LOCUS       NZ_CP092053            13162 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Staphylococcus aureus strain USA300 plasmid pAH5667_1, complete
            sequence.
ACCESSION   NZ_CP092053
VERSION     NZ_CP092053.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25144899
            Assembly: GCF_022226995.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13162)
  AUTHORS   Horswill,A.R.
  TITLE     Genome Sequences of Two Methicillin-Sensitive Staphylococcus aureus
            Healthy Skin Isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13162)
  AUTHORS   Horswill,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) Center for Genes, Environment & Health,
            Department of Immunology & Genomic Medicine, National Jewish Health,
            1400 Jackson St, Denver, CO 80206, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022226995.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:25:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,847
            CDSs (total)                      :: 2,765
            Genes (coding)                    :: 2,696
            CDSs (with protein)               :: 2,696
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 38 of 69
            Pseudo Genes (incomplete)         :: 33 of 69
            Pseudo Genes (internal stop)      :: 13 of 69
            Pseudo Genes (multiple problems)  :: 14 of 69
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092053.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13162
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="USA300"
                     /isolation_source="skin"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pAH5667_1"
                     /geo_loc_name="USA: Colorado"
                     /lat_lon="39.74 N 104.99 W"
                     /collection_date="2021"
                     /collected_by="Alexander R. Horswill"
     gene            join(13142..13162,1..963)
                     /locus_tag="AH5667_RS14135"
                     /old_locus_tag="AH5667_002678"
     CDS             join(13142..13162,1..963)
                     /locus_tag="AH5667_RS14135"
                     /old_locus_tag="AH5667_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001181102.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRFSNVQKFENRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            <983..1273
                     /locus_tag="AH5667_RS14140"
                     /pseudo=""
     CDS             <983..1273
                     /locus_tag="AH5667_RS14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            1817..1966
                     /locus_tag="AH5667_RS14145"
                     /old_locus_tag="AH5667_002680"
     CDS             1817..1966
                     /locus_tag="AH5667_RS14145"
                     /old_locus_tag="AH5667_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000695366.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(2134..2826)
                     /locus_tag="AH5667_RS14150"
                     /old_locus_tag="AH5667_002681"
     CDS             complement(2134..2826)
                     /locus_tag="AH5667_RS14150"
                     /old_locus_tag="AH5667_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity/bacteriocin fusion bifunctional
                     protein"
                     /protein_id="WP_000691527.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="putative immunity/bacteriocin fusion bifunctional
