LOCUS       NZ_CP091991             1901 bp    DNA     circular CON 14-FEB-2022
DEFINITION  Klebsiella pneumoniae strain NB04 plasmid pNB04-6, complete
            sequence.
ACCESSION   NZ_CP091991
VERSION     NZ_CP091991.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25688211
            Assembly: GCF_022213405.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Huang,J. and Li,X.
  TITLE     Whole genome sequence of NDM-1 and DHA-1 co-producing Klebsiella
            pneumoniae from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Huang,J. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue Street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2022 14:16:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,635
            Genes (coding)                    :: 5,496
            CDSs (with protein)               :: 5,496
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 47 of 139
            Pseudo Genes (incomplete)         :: 94 of 139
            Pseudo Genes (internal stop)      :: 14 of 139
            Pseudo Genes (multiple problems)  :: 15 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NB04"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pNB04-6"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2021-06-11"
     gene            252..800
                     /locus_tag="MA881_RS02865"
     CDS             252..800
                     /locus_tag="MA881_RS02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_106642455.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKAG
                     WNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFESSLKYSNHDSEQGDAL"
                     /name="replication/maintenance protein RepL"
     gene            797..1084
                     /locus_tag="MA881_RS02870"
     CDS             797..1084
                     /locus_tag="MA881_RS02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049092722.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIEYATGINLYMNCED
                     KEFNLVDVINEDGNKIMFVVSNDEKIQKPKKIAKKKTPAPVIELDKYRKK"
                     /name="hypothetical protein"
     Typing          complement(1321..1581)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.237"
                     /coverage="100.0"
                     /evalue="3.82e-131"
ORIGIN
        1 tgactgggtc agggctgcgc cccgacaccc caaaagcagt taggtagtgt taaatcatga
       61 gcagtaaaaa aacgctttga tagatcttta gagcaaaaat tgacatttct ttaaaaatca
      121 ttgacttaag acaccatcat acgaaacgtt atttgcatgt gtatacgcaa acatgtttgc
      181 atgtgttgtt tcgtactgta cgatataaat ttcgtaaggt gcgaaatatc attgcaggta
      241 aggatgcaag tatgttggaa cagcaatttg aagactttga acagcaaaaa cggcgagatc
      301 atcaggtaca agatcaatcc aagaacgcta agcaaaaacg agatcggttg aatcctccag
      361 actggtcgca ggttactaag gctggttgga atatgttgga cgctatatca gaaaaagttc
      421 ctagagcatt tcggttatat tctttttttg cccagaatat agataaatca tgtggggctg
      481 ttgtatgtga tcaaaaattc ttagctgatt atttcggtgt tagtttaaga acgatccaat
      541 attggattgg ggatttagaa agtattggcg caatagttaa aataccagta ggcatatatt
      601 atgcctatgc attagatcct tatcaggtat ggcgtggata taacaatact aaaaaatatg
      661 cagcatttaa cgcaaaaaca ttaactcaaa aagatggggt tatagatcgt aaattgcggc
      721 ttatgctttc taggcgtgaa gagtttgaaa gttccttaaa gtactctaat catgactcag
      781 agcagggtga tgcactatga gaattaaata tgaatcacgt tggagattag aagaactaaa
      841 attagaaata tgcagttcaa tagatgagtt aattaataaa tatggtattg aatatgcaac
      901 aggaataaat ctttatatga attgtgaaga taaagaattt aatcttgttg atgttatcaa
      961 tgaagatgga aataaaataa tgtttgtcgt tagcaacgat gaaaaaatac aaaagccaaa
     1021 aaaaatagca aagaaaaaaa ctccagcacc tgtaattgag ctagataaat atcgaaaaaa
     1081 atgaccattg agccagcggc aggcggacaa tcaggggcta cgtgttaacg ttctgaccat
     1141 gattgtctat cctgcattgc tcttttgccg cctcgaatcc cttgcgtgtt tttgttcccc
     1201 gctctccaga acgaccgagc cgccacggtt ccgatagcgc cttgagcgca accggaaacc
     1261 gtctggggag aaccccagac ccccatcgct gtaacaccaa tggtctacga ccacctacca
     1321 accgaatagc tgcctgtgcc gtgcaaatag gctttgcagg gggctgacgc tgttcctagc
     1381 gacctgaaag cccctggtta ggggtatcgg cgcgcctgac ctcctgaaac gctctgtaag
     1441 cgtttttcgc gcctcccatg ccctgacggg catttaagac ccgtttagtg cgtctgagag
     1501 cttctgagcg cgtttgaggg gctatctgac gactcatccc tgaaagaaca acaacgggat
     1561 caccgacaac atgcgagagg tgataccgct cgccgcaagt cgatgaccga gcgtagcgag
     1621 cgaatcgacg aggaagcgga agagaaccgg aagccacatt gagcacttac gcactgatgc
     1681 ggggtgtcgg ggtgaagccc tgaccaggtg gcatttgtct gattgcgtgt gcgcggtccg
     1741 acaaatgcac atcctgtccc gatttctgag gctttttagc ggtgaacgga cggcaaaacc
     1801 atgatggagt tgttgaacgt tgagagcatg aaatctggca aaaatcacgg ctggtaaggc
     1861 ttgcagagga gtaagccagt atacactccg ctatcgctac g
//
