LOCUS       NZ_CP091850             6156 bp    DNA     circular CON 20-DEC-2023
DEFINITION  Klebsiella pneumoniae strain NB05 plasmid pNB05-4, complete
            sequence.
ACCESSION   NZ_CP091850
VERSION     NZ_CP091850.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN25529517
            Assembly: GCF_022059905.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Huang,J. and Li,X.
  TITLE     whole genome sequences of KPC-2- and NDM-1-producing Klebsiella
            pneumoniae isolated from a clinical sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Huang,J. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2022) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/20/2023 03:51:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,848
            CDSs (total)                      :: 5,724
            Genes (coding)                    :: 5,561
            CDSs (with protein)               :: 5,561
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 59 of 163
            Pseudo Genes (incomplete)         :: 106 of 163
            Pseudo Genes (internal stop)      :: 16 of 163
            Pseudo Genes (multiple problems)  :: 16 of 163
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP091850.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NB05"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pNB05-4"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2021-05-11"
     gene            join(<6155..6156,1..622)
                     /gene="aqpZ"
                     /locus_tag="L7D54_RS29100"
                     /pseudo=""
     CDS             join(<6155..6156,1..622)
                     /gene="aqpZ"
                     /locus_tag="L7D54_RS29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226086.1"
                     /GO_function="GO:0015250 - water channel activity [Evidence
                     IEA]"
                     /GO_process="GO:0006833 - water transport [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /name="aqpZ"
     gene            complement(832..1545)
                     /locus_tag="L7D54_RS29105"
     CDS             complement(832..1545)
                     /locus_tag="L7D54_RS29105"
                     /inference="COORDINATES: protein motif:HMM:NF012798.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_071600436.1"
                     /translation="MMDNKPLNKKELESIFLTSGAFNPKGLYLGEGFRKQALELGFPPY
                     ILPTEINIVLHYSPELSVRTFVSTLWNTGASLTEVIHLERKSFVFKSPRPYVKVLSSET
                     ESLSLDNDNRLSPRRKIPLLSPYYIAQVKLLIATLKIAGNTCGDSTKPRRIWDVDNFIA
                     TSWIDNAIKHARLDGVIFPVEVTPDAIKHAFAMNMLILGVEPSIVMKLMGFKRNKWLEI
                     YLKVKCLIMSLERDM"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(1604..2707)
                     /locus_tag="L7D54_RS29110"
     CDS             complement(1604..2707)
                     /locus_tag="L7D54_RS29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="WP_017901372.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEIYNKDGNKLDLYGKIDGLHYFS
                     DDKSVDGDQTYMRIGVKGETQINDQLTGYGQWEYNVQANNVEGSDKQSWTRLAFAGLKF
                     GDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRANGVATYRNSDFFGLVD
                     GLNFALQYQGKNGSVSGEGQTNNGRGWNKQNGDGFGTAITYDIWEGISAGFAYSHSKRT
                     DDQNAAGLVGRGKYAETYTGGLKYDANNIYLAAEYTQTYNATRFSGDSVSGYANKAQNF
                     EVVAQYQFDFGLRPSVAYLQSKGKDIEGFGDQDLLKYVDVAATYYFNKNMSAYVDYKIN
                     LVDDNRFTSAAGISTDDIVALGLVYQF"