                     protein"
     gene            2936..3130
                     /locus_tag="AH5667_RS14155"
                     /old_locus_tag="AH5667_002682"
     CDS             2936..3130
                     /locus_tag="AH5667_RS14155"
                     /old_locus_tag="AH5667_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001004925.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            4247..4864
                     /gene="cadD"
                     /locus_tag="AH5667_RS14160"
                     /old_locus_tag="AH5667_002683"
     ARG             4248..4861
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4883..5230
                     /locus_tag="AH5667_RS14165"
                     /old_locus_tag="AH5667_002684"
     CDS             4883..5230
                     /locus_tag="AH5667_RS14165"
                     /old_locus_tag="AH5667_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            5387..5479
                     /locus_tag="AH5667_RS14170"
     CDS             5387..5479
                     /locus_tag="AH5667_RS14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5651..6622)
                     /locus_tag="AH5667_RS14175"
                     /old_locus_tag="AH5667_002685"
     CDS             complement(5651..6622)
                     /locus_tag="AH5667_RS14175"
                     /old_locus_tag="AH5667_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_000481279.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            6746..7165
                     /locus_tag="AH5667_RS14180"
                     /old_locus_tag="AH5667_002686"
     CDS             6746..7165
                     /locus_tag="AH5667_RS14180"
                     /old_locus_tag="AH5667_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000418035.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            complement(7691..8536)
                     /gene="blaZ"
                     /locus_tag="AH5667_RS14185"
                     /old_locus_tag="AH5667_002687"
     ARG             complement(7695..8536)
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8643..10400
                     /gene="blaR1"
                     /locus_tag="AH5667_RS14190"
                     /old_locus_tag="AH5667_002688"
     ARG             8644..10397
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            10390..10770
                     /gene="blaI"
                     /locus_tag="AH5667_RS14195"
                     /old_locus_tag="AH5667_002689"
     ARG             10391..10767
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            11034..11612
                     /locus_tag="AH5667_RS14200"
                     /old_locus_tag="AH5667_002690"
     CDS             11034..11612
                     /locus_tag="AH5667_RS14200"
                     /old_locus_tag="AH5667_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690637.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(11973..12716)
                     /locus_tag="AH5667_RS14205"
                     /old_locus_tag="AH5667_002691"
     CDS             complement(11973..12716)
                     /locus_tag="AH5667_RS14205"
                     /old_locus_tag="AH5667_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843732.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     ARG             complement(7692..8536)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          1329..1476
