LOCUS       NZ_CP092122             3585 bp    DNA     linear   CON 15-APR-2024
DEFINITION  Klebsiella quasipneumoniae subsp. similipneumoniae strain IF3SW-P1
            plasmid pECQ4552_IHU08, complete sequence.
ACCESSION   NZ_CP092122
VERSION     NZ_CP092122.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25845549
            Assembly: GCF_022313415.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. similipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. similipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Sushenko,N.S., Singh,N.K., Vellone,D.L., Tighe,S.W., Hedlund,B.P.,
            Venkateswaran,K. and Moser,D.P.
  TITLE     Complete Genome Sequence of Klebsiella quasipneumoniae subsp.
            similipneumoniae Strain IF3SW-P1, Isolated from the International
            Space Station
  JOURNAL   Microbiol Resour Announc 11 (7), e0047622 (2022)
   PUBMED   35735981
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Sushenko,N.S., Singh,N.K., Vellone,D.L., Tighe,S.W., Hedlund,B.P.,
            Venkateswaran,K. and Moser,D.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2022) Department of Hydrological Sciences, Desert
            Research Institute, 755 E Flamingo Rd, Las Vegas, NV 89119, USA
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 3585)
  AUTHORS   Sushenko,N.S., Singh,N.K., Vellone,D.L., Tighe,S.W., Hedlund,B.P.,
            Venkateswaran,K. and Moser,D.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2022) Department of Hydrological Sciences, Desert
            Research Institute, 755 E Flamingo Rd, Las Vegas, NV 89119, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-DEC-2021
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022313415.2-RS_2024_04_15
            Annotation Date                   :: 04/15/2024 05:27:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,045
            CDSs (total)                      :: 4,923
            Genes (coding)                    :: 4,827
            CDSs (with protein)               :: 4,827
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 61 of 96
            Pseudo Genes (incomplete)         :: 29 of 96
            Pseudo Genes (internal stop)      :: 18 of 96
            Pseudo Genes (multiple problems)  :: 11 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092122.1.
            Bacteria and source DNA available from Kasthuri Venkateswaran.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Klebsiella quasipneumoniae subsp.
                     similipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="IF3SW-P1"
                     /isolation_source="Foot panel of ARED on ISS"
                     /sub_species="similipneumoniae"
                     /db_xref="taxon:1463164"
                     /plasmid="pECQ4552_IHU08"
                     /collection_date="2015-03-04"
                     /collected_by="Terry Virts"
     gene            234..428
                     /locus_tag="MEO32_RS25180"
                     /old_locus_tag="MEO32_25175"
     CDS             234..428
                     /locus_tag="MEO32_RS25180"
                     /old_locus_tag="MEO32_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="WP_001421937.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEAC
                     RRRLTSEKRAYTSIKSDEE"
                     /name="YlcI/YnfO family protein"
     gene            complement(593..799)
                     /gene="ybcW"
                     /locus_tag="MEO32_RS25185"
                     /old_locus_tag="MEO32_25180"
     CDS             complement(593..799)
                     /gene="ybcW"
                     /locus_tag="MEO32_RS25185"
                     /old_locus_tag="MEO32_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="WP_001031427.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLEA
                     FRAIEAALVKHDNNMKDYSLVVD"
                     /name="ybcW"
