LOCUS       NZ_CP092073            20644 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Staphylococcus argenteus strain 1710042798 plasmid p1_1710042798,
            complete sequence.
ACCESSION   NZ_CP092073
VERSION     NZ_CP092073.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25742062
            Assembly: GCF_022226895.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus argenteus
  ORGANISM  Staphylococcus argenteus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20644)
  AUTHORS   Shittu,A.O., Layer-Nicolaou,F., Strommenger,B., Nguyen,M.-T.,
            Bletz,S., Mellmann,A. and Schaumburg,F.
  TITLE     First report of a methicillin-resistant, high-level
            mupirocin-resistant Staphylococcus argenteus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20644)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022226895.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 08:10:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,678
            CDSs (total)                      :: 2,596
            Genes (coding)                    :: 2,522
            CDSs (with protein)               :: 2,522
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 38 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 24 of 74
            Pseudo Genes (multiple problems)  :: 15 of 74
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092073.1.
            Bacteria and source DNA available from Frieder Schaumburg, Inst.
            Med. Microbiology, University Hospital Muenster, Muenster, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20644
                     /organism="Staphylococcus argenteus"
                     /mol_type="genomic DNA"
                     /strain="1710042798"
                     /host="Homo sapiens"
                     /db_xref="taxon:985002"
                     /plasmid="p1_1710042798"
                     /geo_loc_name="Germany"
                     /collection_date="2021"
                     /collected_by="Inst. Med. Microbiology, Muenster, Germany"
     gene            join(20324..20644,1..540)
                     /locus_tag="MCF40_RS13255"
                     /old_locus_tag="MCF40_13255"
     CDS             join(20324..20644,1..540)
                     /locus_tag="MCF40_RS13255"
                     /old_locus_tag="MCF40_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_031788340.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERENFLKQL
                     ELNWE"
                     /name="RepB family plasmid replication initiator protein"
     gene            627..968
                     /locus_tag="MCF40_RS13260"
                     /old_locus_tag="MCF40_13260"
     CDS             627..968
                     /locus_tag="MCF40_RS13260"
                     /old_locus_tag="MCF40_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000761341.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(1207..1278)
                     /locus_tag="MCF40_RS13265"
                     /old_locus_tag="MCF40_13265"
     CDS             complement(1207..1278)
                     /locus_tag="MCF40_RS13265"
                     /old_locus_tag="MCF40_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106873653.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            1427..1612
                     /locus_tag="MCF40_RS13270"
                     /old_locus_tag="MCF40_13270"
     CDS             1427..1612
                     /locus_tag="MCF40_RS13270"
                     /old_locus_tag="MCF40_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466619.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(1831..2574)
                     /locus_tag="MCF40_RS13275"
                     /old_locus_tag="MCF40_13275"
     CDS             complement(1831..2574)
                     /locus_tag="MCF40_RS13275"
                     /old_locus_tag="MCF40_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843757.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEAKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            2985..3661
                     /locus_tag="MCF40_RS13280"
                     /old_locus_tag="MCF40_13280"
                     /pseudo=""
     CDS             2985..3661
                     /locus_tag="MCF40_RS13280"
                     /old_locus_tag="MCF40_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            <3672..3954
                     /locus_tag="MCF40_RS13285"
                     /old_locus_tag="MCF40_13285"
                     /pseudo=""
     CDS             <3672..3954
                     /locus_tag="MCF40_RS13285"
