LOCUS       NZ_CP092045             4783 bp    DNA     circular CON 24-SEP-2023
DEFINITION  Enterobacter cloacae isolate AVS0889 plasmid pAVS0889-c, complete
            sequence.
ACCESSION   NZ_CP092045
VERSION     NZ_CP092045.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25692586
            Assembly: GCF_022220405.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Biggel,M., Zurfluh,K., Hoehn,S., Schmitt,K., Frei,A., Jans,C. and
            Stephan,R.
  TITLE     Complete Genome Sequence of Colistin-Resistant, mcr-10 -Harboring,
            Enterobacter cloacae Isolate AVS0889, Recovered from River Water in
            Switzerland
  JOURNAL   Microbiol Resour Announc 11 (5) (2022)
  REMARK    DOI: 10.1128/mra.00165-22
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 210.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/24/2023 08:40:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,959
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,748
            CDSs (with protein)               :: 4,748
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 24 of 96
            Pseudo Genes (incomplete)         :: 69 of 96
            Pseudo Genes (internal stop)      :: 14 of 96
            Pseudo Genes (multiple problems)  :: 10 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP092045.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /isolate="AVS0889"
                     /isolation_source="river water"
                     /db_xref="taxon:550"
                     /plasmid="pAVS0889-c"
                     /geo_loc_name="Switzerland: river Lorze"
                     /collection_date="2021"
     gene            join(4612..4783,1..71)
                     /locus_tag="MBA36_RS24690"
                     /old_locus_tag="MBA36_24690"
     CDS             join(4612..4783,1..71)
                     /locus_tag="MBA36_RS24690"
                     /old_locus_tag="MBA36_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(742..957)
                     /locus_tag="MBA36_RS24695"
                     /old_locus_tag="MBA36_24695"
     CDS             complement(742..957)
                     /locus_tag="MBA36_RS24695"
                     /old_locus_tag="MBA36_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(964..1455)
                     /locus_tag="MBA36_RS24700"
                     /old_locus_tag="MBA36_24700"
     CDS             complement(964..1455)
                     /locus_tag="MBA36_RS24700"
                     /old_locus_tag="MBA36_24700"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<1490..2194)
                     /locus_tag="MBA36_RS24815"
                     /pseudo=""
     CDS             complement(<1490..2194)
                     /locus_tag="MBA36_RS24815"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2133..2456)
                     /locus_tag="MBA36_RS24710"
                     /old_locus_tag="MBA36_24710"
     CDS             complement(2133..2456)
                     /locus_tag="MBA36_RS24710"
                     /old_locus_tag="MBA36_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3107..>3229
                     /locus_tag="MBA36_RS24820"
                     /pseudo=""
     CDS             3107..>3229
                     /locus_tag="MBA36_RS24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3549..3905)
                     /locus_tag="MBA36_RS24715"
                     /old_locus_tag="MBA36_24715"