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.644"
                     /coverage="100.0"
                     /evalue="1.3e-63"
     Typing          2..963
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.896"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          1095..1265
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="91.0"
                     /evalue="6.2e-68"
     Typing          complement(11974..12716)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcaagaaa attacaaaga aagattttat caaatcccta aagtattctt taccagtgaa
       61 aattataaaa atctaactaa tgatatgaaa atagcctatg ctatcttacg tgatcgctta
      121 aatttatcta ttaaaaatag ctgggttgat gaagatggta acatttactt tgtttattca
      181 aatgaaaagc taatggaaat cttaaattgt aaaaaagaaa agttaacaaa aataaaaaaa
      241 gggttagaaa acgatggtct actcattcaa aaacgacgtg gtttaaataa accaaatatt
      301 ctatatttaa tgaaacctat tgtcacagaa cgtgatattt acaaaataga aaaagaagaa
      361 aacgatgttg aaccttacgg cgagaaggaa gttcgatttt cgaacgtcca gaagttcgaa
      421 aatcgaacgt ccagaagttc gaaaatcgaa cgtccagaag ttcgaaaatc gaacactaat
      481 gatactgact ttaatgacat tgactttaat gatactgaga ataatgatat gaatgacttg
      541 aatgatataa agtacaaaaa tgaaattagt aatcattcaa atcattcaaa tcaatttaca
      601 cataattttg atgataaaga aatgctattg caagaattcc cagaacaact aactaattat
      661 ttattaaaat atgattatag agatttagaa atcataaagg ctgttattct taaagcaaaa
      721 aaatcattta attcaaggca tgaagatatg cattacatgt tagaggacat tgaggatgaa
      781 atattaactt ctttaaaaag acttaaaaaa gctatacatg ataggggagt taaaggccaa
      841 aaggaaacaa tcaagtcaat gcaagcttat ttaatgcaaa caattctaac tgaacttgaa
      901 gaaacacatg ccctttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa
      961 taattcaaat gattttataa cactcgaaga gacatcaaaa tattccgtta aatcatctga
     1021 ctttttaact gatgacgacg aaaaaaatca agaaattgta agtgatttag aaaaaggttt
     1081 gtatcgaaaa cgtatgttaa gttatggtgg attgcttaaa caaaaacata aaattttaaa
     1141 cttagacgat gtcgaagatg gtaatttgat taatgcaagt gatgaagata aaacaacaga
     1201 cgaagaagaa aaagcacatt caattaccgc aatttggaat ttcgaaaagc aaaattatta
     1261 tttaagacat tagtgttgac taatgtcttt tttgttgatt ttttataaaa aagtactgtc
     1321 ttatttttgt gacaaatgct gtatgtagtg tcacaaaagt gtgacactac agctttgtat
     1381 gatatcactt taaaatacct taaaacccct ggaatttctg gctttgccag acctatcatt
     1441 tttgaatgat agcaaattct ccttatgctc ttacggagtt tttagagaaa aattaaaaat
     1501 tctcgatttt tgataaaaaa cgccctgcag gaatttagaa aaacatgtgg aagttttaaa
     1561 ggattttatg ctaattttta atttgcatgt aaatgggcag tgttaaaaaa ttagtcactg
     1621 gttttgctga aatttttgtt tgtgaaatta ccatatattt atttggctca tatttgcgtt
     1681 ttaagagact gtatagattt ttaagtacaa aacctatatg atttgcctct aagtcttcaa
     1741 aatgcctctt aaaatccaaa ataaaagtgt ttaatattgt aataaggatt gctattttta
     1801 taaaaggaga gatatcatga agattaaaac tttcgctact tttactcatc gtcaattaga
     1861 taaacaagtt aataggtttt taaagaatga tattcatgta ttagaaattc aaacacacag
     1921 taattggtta tatatttcag ctattgtttt ttatgaggat aaataaggaa catcgtattt
     1981 atgccgaaaa aatttattga tgttgagaag aacccttaac taaacttgca gacgaatgtc
     2041 ggcatagcgt gagctattaa gccgaccatt cgacaagttt tgggattgtt aagggttccg
     2101 aggctcaacg tcaataaaac aattggaata aaatcaacct acagagcatc cagctgcaaa
     2161 tgcagttccg caaactgctt cacatacacc gccggctatt ggatttacga ttgcccatac
     2221 tccacagtag tgagcacatg cataaagtcc agccatacta caagcgaatg ctttgccgtt