                     /old_locus_tag="MCF40_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            5163..5780
                     /gene="cadD"
                     /locus_tag="MCF40_RS13290"
                     /old_locus_tag="MCF40_13290"
     ARG             5164..5777
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            5799..6146
                     /locus_tag="MCF40_RS13295"
                     /old_locus_tag="MCF40_13295"
     CDS             5799..6146
                     /locus_tag="MCF40_RS13295"
                     /old_locus_tag="MCF40_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119691.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            6306..6398
                     /locus_tag="MCF40_RS13300"
                     /old_locus_tag="MCF40_13300"
     CDS             6306..6398
                     /locus_tag="MCF40_RS13300"
                     /old_locus_tag="MCF40_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            6610..7584
                     /locus_tag="MCF40_RS13305"
                     /old_locus_tag="MCF40_13305"
     CDS             6610..7584
                     /locus_tag="MCF40_RS13305"
                     /old_locus_tag="MCF40_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WP_000072390.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKATLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(7704..8519)
                     /locus_tag="MCF40_RS13310"
                     /old_locus_tag="MCF40_13310"
     CDS             complement(7704..8519)
                     /locus_tag="MCF40_RS13310"
                     /old_locus_tag="MCF40_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000164958.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(8512..9955)
                     /locus_tag="MCF40_RS13315"
                     /old_locus_tag="MCF40_13315"
                     /pseudo=""
     CDS             complement(8512..9955)
                     /locus_tag="MCF40_RS13315"
                     /old_locus_tag="MCF40_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /name="Mu transposase C-terminal domain-containing protein"
     gene            complement(9927..10520)
                     /locus_tag="MCF40_RS13320"
                     /old_locus_tag="MCF40_13320"
     CDS             complement(9927..10520)
                     /locus_tag="MCF40_RS13320"
                     /old_locus_tag="MCF40_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690638.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(10784..11164)
                     /gene="blaI"
                     /locus_tag="MCF40_RS13325"
                     /old_locus_tag="MCF40_13325"
     ARG             complement(10788..11164)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(11154..12911)
                     /gene="blaR1"
                     /locus_tag="MCF40_RS13330"
                     /old_locus_tag="MCF40_13330"
     ARG             complement(11158..12911)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            13018..13863
                     /gene="blaZ"
                     /locus_tag="MCF40_RS13335"
                     /old_locus_tag="MCF40_13335"
     ARG             13019..13860
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            14232..14765
                     /locus_tag="MCF40_RS13340"
                     /old_locus_tag="MCF40_13340"
     CDS             14232..14765
                     /locus_tag="MCF40_RS13340"
                     /old_locus_tag="MCF40_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823239.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001823239.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNESK
                     RRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEVNIT
                     RQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            15179..15472
                     /locus_tag="MCF40_RS13345"
                     /old_locus_tag="MCF40_13345"
     CDS             15179..15472
                     /locus_tag="MCF40_RS13345"
                     /old_locus_tag="MCF40_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000726502.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            15547..17481
                     /locus_tag="MCF40_RS13350"
                     /old_locus_tag="MCF40_13350"
     CDS             15547..17481
                     /locus_tag="MCF40_RS13350"
                     /old_locus_tag="MCF40_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000713729.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            17485..17796
                     /locus_tag="MCF40_RS13355"
                     /old_locus_tag="MCF40_13355"