                     /name="porin OmpC"
     gene            <2931..3020
                     /locus_tag="L7D54_RS29115"
                     /pseudo=""
     CDS             <2931..3020
                     /locus_tag="L7D54_RS29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901096.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /name="DinI-like family protein"
     gene            complement(3036..3221)
                     /locus_tag="L7D54_RS29120"
     CDS             complement(3036..3221)
                     /locus_tag="L7D54_RS29120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017901373.1"
                     /translation="MSRVTAIISAVVMLNGERVDDDRLQLDTNPLLMDAISAVKYLAGK
                     MEQLQNIIAELKASNA"
                     /name="hypothetical protein"
     gene            complement(3310..>3670)
                     /locus_tag="L7D54_RS29125"
                     /pseudo=""
     CDS             complement(3310..>3670)
                     /locus_tag="L7D54_RS29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383026.1"
                     /GO_function="GO:0003796 - lysozyme activity [Evidence
                     IEA]"
                     /GO_process="GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]; GO:0009253 - peptidoglycan
                     catabolic process [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /name="glycoside hydrolase family protein"
     gene            complement(3746..4777)
                     /locus_tag="L7D54_RS29130"
     CDS             complement(3746..4777)
                     /locus_tag="L7D54_RS29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element ISEc33 family transposase"
                     /protein_id="WP_001752311.1"
                     /translation="MPIIVPIPRGERRLMQKAIHKTRDKNHARRLTAMLMLHRGERVSD
                     VARTLCCARSSVGRWINWFTHSGIEGLKSLPAGRSRRWPFEHICTLLRELIKHSPGDFG
                     YQRSRWSTELLAIKINEITGCQLHAGTVRRWLPSAGLVWRRAAPTLRIRDPHKDEKMAV
                     IHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSG
                     TGKVSYVGGNSKSSALFIALLKHLKATYRRAKTITLIVDNYIIHKSRETQRWLKANPKF
                     RVIYQPVYSPWVNHVEQLWQALHDTITRNHQCRSMWQLLKKVRHFMETASPFPGGKHGQ
                     AKV"
                     /name="IS630-like element ISEc33 family transposase"
     gene            5028..6008
                     /locus_tag="L7D54_RS29135"
     CDS             5028..6008
                     /locus_tag="L7D54_RS29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="WP_000019402.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
                     /name="IS5-like element IS5 family transposase"
ORIGIN
        1 tgcggcagca ttcccgggat taggaattgg ttttgctggg gtggcgctgg ccttcggttt
       61 aacggtatta accatggcat atgccgtcgg tcatatttcc ggcggacatt ttaacccggc
      121 agtgacttta ggtttatggg ctggaggtcg tttcccggcc aaagacgtaa ttggttatat
      181 tatcgcccaa gtcgtcggcg gtattatcgc cgccgctatt ctgtatgttg tcgccagcgg
      241 taaagcgggc ttcgacgcca ccgctagcgg gttcgcttcg aacggttatg gcgaacactc
      301 tccgggcggc ttctcaatgc tttccgccat tgtgatagag attgtcctga catgtggctt
      361 cctgctggtt attcacggtt ccacggataa aagcgctcct gccggcttcg cccctatcgc
      421 cattggactg gcactgacgc ttattcacct gatcagcatt ccggtcacca acacgtcggt
      481 aaacccggcg cgtagcactg cggtagcgat ttttcagggc ggttgggcat tgcaacaatt
      541 atggttattc tgggtcatgc caatcattgg tgggatactg ggcggtttaa tttatcgcac
      601 tttgctcagc agtgataaat gaataattaa aaactctata cgactgcaga tcattccata
