LOCUS       NZ_CP092046             4307 bp    DNA     circular CON 24-SEP-2023
DEFINITION  Enterobacter cloacae isolate AVS0889 plasmid pAVS0889-d, complete
            sequence.
ACCESSION   NZ_CP092046
VERSION     NZ_CP092046.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25692586
            Assembly: GCF_022220405.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Biggel,M., Zurfluh,K., Hoehn,S., Schmitt,K., Frei,A., Jans,C. and
            Stephan,R.
  TITLE     Complete Genome Sequence of Colistin-Resistant, mcr-10 -Harboring,
            Enterobacter cloacae Isolate AVS0889, Recovered from River Water in
            Switzerland
  JOURNAL   Microbiol Resour Announc 11 (5) (2022)
  REMARK    DOI: 10.1128/mra.00165-22
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 210.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/24/2023 08:40:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,959
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,748
            CDSs (with protein)               :: 4,748
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 24 of 96
            Pseudo Genes (incomplete)         :: 69 of 96
            Pseudo Genes (internal stop)      :: 14 of 96
            Pseudo Genes (multiple problems)  :: 10 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /isolate="AVS0889"
                     /isolation_source="river water"
                     /db_xref="taxon:550"
                     /plasmid="pAVS0889-d"
                     /geo_loc_name="Switzerland: river Lorze"
                     /collection_date="2021"
     gene            392..715
                     /locus_tag="MBA36_RS24725"
                     /old_locus_tag="MBA36_24725"
     CDS             392..715
                     /locus_tag="MBA36_RS24725"
                     /old_locus_tag="MBA36_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            924..>1358
                     /locus_tag="MBA36_RS24730"
                     /old_locus_tag="MBA36_24730"
                     /pseudo=""
     CDS             924..>1358
                     /locus_tag="MBA36_RS24730"
                     /old_locus_tag="MBA36_24730"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1393..1884
                     /locus_tag="MBA36_RS24735"
                     /old_locus_tag="MBA36_24735"
     CDS             1393..1884
                     /locus_tag="MBA36_RS24735"
                     /old_locus_tag="MBA36_24735"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_238518570.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRLSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1891..2106
                     /locus_tag="MBA36_RS24740"
                     /old_locus_tag="MBA36_24740"
     CDS             1891..2106
                     /locus_tag="MBA36_RS24740"
                     /old_locus_tag="MBA36_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_064183954.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2583..3026)
                     /locus_tag="MBA36_RS24745"
                     /old_locus_tag="MBA36_24745"
     CDS             complement(2583..3026)
                     /locus_tag="MBA36_RS24745"
                     /old_locus_tag="MBA36_24745"
                     /inference="COORDINATES: protein motif:HMM:NF013561.3"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_064183953.1"
                     /translation="MIAVSLPDELLKRLDDAVDKTGKKRSFLIRESISMYLNNIENIHE
                     KKEIILNTSKPFYEVLIEEFKNEKKLITEARKTEFTMFSDNGKLYVINSKGNTRRLEPM