     CDS             17485..17796
                     /locus_tag="MCF40_RS13355"
                     /old_locus_tag="MCF40_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WP_000831610.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            17808..18434
                     /locus_tag="MCF40_RS13360"
                     /old_locus_tag="MCF40_13360"
     CDS             17808..18434
                     /locus_tag="MCF40_RS13360"
                     /old_locus_tag="MCF40_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000593103.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            18672..19301
                     /gene="mobV"
                     /locus_tag="MCF40_RS13365"
                     /old_locus_tag="MCF40_13365"
                     /pseudo=""
     CDS             18672..19301
                     /gene="mobV"
                     /locus_tag="MCF40_RS13365"
                     /old_locus_tag="MCF40_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            19395..19940
                     /locus_tag="MCF40_RS13370"
                     /old_locus_tag="MCF40_13370"
     CDS             19395..19940
                     /locus_tag="MCF40_RS13370"
                     /old_locus_tag="MCF40_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031788368.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSDNEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="hypothetical protein"
     ARG             13019..13863
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             10179..20472
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4012..4159
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          complement(1832..2574)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          20325..20644
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1e-166"
     Typing          18673..18947
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 ctagaaatat caacttcttc aaatttaaaa cacatattga attcaattac taataatttt
       61 acaaaatttg aattaaaaga aatgactagg cttaaatcta catattctaa aaatatgttt
      121 agattactta aacaatacaa acatacaggc tatttaaaaa ttcacatcga tgattttaaa
      181 aatcgtttag atatacctaa atcttatcgt atgactgata ttaacaaaaa tgtttttaaa
      241 cctattatta ttgaattagg ttctatattt aataatctta ctatcaataa aatcaaagcg
      301 aaaaaagggc gtaaaattga atggatagag ttcacatttg acgctgagaa acgcattcat
      361 aacaaacgac aacctcaaat gtccaagata gataaatcaa gacaatatgt gaggcgtgaa
      421 aagacgccta aatggttaga agaacggtca tatgaaaagc aacctcaaaa agattacgac
      481 ccacaattag agaaagaacg agagaatttt ttaaaacaac ttgaactgaa ttgggaataa
      541 cgcaactttt cgttgcgagt tggttaacta aaagtatcgt gacttttcaa ttattttaat
      601 tatagtttga gttaaggagt gattttatga aattagctga agctataaaa gaacagagag
      661 aattaaaagg atggtctcaa gaggaattag ccaatatatt aaaggtttcc agacaaagtg
      721 tttctaaatg ggaaagtgcc aaaaactatc cttccttaga tattttgatt gctatgagcg
      781 atttattcgg aatctcttta gagcatttaa ttaaaggaga tgcacgtttt aaaaaagtta
      841 ttttagaggg aaattataga gagagtaaca gggtaccttc catagttgat tttctttatg
      901 atttttggtg gcttttcttt cctgttgccg gatttttagt ttgggccatt cattcgttca
      961 tgggttgatt ttaaaatcaa aatttaatgc acatcaggca atatgaaaaa ggaaagtatt
     1021 tttacgacac cttgaaatgt attgataaga atagaagagt ttacaaaagg caatatgaaa
     1081 tatatactaa aagtaacaag aatttcgttt gtaaacgcac caaaccccga actattcgcc
     1141 gtagttcggg gttttttggt gaattggctt taatcgtcta tattattttt tatcacgttt
     1201 atggagctaa taaccaaata acgtaacgag acaaccactg attgccgttg tcatgatgtg
     1261 aacaagaatt tcattcataa atcgcacctc ctctctacgc cgaattgaca tctgagagat
     1321 agacgacctt cacattataa caaatctctc cttccatgaa ataacatgaa tattaataaa
     1381 tgttgttata tattaaagat aaataaatat aagggtggga ttgataatgt ttggcgatca
     1441 aagacaagaa gctacaaaat atgtcattaa agaaggttat caagatattt atttcctcaa
     1501 caaaaacggg gaatggtatt atttcgaagt aagaagtgtc tggcgtggca aacatattat
     1561 tagagttaaa gatggtttgt taggttggag aaaagaaata gtaactgaat aaagatacaa
     1621 aatacttgcc aatgcaaaag aatgaaaacg cgtcaaatca acgatgtata aaagatgcat
     1681 aaaagataat gaaagtaaaa agccataaat gccgtcatat aggcatttat ggctttttgt
     1741 ttggttaata taatcgatga ttatcagaag ttcaattcac ataaaaaagc acggctaatt
     1801 tatgaacttg tcgtaccttt ttaattaaaa ttagttacca aatagacgac tccaaaaccc
     1861 ctttttagct tccgtttctt cactcttttc ttctatatca ctatcattgt catttggatt
     1921 agctgaaacg tcttctcttt gtgcttcttc accttgtgta ctttcgtcgc tttgacttcc
     1981 agatatatcc ttatattgaa tctctgtttc tttagtttct tcctgatctc ggttaatatt
     2041 tacaggacta gtagtatatg ttttttcttg tgcatcaacg ttttgtctag cgttagtaga
     2101 tgtatcaaag ggataattaa ggtgtttttc tttttctaat tgattttcta atttttgaat
