LOCUS       NZ_CP092047             2454 bp    DNA     circular CON 24-SEP-2023
DEFINITION  Enterobacter cloacae isolate AVS0889 plasmid pAVS0889-e, complete
            sequence.
ACCESSION   NZ_CP092047
VERSION     NZ_CP092047.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25692586
            Assembly: GCF_022220405.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Biggel,M., Zurfluh,K., Hoehn,S., Schmitt,K., Frei,A., Jans,C. and
            Stephan,R.
  TITLE     Complete Genome Sequence of Colistin-Resistant, mcr-10 -Harboring,
            Enterobacter cloacae Isolate AVS0889, Recovered from River Water in
            Switzerland
  JOURNAL   Microbiol Resour Announc 11 (5) (2022)
  REMARK    DOI: 10.1128/mra.00165-22
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 210.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/24/2023 08:40:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,959
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,748
            CDSs (with protein)               :: 4,748
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 24 of 96
            Pseudo Genes (incomplete)         :: 69 of 96
            Pseudo Genes (internal stop)      :: 14 of 96
            Pseudo Genes (multiple problems)  :: 10 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092047.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /isolate="AVS0889"
                     /isolation_source="river water"
                     /db_xref="taxon:550"
                     /plasmid="pAVS0889-e"
                     /geo_loc_name="Switzerland: river Lorze"
                     /collection_date="2021"
     gene            complement(join(2232..2454,1..80))
                     /locus_tag="MBA36_RS24760"
                     /old_locus_tag="MBA36_24760"
     CDS             complement(join(2232..2454,1..80))
                     /locus_tag="MBA36_RS24760"
                     /old_locus_tag="MBA36_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_023207139.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGVHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(388..501)
                     /locus_tag="MBA36_RS24765"
                     /old_locus_tag="MBA36_24765"
     CDS             complement(388..501)
                     /locus_tag="MBA36_RS24765"
                     /old_locus_tag="MBA36_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072202106.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1148..>1243
                     /locus_tag="MBA36_RS24770"
                     /old_locus_tag="MBA36_24770"
                     /pseudo=""
     CDS             1148..>1243
                     /locus_tag="MBA36_RS24770"
                     /old_locus_tag="MBA36_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(1789..1977)
                     /locus_tag="MBA36_RS24775"
                     /old_locus_tag="MBA36_24775"
     CDS             complement(1789..1977)
                     /locus_tag="MBA36_RS24775"
                     /old_locus_tag="MBA36_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024157497.1"
                     /translation="MNRQESAVLNMAKFIRAQTLLLLERIDLLDFDDEATDCEQLHEQA
                     EALYRKLSARLESDITA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          863..1035
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.379"
                     /coverage="100.0"
                     /evalue="9.47e-61"
     Typing          complement(393..938)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 gtggctccgc agtgtgggca tgactttgct gtttcgtgta tttccttgcc gcatcctcgg
       61 cagtaaacca ttgaactcat tttttatcct cgagcttatt tttagttggg atgcataata
      121 tccgatgttt tataacgata tgcatcctat atgctcgggt gggatagatt tccattatgg
      181 tgaaggatct tcttgagatc ctttctgtag cgtcgtaatc tcttgctctg taaacgaaaa
      241 aaccgcccag caaggcggtt tttcgaaggt tctcagagca gcaactcttt gaaccgtggt
      301 aactggctta tgaggagcgc aggaaccaaa tctgttcttt cagtttagcc ttaacaggcg
      361 cataacttca agactaactc ctctaaatca gttaccagtg gctgctgcca gtggcgcttt
      421 gtcgtgtctt tccgggttgg actcaagttg atagttaccg gaaaaggcgc agcagtcgga
      481 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgccttcc cggaaccgag
      541 tgtcaggcgt ggaatgagat aaacgcggcc ataacagcgg agtgacaccg gtaaaccgaa
      601 aggcaggaac aggagagcgc acgagggagc caccaggggg aaacgcctgg tatctttaag
      661 tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg
      721 gcggagccta tggaaaaacg gcttcgccgc ggccttatcg cttccccgtt aagtttcgtc
      781 ctggcatctt ccaggaaatt tctgccccgc cagtaagccg gtaccgctcg ccgcagccga
      841 acgaccgagc gcagcgagtc agtgagcgag gaagcggaac atcacctgca ttgcatattc
      901 tgcttacgct ccgctgctgc atttttctcc tgccacatga agcactttcc tgacaaccgc
      961 actcatgcca acataataag ccagtataca ctccgctagc gctacgtgac tggttcaggg
     1021 ctgcgccccg aaacccgcca aaagcctgac gcgcctgcgg cttgtccaac cccgcgccga
     1081 ccgggaaagc ttttcccgcc cgtcccggcg ttatcaggag actggtttgt ccgagaaacg
     1141 gaacaagatg ctcaccatgt gggggacgga ggatgagcac cggctactgc tggaacgctg
     1201 cgggggtaca cagctttctg catgggtgcg cccgtctgct atgtctgttc ctcctagttt
     1261 tggtgggtgc ttatatctct actaaatggc ataacatgtc attactaacc gcgatgctaa
     1321 agcgggctgt ttttcacagt attaccggcc cccgtgccgg tttttttctt accggaaggg
     1381 gcttcgtgtt tattgatgtt ctgattttcg gagggtgtac tgatcgcctt gccagggacg
     1441 cgaggaagga catatttacg acgaggtact ttctgtattt cagggaaggt gtggcagatg
     1501 agctgaccca gatatatttc tggcgtgtcg ttagtgcatt atatcccggg gcatttaacc
     1561 gtattgatta cctccctgat tctcgtaaat accgtctctg gttatcctga tttttaatct
     1621 cgtcactgct tgcttaccgt tccagtctgt attatctgca tttttctttc atactgagtg
     1681 cctcacgacc cgtaataaat tccgtgtccg ttcatccttg agcgacgact ggaggatgaa
     1741 gatctctcaa aagatgatgt ggatgccatg atgcaggcga cggaaaggct aagccgttat
     1801 gtctgattcc agtcgggcgc tgagcttccg gtaaagcgct tcggcctgct catgcagctg
     1861 ttcgcagtct gtcgcttcgt catcgaagtc gagcaggtca attctttcca gcaggaggag
     1921 tgtctgggcg cggataaatt tagccatgtt gagaacggcg gattcctggc ggttcatgtg
     1981 tgtttttccg tgtcttgtga tgattttgat gtgaaaacag gatgccgtca ttctggtgca
     2041 tagtcatgca tggcgttaaa atttacctag cacgtttctg gcggcttttc gggtggtttg
     2101 ttacctgttt taccgtacag ccgtcagaaa cgccctgaga gcctctcagg cggtgcggtg
     2161 aacggatgtt atggtaaatc caactgattg gcaggtacaa aaaaagcccc gcagggcttt
     2221 tttgctatga gttagccata ttttcttgcg aatttttcat cggaatcgca tagataaata
     2281 ataaactcaa taaacgcgat gatcgcgggt atgaatgtcc agcaaaaaat tagatacagg
     2341 aatccctgtc ctattttacc taagtaaaat ttatgtacgc caaatccacc aaggaagaat
     2401 gctaatagcg cggctgcgat tctacttttt tcacctgaac ctgaagaggc gttt
//