                     YVNNFFEEYKKTGSMSTSSYQDITFNSSYLLAALQYLMEKELI"
                     /name="ribbon-helix-helix protein, CopG family"
     gene            complement(3278..3715)
                     /locus_tag="MBA36_RS24750"
                     /old_locus_tag="MBA36_24750"
     CDS             complement(3278..3715)
                     /locus_tag="MBA36_RS24750"
                     /old_locus_tag="MBA36_24750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064183952.1"
                     /translation="MQQAIRMRIEADKAALDAGFTTNNFVGELGEKLAHAVYGGRILPN
                     SAKSADIQTDDGTLLQVKTRKPNKGKQFGSIRSWDFDYLIAITLGDKYEVIRALKIPVS
                     TCKELADHRAHTNSFIIKPTRKLLSHADIEDVTDKFQSVVL"
                     /name="hypothetical protein"
     gene            complement(3944..4057)
                     /locus_tag="MBA36_RS24755"
                     /old_locus_tag="MBA36_24755"
                     /pseudo=""
     CDS             complement(3944..4057)
                     /locus_tag="MBA36_RS24755"
                     /old_locus_tag="MBA36_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          105..288
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.583"
                     /coverage="86.0"
                     /evalue="2.62e-72"
     Typing          complement(3941..4307)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.954"
                     /coverage="85.0"
                     /evalue="8.32e-150"
     Typing          393..709
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="3.23e-61"
     Typing          706..2198
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcctttttc cggcccttcc tgacttgagt actgcttgcg tgtcccgtca tctttgagct
       61 ggtaacgccc gccgcagtct cacggtagga gcgtagcgac cgagtgagcg aggaggcgta
      121 atttcatcct tatctgcatg ttgcacttac gcccggtagc ctcatttttc cccagccaca
      181 tgaagcactt ccatacatta ccactccgcc gtaacatatt aagccagtat acactccgct
      241 agcgctacgt gactggttca gggctgcgcc ccgaaacccg ctaaaaccac tgacgcgcct
      301 gcggcttgtc caacaccgcg ccgaccggga aaggctttcc cgcccgtccc ggcgttatca
      361 ggaggccggt ttgtcagaga aacggaacaa gatgctcacc atgtgggtga cggaggacga
      421 gcaccggcgg ctgcttgagc gctgtgacgg caggcagctg gcggcgtgga tgcggcagac
      481 ctgcctggac gaaaagcctg cgcgcaccgg aaagctcccc tcaatctcgc cggcgctgct
      541 gcgccagctt gccggcatgg gcaataacct gaaccagata gcccgccgtg ttaacgccgg
      601 cggcggcacc ggccatgaca gggtgcagat tgtggcggcg ctgatggcca tcgatgccgg
      661 gcttgagcgg ctgcggcacg ccgttcttga gaaaggaacg gacgatgatc gttaaatttc
      721 atccacgcgg tcgcggcggt ggcgccgggc cggtggacta tctgctgggt aaagatcggc
      781 agcgtgaagg cgccagcgtc ctgcagggga agccagaaga agtccgggag ctgattgacg
      841 cgtcgcctta cgtgaagaaa tacacctccg gcgtcctgtc gtttgcggag gccgatctgc
      901 ctcccggcca gcgggaaaaa ctgatggcca gcttcgagcg ggttctgatg cccggacttg
      961 ataaagatca gtacagcatt ctgtgggtgg agcacgagga caaggggcgt ctggagctga
     1021 actttctgat ccccaatacc gagctgctga ccggcaggcg gctccagccg tactacgacc
     1081 gcgccgaccg gccgcgtatc gatgcctggc agaccatcgt gaacggcaga ctggggctgc
     1141 acgacccgaa cgcaccggaa aaccgccggg cgctgatcac gccgtccggg ctgccgaaaa
     1201 cgacgcagga ggccgcagaa gccatcacgc gcggactgct gacccttgct tcgtccgggg
     1261 agctgaaaac ccgtcaggac gtcactgagg cgctggaacg gtcaggtttt gaggtggtgc
     1321 gcaccacgaa aagcagtatc agcattgccg acccggacgg ggggcgaaac atccgactga
     1381 agggagccat ctatgagcag tcttttaacg ctggcgaagg acttagagca gcaatcgaaa
     1441 gcgcagcagc agagtaccgg cgagatgctg aaagccgcat tcagcgagca cgagcagtct