     2161 ctttttatta ctctctaatg ccagaacttg ctgattctca agcaattttg atctttcttg
     2221 caactctttt ctgtaaaatt caattgttgt ttcatgacga ctttcttgtt tttcaatttg
     2281 attatttaaa gtattaatct gatttttaag tatttctact atttgagcat cgcttttaga
     2341 ttcaaaatta atattatccg actctttttt ttgtttgtct tcagtagcac tttcaaaacc
     2401 atatttcttt ttattttcat ttactctttg aatgattttt tctatatcgg tatcttcttt
     2461 aataaacgaa gtgttttcct gttttattgt ctctatattc aatctcttaa tattattaaa
     2521 aatcgtttgc ttactaaccc ctaattcttc actcaacctt tttacacttt tcatatcatc
     2581 aaccctttat aaaatgttta taaaccctta gtaaaatatt ataagcttat aacgattaat
     2641 aataaagtaa tcgacaaata aaaatataaa ttcgccaacc tttttatcaa gggtttgtaa
     2701 agggtttatg gtgtttatca aaggtttata aacctatatt ttcccttttt taagtttatt
     2761 caaagtgcta taataacctt aatataacaa aaaccccaac tactggtaat agtcggggtt
     2821 tggtaataaa acgtttagaa actgtttatt tatataattt tcgatactta gattatataa
     2881 tgaataatat cttagtgcaa gcacctagat tattaaataa acacaaaaaa tgtaagcgtt
     2941 ttattacctc ttaccaaact aaaattcgga aagaggtttt tattatgtca attaaacttt
     3001 acaacataaa tgaggaatac agagaaaagt tttatcaaat accgaaagta ttttttacaa
     3061 atcctaaata tatcaagtta tccaatgatg caaaaatggc atggggtatt cttagagatc
     3121 gtttagattt atcaattaaa aatggttggg ttgattctaa aacaggtaat atttattttt
     3181 attacaaaaa tgaaaaccca caaaatatat taaattgtaa aaaagataaa gtaattaaaa
     3241 taaagaaaga acttaattga agccgaatta ctactacaag aaagacaagg tttaaataag
     3301 acaaataaac tttatttagt taaaccagaa gtaacagaag atgatattta taaaattcaa
     3361 aagcaagaaa acactgttga gagcagcaat ggcaaggaag tcggaaaaac cgacttcaag
     3421 aagtcgataa aacagaatct aatgatactg aacttagcta tactgaatct aacaatactg
     3481 aaataaatga tatgaatgac ttgaatgata tagagtccga aaatgaaatt agtaatcatt
     3541 caagtcaatg caaggttatt taatgcaaac aattttaact gaatttgaag aaacacatgc
     3601 actttatatg cgccgtaaaa acatgaaaca atataatatc tttaatcaat cattcaaatg
     3661 attttataac aatcaaagat acatcaaaat atttcgttaa atcatctgac tttttaactg
     3721 atagcgatga aaaaatcaag aaattgtcag tgatctagaa aaagatttgt atcgaaaacg
     3781 tatgttaagt tatggtggat tgcttaaata aaaacataaa attttaaact tagacgatgc
     3841 tgaagatggc aatttaatta atacaagtga tgaagataaa atagcaaatg aagaagaaaa
     3901 ggcacattcg attacggcaa ttttgaaaag caaaattatt atttaaggca ttagtgataa
     3961 ctgatgtctt tttttattac ttttttgtaa aaaaagtgct gtcttatttt tgtgacaaat
     4021 gctgtatgta gtgtcacaaa agtgtgacac ttgagctttt tatgacccca cacaaaaaca
     4081 ttttcgaagc cttgaaatat ctggctttgc cagatccccc attcattgat ggggacaaat
     4141 ttcccttatg ctcttacgga gattttagag ttaaattaaa atttctaagt ttttgataag
     4201 aaaagccctg caggaattta gaagaacgtg gggaagtttt aaagaatttt atgctaattt
     4261 ttaatttgca tgtaaatggg cagtgtctaa aaaatagtta ttgtttttgc tcaaattttt
     4321 gtttttgaaa atagcatata tttatttggc tcatatttgc gttttaaaag cgtatataag
     4381 ttttagataa aactatatga atcaccccta aatctttaaa atgccctctt aaaattcaaa
     4441 ataagggcat aaaaaataaa agccccccca gataaagtga ggtagctttt tctttcgacg
     4501 ccgtatcgtc gatgtaattc aaaaaagtta tgggctataa atctacatca ctttttcaag
     4561 aatgtagtag caatattcaa cgcaaattaa tttttataac tgctcggaat atctcaagcc
     4621 gtttctttaa ttttgaaata aaaaaatcga cgaaggtcga ttacgttttt gcacatgtcg
     4681 atttatccga cgtataagtg cgcccttacg ggatttaact agattataac gacgaatttt
     4741 agacctgtaa agcaataaca attaggtcta ccaggtagat aaagtgtaca aagtagaacc
     4801 gaaaaagtta tttattttga agatttttcg tagatataat ttgctaataa acttcctaaa
     4861 actgaataaa gaaaaatagt taaactcatg taatcactcc ataattctga agtgtctcca
     4921 tgtctacgca tagacacgca tttatgccga gaaaatttat tgtgcgttga gaagaaccct
     4981 taactaaact tgcagacgaa tgtcggcata gcgtgagcta ttaagccgac cattcgacaa
     5041 gttttgggat tgttaagggt tccgaggctc aacgtcaata aagcaattgg aataaagcac
     5101 ctatgattat ttaaaaatat aagttataat acattcaaaa atatttttga atgaggtgta
     5161 ttatgattca aacggttgtt gctgctgctg ttctttatat tgctacagca gttgatttat
     5221 tagtaatttt attaatattt tttgctagag caaagactag aaaagaatat agaaatattt
     5281 atgttggtca atatttaggg tctattattt taatattagt tagtttgttt ttagcttttg
     5341 tattaaatta tgttccagaa aagtggatat taggtttatt gggtttaata ccaatttatc
     5401 ttggaattaa agtggctatt tatgatgatt gtgaaggaga aaagagagct aaaaaagaat
     5461 tgaatgaaaa aggattgtct aaattagttg gtacggttgc aattgttacg atagcaagtt
     5521 gtggcgccga taatattggt ttatttgttc cttattttgt gacattaagt gttactaatt
     5581 tattacttac tctgtttgtc tttttaattt taattttctt cttggtgttt actgcacaaa
     5641 aattagctaa tatcccagga attggagaaa ttgttgagaa atttagtcgt tggattatgg
     5701 ctatcattta tatagcttta ggtttattta ttattattga aaatgacact attcaaacaa
     5761 ttttaggatt tatattttaa tttagggtgt gattttatat gagttatgaa aatacttgtg
     5821 atgtgatctg tgtacatgag gataaagtta acaatgcttt aagtttttta gaagatgata