     2281 ccatttgaat cctgcatatg ttaccggtct gtttttatca actttttcag ccttatattc
     2341 aacgtaattt ttgttaataa gtatttttcc ctctttgttt tcagtcatac ttcttttttc
     2401 ggcaatgaat tttgtgattt ttttgttgta gttatcatat attgtttcag aaactacaat
     2461 ttcttttgtt ttttcatttt tataagcgtt attagctatg tagccatatg catttttatc
     2521 atttgcttct gtagctagat attttaattt agaatttttg tcttctagct ttattttttt
     2581 aaatccttct tttttcattt gcttttcgaa tttttgcata tcttcatttt tattttgcaa
     2641 ttgttctaga aaacttattt ctttcttggt catttgtgat acttcgatgt ttttgctttt
     2701 caatttttct ttttgatttt ctagtaaaga acttttttgt tcttcatttg cgtaagcatt
     2761 agaaaaattc gcgctaaata ccagtaggaa caaggaaatt attgaaaata attttataat
     2821 tttcatttga tacctccaag ataataagat attttaattg tataagtaag cgctttataa
     2881 aatcaatatt tattttaata tatttataaa catatttttg aaaggagtct attttatgaa
     2941 tgaaaattta gaaaaatata taaaaagttt gccactttta ggtttgttaa ttagtatttt
     3001 gttaattgtt ttattctttt ttattttaga tgttgatgaa gatttattcg tggttttttt
     3061 atactgttta ttgcctttga tagttaattt gtctatttat ggtttttatg tttttgttaa
     3121 aagaaagtaa gattatgaat aattttgttt ttaatttgta tgctttgagg gggttggaaa
     3181 acggtaggtg tatagattag atttcttgtt tttttgaaac gagtgtgaac gagtttcttc
     3241 ttgtcttgat ttttgataaa aacgccctac aggaatttag aaaaacgtgg ggaagtttta
     3301 aagaatttta tgctaatttt taatttgcat gtaaatgggc agtgtctaaa aatagttatt
     3361 gtttttgctc aaatttttgt ttttgaaaat agcatatatt tatttggctc atatttgcgt
     3421 tttaaaagca tatataagtt ttagatataa aactatatga atcaccccta aatctttaaa
     3481 atgccctctt aaaattcaaa ataagggcat aaaaaataaa agctacccca gataaagtga
     3541 ggtagctttt tctttcatac ggtctattta tttttctttc gacgccgtat cgtcgatgta
     3601 attcaaaaaa gttatgggct ataaatctac atcacttttt caagaatgta gtagcaatat
     3661 tcaacgcaaa ttaattttta taactgctcg gaatatctca agccgtttct ttaattttga
     3721 aataaaaaaa tcgacgaagg tcgattacgt ttttgcacat gtcgatttat ccgacgtata
     3781 agtgcgccct tacgggattt aactagatta taacgacgaa ttttagacct gtaaagcaat
     3841 aacaattagg tctaccaggt agataaagtg tacaaagtag aaccgaaaaa gttatttatt
     3901 ttgaagattt ttcgtagata taatttgcta ataaacttcc taaaactgaa taaagaaaaa
     3961 tagttaaact catgtaatca ctccataatt ctgaagtgtc tccatgtcta cgcatagacg
     4021 acgcatttat gccgaaaaaa tttattgatg ttgagaagaa cccttaacta aacttgcaga
     4081 cgaatgtcgg catagcgtga gctattaagc cgaccattcg acaagttttg ggattgttaa
     4141 gggttccgag gctcaacgtc aataaagcaa ttggaataaa gcacctatga ttatttaaaa
     4201 atataagtta taatacattc aaaaatattt ttgaatgagg tgtattatga ttcaaacggt
     4261 tgttgctgct gctgttcttt atattgctac agcagttgat ttattagtaa ttttattaat
     4321 attttttgct agagcaaaga ctagaaaaga atatagagat atttatgttg gtcaatattt
     4381 agggtctatt attttaatat tagttagttt gtttttagct tttgtattaa attatgttcc
     4441 agaaaagtgg atattaggtt tattgggttt aataccaatt tatcttggaa ttaaagtggc
     4501 tatttatgat gattgtgaag gagaaaagag agctaaaaaa gaattgaatg aaaaaggatt
     4561 gtctaaatta gttggtacgg ttgcaattgt tacgatagca agttgtggcg ccgataatat
     4621 tggtttattt gttccgtatt ttgtgacatt aagtgttact aatttattac ttactctgtt
     4681 tgtcttttta attttaattt tcttcttggt gtttactgca caaaaattag ctaatattcc
     4741 aggaattgga gaaattgttg agaaatttag tcgttggatt atggctatta tttatatagc
     4801 tttaggttta tttattatta ttgaaaatga cactattcaa acaattttag gatttatatt
     4861 ttaatttagg gtgtgatttt atatgagtta tgaaaatact tgtgatgtga tctgtgtaca
     4921 tgaggataaa gttaacaatg ctttaagttt tttagaagat gataaatcta agaaattact