      661 ttcacgccag ccatcgaaaa aatcttcagt cctccccact ctgcaggcct tgcatacaac
      721 tgtatgggtg gggagtgggg ggttaaaccg tacaattatc cagcctcgta tagttgatcg
      781 agtgtgtcgc aatttcgagc acctcaacat gatccaaagc atcgcttttt atcacatatc
      841 cctctccaag gacataatta agcatttaac tttcaaatat atttcaagcc acttattcct
      901 tttaaacccc attaacttca ttactattga tggctcaact cccaagatca gcatattcat
      961 tgcaaatgcg tgtttaattg catccggcgt gacttctact ggaaaaatca cgccatccaa
     1021 tctggcatgt ttaatagcat tatctatcca tgatgtcgca atgaaattgt ccacatccca
     1081 tatcctccta ggtttggtgg aatcgccgca agtattaccc gctattttca aagtagcaat
     1141 caataactta acttgagcta tataatatgg ggaaagtaaa ggaattttcc ggcggggaga
     1201 caatctatta tcgttatcta acgacaatga ttcagtttca gaacttaata ctttaacata
     1261 tgggcgagga gatttaaaaa caaaagactt ccgttctaaa tgaatcactt cagttaatga
     1321 agcaccagta ttccataaag tagaaacaaa agttcgtaca ctcaactcag gcgaataatg
     1381 caaaacaatg ttaatctctg tgggtaaaat atatggagga aaacccaatt caagggcctg
     1441 tttacgaaac ccctccccca gatacaaccc cttcggatta aaagcccctg aagtaaggaa
     1501 aatactttca agttcttttt tatttaatgg cttattatcc atcatatctt atccatcata
     1561 tcgacacaaa caccggccac caaggccggt gtttggtaaa taattaaaac tgatatacta
     1621 aaccaagtgc gacaatgtca tcagtagaga tgccagcagc actggtgaaa cgattgtcat
     1681 ccaccaaatt gattttataa tcaacataag cagacatgtt tttgttgaag tagtaggtcg
     1741 cggctacatc aacatatttc agcagatcct gatcgccgaa accttcgatg tctttacctt
     1801 tggactgcag gtatgccacg gacggacgca gaccgaagtc gaactggtac tgtgcaacca
     1861 cttcaaagtt ttgtgcttta ttagcataac cactgacgga atcaccactg aaacgagtcg
     1921 cattataagt ttgggtatac tccgcagcca agtaaatatt gttcgcgtca tatttcaagc
     1981 caccagtgta agtttctgcg tatttacctc gaccaaccag acctgcagca ttctggtcat
     2041 cagttcgttt agaatgagag tatgcaaaac ctgcgctgat gccttcccaa atatcataag
     2101 tgatcgctgt tccgaagcca tcaccgttct gtttgttcca gccgcgacca ttgttagtct
     2161 ggccttcacc acttacacta ccgtttttac cctggtactg cagcgcaaag ttcaggccgt
     2221 caaccagacc gaagaagtca gagttacgat aggttgcaac accattagca cgctgctgca
     2281 tgaagttgtc agagccataa gtgtcaccgc caaactctgg cagaacgtca gtccaggaag
     2341 ttacgtcgta gacaacgccg tagttacgac cgtagtcgaa agagcccacg tcgccgaatt
     2401 tcagaccagc aaatgccaga cgagtccagg actgcttgtc agaaccttca acgttgttcg
     2461 cctgaacgtt gtattcccac tgaccgtagc cagtcagttg gtcattaatc tgggtttcgc
     2521 ccttcacgcc gatacgcatg taggtctgat cgccatcgac gctcttgtca tcagaaaagt
     2581 aatgcaagcc atcaatttta ccgtacaggt ctaatttgtt gccatctttg ttataaattt
     2641 cagccgcatt tgctgcacct gctaccagca gagccggtac caagagggat agtactttaa
     2701 ctttcatggt tgaaccctcg ttttatattt gccactgcgt atcgggctgt cattgtataa
     2761 ttatctatac acaaaaaaat ccagccttct catgctgtaa gctagttaaa agttccctgt
     2821 gaatacacct acattttgta acatattgta aaaggaatgc ataacacgtc atctcgtgtc
     2881 tcataaccaa aacacatagc aataggaatg gacatgccct gaggggctat acagatgcca
     2941 gtaagcatga aaaagaacaa attggcagag ttatcctgga gatgttcgaa gaggctgata
     3001 tgtggatgtc tggagaataa aattagcgat accaatcatg catttgatgc cttcagctct
     3061 gcaatgatgt tttgcaattg ctccattttg ccagctaaat atttcaccgc gctgatcgca
     3121 tccatcagca gcggattggt atcgagttgc aagcgatcat cgtccactct ctcgccgttg
     3181 agcataacca cagcgctgat aatggcggtc acgcggctca tgacatgatt attccaacag
     3241 ccagaaacca cggccacgca tcattaccat ctaaggcaag aaacgcagca atgacgaagc
     3301 aaatcagatt catttcacac catccagaca gagctgcttt tccgctgcgc gacgagtcac
     3361 caggccggga aggatttttc ctccgtcgta taccccagcg tgggaactgg ttgcaggcac