     1501 gtcagagcgg aactgagcgc aagcgcgagg agaatcagcg acgccatcct cgcacacgag
     1561 cagagtatga gcgaggcgat ggagaaaaac cggctgagcg tactgcggac tgccgggcgg
     1621 acatggctca ccatcctgat ggtgtcagcg ctactgatcg gcacgagcgg gagcattctg
     1681 tggtggcagg ggcagcagat aaccgacaat tacacgcatc ttcgtcagca ggaagatacc
     1741 ctggcgaaaa tgaccgcccg gacgtgggga gtgcgctatc aggagagcag cgacggccgg
     1801 aggtttctta tcctgccgcc ggggatgcaa accgaagcca tcccttacga cgggacgaca
     1861 tggatccgcc tgaaacagga gtagcggaaa atgacagagc tggaaaagca gttgctgagc
     1921 gcattagagc agctacagca ggactactcg caaaggctgg acgaatggga gagcgccttc
     1981 gcggaatggc ggagcatgtg tggtcttatg caacgggaga acgcggtgct gaacgagcgc
     2041 gtgtcggact tgagcacgca ggtgctgagt ttaagcgagc agctgcgccg cttgtccggg
     2101 agctgagtac ggtagaacag cggcagcggg agcatgagta tcagaaaagc ctggagttgc
     2161 agcgccagcc gcagaaaacc tacaaaggac cgacgctggg ctgacggatg ccgtcccgtg
     2221 acaaacccca agaagaaggg ctgcgaaatt ccccgctaaa tacgtcgtga tgcaaagatc
     2281 acggcctata ccagggggaa ttatgaaact gcttaaggtt attgaagctg tacttaactg
     2341 cctgcatctg gcggcagaac tgtgggagtg gctggagaag catttgccgg ttttcggggc
     2401 cttcacgctg tttttgggct gaatcctgcc gtcattcacc tggctgcatg gctatgcagt
     2461 tgggtaaaat ttatctgtcg cgatcggggt ggtttaccgg gtgggttgtt gttggttttg
     2521 gctgactgcc gccccgttcg tggcgaagct gtccggggcg gtgcgggcaa tgagtgctta
     2581 tgttaaatca gttctttttc catcaggtac tgtagtgcag ctaaaagata agatgagttg
     2641 aaggttatat cttgatagct agatgtactc atacttcctg tctttttata ctcttcaaag
     2701 aaattattca catacatggg ttctagcctg cgcgtattcc ctttcgaatt tatgacatag
     2761 agttttccgt tatcactaaa catcgtgaac tcagtttttc tggcctcagt tattagtttt
     2821 ttttcatttt taaattcttc aatgaggacc tcatagaacg gttttgaggt gttcaggatg
     2881 atctcttttt tttcatgtat gttttctatg ttattcaaat acatagatat tgattctcgt
     2941 atcaagaatg agcgtttttt accagtttta tctacagcat catctagtct ttttagtaac
     3001 tcgtctggaa gtgatacagc aatcatgaac agccctctaa atttataatc taattaacta
     3061 atctaaattt atataaaaat agagcggccg tcaatagtcg agatttcatt ttctaatcag
     3121 ttgcatgttc tgattagttc gtaggtacgt gcaagggatg ttatggtaaa agacggtaaa
     3181 gccggatgct tgtgcaatag cgcccttcta ctcaagatgg cggttttttt acggtcgtga
     3241 tttcgtgcag aattattcaa gtagggggta aaaccaccta taaaacaaca ctctgaaatt
     3301 tatccgtcac atcttcaata tccgcatgtg aaagtaattt tctggtcggc tttattatga
     3361 aagagttcgt atgtgcccga tggtcggcca gttctttaca ggtagatact gggattttta
     3421 aggccctgat gacttcgtat ttatcaccca gcgttatcgc tatcagataa tcaaaatccc
     3481 aagagcggat gctaccaaac tgttttcctt tgttcggctt cctcgttttc acctggagta
     3541 aagtgccgtc gtctgtctgt atgtctgcgc ttttagctga gttaggaagt atgcgacctc
     3601 catatacggc atgagctaat ttttcgccca gttcaccgac aaaattattc gtagtaaaac
     3661 cagcatctaa tgctgcttta tcggcttcga ttctcatacg gatggcttgt tgcatacttt
     3721 ttgtagccct taatggggga agatcttctt gagttccttt ttttctgcgc gtaatctctt
     3781 gctttgaaaa cgaaaaaacc gcccagcaag gcggtttttc gaaggttctc agagcagcaa
     3841 ctctttgaac caaggtaact ggcttggagg agcgcagata ccaaaactgt cctttcagtg
     3901 tagccttatc gcacccgaaa cttcaagact ctcctcctct aaatcagtta ccaatggcta
     3961 ccgccactgg cgattttttg tgtctgtaag ggttggactc aagacgatag ttaccttaca
     4021 tggcgcggta gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     4081 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
     4141 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagctacc agggggaaac
     4201 gcctgggatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcatatttcg
     4261 tgatgcttgt caggggggcg gagcctatgg aaaaacggca acgccgc
//