     4981 taacatttta gaaaaaattt gtgatgagaa gaaattgaaa atcatattat ctttgattaa
     5041 agaagatgag ttgtgtgttt gtgatatttc tttgatattg aaaatgagtg ttgcttcaac
     5101 ttcacatcat ttaaggcttt tatataaaaa tgaggtactt gatttttata aagatggaaa
     5161 gatggcatat tattttatta aagacgatga aataagagag tttttctcta aaaatcatga
     5221 gggtttttga aacgagtttc ttcttgtctt gatactataa aaaacaaagt tatataacat
     5281 aagacgactt atcggaagta gatatgcttg tatattcaca gatggtataa taatagagtc
     5341 gcctatctct caggcgtcaa ttgacgaaga gaggaggtgc attactttgc taatcatttt
     5401 cgttcacatc atagcaccag tcatcagtgg ctgtgctgtt gcgtattata cttattggct
     5461 tagtaaacgc aacaaataat taataggcga ctttataagc cagcatcgaa aaaatcccca
     5521 cactactgca atagtgtggg gattggtgca ttaattgtta atcattttcg ttatataaat
     5581 tataacactg gttagtagag aaatgcaaaa tgattgaatt aatagtaaat gtattaatga
     5641 atactcacac ttatactttt aaaatagttc ttcctaaatg ttgtcctttt aaaagctgat
     5701 ctatcgtttt atagaattct tcaaatccaa tttcattgta tagaagtgaa tttcctatat
     5761 tccattcatt tgctaactta ctccatatat cttcacggat attatgatca acatatacag
     5821 aatcaatgcc aattaattta atacctctaa gaataaacgg taatatatta gcgttaagtg
     5881 aatttcctgc tacatttcca catacagtag caacaccttg ataatttaga cgttttaaac
     5941 cgtataatgt tacattactc cccactgtat ctaagaaata atcaaaaata ccttttttaa
     6001 gcggtttatt atcatcttca attattatta cttgttttgc acctaatttt tttgcgattt
     6061 ctatttgctt ttcttttctt acgatagcag tgatattttc atatccaatt ttatgtaaaa
     6121 tttgtatagc aacactgccc acaccacctg tagccccaga cactaaaatt tcaggttgtg
     6181 atttgatatt cattccatat ttttctagtt caaaaattga taatgctgcc gtaaaaccgg
     6241 ctgtgccata taccatagac tctttaatag acatttcttg gggaatagga acaatccact
     6301 ctccaggcac ttgtgcatat tctgaaaatc cacctgtatg tgtcacgcca acatcataac
     6361 cagtaactaa tacattatcg ccttctttaa acttatcatt cgaacttgat tcaacaatac
     6421 ctgcaaaatc tataccagga atcataggat aatctctaat tacaccgcct ttatattgta
     6481 aagctagcat atccttataa tttattgaag agtataccac ttttactaat acatcaccct
     6541 tcgataaatg agattcatca actttttcta ctttataact aacatttgat gcactttttc
     6601 tcactactac cgcattgaac atttattagc cttctttcac aattgttttg tagaagtatg
     6661 tttttgttgc aaaactattt tcctacaaaa cttatcatga tatgataagc gagtcaatac
     6721 taatgattta agagaggttt tagttgtgga attagataat tgtataaatt atttgttgag
     6781 tacttctcag aatagagttt ttaaacattt taaaagttta ttaagtccct ataatattac
     6841 tccagcagaa tatggtgttt tgaattgctt gattaatagt aatttgaata cacctaaagc
     6901 gattggtaat actttaaatt tagaaccttc aacaatatct ggaatattag ataagatgtc
     6961 caaaaaggat ctaataacac gtcaaactga taatgaaaat agaagaactg tgctaataaa
     7021 cagtacaaat aaagcaaaag acttatggcc aacattagaa aagaaagcct atcaacttaa
     7081 taatcattac ttcgctaatt taactaatga agaaactgta gtgtttaaaa agatattact
     7141 aaagataaat aaaactacgt tttaattttt acgttataaa aaagttgaag tgcgaattat
     7201 aatcgagtaa atataaatga tagcttagct taattatata ttaaagccaa ctatactttt
     7261 attcccgaaa aacctactat ccaaatttct aagatacttt agcctctata ataaacatat
     7321 tgtaatataa tttatgtatt atttaggttt accctaatcg aacaataaaa aatacttgta
     7381 aaatgctatt gtcactttgc ttgatatatg agaattattt aaccttataa atgagaaaaa
     7441 agcaacgcac tttaaataag atacgttgct ttttcgattg atgaacacct ataattaaac
     7501 tattcatcta ttatttatga ttttttgtat atacaatatt tctagtttgt taaagagaat
     7561 taagaaaata aatctcgaaa ataataaagg gaaaatcagt ttttgatatc aaaattatac
     7621 atgtcaacga taatacaaaa tataatacaa actataagat gttatcagta tttattatgc
     7681 atttagaata ttaaaattcc ttcattacac tcttggcggt ttcacttatc aacttatcat