     5881 aatctaagaa attacttaac attttagaaa aaatttgtga tgagaagaaa ttgaaaatca
     5941 tattatcttt gattaaagaa gatgagttgt gtgtttgtga tatttctttg atattgaaaa
     6001 tgagtgttgc ttcaacttca catcatttaa ggcttttata taaaaatgag gtacttgatt
     6061 tttataaaga tggaaagatg gcatattatt ttattaaaga cgatgaaata agagagtttt
     6121 tctctaaaaa tcaggagggt ttttgaaacg agtttcttct tgtcttgata ctataaaaaa
     6181 caaagttata taacataaga cgacttatcg gaagttatct atcatcgtat attcggagat
     6241 gatataatat acaggtcgcc tatctctcag gcgtcaattc agacgcagag aggaggtgca
     6301 ttactttgct aatcattttc gttcacatca tagcaccagt catcagtggc tgtgctgttg
     6361 cgtattatac ttattggctt agtaaacgca acaaataatt aataggcgac tttataagcc
     6421 agcatcgaaa aaatccccac actattgcag tagtgtgggg attggtgcat taattgttaa
     6481 tcattttcgt tatctagatt ataacactgg ttagtagagg aatgcaaaaa gataatattt
     6541 taagtagtct ttttggatat tatcatatga tatatttatg taaattgatt ttttattaaa
     6601 ggagcatata tgagtaatac taaaattaaa tatcaaattt ttatttcatc aacttattta
     6661 gatttaatag aagagcgtca agcagtagta gaagcagttt taaacagtaa taatattcca
     6721 gcaggtatgg aactttttaa ctcaagtagt aaaagccaat gggaaataat aacacaatgg
     6781 atcaatgaat cagatgcagt gatattattg ttaggttcta gttatggaac aattgatagt
     6841 gaagaaaata ttagctatac agaaatggag tacaattttg ctaaatcttt aggtaaacct
     6901 atatatgttt ttgagatgaa taaagcatct accgaatccg cggaaaagtc taaaaaatta
     6961 gatgagttta agacaaaagt taaagaaaat agactaatta gtccaaatat taatgatatt
     7021 tcggaattaa aaagtaaagt atctcagact ttaatgcaaa gtgtacctag tgaaattaaa
     7081 gaaaatagtg gatggataag agtacaggat tgtggtaaaa atgtaaaaaa attaaaaaaa
     7141 ttggatgcta tacaaagaga tttagataca tatgattttt tgaaagaaga actttataaa
     7201 atattcatgt atgtaaaaac tttttatttc cagcacacac catttgatga tagttatatt
     7261 gaagatatag aaaactacct gctaaattat gaaaataatc cacataaatc attcatgaaa
     7321 ataaaagttt atgattataa aaacttttta aaacatttaa aaaacatatt gattttaatt
     7381 aaacaaggaa aaaagccatt agctcaagcc ggtcatttta aattaagctt ttacgaagag
     7441 gatttaaaat tgttacaaaa aaattataag gctacgctag tcgatattaa aggattgaat
     7501 aaacattttt ataaggaatg tgataggtgc ttaaaagcat ataatatttt tattgagggt
     7561 ataaataaac atttatatga atgatataag tacaccctaa tcatacatat ataattgttg
     7621 tcactttact cgatatatga gaatgacttt acctaataaa tgagaaaagc aacgcactat
     7681 attaaaatgc gttgcttttt ctattatcga atacctataa ctaaactatc tctagctgtt
     7741 tctacaactt ctgtacttat tttatctaaa ccatttatat ccataattct gtctatctgc
     7801 gcaaataaac gatgaatcag cctaaaattc ccttttgtaa ttttaataat cgtcgttatt
     7861 gcctcataat cagtaaagtc ttcgagtttt aaatcaaatc ctaagtcttg ccacttatat
     7921 tctaatatat gatgcgtctc atctttactt agattatcaa attcatgcgc aaaacctata
     7981 cgcgaatata gttgaggata acgagatagt tttttctcta tacccggcat accaataaat
     8041 atcattgcta aattatgttc atcataaata gctcttaatt gctctaaatg ttgcacttta
     8101 aggcgatcaa tctcatctac tataattaaa tcaatatatt taaacatatc actataatgt
     8161 ttactatgtt catgtccata cttatttaca acatgtaatt tttgacccaa atcaatgatt
     8221 gcagtaatac tatataaatc attccttaat ttagtttgtt tttctgctgg tgcattataa
     8281 aatattttat ccaccgatag tatgtcatcg gtcgttttac tagctaaatc ttcccaacct
     8341 ctatggttaa cttgtttttc aatagtatcc caatttgtgt aatacctaga cgataaagtt
     8401 tttccgacac caggttgacc ataacatata ccaatatact gatatttaat acaagcatca
     8461 caaaattcag caaagcgttt atactctttc gtttcaatga aattttgatt tttagtcatt
     8521 acgatatctc tttaattttt tgacgttttt agtcgtttct tgaggtgaat aaccacaatt
     8581 tttttcttcc ttaattaatt ccgtggtact tggactagca atattttgtt ttaagtgttt
     8641 tcttctctga ctacgagcgt gctgtatgtc ttttatatct atggagtaat cagctatttc
     8701 tggacatata gcagtacaaa gaaattcatc tctataaaac acacgtattt cagccatatc
     8761 atttgggtta taacgaatca gtacatattc acctacataa gcttctagat tagtattact
     8821 gtatcgaaaa ccttgaaaat gaatgccatc tgaatgaact tttctagatt taggtatttc
     8881 caacaataat aaatcaagtt gttctaagct acttggcata ttagggaaaa agtgattact
     8941 attccaatga ctgataggcg tggtatgaat agtactatgt tctttttgat tataatcttc
     9001 aattaaaaag taacgtaatt tttcttcaaa attgtgaaaa tttattagat tactaggagt
     9061 atcattattg tttatatatc caggcagttg ttctaagaaa gtttgattaa ctgtctgaaa
     9121 gaatcgttct atttttccac gaccacgagg aacaccaact ttcgaaaaca tcaagttaat
     9181 tttcaaatca atagcgactt gttccatatg atgtgaagta aagtcacttc catgatctgt
     9241 gtagaatttt tcgggtatac cacacactgg ccaattggta tcatttttat tccatattgc
     9301 ttggtgtagt gttaatgctg tgttttgagc attggggggc atcaaaactt aagaaatagc
     9361 ctgcaattgc acgactatag tcatccataa taatagttag ccatggtcta ttgatattac
     9421 ctttttggtc taatataaag atatctagca aagtatggtc tgcttgccat atttcattgg
     9481 gtctcgagga ttctctaatt tgtattaaat cgtacttatt ttgatagtat ttttcaccct
     9541 gatgagagaa atcaataaca gacttaggca tcgctttaat aacactataa atttgtttat