     gene            1085..1495
                     /locus_tag="MEO32_RS25190"
                     /old_locus_tag="MEO32_25185"
     CDS             1085..1495
                     /locus_tag="MEO32_RS25190"
                     /old_locus_tag="MEO32_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="WP_012775990.1"
                     /translation="MAQVAIFKEIFDQVRKDLDCELFYSELKRHNVSHYIYYLATDNIH
                     IVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFRWV
                     TNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
                     /name="DUF1398 domain-containing protein"
     gene            1785..2078
                     /gene="bor"
                     /locus_tag="MEO32_RS25195"
                     /old_locus_tag="MEO32_25190"
     CDS             1785..2078
                     /gene="bor"
                     /locus_tag="MEO32_RS25195"
                     /old_locus_tag="MEO32_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="WP_000738492.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVAPKETITHHFFVSG
                     IGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
                     /name="bor"
     gene            complement(2110..2571)
                     /gene="rzpD"
                     /locus_tag="MEO32_RS25200"
                     /old_locus_tag="MEO32_25195"
     CDS             complement(2110..2571)
                     /gene="rzpD"
                     /locus_tag="MEO32_RS25200"
                     /old_locus_tag="MEO32_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage endopeptidase RzpD"
                     /protein_id="WP_000092275.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKAQRDKNARELK
                     LANAAITDMQMRQRDVAALDAKYTKELADAKAENDALRDDVAAGRRRLHIKAVCQSVRE
                     ATTASGVDNAASPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
                     /name="rzpD"
     gene            complement(2568..3044)
                     /locus_tag="MEO32_RS25205"
                     /old_locus_tag="MEO32_25200"
     CDS             complement(2568..3044)
                     /locus_tag="MEO32_RS25205"
                     /old_locus_tag="MEO32_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /GO_function="GO:0003796 - lysozyme activity [Evidence
                     IEA]"
                     /GO_process="GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]; GO:0009253 - peptidoglycan
                     catabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="WP_000229403.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTDY
                     SDHPRKLVTLNPKLKSTGAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKERGA
                     LPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
                     /name="glycoside hydrolase family protein"
     gene            complement(3028..3351)
                     /locus_tag="MEO32_RS25210"
                     /old_locus_tag="MEO32_25205"
                     /pseudo=""
     CDS             complement(3028..3351)
                     /locus_tag="MEO32_RS25210"
                     /old_locus_tag="MEO32_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /name="phage holin, lambda family"
ORIGIN
        1 gcttacggtt cactactcac gacgatgttt tttttggtac cttttttttc accggaaagg
       61 acccgtaaag tgataatgat tatcatctac atatcacaac gtgcgtggag gccatcaaac
      121 cacgtcaaat aatcaattat gacgcaggta tcgtattaat tgatctgcat caacttaacg
      181 taaaaacaac ttcagacaat acaaatcagc gacactgaat acggggcaac ctcatgtcaa
      241 cgaagaacag aacccgcaga acaacaaccc gcaacatccg ctttcctaac caaatgattg
      301 aacaaattaa catcgctctt gagcaaaaag ggtccgggaa tttctcagcc tgggtcattg
      361 aagcctgccg tcggagacta acgtcagaaa agagagcata tacatcaatt aaaagtgatg
      421 aagaatgaac atcccgcgtt cttccctccg aacaggacga tattgtaaat tcacttaatt