     CDS             complement(3549..3905)
                     /locus_tag="MBA36_RS24715"
                     /old_locus_tag="MBA36_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(4108..4425)
                     /locus_tag="MBA36_RS24720"
                     /old_locus_tag="MBA36_24720"
     CDS             complement(4108..4425)
                     /locus_tag="MBA36_RS24720"
                     /old_locus_tag="MBA36_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2572..2745)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          2669..3216
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.559"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(651..2143)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2140..2456)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
ORIGIN
        1 cacaaaacca ccaccaacat acccccgcgc aagggaacat tcgttggcaa ttgtttgttt
       61 atgctcagtg aggtatagaa aatggcagat cgtcgaccac tcaacccaca cgttgtcgtt
      121 cctaaaccag gcggcggaac tcaacctcgt gaccgtaaca atgacggaag ggtcagagga
      181 aaacgctcag atgcaggaaa gccacgaaaa taacagcaag ccgccagcct ggcggctttt
      241 ttatatctgc agctgatact gatttaccat aacatccgtt gggcgcaccg ctgaaacggg
      301 gttggctgcg tttccggtgc cagacgcgag aaagtggcga cagacataac gaaaaaacac
      361 cagaaacgca gccgctgaat attacactgg tgcatagtca tgcagccaga tgcatggatg
      421 ttaaagtcac actaaaatcc gcgccaatgc cttaaaaacc gacaaatgct tctccagcca
      481 gtcccacagc tccgccgcca gatacaggca gttaagcaat gattcaatga gatttaacag
      541 cttcatttcc cgctcctgaa taagtcgtga cctttttgat cacgaaccta tttagcggga
      601 gaaataagag cccctcttcg tgcgggttgt cagctgcctg gatggtttaa agtgtcggcc
      661 cgcggtaggt tttctgcggc tggcgctcca gctccagtgt tttctgatac tcattctccc
      721 tctgccggag ctcgatggca ttcagttccc ggacaagcgg cgcagctgct cgcttaaact
      781 cagcacctgc gtgctcaagt ccgacacgcg ctcgttcagc accgcgttct cccgttgcat
      841 aagcccacac atgctccgcc attccgcgaa ggcgctctcc cattcgtcca gcctttgcga
      901 gtagtcctgc tgtagctgct ctaatgcgct cagcaactgc ttttccagct ctgtcatttt
      961 ccgctactcc tgtttcaggc ggatccatgt cgtcccgtcg taggggatgg cttcggtttg
     1021 catcccgggc ggcaggataa gaaacctccg gccgtcgctg ctctcctgat agcgcactcc
     1081 ccacgtccgg gcagtcattt tcgccagggt atcttcctgc tgacgaagat gcgtgtaatt
     1141 gtcggttatc tgctgcccct gccaccacag tatgctcccg ctcgtgccga tcagcagcgc
     1201 tgacaccatc aggatggtga accatgtccg cccggcagtc cgcagtacgc tccgccggtt
     1261 tttctccatc gcctcgctca tactctgctc gtgtgcgagg atggcgtcgc tgattctcct
     1321 cgcgcttgcg ctcagttccg ctctgacaga ctgctcgtgc tcgctgaatg cggctttcag
     1381 catctcgccg gtactctgct gctgcgcttt cgattgctgc tctaagtcct tcgccagcgt
     1441 taaaagactg ctcatagatg gctcccttca gtcggatgtt tcgccccccg tccgggtcgg
     1501 caatgctgat actgcttttc gtggtgcgca ccacctcaaa acctgaccgt tccagcgcct
     1561 cagtgacgtc ctgacggttt ttcagctccc cggacgaagc aagggtcagc agtccgcgcg
     1621 tgatggcttc agtggcctcc tgcttcgttt tcggcagtcc ggacggcgtg accagcgccc
     1681 ggcggttttc cggcgcgttc gggtcatgca gccccagtct gccgttcacg atggtctgcc
     1741 aggcatcgat gcgcggccgg tcggcgcggt cgtagtacgg ctggagccgc ctgccggtca
     1801 gcagttcggt attggggatc agaaagttca gctccagacg ccccttgtcc gcgtgctcca
     1861 cccacagaat gctgtactga tctttatcga gtccgggcat cagaacccgc tcgaagctgg
     1921 ccatcagttt ttcccgctgg ccgggaggca gatcggcctc cgcaaacgac agtacgccgg
     1981 aggtgtattt cttcacgtaa ggcgacgcgt caatcagctc ccggacttct tccggcttcc