     9601 aacttggtat atcaaaatta ttttctttac agtaattagt gattttccta tgaattgtgg
     9661 ctatactatt tcttttattc ataagcgcaa ggcgttcaat ttcatcaatt atctttggtt
     9721 ctattttacg tgttccttta tcagctcttg tcgcatagat tagtccttta agtccttgtt
     9781 ctttatatga cttattccac ctataaaggg tgcttaacgc aatcccttta cttttagaaa
     9841 tacttgataa agattgttcg cctaggataa aaggttttat aatattgtac ttttgagtag
     9901 cttttttacg tttggcttca gaaaaattag ttagatactt ctccttgttt ttcatcttcc
     9961 ttgtcctcca atttattgag gtaacgataa attgttgtac gtgaaacatg ccactgttct
    10021 gcaattgtct taattggcgt gccattatca taaagggtct taagtaagtt taaatctttt
    10081 tgatttaatt tttctggtct gcctccatat cggcctctag cacgtgctgc aattcggcca
    10141 gctgaagaac gctctaaaat taaattacgt tcaaattctg caaatgctgc gaataaatga
    10201 aataataatt gtccagttga acttgattta tccattgtta tattttcttc taaactatgg
    10261 aaacttacac ctcgttcgtt aagctcatta actaatgtga ttaaatcctc catattacgt
    10321 cccaatctat ctaatctcca aacaacaatc gtatctcctg acctcgcaaa ttcaattgct
    10381 ttatctaaac caggtctttt actttttgaa ccactcatat ggtcattaaa tatcttttca
    10441 caaccatatg tatttaatcg atcctcctgt aaatttaaat tttgtaatcc agttgaaact
    10501 cttgcataac ctattttcaa aatacaaacc tcctatatac atcttcagtg taccataact
    10561 taattacaag gtcttttatg aacatagatt ttttgttggg tttttgaaca cataatccta
    10621 tgtaattctt acttatttag acataaaaga agagtgtaca gaaaatggtt attttctgta
    10681 cactctcatc tttaataata tattttaaat gtcattaaca aaactaattt aataagagtc
    10741 aagcatagtt tacatatgta ttatgtttga aacatacctc taattacttt ttactaatat
    10801 catttaaaat atctcgcaat tcttcaattt ctttgttatt taattcttca tttttcgcaa
    10861 aattcaacac taaacttttc atatcccctc catacagttt attaagaaag gttttagcag
    10921 ttttcatttt aatatcgtct tctttaatat ttgatgagta aaaataaata ttttctgatt
    10981 tgtatcgttt tataatctct tttttatata gtcttgtgat taatgttcta atcgttttat
    11041 cactaacttc tttatatttt tgaatttcaa ctacaatttc attagctgat actgattttt
    11101 tatcccatat tatattcata acatcccatt cagccataga tatttcaact tgcttattgg
    11161 ccatttaaaa cacccattcc ttttaatatt ttttcactta ttaattcagc atttttccca
    11221 gacgggtttc catctgataa atgtgtagca aaataatact tatcatgatt tgtaattacg
    11281 taacctacaa accacccatt attatacttc ccgtttacta tacctgtacc tgttttccca
    11341 tacagttcat acttttcatt tttcttaatc aataatgaag aagataattg attctttgct
    11401 tttttactaa aatggttatt ttgttccatc atatttttaa aaactattac ttgttcaaga
    11461 ttagatattt tcaaactatc ttccatccaa tagcttttat aacttcccaa attttcatta
    11521 ccataattta attgcttgag ttgagccgca gtatagttct ttggtatttg attactaata
    11581 cgttcgaagt accaattaac tgaattttgc attgctgtat ttaaatcttg ttccttattc
    11641 caagactcga aaagataatg tttatgattc catgacatac gtgaattttt atcacttata
    11701 atatgacggt caagtccaaa cagtgctaaa taaattttgt aagttgaatc aggagaatat
    11761 ctttttctac tttctttttc attataaata tagtatttgt ctttcttcat gctgtacata
    11821 acgaaagatc cactatttga accaaaattt ttactttcat ctaaaatttg gtaatcgctt
    11881 tgtaagggct ttttataatt ataatcagtt aaggattgcc ccattaaaaa ctggctttga
    11941 attatcatga taaaaaaagt aaaaatacaa ataaaaatta gaattaattt tgactgcttt
    12001 ttcagatcgg cttcttttat attaattaat ctatttttga gtaatgattt tttaccatta
    12061 aatgaatggc ttaatatatt tttgttgaaa aatgaggttt ttaatacgga gtccataata
    12121 gattccgcat aagttttaaa ttcatttttg ttaatattat taaggacaaa tctatcggct
    12181 tctacttcat tgtcatgaat tatctttctt tttacaatat gtactaatgg attgtaactc
    12241 ataataatag agaagatatt aaagataatt aaatgtaaag tatctctatt tttagcatga
    12301 gcatattcat gcagtattat atattttagt cttttatcaa ttacactttt aaaatatgaa
    12361 ctaggaatca aaataatata tttcccatac caaaaagtta ttggagattg aatagcctct
    12421 gcttttcgaa tcacaatatt ttttttatat tgatggttga aaagtatcgt atctatttta
    12481 tttttttcat tttcgtttag ataaagtgac tgtttcttta aatatttaag atataataag
    12541 gctttcaaaa atttaaaact taaaataata actaaaacta tccaaataac tgtgcagata
    12601 ttatcaattg aatcccaatt aaacttatgg atatctgttg cgaactcttg aacaggtttg
    12661 gtggtaatta tgttatggtt caagtcgtgt gacttacttt caactgtggg cgcttgatta
    12721 ttcacattat taaattttat aaaagagaat ttaattggga taaaaggaat taatccagca
    12781 agcacagtta gataccaaac tttataattt aaactataat taaaatagcg ttttaatatg
    12841 tacctaaaaa atactaacaa tagaaatata aaacaaaagc ttactacact cattattagt
    12901 aatttagcca ttttgacacc ttctttcaaa tatttataat aaacaattga catcaatatt
    12961 acaattgtaa tattattgtt ttgtaaaaat tacaactgta atatcggagg gtttattttg
    13021 aaaaagttaa tatttttaat tgtaatggct ttagttttaa gtgcatgtaa ttcaaacagt
    13081 tcacatgcca aagagttaaa tgatttagaa aaaaaatata atgctcatat tggtgtttat
    13141 gctttagata ctaaaagtgg taaggaagta aaatttaatt cagataagag atttgcctat
    13201 gcttcgactt caaaagcgat aaatagtgct attttgttag aacaagtacc ttataataag
