LOCUS       NZ_CP091990             2089 bp    DNA     circular CON 14-FEB-2022
DEFINITION  Klebsiella pneumoniae strain NB04 plasmid pNB04-5, complete
            sequence.
ACCESSION   NZ_CP091990
VERSION     NZ_CP091990.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25688211
            Assembly: GCF_022213405.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Huang,J. and Li,X.
  TITLE     Whole genome sequence of NDM-1 and DHA-1 co-producing Klebsiella
            pneumoniae from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Huang,J. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue Street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2022 14:16:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,635
            Genes (coding)                    :: 5,496
            CDSs (with protein)               :: 5,496
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 47 of 139
            Pseudo Genes (incomplete)         :: 94 of 139
            Pseudo Genes (internal stop)      :: 14 of 139
            Pseudo Genes (multiple problems)  :: 15 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091990.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NB04"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pNB04-5"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2021-06-11"
     gene            join(2045..2089,1..921)
                     /locus_tag="MA881_RS02855"
     CDS             join(2045..2089,1..921)
                     /locus_tag="MA881_RS02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            925..1191
                     /locus_tag="MA881_RS02860"
     CDS             925..1191
                     /locus_tag="MA881_RS02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          350..581
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gtttgggata cccagcgcac gcttaccgat tctgtcgggg gtatctacca gactgctgct
       61 gaattcgagc gctatgcact ccgtatggcc tcctgtagcg gtttgttacg ttttggttgg
      121 tctaccatca tggaaaccgg agaaacgcgc ctacggcttc gtagtgcgca attttgccgt
      181 gtccgtcatt gccctgtctg ccagtggaga agaaccctca tgtggcaagc ccgtttttat
      241 caggctctac cgaaaatcgt tgtggattac ccgtcttccc gatggttgtt tctgacgtta
      301 actgtcagga actgcgagat aggtgaactt ggaacagtcc ttacagcaat gaatgcggcg
      361 tttaagcgaa tggaaaagcg aaaggagcta tcacctgttc aggggtggat cagggctacg
      421 gaggtgacgc gaggtaagga tggcagcgca catccgcatt ttcactgtct gctgatggtg
      481 caaccttctt ggtttaaagg gaagaactac gttaagcacg aacgttgggt agaactctgg
      541 cgcgattgct tgcgggtgaa ctatgagccg aatatcgata ttcgggcagt aaaaactaag
      601 acaggtgagg ttgtggccaa cgttgccgag caactgcaaa gcgcggttgc tgaaacgctg
      661 aaatactccg ttaaaccgga agatatggca aacgatcctg agtggtttct tgagctgacg
      721 cggcagcttc acaagcgccg ttttatctcg accggtgggg cgctaaaaaa cgtcctccag
      781 ttggatcgag aaaccaatga ggatcttgtc attgccgacg atgtagggga tggcactgat
      841 gacgggaagc ggacggcgtt tgtctgggat tcaggtaaac ggcgttacaa acgcgcccct
      901 gagaaggata aatcggatta acgtatgaat attaatattg aatacctgaa tggaaataag
      961 actattggtt tatttttttt aagaagtgaa gcggtgattc ctgacaggtt taaaaacctt
     1021 attttgctta ttgatggatt aagttttggc acatttggtt ttcatccgca cgaaggtttt
     1081 gaggatgaat taattttata tattcagaaa acaaacgaga gggtaaaaac tctttttgtg
     1141 aaaatagatt taaacggtat caagtttgga tttttaagaa cgcattctta gttctggaaa
     1201 agagccagcg gcaggctgag gtgataggta cgagattgca tgcaatctct agtgctctgt
     1261 ctatcctgca ttatcctcag cattatcctc agccttgcca actcgacacc aatgcaggat
     1321 agacaatccg atgtcaaatg ttaacactct gcgagtggta cattttcccc ggattatcgt
     1381 cctgagcctg ccgctggctc tctttctacc gcctcgcttt gctcgttgct caacgcctca
     1441 cagacacgga ttaaaatccg catccgttca ccgtttttta aagtccgtta aaagcatgat
     1501 gccatctccg agagttaatc tcgtcaaatg ctaaatcgtg ggggtcccct ttggggttcc
     1561 gatttagtga ttgacgacac caccgattaa aaaacttatg cggggtggat ggtttcacga
     1621 agtgaggcca tccacctgta agacagggtt ttgtttttat tccctgtttt ggtgatcggg
     1681 tgtgtggaaa aggttggggt aagccgttcg ggggtgcttg ttttgggggg ttaaagttgt
     1741 ggttattttt tgcgcaattc tcgcgcgtga tccttgtatt tatacttaag ggataaatgg
     1801 cggatatgaa atagtggttt agcccagtaa tgacgaggct ttgagtgggt tttgacaggt
     1861 caaagaaaat ggagcagaat tgaggcgttt ttaatcggcg ttggggagtg cgtcaacact
     1921 ccccaacatt tcgaatgtgt cacctcagcg gcaaactctg gtgacatgta ctggctcgca
     1981 atgcacaggt acgtgatgaa tataccacat caaatcacag cctgcccaga tcggagcagg
     2041 cttaatgtca gaagataaat tcctttcgga ctacagcccc cgtgatgca
//
