LOCUS       NZ_CP091989             4510 bp    DNA     circular CON 14-FEB-2022
DEFINITION  Klebsiella pneumoniae strain NB04 plasmid pNB04-4, complete
            sequence.
ACCESSION   NZ_CP091989
VERSION     NZ_CP091989.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25688211
            Assembly: GCF_022213405.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Huang,J. and Li,X.
  TITLE     Whole genome sequence of NDM-1 and DHA-1 co-producing Klebsiella
            pneumoniae from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Huang,J. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue Street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2022 14:16:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,635
            Genes (coding)                    :: 5,496
            CDSs (with protein)               :: 5,496
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 47 of 139
            Pseudo Genes (incomplete)         :: 94 of 139
            Pseudo Genes (internal stop)      :: 14 of 139
            Pseudo Genes (multiple problems)  :: 15 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091989.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NB04"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pNB04-4"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2021-06-11"
     gene            complement(join(4494..4510,1..175))
                     /locus_tag="MA881_RS02825"
     CDS             complement(join(4494..4510,1..175))
                     /locus_tag="MA881_RS02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1004..1360)
                     /locus_tag="MA881_RS02830"
     CDS             complement(1004..1360)
                     /locus_tag="MA881_RS02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(1564..1884)
                     /locus_tag="MA881_RS02835"
     CDS             complement(1564..1884)
                     /locus_tag="MA881_RS02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2067..2309
                     /locus_tag="MA881_RS02840"
     CDS             2067..2309
                     /locus_tag="MA881_RS02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            complement(2851..3297)
                     /locus_tag="MA881_RS02845"
     CDS             complement(2851..3297)
                     /locus_tag="MA881_RS02845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(4084..4494)
                     /locus_tag="MA881_RS02850"
     CDS             complement(4084..4494)
                     /locus_tag="MA881_RS02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          295..704
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attcatccag tctcgaggac agtgtacgga aaagatgctc cgcatcatcg tggagtttct
       61 cacaaaggtc ggcctctgca tcaagatcaa gctcgttaag cttctccagc aggagcagag
      121 actgattctg gatgaattta gccatgttca gcgctgtttt ctgttgtttc gtcataagaa
      181 gcctcagagc gtcagtgctc agtgtaaaac cagtctcttt tccgcttcct cgctcactca
      241 gtcgctacgc tcctgccgtt cggctgcggc gagcggtagg actatgcgta taaatgctcg
      301 agaaaaccga gcggacgcgt gatgggcaga aggaaaggcc gcttagcggc cgtttttcca
      361 taggctccgc ccccctgaca agcatcatag aaattgacgc tcaaatcagt ggtggcgaaa
      421 cccgacagga ctataaagat atcaggcgtt tccccctgat ggctccctcg tgcgctctcc
      481 tgttcctgcc attcggttta ccggtgtcat tccgctgtta tggccgcgtt tatctcattc
      541 cacgccagac actcagttcc gggaaggcag ttcgctccaa gctggactgt atgcacgaac
      601 cccccgttca gtccgactgc tgcgtcttat ccggtaacta tcgtcttgag tccaacccgg
      661 taagacatgc aaaagcgcca ctggcagcag ccactggtaa ctgatttaga ggagttagtc
      721 ttgaagtcat gcgcctgtta aggctaaact gaaagaacaa gttttggtga ctgcgctcct
      781 ccaagccagt taccttggtt caatcacctg caagtgtttg aaccttcgga aaaaccgccc
      841 tgcaaggcgg tttttccgtt tacagagcaa gagattacgc gtaccgaaac cggatctcaa
      901 gtgctcgtaa aagaaaatga tgatgacctt tagcggttac aaaaaaagaa ggcacactat
      961 tacagaggcc ttcttctagt ttggttaaat ttttttaaaa aaatcatgcg accgcatgtt
     1021 ttttttccaa agattcagca ttgtgcttcc tttctataat ttcatgagcg ggagtgactc
     1081 tttctatttc ttcctgaagg cgtgggctgc tcgtaacctc ccacaaatca acagactgct
     1141 gtatatttaa ccagaactta gcggtagtat cgaatgcttt agctaaccga aacgccatat