     2041 cctgcaggac gctggcgcct tcacgctgcc ggtctttacc cagcagatag tccaccggcc
     2101 cggcgccacc gccgcgaccg cgtggatgaa atttaacgat catcgtccgt tcctttctca
     2161 agaacggcgt gccgcagccg ctcaagcccg gcatcgatgg ccatcagcgc cgccacaatc
     2221 tgtaccctgt catggccggt gccgccgccg gcgttaacac ggcgggctat ctggttcagg
     2281 ttattgccca taccggcaag ctggcgcagc agcgccggcg agagtgaggg gagctttccg
     2341 gtgcgcgcag gcttttcgtc caggcaggtc tgccgcatcc acgccgccag cggcctgccg
     2401 tcgcagcgct caagcagccg ccggtgctcg ccctccgtca cccacatggt gagcatcttg
     2461 ttccgtttct ctgacaaacc ggcctcctga taacgccggg acgggcggga aagcctttcc
     2521 cggtcggcgc ggggttggac aagccgcagg cgcgtcagtg gcttttggcg ggtttcgggg
     2581 cgcagccctg aaccagtcac gtagcgctag cggagtgtat actggcttaa tatgttggca
     2641 cggatacgga aatcagtgaa gtgcttcatg tggcaggaga aaaaaggcgg caacggcgcg
     2701 tcagcagaat atgcgataca ggatatattc cgcttcctcg ctcactgact cgctgcgctc
     2761 ggtcgttcgg ctgcggcgag cggtgcgggc ttacgaacgg ggcggaaatt tcctggaaga
     2821 tgccaggaag aaacttaaca gggaagcgat aaggccacgg cagagccggt tttccatagg
     2881 ctccgccccc ctgacgagca tcacagaatc tgacgctcaa atcagtggtg gcgaaacccg
     2941 acaggactat aaagatacca ggcgtttccc cctgatggct ccctcgtgcg ctctcctgtt
     3001 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     3061 cctgacactc ggttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     3121 cgttcagtcc gaccgctgcg cctgttccgg taactatcga cttgagtcca acccggaaag
     3181 acacgacaaa acgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3241 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacactg gaaggacagt
     3301 atttggtatc tgcgctctgc tgaagccagt taccaggtta agcagttccc caactgactt
     3361 aaccttcgaa aaaccgcctc tccaggcggt tttttcgttt acagagcaag agattacgcg
     3421 tacagagcac ggatctcaag aagatcgtaa gaagaaagtt aacatgagcg tttttatccc
     3481 tcaaaaagaa ggcttctagt tatagaagcc ttctttttga gggataactt atattttttt
     3541 aaaaaaaatc atgcaatagc atgtttcttc tcagaactat caacaatgtg tttcctttct
     3601 atgatttcat gagcgggaat cactctgtct atttcttctt gcaatcgagg gctactagta
     3661 acctcccata aatcaacaga ctgctgtata tttagccaga actttgcagt ggtatcgaag
     3721 gctttagcca acctaaaagc catatcggta gacagctttc tattattgtt aactaaagca
     3781 ctggcagagt ttctatgcac attcaacatt tcagccaagt cattgatttt taaaccactt
     3841 ggttcgagat attcataaag aataatatcc cctacagttg atggttttct agttgcttgg
     3901 gacatgcttt actcctaaat tttttacttc tctctgttag atgaggaaga taagtatttc
     3961 gtctattacc agctgtagcg atgagtaatt atcttaaata tagtcacgtc atttttttta
     4021 ttaatcatta tttgtaatga cattttaatg taggtgaggg aacgcctcac ctacttgttt
     4081 tataagataa cagccacgac ctgttatcta tcggtaatca tgtggatcga gatacaaatc
     4141 ctcgactccg ttttcacccc acttaaatat caagcggtac ttctcattca cccgtatgga
     4201 tgaataccct gtcaatggag ggttgagatt ctcaaatcgg tttcccgggg gagaccgcaa
     4261 atctccaact ttatcagcag cattcagcat atccagcttg cgacctaatg aagcactaat
     4321 cgcagttgga atctttcgat tggctttacc atatttgtaa aactggtata gccaatcgtc
     4381 tcgaaactga atgcggcaat catcatgttc atgatcttgc tgcatttttc ttttacctca
     4441 atttttgccg caacttggca attttttcga tcaccttctt acctccgata tagtacagca
     4501 aacatatcaa atgcacaaca gcacagtgca cttgtgcaat caatggctat gctgtagaat
     4561 gcactcatgc acagtgcagg tgtgcaaaat aaatgataat ttggaggttg catggctctt
     4621 gttcgctcag gaaacaaaag caagattttt gaagcagata cgaagtttaa ctcggttttt
     4681 gacactgaat ataagccggg agaaacatgt gaatatgaag ggatttataa atgtaccggt
     4741 tgcggtaaag aagtaactat cgcaggcggg aaaacaatcc cac
//