     7741 ttggcttatc acttttattg tctttattcg taaaaatgac taaaacaata ggttcagatt
     7801 ggcccttagg ataaacaaaa gcaacatcat ttctagaagc atatgttatt gcttgaccac
     7861 ttttatcagc aaccttatag tcttttggaa caccatcttt aattaaagtg tctccgtttt
     7921 tattattaaa cattaaatca agtaagaaat ttttattttt tttgcttaat tttccatttg
     7981 cgataagttt atttaaagtc ttgccgaaag cagcaggcgt tgaagtatct tttttgctct
     8041 ttggtgaata gtaatttaat tctatctcat atctaactgg atttgttact ttatctccta
     8101 gttcttttag acgttgttta acttttttga ttccaccgat ttcttttata attttattgt
     8161 ttgctgtatt atcactatat gtcattgaag cctcaataag ttcttttaaa gtgatatctt
     8221 ttcctacata tttttctaaa ataggagaat aagcaactat atcatctttg ttaatatgta
     8281 cttttttatt taacttatta taaggtactt gttctaacaa aatagcacta tttatcgctt
     8341 ttgaagtcga agcataggca aatctcttat ctgaattaaa ttttacttcc ttaccacttt
     8401 tagtatttaa agcataaaca ccaatatgag cattatattt tttttctaaa tcatttaact
     8461 ctttggcatg tgaactgttt gaattacatg cacttaaaac taaagcaatt acaattaaaa
     8521 atattaactt tttcaaaata aaccctccga tattacagtt gtaattttta taaatcaata
     8581 atattacaat tgtaatattg atgtcaattg tttattataa atatttgaaa gaaggtgtca
     8641 aaatggctaa attactaata atgagcgtag taagcttttg ttttatattt ctattgttag
     8701 tattttttag gtacatatta aaacgctatt ttaattatag tttaaattat aaagtttggt
     8761 atctaactat gcttgctgga ttaattcctt ttatcccaat taaattctct tttataaaat
     8821 ttaataatgt gaataatcaa gcgcccacag ttgaaagtaa gtcacacgat ttgaaccata
     8881 acataaatac caccaaacct attcaagagt tcacaacaga tatccataag tttaattggg
     8941 attcaattga taatatctgc acagttattt ggatagtttt agttattatt ttaagtttta
     9001 aatttttgaa agccttatta tatcttaaat atttaaagaa acagtcactt tatctaaacg
     9061 aaaatgaaaa aaataaaata gatacgatac ttttcaacca tcaatataaa aaaaatattg
     9121 tgattcgaaa agcagaggct attcaatctc caataacttt ttggtatggg aaatatatta
     9181 ttttgattcc tagttcatat tttaaaagtg taattgataa aagactaaaa tatataatac
     9241 tgcatgaata tgctcatgct aaaaatagag atactttaca tttaattatc tttaatatct
     9301 tctctattat tatgagttac aatccattag tacatattgt aaaaagaaag ataattcatg
     9361 acaatgaagt agaagccgat agatttgtcc ttaataatat taacaaaaat gaatttaaaa
     9421 cttatgcgga atctattatg gactccgtat taaaaacccc attttccaac aaaaatatat
     9481 taagccattc atttaatggt aaaaaatcat tactcaaaag tagattaatt aatataaaag
     9541 aagccgatct gaaaaagcag tcaaaattaa ttctaatttt tatttgtatt tttacttttt
     9601 ttatcatgat aattcaaagc cagtttttaa tggggcaatc cttaactgat tataattata
     9661 aaaagccctt acaaagcgat taccaaattt tagatgaaag taaaaatttt ggttcaaata
     9721 gtggatcttt cgttatgtac agcatgaaga aagacaaata ctatatttat aatgaaaaag
     9781 aaagtagaaa aagatattct cctgattcaa cttacaaaat ttatttagca ctgtttggac
     9841 ttgaccgtca tattataagt gataaaaatt cacgtatgtc atggaatcat aaacattatc
     9901 ttttcgagtc ttggaataag gaacaagatt taaatacagc aatgcaaaat tcagttaatt
     9961 ggtacttcga acgtattagt aatcaaatac caaagaacta tactgcggct caactcaagc
    10021 aattaaatta tggtaatgaa aatttgggaa gttataaaag ctattggatg gaagatagtt
    10081 tgaaaatatc taatcttgaa caagtaatag tttttaaaaa tatgatggaa caaaataacc
    10141 attttagtaa aaaagcaaag aatcaattat cttcttcatt attgattaag aaaaatgaaa
    10201 agtatgaact gtatgggaaa acaggtacag gtatagtaaa cgggaagtat aataatgggt
    10261 ggtttgtagg ttacgtaatt acaaatcatg ataagtatta ttttgctaca catttatcag
    10321 atggaaaccc gtctgggaaa aatgctgaat taataagtga aaaaatatta aaaggaatgg
    10381 gtgttttaaa tgaccaataa gcaagttgaa atatctatgg ctgaatggga tgttatgaat