    13261 ttaaataaaa aagtacatat taacaaagat gatatagttg cttattctcc tattttagaa
    13321 aaatatgtag gaaaagacat cactttaaaa gaacttattg aggcttcaat gacatatagt
    13381 gataatacag caaacaataa aattataaaa gaaatcgggg gaatcaaaaa agttaaacaa
    13441 cgtctaaaag aactaggaga taaagtaaca aatccagtta gatatgagat agaattaaat
    13501 tactattcgc caaagagcaa aaaagatact tcaacgcctg ctgctttcgg caagacttta
    13561 aataaactta tcgcaaatgg aaaattaagc aaaaaaaata aaaatttctt acttgattta
    13621 atgtttaata ataaaaacgg agacacttta attaaagatg gtgttccaaa agactataag
    13681 gttgctgata aaagtggtca agcaataaca tatgcttcta gaaatgatgt tgcttttgtt
    13741 tatcctaagg gccaatctga acctattgtt ttagtcattt ttacgaataa agacaataaa
    13801 agtgataagc caaatgataa gttgataagt gaaaccgcca agagtgtaat gaaggaattt
    13861 taatattcta aatgcataat aaatactgat aacatcttat agtttgtatt atattttcta
    13921 ttatcgttga catgtataat tttgatatca aaacctgatt ttccctttat tattttcgag
    13981 atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa aaatcataaa
    14041 taatagatga atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt
    14101 aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat caagcaaagt
    14161 gacataattg ttgaatttat agtaactgaa acgtatgatt agggtgtata ttaattatga
    14221 gatggaaatc aatgattgtt ggctatgcga gagtatcatc gaacgatcaa aatttagaaa
    14281 gacaacttga aaatttaaag acgttttgtg ctgaaaaaat atttacagaa aaacagtctg
    14341 ggaaatctat tgaaaataga ccagtacttc aagaagcact taactttttg aggatgggag
    14401 atcggtttgt agtagaatcc attgattgtt taggtcgtaa ttatgatgaa gtggttggca
    14461 atccattact ggataagttt atgaaagatt taattattca aatattagca atggtttcag
    14521 aacaagagag aaatgaaagt aaacgtcgac aagcgcaagg aattcaagtt gcgaaggaaa
    14581 aaggcgtata taaagggcgc cccttacttt attcaccaaa tgctaaagat ccacagaaac
    14641 gtattattta tcatcgcgtt gtagaaatgt tagaagaagg ccaatcaatt agtaaaattg
    14701 cgaaagaagt aaatataaca aggcagacga tttacagaat taaaaacgat aatgatttga
    14761 tttaataaac tttgtttcaa tgcaactgag agcttctcac agcgtctcaa aacagatgat
    14821 tataatctat tatgagtttt taataataag acaagaaaag agagcgtaaa tatgctctct
    14881 ctttttctca ttaacgaacg tttataacgc aatcatgatg agagtattaa tacagttaat
    14941 tatttgaaat actttagttt atataatgtt gaattatagg tagtgaaatt gtatttatta
    15001 tacattgtca tatcgttgtt taatgatagt taatttaagg ataatagaat taatattata
    15061 tatagatgaa caaaacaagc aacagagcag aaaaaacact tttagattat ttttactcat
    15121 tctctatttt atttattttt gctataataa ataaagaata ttaaatggat gtgtttttat
    15181 gaaaaagaaa tttgtttcta gttgtattgc tagtacaatc ttattcggca ctttattagg
    15241 tgtaacttat aaagctgaag ctgcaacagt acatgtagct ggtggtgttt ggagccatgg
    15301 tattggaaaa cattacgtat ggtcttatta tagtcacaat aaaagaaatc atggttcaac
    15361 agcagtaggg aaatattcat cttttagtgg tgttgctcga cctggtgttc aatctaaggc
    15421 atctgctcca aaggcttggg gtggaaacaa aacattttat agtcttcact aattacatat
    15481 attaattaag gagaatagac agaattaagt aaacttaatt ctgtctattc tcttgaaaga
    15541 ggcaatatga aaaaattcaa attattttta gattttagta ctttattatt aatttcaggg
    15601 ctattatttt tatttttctt caaagaaaat gaagaaatta tacctgaaag ttccaatatt
    15661 ttaacaatct ctaattggga taaatctaac agtaaatcaa aagtgttaga tgtcattgaa
    15721 tcaggcgcca aaaatcaaaa tatacaaata attaaatccg ttaaagattt tgataataaa
    15781 aaagaatttt ttgttttcaa ctcaaaacga aataattctg actttatacg taataaaaca
    15841 tcacttttaa ctccatcaga tttattaaac agagagataa aaggaaagta ttatattatt
    15901 ggtgaacatt tcaatgtaga agagcttacg agtgaattaa aaaaagctgg tttaacatct
    15961 gaagttgaat acataaatag aaacatatta ttttttgaat tagtcataga taatgattta
    16021 ttagtgcctt tcatattctt aggcgtatta tatcttttgt acttcttata tgacagagga
    16081 tataatttga aattttatgc tattcaaaag ctacatggct tttcaactac acgcattatt
    16141 tttcataata ttcatataaa aatcatttat tggctagtac ttaccgctat tttctttatt
    16201 gctaatatat taatcattca tatagccaat ttagaattgg atttcttaat gtttattcgt
    16261 agatttatcg tgttgctttt tgtttttgct tgtattacta cattgctgtg gctgatttcg
    16321 tatagtcttc ttttgaagtt aagtattcca ttaatcttaa aagggaaaaa aggatataag
    16381 ttttcaatat ttatgggtac cttcaccaaa tgtatcatgg tgctaatact atctttttta
    16441 ctggcacaaa atttaaatgc ttatacgaag ttaaagaata tatatgactc tgaaaaaatc
    16501 tggcaaaagt tagataatta ctatacatta gagatttctc ctaatattag aaattcagaa
    16561 gaaaaacaaa ttttagaaac taatatatac aatttaatta agaaaagcga acaacttggt
    16621 ggattgcttt taaagaataa taatatagcc aatcccgata aaaacaatta cattcctgaa
    16681 aatggcaatg tcttttttat caataacaat tttctgaatt tctataaaaa tataaatcat
    16741 gattttaaaa tgattgacaa tcattcggat aaaataaacg tgtttattcc tccaaggtta
    16801 caatatcaaa aatctgaaat tcaaaaaaat caccaaggat ggattgactt tcaaaagcaa
    16861 caaaacaaaa aagtagatgt gcaagtactg tcaaaaaacg tatctgtttt ttcttttgat
    16921 cgcacaacta atttgaaatt tgggtatcta gattctccaa tagtaatgtt gttgacactt