     3421 tagttatctt tccctgtcgc aggtaattaa acagagttga tttctgcata ctggcgcaac
     3481 cagcgttaaa cgtgatcgac gttacagcag aaaatgtatt gtcgcttagt tgtcgtccat
     3541 tgccgtaggt attgacgcag cgctcagccg caaggatgtt tcgctcccgg tctgcggcta
     3601 tctgctaatc agttttgcac acaccgcctt taacgtcatg cgtgttaagg cacagccctg
     3661 atgtaggtag tgattatgct aatagtaacg gacataaaaa agcctgaatc gccacggata
     3721 atctaaatag ctgcgcctaa taccgctaca cttttgcctg accatgtttt cctccgggga
     3781 atgggctggc ggtttccata aaatggcgga cctttttcag taactgccac attgagcggc
     3841 actgatgatt acgggttatc gtgtcatgaa gtgcctgcca tagctgttcc acatgattca
     3901 cccacggcga gtaaaccggc tggtaaatta ccctgaactt gggatttgct ttcaaccagc
     3961 gctgtgtttc gcggctttta tggataatgt agttatcaac gatcagcgtg attgttttcg
     4021 cccgccgata agtggctttc aggtgcttca gcagagcgat aaacagcgct gaacttttgc
     4081 tgttgccgcc cacgtagctg actttacccg tgccactgtg cagtgcgccg gccagatagt
     4141 atttttcgtt ctgccccggc gtcactaccc gtttctgctg tccgcgcaac tgccagtccg
     4201 caccgatttt aggattaagg tggatatcca cttcatcttc ataaaatacc ggatgctctg
     4261 cgctgcattc atccagcgct ttgtggatta ccgccatctt ttcatcttta tgtgggtcac
     4321 ggatacgcag agttggcgcg gccctgcgcc atacaagccc cgcagatggc aaccagcggc
     4381 gaacggttcc tgcatgtaac tggcaaccgg ttatctcatt gatttttatt gccagtaatt
     4441 cggtgctcca gcgtgaacgt tgataaccaa aatcgccggg agaatgcttt atcagctcac
     4501 gtaacagggt gcagatatgt tcaaaaggcc agcgtcggga gcgccctgcg ggtaaggatt
     4561 tcaggccttc aatacctgag tgcgtaaacc agttaatcca gcgaccaacg gatgaacggg
     4621 cacaacagag agttctggca acatcgctga cccgttcacc ccgatgaagc atcagcatgg
     4681 ccgtgagtct gcgggcatga tttttatcac gcgttttatg aatagctttc tgcatcaggc
     4741 gtcgttcgcc acggggtatt ggtactatga tcggcatcgc tcagtccggt tggtgatttt
     4801 ggttggtttg gcgattgatc agatcgcaca atccgggctg agttcccttt cagtgatcta
     4861 ctattccgcg cagctattta gacacttccg agccgttgat aatactggtt ttcatattct
     4921 gtcggtgaca tatgatcgct agaaccatgc cgacgcttag gaaggtgcga acaagtccct
     4981 gatatgagat catgtttgtc atctggagcc atggaacagg gttcatcatg agtcatcaac
     5041 ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa gagattttct
     5101 tgtcccgtat ggagcagatt ctgccatggc aaaacatggt ggaagtcatc gagccgtttt
     5161 atcccaaggc gggcaatggc cgacggccct atccgctgga gaccatgctg cgtattcact
     5221 gcatgcagca ttggtacaac ctgagcgacg gtgccatgga agatgccctg tacgaaatcg
     5281 cctccatgcg cctgtttgcc cgattatccc tggatagcgc cctgccggat cgcaccacca
     5341 tcatgaattt ccgccacctg ctcgagcagc atcaactggc ccgtcaattg ttcaagacca
     5401 tcaatcgctg gctggccgaa gcaggcgtca tgatgaccca aggcactttg gtggatgcca
     5461 ccatcattga ggcacccagc tctaccaaga acaaagagca gcaacgcgat ccggagatgc
     5521 atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg
     5581 ccaagagtgg cctgacccac agcctagtca ccaccgcggc caacgagcat gacctcaatc
     5641 agctgggtaa tctgcttcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag
     5701 gggcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgcc
     5761 ccggcaaggt aagaaccttg aaacagcatc cacgcaagaa caaaacggcc atcaacatcg
     5821 aatacatgaa agccagcatc cgggccaagg tggagcaccc atttcgcatc atcaagcgac
     5881 agttcggctt cgtgaaagcc agatacaagg ggttgctgaa aaacgataac caactggcga
     5941 tgttattcac gctggccaac ctgtttcggg cggaccaaat gatacgtcag tgggagagat
     6001 ctcactaaaa actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt
     6061 caaggcattt acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag
     6121 ccgagaagaa tttccccgct tattcgcacc ttccct
//