    10441 ataatatggg ataaaaaatc agtatcagct aatgaaattg tagttgaaat tcaaaaatat
    10501 aaagaagtta gtgataaaac gattagaaca ttaatcacaa gactatataa aaaagagatt
    10561 ataaaacgat acaaatcaga aaatatttat ttttactcat caaatattaa agaagacgat
    10621 attaaaatga aaactgctaa aacctttctt aataaactgt atggagggga tatgaaaagt
    10681 ttagtgctga attttgcgaa aaatgaagaa ttaaataaca aagaaattga agaattgcga
    10741 gacattttaa atgatattag taaaaagtaa ttagaggtat gtttcaaaca taatacatat
    10801 gtaaaccata cttgactctt attaaattag ttttgttaat gacatttaaa atatattatt
    10861 aaagatgaga gtgtccagaa aataaccatt ttctgtacac tcttgttttt tgtctaaata
    10921 agtaagtatt acatagaatt atgtgttcaa aaacccaaca aaaaatctat gttcacaaaa
    10981 gacctcgtaa tcaagttatg gtacactaaa ggtgtatata ggaggtctgt attttgaaaa
    11041 taggttatgc aagagtttca actggattac aaaatttgaa tttacaggag gatcgattaa
    11101 atacatatgg ttgtgaaaag atatttaatg accatatgag tggttcaaaa agtaagcgac
    11161 ctggtttaga taaagcaatt gaatttgcga ggtcaggaga tacgattgtt gtttggagat
    11221 tagatagact aggacggaac atggaggatt taatcacgtt agttaatgaa cttaacgaac
    11281 gaggtgtgag ttttcatagt ttagaagaaa atataacgat ggataaatca agttctacag
    11341 gacaattatt atttcattta ttcgcagcat ttgcagagtt tgaacgtaat ttaattttag
    11401 agcgatcatc agccggtcga attgcagcac gtgctagagg ccgatatgga ggtagacctg
    11461 aaaaattaaa taaacaagat ttaaacttac ttaagacact ttatgataat ggtacgccaa
    11521 ttaaaacaat tgcagagcag tggcaagttt cacgtacaac tatttatcgt tatctcaata
    11581 aattagaggc aaaagagaat aaaaacaagt agagcaacta atctagctta ctataatgaa
    11641 tctactaaat aattttatat atcattaaca tacaaagact ttgattcaat gtcactcgat
    11701 gtaaaagaga cgttctgaca gcgtctaaaa tctcataata ttttcctgtt atgagatttt
    11761 aataataaca caagaaaaaa gcgtacaaat tcgtacgctt ttttaatata atacctgttt
    11821 cactaatgaa cgtttataac gcaatcatga tgagagtatt aatacagtta attatttgaa
    11881 atactttagt ttacataatc gatgattacc agaagttctc actatataaa agacactaaa
    11941 attttataac ttttagtgcc taatcaatta ttttagctac caaacaaacg actccaaaac
    12001 ccctttttag gttccgtttc ttcactcttt tcttctatat cactatcatt gtcatttaga
    12061 ttagctggaa cgtcttcttt ttttgcttct tcaccttgtg tactttcgtc gctttgactt
    12121 ccagatatat ccttatattg aatctctgtt tctttagttt cttcctgatc tctgttaata
    12181 tttacaggat tagtagtata tgttttttct tgtgcatcaa cgttttgtct agtgttagta
    12241 gatgtatcaa agggataatt aaggtgtttt tctttttcta attgattttc taatttttga
    12301 atctttttat tactctctaa tgccagaact tgctgatttt caagcaattt tgatctttct
    12361 tgcaactctt ttctgtaaaa ttcaattgtt gtttcatgac gactttcttg tttttcaatt
    12421 tgattattta aagtatcaat ctgattttta aggatttcta ctatttgagc atcgcttttc
    12481 gattcaaaat taatattatc cgaccctttt ttttgtttgt cttcagtagt actttcaaaa
    12541 ccatatttct ttttattttc atttactctt tgaatgattt tttctatatc ggtatcttct
    12601 ttaataaacg aagtgttttc ctgttttatt gtctctatat tcaatcgctt aatattatta
    12661 aaaatcgttt gcttactaac ccctaattct tcactcaact ttttcacact tttcatatca
    12721 tcaacccttt ataaaatgtt tataaaccct tgataaaata ttataagctt ataatgctta
    12781 ataataaagt gattaataaa taaaaataaa catccatcaa cctttttatc aagggtttat
    12841 gaaaggttta tgatatttat caagggttta taaacccttg ataaagctat attttccctt
    12901 ttataagttt atttaaagtg ctataataaa cttaatataa caaaaacccc aactactggt
    12961 aatagtcggg gtttggtaat aaaacgttta gaaactgttt atttatataa ttttcgatac
    13021 ttgtattata taatgaataa tatcttagtg caagcaccta aatcattaaa taaatgtaaa
    13081 gctaaataaa aacgttttat tacctcttac caaactaaaa tatggaaaga ggttttttgt
    13141 catgcaaaat caatatttta ca
//