    16981 gacgatttaa caggagattt ttatttagct gctgtttctc aaggtggata tctatttaaa
    17041 gatttagata cacttaaaca gttgcttaaa gataatcacc tagaagaaga aattagcggt
    17101 ataactaatt acaaggattc tgtgttaaat gaattaaatg aaacgaaaac aaaaatgata
    17161 ataacaatag ttactattat tattaacgtt ttcattcttt tgatagctac tgtatttgaa
    17221 acaattcaat attttagctg gaataaaaag caactattat taagaaaaat tcatggttat
    17281 tcattattca gcagtaatgt acgttattta acaatttcta tcttactttc tattatgtta
    17341 gcttacacta cgcatatttt atttggttca aagatattac tattcataat aatgtctatt
    17401 gctattattc aacatttact tcaaattgct tatattaaat atcttgaaaa atactttaaa
    17461 gatttaatga gggagattta aacaatgaaa agaactttaa ttattgtaac aattgtacta
    17521 ttaatcatca tacttagtct gttattggtt agaaatgagc atttagatcg ttttaaccca
    17581 ctgttaaaag aaaaagtatc ttatgccaaa gttccaaaag atacccagga ctatagaaat
    17641 ataactgtat actctaaaaa tggagaaaaa aagagttata aattggattt tttaggttat
    17701 gatcctacaa aagaatatgt gaaagtaaat cataaaggga aatatgtgcg ttcgatagaa
    17761 tacattacaa aagatattcc tagcttttta aaatgaggtg accgatagtg ataaaacttg
    17821 aaaatgtttt tgtaaaaaaa ggtaataaaa acattttaga cggatgtaac tttaattttg
    17881 aaaaaggaaa atcatatgct ttagttggag aaagtggcgc tggaaaatct actttactta
    17941 acataatcgc tgggttcgaa gatgtatcac aaggaagcat atatattgaa gataagttac
    18001 taaaaaagaa agtcgatttt tatagatata cgttagggta tttgtttcaa aattttgctt
    18061 tacttgaaaa tcaaacaatc agtcaaaatt tagatttagc cctaaagttc aaaaagaata
    18121 aaaaagataa tatgaactta aagaaagaag tattaaaaaa ggtcggccta gatttagata
    18181 tcaagagaat tgttagttct ctaagcggtg gtgaacaaca aagagtagca ttggcaagat
    18241 taatattaaa agatccgaaa atcattttag ctgatgaacc tactgggtca ctagatacga
    18301 aaaatggaaa aatagttatt gatttattat tgaagctact tgatgaaaat aaaactatga
    18361 ttgtcgtaac gcatgattta gagcttgcga aacgatttga tgtcatagtc aatatttctg
    18421 agcttagaag ttaagaaata ttgataggta tagccataca tagagaaaaa acaatatgaa
    18481 atatgattag tcattacttt tgagtttttt aatttaagga ctggattttc cagtcctttt
    18541 ttatgatgac aaaaaacaag cctcgcagag cacacgtttt taatgactta tttggttttt
    18601 aataagtcta gtatgttaaa acttttctgt gtttaggtgt tataatcata tgcagtggaa
    18661 gttgatttat tatgtcttat tcaattattc gagttgttaa agtgaaatca aaaacaaata
    18721 caagaggtat tcaaagacat attcaaagag aaaataaaaa ttatgaaaat atagatattg
    18781 atttaagtaa gtcatattta aactatgatt tagtcaatga tactaagttt gattttaata
    18841 aaaaaattga tgagaaaatc gaaaaaaatt ataaagggaa acgcaaaatt agaacagatg
    18901 ccattaaaca tattgatgga ttaattacat cagataatgt gttttttaat caattatctg
    18961 aagaagagac aaaatagttt tttaagcatg ctaaagcgtt cttggaagaa gaatacggta
    19021 aagaaaatct tttatatgcg acagtacata tggatgagat aacaccacat atgcattatg
    19081 gcgtagttcc tattactaaa gatggacgtt taagtgcgaa agaagttgta ggtaataaaa
    19141 aagcattaac cgaattccaa gatagattca atacgtatat taataagcaa ggctatgatt
    19201 taaaacgagg aatttctaga caacttacaa aagaaaaaca tgatcaagtt agccgttata
    19261 aacaaaaaac agaatatcat aaacaaatgc atatgcgcta gaagcaaata gaggattatt
    19321 taaagcaaga aaatgataaa ttgatacaaa actaccaaaa atcattagat acgcttaaaa
    19381 agcttctgag tgtcatgtat gaaattaaaa ctaaaaatgt aggtggatgg tttcataaag
    19441 agaagcaaga aacaggcaat attgtaatta ctaaaactga ttttgaaaaa tacactaaac
    19501 aaattaaagc agcacagatg atattagatg attatgaatg tattaaaagt gggaagtcat
    19561 taaaaaaagc agagaaacaa aatgaaagtt tagttaatga attgactagt gtccatatgg
    19621 aaaatgaaaa gctggttgaa gaatttaatg atttagcaca acgttataat tatttgttgt
    19681 cagataatga aaagaaagat aaagaactaa attatacgct taagttattc aatcaagttt
    19741 ttaaaatcat taaaagtatg atgaaagaag agcgttatca tacactaata aatcatattg
    19801 ataatcattt agacaattct aaaatacgtg aagtaatgac gatagataac aatgatgaac
    19861 agttctttaa gaagaaatat caagcacaag aacgagaaat catttttaaa gaagatagag
    19921 aagatggtta tacattataa aaatagatat ttaaatgcat ttgtttgagg ttaattattt
    19981 tttaggatga aaaataggta gcccccctcg aactttttaa tatattttta taaatctttt
    20041 taaaaacctt tttaggggct tgttcagccc tagagcaaca agggatttca atgcctaaag
    20101 gagtgttttt gccacataaa aggagtgttt ttgccacata aaaggagtgt ttttgccaca
    20161 taaaaggagt gtttttgcca cataaaagga gtgttttaaa aagttgtact ccttttatgg
    20221 tttgacattg ataataaagt agtttattgt gaataaaagt tttcaaatgg gagtgtatat
    20281 tcaaacgata ctcctttagg ttcgtaataa agggggctgt atcatgacag gagagacagt
    20341 tgtatataaa aatgaaatga atttagttcc attaaggcga tttacagcta ctgaaattaa
    20401 tttatttttt gcgatgtgta ataaattaaa agagcaagat actaatacat tacgtttgtc
    20461 ttttgatgaa ttaaaaaaat taagtaatta tagtccagaa acacgtaata ttaatagatt
    20521 tgctaatgat ttagataatg tgtataaaaa gatgttgaat ttaaccatta gatatgaaga
    20581 tgatgatgta atagaaagat tcgttttatt caatcattat agaatccata agcgagaaca
    20641 atat
//