      481 acgagggcat tgcagtaatt gagttgcagt tttaccactt tcctgacagt gacagactgc
      541 gtgttggctc tgtcacagac taaatagttt gaatgattag cagttatggt gatcagtcaa
      601 ccaccaggga ataatccttc atattattat cgtgcttcac caacgctgcc tcaattgctc
      661 tgaatgcttc cagagacacc ttatgttcta tacatgcaat tacaacatca gggtaactca
      721 tagaaatggt gctattaagc atatttttta cacgaatcag atccacggag ggatcatcag
      781 cagattgttc tttattcatt ttgtcgctcc atgcgcttgc tcttcatcta gcggttaaaa
      841 tattacttca aatctttctg tatgaagatt tgagcacgtt ggccttacat acatctgtcg
      901 gttgtatttc cctccagaat gccagcagga ccgcactttg ttacgcaacc aatactatta
      961 agtgaaaaca ttcctaatat ttgacataaa tcatcaacaa aacacaagga ggtcagacca
     1021 gattgaaacg ataaaaacga taatgcaaac tacgcgccct cgtatcacat ggaaggtttt
     1081 accaatggct caggttgcca tttttaaaga aatattcgat caagtgcgaa aagatttaga
     1141 ctgtgaattg ttttattctg aactaaaacg tcacaacgtc tcacattata tttactatct
     1201 agccacagat aatattcaca tcgtgttaga aaacgataac accgtgttaa taaaaggact
     1261 taaaaaggtt gtaaatgtta aattctcaag aaacacgcat cttatagaaa cgtcctatga
     1321 taggttgaaa tcaagagaaa tcacatttca gcaatacagg gaaaatcttg ctaaagcagg
     1381 agttttccga tgggttacaa atatccatga acataaaaga tattactata cctttgataa
     1441 ttcattacta tttactgaga gcattcagaa cactacacaa atctttccac gctaaatcat
     1501 aacgtccggt ttcttccgtg tcagcaccgg ggcgttggca taatgcaata cgtgtacgcg
     1561 ctaaaccctg tgtgcatcgt tttaattatt cccggacact cccgcagaga agttccccgt
     1621 cagggctgtg gacatagtta atccgggaat acaatgacga ttcatcgcac ctgacataca
     1681 ttaataaata ttaacaatat gaaatttcaa ctcattgttt agggtttgtt taattttcta
     1741 cacatacgat tctgcgaact tcaaaaagca tcgggaataa caccatgaaa aaaatgctac
     1801 tcgctactgc gctggccctg cttattacag gatgtgctca acagacgttt actgttcaaa
     1861 acaaaccggc agcagtagca ccaaaggaaa ccatcaccca tcatttcttc gtttctggaa
     1921 ttgggcagaa gaaaactgtc gatgcagcca aaatttgtgg cggcgcagaa aatgttgtta
     1981 aaacagaaac ccagcaaaca ttcgtaaatg gattgctcgg ttttattact ttaggcattt
     2041 atactccgct ggaagcgcgt gtgtattgct cacaataatt gcatgagttg cccatcgata
     2101 tgggcaactc tatctgcact gctcattaat atacttctgg gttccttcca gttgtttttg
     2161 catagtgatc agcctctctc tgagggtgaa ataatcccgt tcagcggtgt ctgccagtcg
     2221 gggggaggct gcattatcca cgccggaggc ggtggtggct tcacgcactg actgacagac
     2281 tgctttgatg tgcaaccgac gacgaccagc ggcaacatca tcacgcagag catcattttc
     2341 agctttagca tcagctaact ccttcgtgta ttttgcatcg agcgcagcaa catcacgctg
     2401 acgcatctgc atgtcagtaa ttgccgcgtt cgccagcttc agttctctgg catttttgtc
     2461 gcgctgggct ttgtaggtaa tggcgttatc acggtaatga ttaacagccc atgacaggca
     2521 gacgatgatg cagataacca gagcggagat aatcgcggtg actctgctca tacatcaatc
     2581 tctctgaccg ttccgcccgc ttctttgaat tttgcaatca ggctgtcagc cttatgctcg
     2641 aactgaccat aaccagcgcc cggcagtgaa gcccagatat tgctgcaacg gtcgattgcc
     2701 tgacggatat caccacgatc aatcataggt aaagcgccac gctccttaat ctgctgcaat
     2761 gccacagcgt cctgactttt cggagagaag tctttcaggc caagctgctt gcggtaggca
     2821 tcccaccaac gggaaagaag ctggtagcgt ccggcgcctg ttgatttgag ttttgggttt
     2881 agcgtgacaa gtttgcgagg gtgatcggag taatcagtaa atagctctcc gcctacaatg
     2941 acgtcataac catgatttct ggttttctga cgtccgttat cagttccctc cgaccacgcc
     3001 agcatatcga ggaacgcctt acgttgatta ttgatttcta ccatcttcta ctccggcttt
     3061 tttagcagcg aagcgtttga taagcgaacc aatcgagtca gtaccgatgt agccgataaa
     3121 cacgctcgtt atataagcga gattgctact tagtccggcg aagtcgagaa ggtcacgaat
     3181 gaactaggcg ataatggcgc acatcgttgc gtcgattact gtttttgtaa acgcaccgcc
     3241 attatatctg ccgcgaaggt acgccattgc aaacgcaagg attgccccga tgccttgttc
     3301 ctttgccgcg agaatggcgg ccaacaggtc atgtttttct ggcatcttca tgtcttaccc
     3361 ccaataaggg gatttgctct atttaattag gaataaggtc gattactgat agaacaaatc
     3421 caggctactg tgtttagtaa tcagatttgt tcgtgaccga tatgcacggg caaaacggca
     3481 ggaggttgtt agcgcaaaaa aaaaattcca aaaaaaaaat tccaaaaaaa aaagcgacta
     3541 acaaacacaa tctgatggca gcaatacgta actgaacgaa gtaca
//
