccat aacatcagtt tgtacgcacc gctgaaacgc
     1801 gctcagcgcg attctgacgc aaaaaccgta aaaagggatg ttttccccag ggtaaaccgg
     1861 gaaaatgcgt caggaacgtt ttcagcgcgt tgcatgacta tgcatgaaac tgaatggcga
     1921 tcggtttggg cgcgcccgct gtccctgagg cttattttcg tcaattttca gttcgtaatt
     1981 ctctgactca ttgctgcata tcagaccaga gcgatcctag gggcgcccct aggatcgctc
     2041 tgtaaaaaaa tgcgacacac ccctagggcc gcccctacat taattaactg ttttttctac
     2101 agatagtcag tactgaccct aggtagggtc gtcctagggt cgccgacgac gcgcttttgt
     2161 ataaatctga ataagaaaat acatttttta aaacataaca acctgattta aaaaagaaat
     2221 cagcaaaaag ttagttttgc gtggggtgtg ggcatccggg gcatgagaac agactcgcgt
     2281 ttttctggag gaacagcggg gatttttgat taaacagtac tcaccgcaga gcggaatttt
     2341 atgcagcgcc agctgtgctg cacggggatt tttaatcccc tgccccgttg agccgctcca
     2401 cggcgaataa gggaaagtaa agttagctaa ctttacatgt cctgcccggt gttcactggt
     2461 gtttatggcg gggaacagaa ccaccggaca ggtggaaaca tgtgtgtttc tgtgtgccct
     2521 gtgtaatccg gtggatttgt gtgtaaaggg ttgattcgct ccgcagttcg tctgaggtgt
     2581 cggggctgtg ccctgaccaa gcgttttgct gaacggccat caccgatggc ggttcaacaa
     2641 aacatatcct gtcccgcctt tgcgttcgcc gttcgcgcat cgctggccat cactccgggt
     2701 cactttgtca gatgttaaaa aatcacgcgg taaacgccgt tatgcgctgg tttgcacatg
     2761 catctcccgt gcgtcacttt gtcaaaacgt caaagtgacg cacatcagga accgccggaa
     2821 cgttcatgtt tatgacgaca acatgcacac aacgcagaca gaaaatcgcg cacatattca
     2881 gcaaaaaata agcgtcacgg ggtgttaatt tcgcgcagcc cacacgcgac ggggcttgtg
     2941 tgataatttt cagtgacgtc acctggcgta atgaccaggt gacgcacagc cggacgtaaa
     3001 caccagtaac ggagacggga gaacgtgaac agacagcagc agaccgcgct caacatggcg
     3061 cgattcatca aaagccagag cctgacgttg ctcgagaaac tggacgcact ggatgccgac
     3121 gaacaggccg ccatgtgcga acgactgcac gaactgacgg aagaactgca gaacagcata
     3181 cagactcact ttgaagctca gtacggcaca gaacaaccat aaaggcgctg gcgaaagcgg
     3241 tcgcggcttg tccgtgagcg ttttcgccag cagacaagcc cggagggcgt gtcagagcat
     3301 gagcggaagg aacacaggcg cgtaagtgct caaatcagac tggcggtgaa attccgcttc
     3361 ctcgcccact cgctcactac gttcggtcgc tggggctgcg gcgagcggtg gaggctctca
     3421 cataacaacg gacagtttat ccacagaatc gggggataac caggacagaa catgtgagca
     3481 aagagcacga aaaaaagcgc accggtggcg tttttccata ggctccgccc ccctgacgag
     3541 catcacaaaa accgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3721 tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3841 catgcaaaag caccattggc agcagccatt ggtaactgat ttagaggagt tagtcttgat
     3901 gttatgcgcc ggttaaggct aaactgaaag aacaagtttt ggtgactgcg ctcctctacc
     3961 agccagttac ctcggttcaa aagagcgcca actctagaga acctttgaaa aaccgcccgc
     4021 caaggcggtt ttttcatttt caacgaggta aacagtatct tgacatggca cgaatcgtca
     4081 actggattgt gtgagcaagt tgtcatatta agtgttagag tcaatttaac gtgaatgatt
     4141 tgagagcaga tattatgggt gatttcttca aaagaaatga cattgatgag gagattgaaa
     4201 aagaaagcat tggatactgg attcaagact tacaaaaatc cagtaaatta ttaatgataa
     4261 taaaaactgc cgcatttaat cgcttatacg gaatatcatt cttaggtgct atcgattata
     4321 gcgatcaaaa cgaactcaca aaaaaagaaa gaaacagagc aattcactct ttatatgtcg
     4381 caggaatagc aaactatgta gcaactaaaa ggaattacaa ttcggatctc aaagagcatt
     4441 tagtagccgc agctttactg catgacatag gacatatgcc gttatcacat agcgcagag
//