     1201 cggtagacag tttcctatta ttgttaacca aagcacttgc agaatttcta tgcacgttta
     1261 acatttcagc caagtcattg atttttaaac cacttggctc gagatattca taaagaataa
     1321 tatctcctac agtcgatggt tttctggttg cttgggacat gctttactcc tgaattggtc
     1381 gcttctctct gttagatgag gaagataagt atttcttctg ttaccagctg tatcgattag
     1441 taattatctt aaatatagtt acgtcgcgtt actttattaa tcattatttg tgatgacgtt
     1501 ttaacgtagg tgaggaaatg cctcacctac ttgttttgta agataacagt tccggtctgt
     1561 tacctatcgg taatcatgtg gatcgagata caaatcctcg actccgttct caccccactt
     1621 aaatatcaag cggtacttat cattcaccct tatagatgaa taccctgtca atggagggtt
     1681 gagattttca aatcggttcc ccgggggaga ccgtaaatct ccaattttat cagcagcatt
     1741 cagcatatcc agcttgcgac ctaatgaaac actaatcgta gctggaattt ttcgattggc
     1801 tttaccgtat ttataaaact ggtatagcca atcgtctcgg aactgaatgc ggcaatcatc
     1861 atcactatca tgattttgct gcatttttct tttacctcaa atgttgccgc aacttggcaa
     1921 gttctagatc accttttacc accaccatag tacaacaaac gccagaaatg cacaatagca
     1981 ctgtgcactt gtgcaaaaaa ccataatgat ataaaatgca caaaagcaca gtgctactgt
     2041 gcaaaaaaag aatagttgga ggtcatatgg cgcttgttcg ctcaggaaat aaaaccaaga
     2101 tttttgaagc agatccgaaa tttaacccag tttttgatac acaacacaag cctggcgata
     2161 cgtgcacgta tgaaggaatt tacaaatgca caggttgcgg taaagaagta actatcgcag
     2221 gagggaaaac acttccacca caaaatcatc atcaacatag tcccggacag ggggacatcc
     2281 gctggcaact atttgtttat gcagagtgag gtatgaaaat ggcagaccgt agaccactta
     2341 acccgcatgt cgtcgtccct aaaccaggag gcggaactca gccacgagat cgtaataata
     2401 atggaagagt ccgagagaaa cgttcagatg cgggaaaatc aagaaaataa gaaaagccgc
     2461 caaatagggg tcagtttgga tatcggaaac ttttgtacgt taagcgcatt atttaaccaa
     2521 tattgatgcg taagggattg attttacctc agctacgatc gcttttaagt caccgctctt
     2581 tctgtatcgc atttaggtta tccgattaag gaaaattcac gggaaacggc gtatagcgcg
     2641 ggagtacatc attgcgagag gtaagcctgt tcaaaacaca ggcttaccgt acaataattc
     2701 tctatatcca aactgacccc aagaagtctg acactaaccc cacacaaatc gaatccgttg
     2761 caagatgaga cacacaagaa aagagctaga cgaaggtcgc tccgctcccc atggtttttg
     2821 tatacccacc atgggggcat aggtagttta ttaactaact ccaagataat gcgtgatcgc
     2881 agccgtgagt acaccacagc ccacttgttt agataaatct aaaacccctt gtaggattct
     2941 cttagtgttc cactcacctt ctgcatccat tggttgagat agtgcagcac tcaactggtt
     3001 cttaatcttt tgcgaagtta tatcgttatc tattgagttg atttcctcct tggctttctc
     3061 caaggaagcc tggagttcac cgcgttctat ttttaacttt tcacgataac tgtctggtgt
     3121 gtgtagttcg ttcccatccg catcgagatt aataacttcg cctcgctcac caacagtaac
     3181 aaagctctct acgtgagaca gatcatcaac aaccatcaca ttccttttca ttcttaaacc
     3241 agtcactttt gcacctgcac cgatcttcat aaaactaaag cctgatttat cgttcactca
     3301 catcacctca taagataaaa atgttaataa cgttttcacg agcaattatt taccataacg
     3361 cctcattacg cgcaccgctc aaacgctcta gaaacgattc tggggcgtaa tccattaaat
     3421 cctacgtctt accccctgaa aacgcctcaa aatcgctcca gggcgtttta cgggcatgaa
     3481 ttgttatgca ccagactgac tggtggcgga tttcagcctg aattcaggcc agacaactca
     3541 aaacccgcac caaaccagac tgaaaacggt gaacggggac gcaatttatt gcgtggccgt
     3601 gaagcgttgc cggcgcagcc ggaaaaacgg ttttcagcac gttcaggaat cgggttcgtc
     3661 gacggccggc aatcacgtta tgcaagtcgc cggacgactc accacacgat tcaccggcga
     3721 ttcgactcgt gacttgactc tagagtcgcc aaggtcaaaa aaacaccaaa atgacgattc
     3781 gactctagag tcaagtcgtc ataagttact gatagtctta agtgagtcgt cgatgactct
     3841 atgacgagtc gtcggcaagt cgtacacgaa tcgccgacga cttggattca ccttacagaa
     3901 agtaatactg cgtaatgtaa taacccacaa ctaactgata ttaatgaatt atcttcaaaa
     3961 ttttaatttt gcgtggggtg tgggtatttt tagtggtgag gagcgttcag agatctggcg
     4021 atttttggga aggttcagga actccccggg atggccggag aggaaaacgg aagcatgacg
     4081 gagtcaggat tttttccaga atttccacca cggggaatcg ggtggtgaag aaggttcgtg
     4141 cttgtgctcc agaagctgca tagcctgacg aagggaatca atatgctcat ctcgagcctt
     4201 aacagcccct ttaagaccat cgttttcagc ttctagtaaa gcaattctat gttgtaaatc
     4261 atgtaaacca acgctgttaa cacctgtaga tcctgcgtca ctttttacag tatcgatgac
     4321 accatcaaga gaaccaaaaa ctctaataag ctctgaagta tcaataactt taatgatctt
     4381 cccgtgcttt acaacagagg tgtaacttaa cttcccactg ttaatcatct tgtaaattgt
     4441 ggggcgacta accttaacaa gccgtgccgc ttcactaata gacactctgg ccatcagtta
     4501 ccatcctgta
//
